Analysis of Relationship Between Spermatozoa Ability and Reactive Oxygen Species in Porcine: I. Sperm Preincubation by Xanthine and Xanthine Oxidase

박춘근,정희태,김종흥,이상찬,양부근,김정익,Park, C.K.,Cheong, H.T.,Kim, J.H.,Lee, S.C.,Yang, B.K.,Kim, C.I. The Korean Society for Reproductive Medicine 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3

The objective of this study was to test the effect of catalase on penetration in vitro by spermatozoa preincubated with xanthine and/or xanthine oxidase. The penetration rates were, significantly (p<0.05) higher in spermatozoa preincubated without (66 and 38%) than with (40 and 15%) catalase for 0 and 30 min. When spermatozoa were preincubated and inseminated in medium with xanthine, the penetration rates were significantly higher (p<0.05) in medium with (68, 70 and 49% for 0, 30 and 60 min) than without (33, 41 and 19% for 0, 30 and 60 min) catalase. However, in oocytes were' inseminated with spermatozoa pre incubated with or without catalase in the presence of xanthine oxidase, no decrease in penetrations rates were observed for up to 60 min of preincubation. In another experiment, the penetration rates were significantly (p<0.00l) higher in medium with (75, 55 and 52%) than without (14, 4 and 8%) catalase when oocytes were inseminated with spermatozoa preincubated for 0, 30 and 60 min in the presence of xanthine plus xanthine oxidase. On the other hand, The rate of polyspermy in oocytes penetrated in medium without catalase in the presence of xanthine or xanthine plus xanthine oxidase decreased with time of spermatozoa preincubation. However, no differences were observed in polyspermy rates in the medium with xanthine oxidase alone despite presence of catalase. These results indicate the advantages of spermatozoa pre incubated with xanthine plus xanthine oxidase in the presence of catalase to increase penetration potential and with suppressed polyspermy in porcine. 본 연구는 xanthine과 xanthine oxidase가 첨가된 배양액내에서 전배양된 돼지동결정액의 수정능력에 있어서 catalase의 영향을 검토하였다. 체외수정을 위한 기본 배양액내에서 0 또는 30분간 전배양한 정자는 catalase를 첨가 (40 및 15%)한 경우 보다는 무첨가 (66 및 38%)시 유의적으로 높은 정자침입율을 나타냈지만 (p<0.05), 배양액내에 xanthine을 첨가해 정자를 0, 30 및 60분간 전배양했을 때에는 catalase 무첨가 (33, 41 및 19%) 보다는 첨가시 (68, 70 및 49%) 유의적으로 높은 정자침입율이 인정되었다 (p<0.05). 그러나 xanthine oxidase를 첨가하여 정자의 전배양을 행하지 않은 경우는 catalase의 첨가 (13%) 보다는 무첨가 (51%)시 유의적으로 높은 정자침입율을 나타냈지만 (p<0.01), 전배양(30 및 60분)후에는 catalase의 존재유무와 정자전배양시간에 관계없이 매우 낮은 정자침입율 $(10\sim21%)$을 나타냈다. xanthine과 xanthine oxidase를 동시에 첨가하여 0, 30 및 60분간 정자를 전배양한 경우 catalase의 무첨가 (14, 4 및 8%)보다 첨가 (75, 55 및 52%)시 유의적으로 높은 정자침입율을 나타냈다 (p<0.001). 한편, 다정자침입율은 xanthine, xanthine+xanthine oxidase의 첨가시 정자전배양기간이 길어짐에 따라 감소하였으며, catalase의 첨가보다는 무첨가시 낮은 다정자침입율을 나타냈다. 본 연구의 결과로부터 xanthine과 xanthine oxidase를 동시에 첨가시 catalase의 존재는 정자의 전배양후에도 다정자침입을 억제하면서 수정능력의 유지를 위해 매우 효과적인 것으로 추측되었다.

공명형 다공판 시스템의 흡음특성 해석

김재철(J. C. Kim),이동훈(D. H. Lee),박춘근(C. K. Park) 한국정밀공학회 2014 한국정밀공학회 학술발표대회 논문집 Vol.2014 No.10월

Catalase와 $\beta$-Mercaptoethanol이 돼지 태아섬유아세포 Clonal Lines의 배양에 미치는 영향

권대진,박선영,박춘근,양부근,김정익,정희태,Kwon D. J.,Park S. Y.,Park C. K.,Yang B. K.,Kim C. I.,Cheong H. T. 한국수정란이식학회 2004 한국동물생명공학회지 Vol.19 No.3

