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      • 이온성액체를 이용한 고생산성 단백질 재접힘 공정 개발

        배상우 인하대학교 대학원 2014 국내박사

        RANK : 247631

        Recently, expiration of patents for major drug products and advancement of genetic recombinant technology have facilitated the production of protein for drugs and other valuable products via microorganisms. However, there are occasions where organelle responsible for folding in over-expressed foreign recombinant proteins does not function properly. Eventually, these lead to production of recombinant protein in the shape of protein aggregates called inclusion body due to the flocculation of the hydrophobic patches of protein. Consequently, protein refolding process is essential to convert such inactive inclusion body protein into that of active form, which have correctly folded tertiary structure. But protein refolding process in industrial protein purification steps is generally accepted as a low efficiency process. There are many attempts to improve the existing low efficiency refolding process. Meanwhile, ionic liquids (ILs) are molten salt, which is composed of ions (anion and cation) having melting points below 100℃. Recently, various advantages of ILs, including low toxicity, non-volatile, and possibility of various combinations of anion and cation, have facilitated its application to many industrial processes. For example, ILs have been widely used in various enzymatic reactions as a solvent for enhanced enzyme activity and stability. ILs can also be used as a refolding enhancer in refolding process. In this study, we suggest IL-assisted highly productive protein refolding process to overcome the low efficiency in existing refolding. Herein, we elucidate 1) the effects of imidazolium-based ILs on refolding yield of the model protein upon their structural characteristics, 2) compatibility of the imidazolium-based ILs as refolding enhancer with inclusion body protein produced microbially by applying the result obtained from the model protein refolding, and 3) propose a method for IL recovery from refolding solution. To investigate the effects of imidazolium-based ILs on the refolding of model proteins according to their structural characteristics, we observed refolding yield based upon the presence/absence of disulfide bonds or metal cofactors in their tertiary structure. For model proteins, we used lysozyme which has four disulfide bonds, laccase which requires copper as a cofactor, and horseradish peroxidase (HRP) which has two calcium ions and ferrous ion in the heme group as cofactors. As a result, sulfur-containing methylsulfate ([MS]-), copper containing trichlorocuprate ([CuCl3]-), and chloride ([Cl]-) anions showed outstanding effects as a refolding enhancer for lysozyme, laccase, and HRP refolding, respectively. Moreover, we observed the effects of refolding time, ILs concentration, and the refolding temperature on the refolding yield of each model protein. Model protein refolding yields in each optimum refolding buffer containing ILs were about 100%, 91%, and 81% which were 2.9, 2.7, 3.5 times higher than that in the urea-containing conventional refolding buffer for lysozyme, laccase, and HRP refolding, respectively. In the investigation of effects of ILs concentration on the model protein refolding yields, we also observed that protein aggregates were formed only below a certain concentration of ILs, but protein aggregate formation was prevented with the presence of higher ILs concentration. Consequently, we could increase model protein refolding yield, and verified the effect of ILs on the suppression of aggregate formation during refolding process. In addition, we attempted to verify the applicability of IL as refolding enhancer to the industrial refolding process by using inclusion body protein. Transforming growth factor beta 3 (TGF-β3) derived from E. coli host was used as refolding model protein. Refolded TGF-β3 was qualitatively analyzed via SDS-PAGE. As a result, in accordance with previous research, when ionic liquid including [MS]- anions was added, an increase of TGF-β3 refolding efficiency was observed. In sum, we investigated the strategy to recovery of ILs used for refolding enhancer from refolded protein solution. In the recovery of ILs using dialysis membrane, we confirmed 95% of ILs recovery yield via RP-HPLC analysis, and activity of refolded enzyme also remained high during dialysis. In conclusion, we propose the strategy to overcome conventional low-efficient and low-productive refolding process by application of ILs to protein refolding process. Such highly productive protein refolding methods will facilitate the enhanced recombinant protein productivity in microorganisms.

