Bacillus firmus Cyclodextrin Glycosyltransferase의 정제 및 특성

손천배(Cheon-Bae Sohn),김성애(Seong-Ai Kim),박영아(Young-A Park),김명희(Myung-Hee Kim),문숙경(Sook-Kyung Moon),장순애(Sun-Ae Jang),이명선(Myung-Sun Lee) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.2

Bacillus firmus가 생산하는 cyclodextrin glycosyltran-sferase(CGTase)를 ammonium sulfate 침전, DEAE-Sephadex A-50 및 Sephdex G-100 column chromatography로 분리, 정제하였다. 이 방법으로 수율은 12%, 정제도는 49배인 SDS-PAGE 상에서 단일 band의 효소 단백질을 얻을 수 있었다. 정제된 CGTase는 Phast system의 SDS-polyacrylamide gel 전기영동에 의하여 분자량은 80,000dalton, isoelectric focusing으로 등전점은 9.6으로 추정되는 단백질이었다. 이 효소의 최적 활성 pH와 온도는 8.0,65℃이였으며, pH와 열안정성은 pH 5.5~9.0,50℃이였다. Soluble starch를 기질로 하여 24시간 효소반응한 액의 α- : β- : γ-cyclodextrin의 생성비율은 0.01 : 2.90 : 1.00으로 β- 및 γ-cyclodextrin을 주로 생산하였다. The cyclodextrin glycosyltransferase(EC 3.2.1.19) from Bacillus firmus was purified by precipitating with ammonium sulfate followed by, DEAE-Sephadex A-50 column chromatography and Sephadex G-100 column chromatography. In this way, we were able to obtain the single band protein on SDS-PAGE with a yield of 12%, whose purity was 49 fold. The purified CGTase was identified as a protein having molecular weight of approximately 80,000 dalton and isoelectric point of 9.6. The optimum pH and temperature for the enzyme activity were 8.0 and 65℃, respectively. The enzyme was stable at between pH 5.5 and 9.0 and up to 50℃. After 24hr of enzyme reaction using soluble starch as substrate, the ratio of α-, β- and γ-cyclodextrin production was 0.01 : 2.90 : 1.00, respectively. And this CGTase pro-duced mainly β- and γ-cyclodextrin.

온라인 무료체험 서비스 만족과 유료 회원 가입 의도간의 구조적 관계에 관한 연구

박영아(Young A Park) 한국인터넷전자상거래학회 2021 인터넷전자상거래연구 Vol.21 No.5

Due to COVID-19, social distancing, movement restrictions, and working from home have increased the time spent indoors, resulting in a sharp increase in the use of online and mobile services. Despite the increase in online and mobile shopping, there is a lack of interest in free trials, a marketing strategy that traditionally has a significant impact on consumer behavior. Online free service, which is a type of information technology service, allows users to experience the service for free, leading to initial use of the service and subsequent purchase. The purpose of this study is to examine the factors influencing the satisfaction of online free experience service and to find out whether subjective norms play a role as a moderating variable between the intention to sign up for online paid experiences. This survey was conducted from May 3 to May 10, 2021, targeting undergraduate and graduate students of universities located in Gyeongsangbuk-do. The URL of the written question was provided through the Google survey function and the questionnaire was filled out, and a total of 294 questionnaires were used for the final analysis. The results of this study are as follows. First, perceived value, perceived risk, and perceived ease were found to have a significant effect on satisfaction with online free experience, supporting the results of previous studies. On the other hand, subjective norms do not play a role as a moderating variable between satisfaction with online free experience and intention to sign up for online paid membership, so follow-up studies are needed. This study is meaningful in that it provided theoretical and practical implications necessary for the introduction and expansion of online paid members through online free trial services as non-face-to-face purchases rapidly increased due to the spread of COVID-19.

