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補中益氣湯과 少陰人 補中益氣湯 및 그 構成藥物群이 高血糖 白鼠의 抗酸化 效果에 미치는 影響
朴宣東,徐敎洙,朴元煥 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2
This study was performed to research the antioxidant activities of Bojungikgitang(補中益氣湯), Soeumin Bojungikgitant(少陰人 補中益氣湯) and its component groups. The experimental group was divided into five groups: Bojungikgitang(B1), Soeumin Bojungikgitang(B2), Bogihyulyak(B0 group), Siho-sengma(B1+) and Gwakhyang-soyeup(B2+). The results were obtained as follows: The level of glucose in control group was considerably increased as much as three times compared with normal group. And in experimental groups, B1 and B2 groups decreased the level of glucose in serum with significancy. And B0, B1+ and B2+ groups also decreased the level of glucose significantly. in vitro; All experimental groups had significant antioxidant activity in linoleic acid system on concentration in comparison with control group, especially B2+ group was higher than any others. In DPPH radical scavenging experiment and inhibitory effect experiment on superoxide generation in xanthine-xanthine oxidase system showed that all experimental groups had some scavenging activity on concentration. In the inhibitory effect experiment on lipid peroxidation reaction in H₂O₂-Fe^2- system showed that each experimental groups had inhibitory effects on lipid peroxidation reaction compared with control group. in vivo; the amounts of lipid peroxide in serum and liver B1 and B2 group were reduced with significancy, and in other groups also were reduced significantly. In hepatic catalase activity test, all experimental groups showed increasing catalase activity significantly but B1 and B2 group with no significancy. In the hepatic glutathione measurement experiment, B1, B2 and B0 groups increased in comparison with control group significantly. And in the activity test of Glutathione- S-transferase, B1, B2 and B0 groups decresed in comparison with control group significantly. As result B1 and B2 groups has exhibited the better antioxidant activities than other groups in vivo. And in component groups, the effects of B0 group were best. These result suggest that in diabetes and antioxidative activity, Bojungikgitant(補中益氣湯) and Soeumin Bojungikgitang(少陰人 補中益氣湯) have strong antioxidant activity with no difference, and it is useful to prevent diabetes which is relevant of peroxidative damage by free radical.
소목(蘇木)이 사람 간암 세포주인 HepG2의 세포사멸에 미치는 영향과 그 경로
김판준,윤현정,이영태,서교수,박선동,Kim, Pan-Jun,Yun, Hyun-Joung,Lee, Young-Tae,Seo, Kyo-Soo,Park, Sun-Dong 대한한의학방제학회 2005 大韓韓醫學方劑學會誌 Vol.13 No.2
The purpose of this study was to investigate the anticancer effects of Caesalpiniae Lignum (Somok) on HepG2 cells, a human liver cancer cell line. To study the cytotoxic effect of Caesalpiniae Lignum methanol extract (CL-MeOH) on HepG2 cells, the cells were treated with various concentrations of CL-MeOH and then cell viability was determined by XTT reduction method and trypan blue exclusion assay. CL-MeOH reduced proliferation of HepG2 cells in a dose-dependent manner. To confirm the induction of apoptosis, HepG2 cells were treated with various concentrations of CL-MeOH. The activation of caspase 3 and the cleavage of poly ADP-ribose polymerase (PARP), a substrate for caspase-3 and a typical sign of apoptosis, was examined by western blot analysis. CL-MeOH decreased procaspase 3 level in a dose-dependent manner and induced the clevage of PARP at concentration> $200{\mu}/ml$. Mitogen-activated protein (MAP) kinase signaling cascades are multi-functional signaling networks that influence cell growth, differentiation, apoptosis, and cellular responses to stress. CL-MeOH-induced MAPK activation was examined by Western blot for phosphorylated ERK, p38 and JNK. CL-MeOH significantly increased p38 phosphorylation and JNK phosphorylation in a dose-dependent manner. Inhibition of p38 function using the selective inhibitor SB20358O results in inhibition of apoptosis by CL-MeOH. These results suggest that CL-MeOH-induced apoptosis is MAP kinase-dependent apoptoric pathway. These results suggest that CL-MeOH is potentially useful as a chemotherapeutic agent in human liver cancer.