      • (A)Study on the 3D Printing Materials using Wood-derived Cellulose : From Bio-plastics to Photopolymers

        배상우 국민대학교 일반대학원 2020 국내석사

        RANK : 247631

        3D printing refers to any technology for manufacturing a real object by laminating proper materials according to three-dimensional design data. It is a technology to design an object to be a virtual object through a three-dimensional digital drawing, and then stack each material along a design shape in a very thin section to form an object. Therefore, it's getting great attention nowadays as a very efficient technical alternative for a model that is difficult to implement with a conventional manufacturing method or a product that can be produced only through a complicated and numerous process. There are many ways to execute 3D printing technology. Among them, the most widely used methods are a fused deposition modeling (FDM) in which thermoplastic resins are melted and laminated, and a digital light processing (DLP), which selectively cures photo curable resins by irradiating light energy source in a short wavelength band. However, conventional materials used in these methods need to be developed and supplemented in terms of range, usability, and the physical properties of the end products. In addition, the research is not only needed to improve various functionalities of 3D printed object, but the approaches on plastic wastes used in FDM and DLP is also necessary in view of environmental pollution which is a significant part of modern society. In this study, bioplastics and wood-derived cellulose resources (i.e., wood flour and cellulose nanocrystals) were used to develop the novel materials used for 3D printing, and composites were prepared using functional fillers (i.e., nanoclay and nano-CaCO3) and evaluated for various properties. For the filament form material used in the FDM, wood flour was added based on PLA, a representative bioplastic, and optimize 3D printing parameters according to the amount of wood flour addition, and composites were manufactured to evaluate various properties. The morphological properties were investigated to determine the mechanism of voids generation in 3D printed object by the addition of wood flour, and its effects on the mechanical properties. In addition, by examining the biological degradation properties according to the addition of wood flour of bioplastics/wood flour composite material by microorganisms, it was confirmed that it represents a better eco-friendly properties according to the amount of wood flour added. Based on the experimental results, the optimum amount of wood flour (20 wt%) was selected for PLA polymer, and the possibility of introducing 3D printing of PBAT, another bioplastic, was investigated according to the optimized 3D printing parameters. The morphological and mechanical properties according to the PBAT additions were investigated, and the results showed that the thermal stability was significantly improved by the addition of PBAT. Therefore, filament manufactured from PLA and PBAT, which are eco-friendly bioplastics, and wood flour, which is a natural and sustainable resource, is highly applicable as a new resource-cycling material for FDM 3D printing. In addition, wood flour incorporation induced the cost-effectiveness, and visual appearance in terms of external aesthetics is successfully improved. The composite material of cellulose nanocrystals and functional fillers was prepared by 3D printing on the