백두옹 분획층의 항산화 효과

박선동 ( Sun Dong Park ),조현진 ( Hyun Jin Cho ),윤현정 ( Hyun Jeong Yun ),이효승 ( Hyo Seung Yi ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.2

Objectives: This study was designed to investigate the effects of anti-oxidation of fractionated Pulsatilla koreana NAKAI (PK) extracts. And we examined to determine that a certain fractionated extract has the best anti-oxidative effects between the fractionated PK extracts. Methods: Anti-oxidative effects of fractionated PK extracts was measured by scavenging activities of DPPH, superoxide, nitric oxide (NO) and peroxynitrite radicals. And also scavenging activities of anti-oxidation in lipopolysaccharide (LPS)-treated RAW 264.7 cell was measured. After these examination, we determined a fraction that has best anti-oxidative effects. Results: Fractionated PK extracts inhibited radicals effectively. Also in RAW 264.7 cell, intracellular oxidation has inhibited by PK extracts. In these tests, ethyl acetate (EA) fraction has the best anti-oxidative effects among PK extracts. Conclusions: This results demonstrate that PK extracts exhibit anti-oxidative effects. And EA fraction has the best inhibition effects among the six fractions of PK.

마우스대식세포주인 RAW 264.7에서 SD-01의 항염증 활성 연구

박선동 ( Sun Dong Park ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.3

Objective: The aim of this study was to investigate anti-inflammatory activity of SD-01 methanol extract in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods: Cytotoxic activity of SD-01 methanol extract on RAW 264.7 cells was measured using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines and PGE2 were measured by ELISA method. The levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), I k-B-alpha and nuclear NF-k B p65 expression were detected by western blot. Results: Our results indicated that methanol extract of SD-01 significantly inhibited the LPS-induced NO, PGE2 production and iNOS, COX-2 expression accompanied by an attenuation of TNF-a, IL-1b, IL-6 and MCP-1 production in RAW 264.7 cells. Moreover, methanol extract of SD-01 treatment also blocked LPS-induced NF-k B activation. Conclusion: These findings indicate that methanol extract of SD-01 inhibits the production of pro-inflammatory mediators and cytokines via suppression of NF-k B activation. Take together, these results indicate that methanol extract of SD-01 has the potential for use as an agent of anti-chronic inflammatory diseases.

마우스대식세포주인 RAW 264.7에서 金鈴子散(金鈴子散)의 항염증 활성 연구

박선동 ( Sun Dong Park ),김도형 ( Do Hyung Kim ),이효승 ( Hyo Seung Yi ),윤현정 ( Hyun Jeong Yun ),차창민 ( Chang Min Cha ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.2

Objective: The aim of this study was to determine whether methanol extract of Keum-Ryung-Ja-San (KRJS) inhibit production of NO, PGE2, iNOS, COX-2 and pro-inflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Methods: Cytotoxic activity of extracts on RAW 264.7 cells was measured using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines and PGE2 were measured by ELISA kit. The levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), Ik-B-a and nuclear NF-k B p65 expression were detected by western blot. Results: Our results indicated that methanol extract of KRJS significantly inhibited the LPS-induced NO, PGE2 production and iNOS, COX-2 expression accompanied by an attenuation of TNF-a, IL-1b and IL-6 production in RAW 264.7 cells. Moreover, methanol extract of KRJS treatment also blocked LPS-induced NF-k B activation. Conclusion: These findings indicate that methanol extract of KRJS inhibits the production of pro-inflammatory mediators and cytokines via suppression of NF-k B activation. Take together, these results indicate that methanol extract of KRJS has the potential for use as an agent of anti-chronic inflammatory diseases.