본 연구에서는 clonal cell lines을 효율적으로 확립할 수 있는 방법을 제시하기 위하여 배양액 내에 catalase와 $\beta$ME 첨가가 clonal cell line 확립 효율에 미치는 영향을 검토하였다. 임신 50일령의 암퇘지에서 얻은 태아섬유아세포를 2회 passage한 후 동결 보관하였다가 실험에 이용하였다. 단일세포를 catalase나 $\beta$ME가 첨가된 배양액이 들어 있는 96-well dish로 옮겨 배양하였다. 단층이 형성된 세포는 4-well dish로 옮겨주어 배양하였으며, 이후 2회 이상 passage가 가능한 세포를 clonal line이 확립된 세포로 판정하였다. 실험 1에서 clonal line 확립효율에 미치는 catalase와 $\beta$ME의 효과를 검토하기 위하여 세포를 100ng/$m\ell$) catalase와 100nM $\beta$ME가 첨가된 DMEM액 내에서 배양하여 clonal line 확립효율을 검토하였다. $\beta$ME 첨가 시 clonal line 확립효율이 8.3%로 대조구의 3.2%에 비해 유의적으로 높게 나타났다.(P<.0.05). 그러나 catalase 첨가 시(3.6%)에는 대조구와 차이가 없었다. 실험 2에서 catalase 농도(0, 10, 100, 1,000ng/$m\ell$)에 따른 clonal line 확립효율을 검토하였다. 대조구에 비하여 모든 처리구에서 clonal line 확립 효율에 대한 첨가효과가 없었다(0-2.6%). 실험 3에서 $\beta$ME 농도(0, 10, 100, 1,000 nM)에 따른 clonal line 확립효율을 검토하였다. 100 nM의 $\beta$ME 첨가 시 clonal line 확립 효율이 9.4%로 대조구 및 타 처리구(0-l.6%)보다 유의적으로 높게 나타났다(P<0.05). 본 연구 결과 배양액 내 catalase 첨가는 확립효율에 영향을 미치지 않지만, 100nM의 $\beta$ME 첨가로 clonal cell line의 확립효율을 향상시킬 수 있는 것으로 나타났다. This study was performed to examine the effects of catalase and $\beta$-mercaptoethanol ($\beta$ME) on the establishment of clonal lines from porcine fetal fibroblast cells. Fibroblasts derived from a pig fetus (Day 50) were passaged two times before use. A single cell was seeded in 96-well plates and cultured in medium supplemented with or without catalase or $\beta$ ME. Cell colonies were passaged two times into 4-well dish. Cell lines with proliferating potential were classified as an established clonal cell line. In experience 1, the establishment efficiencies were examined by addition of catalase (100ng/$m\ell$) or $\beta$ME (100 uM) in culture medium. The establishment efficiency of $\beta$ME-added group (8.3%) was significantly higher than that of control group (3.2%, P<0.05). However, catalase did not have a positive efffct on the establishment efficiency. In experience 2, the establishment efficiencies were examined by addition of different concentrations of catalase (0-1,000 ng/$m\ell$) in culture medium. However, establishment efficiencies were not different among the different concentrations of catalase (0-2.6%). In experience 3. the establishment efficiencies were examined by addition of different concentrations of $\beta$ME(0-1,000 uM) in culture medium. The establishment efficiency was significantly higher in 100 uM $\beta$ME-added group (9.4%) compare to others (0-1.6%). The result of present study shows that the establishment efficiency of clonal cell lines can be enhanced by the culture in media supplemented with 100uM $\beta$ME. However, catalase did not have a positive effect on the establishment efficiency.

불용성 폴리머를 탄소섬유 보강 Polymer - MDF 시멘트 복합재료의 기계적 특성에 미치는 영향

김태진(T . J . Kim),박춘근(C . K . Park) 한국복합재료학회 1999 Composites research Vol.12 No.3

N/A

돼지 정자의 체외보존에 있어 Pyruvate, Taurine 및 Melatonin의 항산화효과와 평가방법의 상관관계 분석

장현용 ( H. Y. Jang ),박춘근 ( C. K Park ),정희태 ( H. T. Cheong ),김종택 ( J. T. Kim ),이학교 ( H. K. Lee ),임석기 ( S. K. Im ),양부근 ( B. K. Yang ) 한국동물자원과학회 2007 한국축산학회지 Vol.49 No.6

인접 분절 퇴행성 변화에 대한 단독 케이지의 생체역학적 효과

김영현(Y. H. Kim),이성재(S. J. Lee),최경철(K.C. Choi),류경식(K.S. Ryu),박춘근(C.K. Park) 한국정밀공학회 2012 한국정밀공학회 학술발표대회 논문집 Vol.2012 No.5월

전기점화 부실식 기관을 이용한 연소개선에 관한 연구

윤수한(S.H.Yoon),이중순(J.S.Lee),김현지(H.J.Kim),박춘근(C.G.Park),하종률(J.Y.Ha) 한국자동차공학회 1995 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1995 No.11_2