liquid photocurable resin used in the DLP, and various properties were analyzed. The influence to morphological characteristics and mechanical strength of composites was investigated by examining the mechanism of freezing behavior of CNC according to the lyophilization concentration of CNC added to the composites, and the pretreatment process step for the addition of CNC and inorganic fillers to the photocurable resin was established. The optimum amount of CNC addition was investigated by analyzing the mechanical and morphological properties according to the concentration of CNC addition. In addition, the composites were fabricated to show better physical properties through the addition of nanoclay and nano calcium carbonate. Through the research of manufactured composites not only studied the utilization of wood-derived cellulose resources, but also showed better mechanical properties compared to conventional photocurable resins, and therefore, it can be applied as a functional new material applicable to DLP 3D printing. The results obtained through the above studies are considered to be precedence study that uses various forest resources for FDM and DLP 3D printing materials to build a basic property information, and develop eco-friendly materials with better properties that can be applied to 3D printing. It is expected that this will lay the foundation for future research on 3D printing materials. 3D 프린팅(3D Printing)이란 3차원 설계 데이터에 따라 고유의 소재를 적층함으로써 실제 물체를 제조하는 기술 일체를 의미한다. 제작하고자 하는 물체를 3차원 디지털 도면을 통해 가상의 모델로 설계한 후, 각 소재를 매우 얇은 단면으로 한 층(layer)씩 쌓아 실체를 형성하게 된다. 따라서 기존의 제조 방식으로는 구현하기 어려운 모형이나 복잡하고 많은 공정을 거쳐야만 생산이 가능한 대상에 대해 매우 효율적인 기술 대안으로 주목받고 있다. 3D 프린팅 기술을 구현하는 방식은 다양한데, 그 중에서 가장 널리 사용되는 방식은 열가소성 수지를 용융하여 적층하는 FDM(fused deposition modelling)과 광경화 수지에 짧은 파장대의 빛 에너지를 조사하여 선택적으로 경화하는 DLP(digital light processing)가 존재한다. 하지만 이러한 방식들에 사용되는 기존의 소재들은 종류의 범위나 사용성, 그리고 그 최종 결과물의 물성에서 개발과 보완의 여지가 남아있다. 또한 현대사회에서 상당한 부분을 차지하고 있는 환경오염 문제의 측면에서 FDM과 DLP 방식에 사용되는 플라스틱 폐기물들에 대한 방안을 모색하여 환경적 측면을 고려함과 동시에 3D 프린팅 출력물의 다양한 기능적 향상을 도모하는 연구가 필요하다. 이 연구에서는 3D 프린팅에 사용되는 재료의 개발 및 기능 강화를 위해 바이오 플라스틱과 목질 유래 셀룰로오스 자원을 활용하고, 기능성 첨가제를 첨가하여 복합재를 제조하고 다양한 물성을 평가하였다. FDM방식에 사용되는 필라멘트 형태의 재료에 대하여 대표적인 바이오 플라스틱인 PLA를 기반으로 목분을 첨가하여 목분 첨가량에 따른 3D 프린팅 출력 매개변수를 최적화하고, 복합재를 제조하여 다양한 물성을 평가하였다. 형태학적 특성을 조사하여 목분의 첨가에 따른 공극 발생의 기작을 구명하였으며 그에 따른 기계적 강도 특성에 미치는 영향을 조사하였다. 또한, 미생물에 의한 바이오 플라스틱/목분 복합재의 목분 첨가에 따른 생물분해 특성을 조사하여 목분이 첨가량에 따른 친환경 특성을 갖는 것을 확인하였다. 조사된 실험 결과들을 통해 PLA 고분자에 대한 목분의 최적 첨가량(20 wt%)를 선정하였으며, 최적화된 출력 매개변수에 따라 또다른 바이오 플라스틱인 PBAT의 3D 프린팅에 대한 도입 가능성을 조사하였다. PBAT 첨가량에 따른 형태학적 특성과 기계적 특성을 조사하였으며, 특히 PBAT 첨가에 따라 열 안정성이 뛰어나게 개선되는 결과를 확인하였다. 따라서 친환경 바이오 플라스틱인 PLA와 PBAT, 그리고 천연자원이자 지속 가능한 자원인 목분을 통해 제조된 필라멘트는 FDM 3D 프린팅에 적용 가능한 자원 순환적인 신소재로서 적용 가능성이 큰 것으로 판단되며, 목분의 첨가로 인한 비용 절감의 효과 및 심미적 측면의 개선 또한 이루어 진 것으로 판단된다. DLP 방식에 사용되는 액상의 광경화 수지의 재료에 대하여 셀룰로오스 나노크리스탈과 기능성 첨가제를 첨가한 복합재를 3D 출력하여 제조하고 다양한 특성을 분석하였다. 제조된 복합재에 첨가되는 CNC의 동결건조 농도에 따른 CNC의 동결 거동 기작을 조사함으로써 형태학적 특성 및 복합재의 기계적 강도에 미치는 영향을 조사하였으며, 광경화 수지에 CNC 및 무기 첨가제의 첨가에 대한 전처리 공정 단계를 확립하였다. CNC 첨가 농도에 따른 기계적 특성과 형태학적 특성을 조사하여 최적의 CNC 첨가량을 구명하였으며 이에 나아가, 나노클레이와 나노 탄산칼슘의 첨가를 통해 더 나은 물성을 나타낼 수 있는 복합재를 제조하여 그 특성을 조사하였다. 제조된 복합재에 대한 연구를 통해 목질 유래 셀룰로오스 자원의 활용 가능성을 검토하였을 뿐만 아니라 기존의 광경화 수지와 비교하여 더 나은 기계적 특성을 나타내어 DLP 3D 프린팅에 적용 가능한 기능성 신소재로서 적용될 수 있을 것으로 판단된다. 위의 연구들을 통해 얻어진 결과는 FDM 및 DLP 3D 프린팅 소재에 대해 임산 자원을 활용한 선행 연구로써 다양한 기초 물성 정보를 구축하였으며, 3D 프린팅에 적용될 수 있는 더 나은 물성을 지니며 동시에 친환경적인 재료를 개발함으로써 추후 3D 프린팅 소재 연구의 토대를 마련할 수 있을 것으로 기대된다.