마우스 대식세포에서 도기탕 (導氣湯) 메탄올 추출물의 항산화 및 항염증 효과

박선동 ( Sun Dong Park ),김동완 ( Dong Wan Kim ),윤현정 ( Hyun Jeong Yun ),허준영 ( Jun Young Heo ),김태훈 ( Tae Hoon Kim ),조현진 ( Hyun Jin Cho ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.4

Objective: The aim of this study was to determine whether methanol extract of Do-Ki-Tang (DKT) inhibit free radical generation and production of nitrite an index of NO, PGE2, iNOS, COX-2 and pro-inflammatory cytokines such as TNF-a, IL-1b, IL-6 and MCP-1 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods: Cytotoxic activity of extract on RAW 264.7 cells was measured using 5-(3-caroboxymeth -oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The expression level of inflammatory response-related proteins was confirmed by western blot. The production of proinflammatory cytokines was measured by ELISA. Results: Our results indicated that DKT scavenged DPPH radical and nitric oxide in vitro. Moreover, DKT significantly inhibited the LPS-induced NO, PGE2 production and iNOS, COX-2 expression accompanied by an attenuation of TNF-a, IL-1b, IL-6 and MCP-1 formation in macrophages. Furthermore, DKT treatment also blocked LPS-induced intracellular ROS production and the activation of NF-kB and MAPKs. Conclusion: Our data suggest that the anti-inflammatory effect of DKT is mediated through down-modulation of pro-inflammatory mediators and cytokines by blocking the signaling pathways of NF-kB and MAPKs. These inhibitory effects by DKT represent a potential therapeutic approach to the treatment of inflammatory diseases.

GGEx18의 ethyl acetate 분획물에 의한 고지방식이 비만 마우스의 식이효율과 혈중 leptin 농도에 미치는 영향

박기정,이희영,이혜림,윤미정,박선동,이용태,심지빈,최홍화,신순식,Park, Ki-Jeong,Lee, Hee-Young,Lee, Hye-Rim,Yoon, Mi-Chung,Park, Sun-Dong,Lee, Yong-Tae,Shen, Zhi-Bin,Cui, Hong-Hua,Shin, Soon-Shik 대한한의학방제학회 2011 大韓韓醫學方劑學會誌 Vol.19 No.1

Objectives : This study was designed to determine the effects of the GGEx18 ethyl acetate fraction(EF) on body weight gain, feeding efficiency ratio, and obesity-related factors in plasma as well as histology of liver and adipose tissues using high fat diet-fed male C57BL/6N obese mice. Methods : 8 weeks old, high fat diet-fed obese male mice were divided into 5 groups: C57BL/6N normal, control, EF(1), EF(2) and EF(3). After mice were treated with EF for 9 weeks, we measured body weight gain, food intake, feeding efficiency ratio, fat weight, plasma leptin and lipid levels. We also analysed histology of liver and adipose tissues on high fat diet-fed male C57BL/6N obese mice. Results : Compared with control, EF-treated mice had significantly lower body weight gain and feeding efficiency ratio. Consistent with the effects on body weight gain, EF significantly decreased the adipose tissue weight compared with control. Consistent with the effects on feeding efficiency ratio, EF significantly decreased plasma leptin concentrations compared with control. EF reduced the size of adipocytes as well as hepatic lipid accumulation compared with control. EF seems to be safe since not only the plasma levels of ALT and AST are within the normal range, but also EF did not show any toxic effects on organs. EF(3) was most effective among EF(1), EF(2), and EF(3) at doses of 25, 50, and 100 mg/kg, respectively. Conclusions : These results demonstrate that EF effectively reduces body weight gain, feeding efficiency ratio in high fat diet-fed obese mice, leading to the modulation of obesity. In addition, EF decreases the size of adipocytes and improves plasma lipids and controls hepatic lipid accumulation, suggesting that EF may act as a therapeutic agent for obesity.