This research is about lean burn which can be satisfied with the limitation of both exhaust gas and fuel consumption. But as the mixing ratio is lean, combustion state becomes unstable, the ignitability becomes bad, and the mean effective pressure decrease. To solve the problems is one of the most important things. The aim of this study is to extend the lean limit and to make rapid combustion for the stable combustion, the improvement of fuel consumption ratio and the decrease of hamful gas. <br/> It is possible to extend the lean inflammability limit in the IDI engine. We can greatly<br/> improve the ignitability in the lean field by providing the sub-chamber with methanol.<br/> This study shows that we can design and produce the sub-chamber type constant volume combustion chamber. We can use methanol as the fuel to be injected into the chamber with the high pressure of 8∼20MPa.<br/> Like this, we define the combustion process, extend the lean inflammability limit therefore we can give you some useful materials for the developments and improvment of lean burn engine.<br/>

자갈도상의 흡음특성에 관한 연구

양윤상(Y.S. Yang),이동훈(D.H. Lee),박춘근(C.K. Park),배영인(Y.I. Bae),전진오(J.J. Oh),이동훈(D.H. Lee),지별임(B.I. Ji) 한국철도학회 2016 한국철도학회 학술발표대회논문집 Vol.2016 No.10

소 수정란의 세포주기 조절에 의한 핵이식에 관한 연구

정희태(H . T . Cheong),임석기(S . K . Im),이준희(J . H . Lee),박춘근(C . K . Park),양부근(B . K . Yang),김정익(C . I . Kim) 한국축산학회 1996 한국축산학회지 Vol.38 No.6

This study was conducted to examine the possibility of cell cycle synchronized nuclear transfer and the effect of the early cell cycle stage of donor embryo on the development of nuclear transplant bovine embryos. Donor embryos were treated with nocodazole, then monitored their nuclear phase and cleavage after release from the nocodazole treatment. Blastomeres cleaved within 3 hr and noncleaved ones were transferred into enucleated oocytes. Some nuclear transplant embryos were fixed to investigate their chromatin structures. Comparatively high proportions(56.0-58.6%) of blastomeres arrested at mitotic phase(M-phase) were obtained following treatments with 0.5∼1.0㎍/㎖ of nocodazole for 16 hr. Cleavage rates of blastomeres within 3 hr after release from nocodazole treatment were average 64%(188/294). After nuclear transfer, 13%(11/85) of embryos were developed to blastocysts following transplantation of blastomeres within 1.5 hr post cleavage(hpc), but 7%(5/74) and 5%(3/63) were developed to blastocysts following transplantation of blastomeres at 3 hpc and noncleaved ones. About 40% of 1.5 hpc nuclear transplant embryos have a single chromatin clump, whereas only 25.0% of 3.0 hpc nuclear transplant eanbryos have a single chromatin clump and the rest have 2 or multi chromatin clumps. From the result of this study, it was suggested that the developmental potential of nuclear transfer bovine embryos may be enhanced with transfer of early cell cycle stage blastomeres immediately after cleavage. It was also suggested that the cell cycle synchronization of bovine embryonic cells at GI phase is very difficult.

뇌출혈의 뇌정위적 제거술

최창락,안명수,이상철,박춘근 대한신경외과학회 1982 Journal of Korean neurosurgical society Vol.11 No.1

1980년 3월부터 6월까지 본원에서는 5예의 고혈압성 뇌출혈 환자에서 래원 즉시 촬영한 두개내 computerized axial tomogram을 이용하여 뇌출혈량과 부위를 계측한 다음 Trasyrol®100 Unit를 2~3일간, 50만 Unit를 5일간 정주하고 steroids, mannitol 또는 glycerol을 정주한 후 Tod-Well stereotaxic apparatus를 이용하여 국소 마취하에 뇌혈종을 제거하고 혈종 부위에 drain를 막아 계속적 배출을 시도하여 2~3주 후 CT scan을 시행하여 혈종이 제거된 것을 확인하면서 치료하였다. 이상과 같은 결과는 뇌정위적 방법으로 국소 마취하에 전신상태가 전신 마취에 견디기 어려운 예나 경제적으로 저렴한 비용으로 실시할 수 있어 좋은 방법으로 사려되어 보고하는 바이다. In 5 cases of intracerebral hematoma due to hypertensive hemorrhage or postcontused hemorrhage, stereotaxic evacuation of the hematoma in the brain was performed. CT scan was done for definite diagnosis, localization and coordinating target. In order to make possible the evacuation of coagulated hematomas through a simple burr hole, a specially designed instrument (Tod-Well stereotaxic guide) and infusion of proteolytic enzyme (Trasyrol^(R)) were used. While we have treated these patients with antiedematous agent, it was possible to wait for 4-5 days to attempt in expectation of clot hematoma. Timing is of paramount important for success with this technique. Therefore CT scan must be done for the detection of characteristic of the blood clot state. This method is simple and it can be done under the local anesthesia. This stereotaxic evacuation method does not decrease high mortality rate of intracerebral hematoma, but morbidity of the patient was improved and this method offers a less bloody operation for patients with slightly neurological deficits presenting lesions in deep brain structures.