      • 직접 패턴 PZT 박막의 특성 및 micro-cantilever 제작 응용 연구

        배상우 연세대학교 대학원 2005 국내석사

        RANK : 247631

        MEMS라는 초소형 정밀기계 시스템에 PZT 박막의 응용 시 야기 되는 건식 식각 공정에 따른 물리·화학적 손상의 문제점을 보완하기 위해 감광성 물질인 NBAL이 첨가된 sol-gel 방법을 이용하여 직접 패턴이 가능한 PZT 박막을 제작하였다. 먼저 micro-device로의 적용 특성을 높이기 위해 La 가 도핑 된 PLZT 박막을 제작하여 이에 따른 특성을 연구하였다. 측정 결과 La 도핑 몰수가 증가 할수록 특히 4 mol% 첨가 시 누설 전류 특성과 피로 특성이 증가 하였다.직접 패턴에 따른 물성 변화를 측정하기 위해 3회 다층 패턴을 통한 500 nm 두께의 460 x 460 μm 크기로 패턴 된 PZT(5248) 박막을 제작하여 실제 micro device로의 응용가능성을 확인하였다. 다층 패턴에 따른 박막의 형상의 변화는 발생하지 않았으며 열처리 후의 박막의 수축만 발생하였다. 직접 패턴 된 PZT 박막의 경우 패턴 되지 않은 박막과 거의 유사한 물성이 발현됨을 확인 하였다. 이를 바탕으로 하여 MEMS 공정에 의해 SiNx 지지층 위에 SiO2TaPtPZTPt의 다층 박막을 갖는 직접 패턴 PZT 마이크로 캔틸레버를 제작하였으며 캔틸레버 길이에 따른 공진 특성 및 인가전압에 따른 tip deflection을 분석하였다. 이 경우 캔틸레버 길이 L-2에 비례함을 확인 할 수 있었으며 인가전압이 증가 할수록 tip deflection은 증감함을 확인하였다. Au mass loading을 통해 100 x 200 μm 크기의 직접 패턴 PZT 마이크로캔틸레버의 감도를 측정한 결과 38.231 Hzng로 측정되었다.마지막으로 PZT 졸 구성 요소 및 열처리 후의 냉각 방법을 변화시켜 박막의 스트레스 완화를 유도하였다. Raman spectroscopy를 통해 기존의 직접 패턴 PZT 박막과 완화 유도된 PZT 박막의 스트레스를 비교하였으며 stress 완화된 직접 패턴 PZT 박막의 경우 micro-detecting system에 응용 가능한 스트레스 값을 나타내었다. Direct-patternable lanthanum substituted lead zirconate titanate(PLZT) films were propared by sol-gel technique using orth-nitrobenzaldehyde as photosensitive agent. PLZT films with various La concentration were formed on Pt(111)TiSiO2Si(100) substrate for direct-patterning of PLZT films and ferroelectric properties measurement. A well-defined and direct-patterned PLZT film was obtained and improvement of leakage current density, fatigue resistance and dielectric constant with increased substitution of La in PLZT film were observed especially with 4 mol% La doped PLZT film.Ferroelctric properties of direct-patterned PZT(PbZr0.53Ti0.48O3) film with 460 μm x 460 μm size and 510 nm thick were analyzed for applying to micro-detecting devices. A photosensitives solution containing orth-nitrobenzaldehyde was used for the preparation of direct-patterned PZT film. PZT solution was coated on Pt(111)TiSiO2Si(100) substrate for 3 times to obtain a half micron thick film and 3 times of direct patterning process were repeated to define a pattern on mult-layer PZT film. Through intermediate and final anneal procedure of direc-patterned PZT film, any shrinkage along horizontal direction was not observed within this experimental condition, i.e., the size of the pattern was preserved after annealing, only a thickness reduction was observed after each annealing treatment. Ferroelectric properties of direct-patterned PZT film with 460 μm x 460 μm size and 510 nm thick were compared with those of un-patterned conventional PZT film and shown to be almost the same.This work is the first demonstration of the fabrication of direct-patternable PZT microcantilever for the application of micro-detectiong system using frequency shift. A micromachined direct-patterned PZT thin film cantilever composed of SiO2TaPtPZTPt on SiNx supporting layer for sensing was fabricated. Electromechanical characteristics of micromachined direct-patterned PZT cantilever were measured by laser vibrometer system as a function of cantilever length. The electromechanical properties such as resonant characteristics with various cantilever length and displacement with various voltage were investigated respectively. The gravimetric sensitivities of cantilever in air were characterized by Au deposition on the backside of cantilever. The gravimetric sensitivity of 100 x 200 μm dimension cantilever was 38.231 Hzng.Finally, a development of stress in the film was investigated by modifying the anneal condition and the constituents of PZT sol. The residual stress in the conventional and modified direct-patternable PZT films was investigated by the Raman spectroscopy. In addition, ferroelectric properties of modified PZT film was compared with those of conventional film. Finally, we asses the applying feasibility of direct-patternable PZT film to micro-detecting system.