혈부축어탕(血府逐瘀湯)과 그 구성약물군(構成藥物群)이 Alloxan 유도 당뇨(糖尿) 백서(白鼠)의 간독성에 미치는 영향

박선동,Park Sun-Dong 대한한의학방제학회 2002 大韓韓醫學方劑學會誌 Vol.10 No.1

This study was performed to observe the effect of hyulbuchukeo-tang and its component groups on recovery of hepatoxicity in alloxan treated rats. The experimental group was divided into 3 groups: hyulbuchukeo-tang(HCT) and its components groups, sayeok-san(H-sa) and dohong-samul-tang (H-do). The results were obtained as follows: 1. The level of Glucose was considerably reduced by HCT, H-sa, H-do with high significancy. 2. In the change of GOT activity, HCT and H-do were decreased with high significancy, H-do was decreased with some significancy. 3. In the change of GPT activity, HCT was decreased with high significancy, H-sa and H-do were decreased with some significancy. 4. In the change of ${\gamma}-GPT$ activity, HCT was decreased with high significancy, H-do was decreased with some significancy. 5. In the change of ALP activity. only HCT was decreased with some significancy. 6. In the change of LDH activity, HCT was decreased with high significancy, H-sa and H-do were decreased with some significancy. 7. In the change of bilirubin contents. only HCT was decreased with some significancy. As result, hyulbuchukeo-tang(HCT) has significant effects on recovery of hepatoxicity, and its components groups. sayeok-san(H-sa) and dohong-samul-tang(H-do) have some effects. It suggests that sayeok-san(H-sa) and dohong-samul-tang(H-do) have more effects on recovery of hepatoxicity in case of the combination of the two (that is hyulbuchukeo-tang) than in case of the separation.

동의보감(東醫寶鑑) 외형편(外形篇) 권(卷)1 [두(頭)]부분 관련처방의 재해석

박선동,Park, Sun-Dong 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.1

許浚(Heo Jun), the best physician on his day, wrote 東醫寶鑑(DongEuiBoGam, 1613) based on his clinical experience as a court doctor and life-long study of over 500 medical books. Now most clinicians consult 東醫寶鑑(DongEuiBoGam) for its practicality. And it is also considered as one of the most valuable medical books for basic medical studies. As 許浚(Heo Jun) mentioned on the 東醫寶鑑(DongEuiBoGam) preface. we should practice flexibility on the interpretation of the medical classics instead of binding ourselves to the precedents. But worship of 東醫寶鑑(DongEuiBoGam) may hinder the studying of new pathways. The treatment and its understandings of a disease should be based on objectivity of observation. Finding the common traits of the recipes in 東醫寶鑑(DongEuiBoGam) is important for the study of 東醫寶鑑(DongEuiBoGam). Accordingly, group synthesis of symptoms and resultant classification of recipes are vital. In this research, symptoms listed in the head part(頭門) of External Shape vol. I(外形篇 卷1) of 東醫寶鑑(DongEuiBoGam) are synthesized into 3 different parts: head-wind syndrome(頭風證), dizziness(眩暈), headache(頭痛). Classification of prescriptions according to these 3 parts is also suggested.

월국환(越鞠丸) 물 추출물이 Acetaminophen으로 유도된 마우스의 급성 간손상에 미치는 효과

이채중,박선동,문진영,Lee Chae-Jung,Park Sun-Dong,Moon Jin-Young 대한한의학방제학회 2003 大韓韓醫學方劑學會誌 Vol.11 No.2

Objectives : Wolguk-whan has been used as a prescription of natural drug for the treatment of stress digestive system disease. Recently, we reported that Wolguk-whan methnol extract (WGWM) exerted a significant protective effect against oxidative damage to the liver of ICR mice. This study was purposed to investigate the effects of Wolguk-whan water extract (WGWW) on liver injury induced by oxidative stress. Methods : In order to investigate the effects of WGWW on acute liver injury, ICR mice were pretreated with WGWW for 6days, starved for 24hrs, and administerated acetamirtophen(500mg/kg, i.p.). In the liver homogenates, lipid peroxide and glutathione(GSH) levels were measured. In addition, activities of hepatic enzyme, such as catalase, glutathione peroxidase(GSH-Px), glutathione S-transferase(GST) were measured in the hepatic mitochondrial and cytosolic fractions. Results : In vivo administeration of WGWW showed effective inhibition of acetaminophen induced lipid peroxidation, and showed elevations of GSH level, catalase, GSH-Px, GST activities. Conclusions : These results suggested that WGWW might suppress the formation of oxidative metabolites, and prevent acetaminophen induced hepatotoxicity.