      • 전착법을 이용한 PbTe의 성장 및 성장기구

        배상우 경북대학교 대학원 2016 국내석사

        RANK : 247631

        PbTe was gradually studied to understand to growth method, crystal structure, and physical properties. In this study, electrodeposition of the PbTe was conducted with 3 electrode method. ITO glass and Pt foil used as working and counter electrode. Reference electrode was Ag/AgCl(saturated KCl) electrode. PbTe was potentiostatically electrodeposited and the effect of Te/Se ratio on Pb-Te-Se thin film were investigated at various composition. Binary PbTe and ternary PbTeSe were fabricated by electrodeposition, and the PbTe morphology was dendrite shape and microstructure was FCC single crystal. Through the microstructure analysis, PbTe dendrite was horizontally growth to the [112] and [110] direction with (110) plane, and vertically growth to the [111] direction with (111) plane. PbTe growth mechanism was mainly influenced by mass transport and surface energy. When Se was added, ternary PbTexSe1-x was fabricated. PbTeSe showed FCC single crystal structure and dendritic shape. In electrical properties, carrier concentration was increased as Se contents were increased, but carrier mobility was decreased.

      • 중탄소 고실리콘강의 흑연화 거동 및 흑연화 속도 영향 인자 분석

        배상우 경북대학교 대학원 2020 국내석사

        RANK : 247631

        Various heat treatments have been carried out to improve processability and ductility of carbon steel Among them, spheroidization heat treatment for softening of carbon steel has been studied. However, the graphitization heat treatment that forms graphite excellent in lubrication and softening effects, unlike the spheroidization heat treatment, has not been studied much. Graphitization heat treatment is because it takes a long time compared to the spheroidization heat treatment, and various methods for improving this have been studied. Therefore, this study analyzed the graphitization and softening behavior of heat treatment using 0.55C-2.3Si medium carbon high silicon steel containing a large amount of Si, a graphitization promoting element. Also, the graphitization facilitating factor and the degree of acceleration were analyzed by controlling variables such as heat treatment temperature, alloying element and initial microstructure for graphitization facilitating element. In the case of alloying elements, the effects on the graphitization rate through the addition of B and Ti, known as graphitization accelerators, were investigated. To this end, analyze the size, fraction, density, etc. of graphite formed by heat-treating a rolled material to which 10 ppm of B and 140 ppm of Ti were added at 700 ℃ and 750 ℃, respectively, and compare it with steel without B and Ti. It was. In addition, by examining the hardness change with the heat treatment time, the effects of B and Ti addition on the graphitization rate and the softening of the material were investigated in detail.

      • 사할린영주귀국 시설노인의 생활실태 및 만족도에 대한 연구

        배상우 대구대학교 2006 국내석사

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        The aim of this study is to devise the effective fixing support method about life settlement for the elderly who returned permanently from Sakhalin by studying their life conditions & satisfaction to life facility. For this, it intended to inspect the general properties, life conditions of the elderly who returned permanently, satisfaction to life facility by the types of facilities(old people's home, the sanitarium, the professional sanitarium)object for the elderly who returned permanently from Sakhalin living at the four facilities of the country and to inspect & analyze the staffs' by the types of facility evaluations by the operation factors object for operators & staffs, and it intended to draw the needs & problems of the effective settlement support method according to the facility's dwelling. The inspection results of this study are like following. First, the elderly who returned permanently from Sakhalin as the inspection objects transferred at the young age of the late teens mostly, did heavy labors broken off the family, lived with hope for returning home after Liberation. Also they have received the minimum livelihood support from the government and have difficulties in the settlement lives because of the emotional unrest by the health problems owing to the old age and separation sufferings with the children left in Sakhalin. Second, among five sub spheres at the life facility satisfaction inspection to the permanent returning facilities by spheres, service satisfaction was the highest and it was shown as satisfaction orders of the relations, the circumstances, the equipment environment of the facility, and every day lives. After choosing separation with the family of Sakhalin before returning, the satisfaction at the facility life was inspected by support balance problem to the facility life compared with hope about life after returning together with the problems of economy, emotion and health. Third, in case of old people's home, life facility satisfaction by the types of the facility was shown very low relatively at the every day lives, the relations, the satisfaction of the facility's equipment environment compared with the sanitarium and the professional sanitarium. At the satisfaction of the circumstance, the sanitarium was the highest and it was shown as orders of the professional sanitarium & old people's home. However, the significant differences among three groups were shown and it was inspected that the relations with staffs are almost similar. Fourth, at the facility operation evaluation by factors of the staffs, the sanitarium was shown the highest at the whole factors evaluation and it was evaluated as orders of the professional sanitarium & old people's home. Old people's home was shown low evaluation generally, and factors of law systematic, human, environmental & regional administrating as sub spheres were evaluated low. Because old people's home was shown as the lowest evaluation, it was inspected that the facility's effective operation to eliminate the discriminate factors & the preparation of support policy method are urgent. Based on the inspection results of this study, it's intended to present the life conditions & the problems and the improvement methods of life satisfaction of the elderly entering the facilities who returned permanently from Sakhalin like following. First, as the politic support method for the elderly who returned permanently from Sakhalin, the systematic policy support of the government has to be intensified through special law legislation for settlement support and national leading support system promotion corresponding to the balance of settlement support. Second, as the service support methods for the settlement of the elderly who returned permanently from Sakhalin, it has to be secured systematically to increase meeting times with family, and non public support nets have to be constructed possible for normal society unity & regional using through exchange increasing within the community. Third, as the operation support methods by the types of the facility, facility reinforcement for the construction of the pleasantness, comfortableness & security like home, the operation expenses of the elderly returned permanently from Sakhalin welfare facility's conversion into the business expenses of national support, and the support for the mutual supplement & enlargement for the effective operation by the types of facility have to be intensified.