금궤요략의 상견복증(常見腹證)에 관한 연구(硏究)

홍문엽,박선동,박원환,Hong, Mun-Yeup,Park, Sun-Dong,Park, Won-Hwan 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

금궤요략은 후한말기(後漢末期) 장기(張機)가 지은 임상의학(臨床醫學) 전문서적(專門書籍)으로써, 리(理) 법(法) 방(方) 약(藥)이 갖추어진 독창적(獨創的)인 변증론치체계(辨證論治體系)를 수립하고 있으며, 특히 복증(腹證)에 관한 내용을 중(重)히 다루고 있고, 방증변증(方證辨證)이 중심(中心)이 되어 복증(腹證)을 통한 변증(辨證)이 매우 발달되어 있어서 진단학(診斷學)의 발전(發展)에 크게 기여하였다. 이후 진단학(診斷學)의 발전(發展)은 진맥(診脈), 진설(診舌)을 위주로 했으며, 복진(腹診)의 운용(運用)에 대해서는 역사적으로 사회적인 특수한 배경으로 인하여 계속 발전되지 못하였다. 최근 한의학적(韓醫學的) 진단방법(診斷方法)과 치료방법(治療方法)이 매우 강조되어 활발한 연구가 계속 진행되면서 복증변증(腹證變證)에 관한 관심이 매우 집중되고 있기에, 복부진단(腹部診斷)에 관한 연구(硏究)의 한 방법(方法)으로써 금궤요략에 실려있는 상견복증(常見腹證)에 관련된 내용(內容)을 정리한 결과(結果) 약간의 지견(知見)을 얻었다. The subject of Abdominal syndrome in the field of Jin Kui Yao Lue takes a quarter of the whole research quantity, and has greatly contributed to the development of diagnoses due to the well-growth of syndrome differentiation through 'the differentiation of symptoms and signs based on prescriptions'-centered abdominal syndrome. Since then, while the diagnostic has been developed mainly in the field of pulse feeling and the inspection of the tongue, application of the abdominal diagnostic has not been continuously improved because of special historical and social environments. Recently, since interest in the differentiation of symptoms and signs based on abdominal syndrome has been raised by emphasis of Oriental diagnostic methods and. medical treatment which have led to the study of Abdominal Syndrome in Jin Kui Yao Lue. The following is the results of the study. 1. Jin Kui Yao Lue abdominal syndrome is categorized into all abdominal symptoms Epigastric symptoms, Hypochondrium symptoms, and Lower abdomen symptoms. 2. Subjective symptoms and Objective symptoms have been found in Abdominal syndrome, and Subjective symptoms have been more often than Objective symptoms. Both of the symptoms have been found more to co-exist in abdominal syndromes. 3. More cases of fullness of abdomen symptoms in All abdominal symptoms, a smaller number of cases in Taiyang disease, Yangming disease, disease involving all three yang, Tayin disease and Jueyin disease have been found, but there have not been found in Shaoyin disease. 4. More cases of Epigastric fullness and rigidity in Epigastric symptoms, Epigastric throbs in Palpitation symptoms, and sense of fullness-in-chest in Abdominal syndrome of chest and hypochondrium have been recognized. 5. Any regularity caused by abdominal symptoms has not been identified. 6. Diagnosis of the abdomen caused by abdominal symptoms has been identified in Epigastric fullness, Epigastric pain, Epigastric procrastination, Epigastric throb, fullness of abdomen and distension of lower abdomen.

시호사물탕이 t-BHP로 유도된 간세포의 산화적 손상 및 자유기에 의한 지질과산화 반응에 미치는 영향

김태기,박선동,문진영,Kim Tae-Gi,Park Sun-Dong,Moon Jin-Young 대한한의학방제학회 2000 大韓韓醫學方劑學會誌 Vol.8 No.1

Sihosamultang(SST) has been used for the treatment of puerperal fever, liver disease in traditional medicine. The present study was carried out to evaluate the antioxidant effects of SST extract in vitro. The inhibitory effect of SST extract on lipid peroxidant was examined in the linoleic acid autoxidation system. In this test, SST extract significantly inhibited the time course of the lipid peroxidation. And SST extract showed about 73% scavenging effect on DPPH radical. And this extract inhibited not only the lipid peroxide formation induced by hydroxyl radical derived from $ H_{2}O_{2}-Fe^{2+}$ in the rat liver homogenate, but also the superoxide generation from xanthine-xanthine oxidase system in a dose-dependent manner. In addition, SST extract protected the hepatic cell death induced by tert-butyl hydroperoxide. These data indicated that SST might play a protective role against oxidative injury by free radicals.