      • 범주형 기본소득에 관한 비교연구 : 기본소득으로의 전환 탐색

        배상우 중앙대학교 사회복지대학원 2021 국내석사

        RANK : 247631

        본 연구는 4차 산업혁명과 전염병 팬데믹 같은 현실에서 나타나는 문제를 해결하기 위한 방안으로 기본소득을 제안하였다. 본 연구의 목적은 범주형 기본소득을 분석하여 기본소득 전환가능성을 모색하고 기본소득 실현을 위해 어떤 방향으로 나아가야 하는지 제언하는 것이다. 본 연구에서는 분석을 위해 Gilbert-Terrel의 산물분석틀과 김교성 등(2017)의 기본소득 이념형을 분석틀로 사용하였다. 정책에 대한 정보는 각 지방자치단체의 추진계획과 언론 보도자료, 조례를 참조하였다. 비교분석의 대상은 서울시 청년수당, 경기도 청년기본소득, 전라남도 농어민 공익수당, 전라북도 농민 공익수당, 강원도 육아기본수당, 제주도 해녀수당 이었다. 각 정책을 청년, 농민, 기타로 나누어 비교분석 하였으며, 기본소득과 어떤 차이가 있는지 알아보았고, 소결에서 내용을 정리하였다. 분석결과 국내 범주형 기본소득 중 강원도 육아기본수당이 기본소득과 가장 유사하였다. 각 정책들의 공통점은 지방세를 재원으로 하며, 모두 일부 인구집단을 대상으로 하였다. 본 연구는 기본소득 실현을 위해 다음과 같이 제언하였다. 첫째, 유사·중복되는 정책을 정비하고 조세제도를 개편하여 재원의 확보가 필요하다. 둘째, 도입하는 정책의 조건을 완화하여 기본소득의 무조건성의 원칙을 지켜야 한다. 셋째, 정부 지원을 통해 지급 대상과 규모를 전국단위로 확대해야 한다. 넷째, 기본소득의 효과성을 입증하기 위한 다양한 실험이 추진되어야 한다. 다섯째, 활발한 토론을 통해 기본소득에 대해 바로 알리고 기본소득에 대한 공감대도 충분히 형성해야 한다 여섯째, 장기간 준비를 하여 기본소득을 실현할 수 있도록 기본소득 실현 로드맵을 구축 해야한다. 연구를 마치며, 저자는 기본소득이 만병통치약은 아니지만, COVID-19로 산업구조가 빠르게 변화하고 있는 만큼, 이제는 문제 해결의 대안으로 기본소득도 함께 심도 있게 논의되어야 할 때라고 주장하였다. This study proposes basic income as a way to solve problems appearing in the Fourth Industrial Revolution and Pandemics etc. The purpose of this study is to analyze categorical basic income to explore the possibility of converting basic income and suggest in what direction it should move toward realizing basic income. In this study, Gilbert-Terrrell's product analysis framework and Kim Kyo-seong et al. (2017) basic income ideological framework were used as analysis frames. For information on policies, refer to each local government's implementation plans, press releases, and ordinances. The subjects of the comparative analysis were ‘youth grants’ in Seoul, ‘youth basic income’ in Gyeonggi-do, ‘public interest grants for farmers and fishermen’ in Jeollanam-do, ‘public interest grants for farmers’ in Jeollabuk-do, ‘basic childcare grants’ in Gangwon-do, and haenyeo grants in Jeju Island. Each policy was divided into youth, farmers, and others, and the analysis was conducted to see how it differs from basic income, and the details were organized in the summary. According to the analysis, the ‘basic childcare grants’ in Gangwon-do was the most similar to the basic income among the categorical basic income in Korea. The commonality of each policy was local taxes, all of which were targeted at some population groups. To realize basic income, the study suggested that: First, it is necessary to secure financial resources by readjusting similar and overlapping policies and reorganizing the tax system. Second, the principle of unconditional basic income should be observed by mitigating the conditions of the policy introduced. Third, the government should expand the target and size of payments to national units through government support. Fourth, various experiments should be carried out to demonstrate the effectiveness of basic income. Fifth, we should immediately inform the basic income through active discussion and form a consensus on the basic income. Sixth, a roadmap for realizing basic income should be established so that basic income can be realized through long-term preparation. At the end of the study, the author argued that although basic income is not a panacea, as the industrial structure is rapidly changing to COVID-19, it is time for basic income to be discussed in depth as an alternative to solving the problem.

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