성별이 온라인 의사결정 스타일에 미치는 효과에 관한 연구 : 항공티켓 구매자를 중심으로 A Case of Airline Ticket Shoppers

박영아 ( Young A Park ),이충기 ( Choong Ki Lee ) 대한관광경영학회 2008 觀光硏究 Vol.22 No.4

정보기술 혁신수용정도에 따른 “철도 여행안내센터” 이용자 만족도 측정에 관한 연구

박영아(Young A Park) 한국호텔외식관광경영학회 2013 호텔경영학연구 Vol.22 No.4

The purposes of this study were threefold: (1) to propose a model with information quality, system quality, and service quality as the key factors to influence users` satisfaction with travel information centers in railroad stations, (2) to examine the relationship between users` satisfaction and their intention to revisit travel information centers; and (3) to examine the moderating effect of the acceptance level of information technology on the relationships between three independent factors and users` satisfaction with travel information centers. The 330 questionnaires were distributed to visitors of Dong Daegu railroad station. The findings indicated that first, the information quality and service quality were significantly related to the users` satisfaction with travel information center while system quality did not. Second, the users` satisfaction positively affected their intention to revisit travel information centers. Finally, acceptance level of information technology played a moderating role in the relationship between the service quality and the users` satisfaction with travel information centers. Overall, the findings suggest that employees need to continuously provide updated information and maintain service quality to capture visitors` new needs since macro-environmental change in IT environments might require changes to travel information centers. Limitations of the studies and agenda for future research were also discussed.

박물관 정보기술 사용의도에 미치는 영향요인에 관한 연구

박영아(Park, Young-A) 대한경영학회 2011 大韓經營學會誌 Vol.24 No.5

공간론적 관점에서 관광목적지의 해양관광개발계획에 대한 분석 : 부산시를 중심으로

박영아(Park Young-A),양위주(Yhang Wii-Joo) 동북아시아문화학회 2008 동북아 문화연구 Vol.1 No.17

This study is to suggest the political alternatives through the analyses of marine tourism master plans out of the central and local government (Busan) plans, which were based on the literature reviews about the two theories of tourist destination- hierarchial place vs organization-, reflecting current situation under marine tourism development nation wide. Then, to accomplish the purpose is to find out future vision of Busan by analyzing the direction and content of tourism-related plans established since 1990s. The results found that visions from most plans suggest the image as a marine city based on marine resources, but most destination not be really developed as plans suggested. Second, the central and local government related to tourism development of Busan repeatedly produced similar but different named outputs depending on repeatability and periodicity of plans after the mid of 1990s. In conclusion, urban space of Busan as a tourist destination should be reevaluated from the viewpoint of the hierarchial place theory. However, physical plans that were oriented to hierarchy and lack of definite contents should accept the lessons from the organizational theory, which a tourist destination is dependent on a life cycle model. Busan must be prepared to the fitness of survival strategy from changing and dynamic tourism markets inside and outside.

메가이벤트에 대한 지역민들의 관람동기, 태도 및 참여의사 간 영향관계 검증에 관한 연구

박영아(Park, Young-A) 대한경영학회 2013 大韓經營學會誌 Vol.26 No.10

호텔 웹사이트 품질, 웹사이트 만족, 호텔 방문의도 간 구조적 영향관계에 관한 연구

김순경(Soon Kyung Kim),박영아(Young A Park),현용호(Yong Ho Hyun) 한양대학교 관광연구소 2013 觀光硏究論叢 Vol.25 No.1

본 연구의 목적은 호텔 웹사이트 품질을 선행변수로 하여 은행서비스 제1호(통권 34) 24 선행변수로 하여 웹사이트 만족과 호텔재방문의도 간의 구조적 영향관계를 검증하는 것 이다. 본 연구를 위해 설문조사는 대구 인터불고 호텔 웹사이트를 방문한 고객을 대상으로 설문을 실시하였다. 총 700부가 온라인을 통해 설문이 배포하여 678명의 응답자가 온 라인 설문에 응하였으며 불성실하게 응답을 한 설문을 제외한 323부가 최종 분석에 이용 되었다. 본 연구 결과는 다음과 같다. 첫째, 정보품질과 서비스 품질이 웹사이트 만족에 정(+)의 영향을 준 것으로 나타난 반면 시스템 품질은 영향을 주지 않은 것으로 밝혀졌 다. 유의미한 결과를 보여준 경로 중, 정보품질이 웹사이트 만족도에 상대적으로 더 많은 영향을 준 것으로 나타났다. 둘째, 웹사이트 만족이 호텔재방문의도에 정(+)의 영향을 준 것으로 밝혀졌다. 호텔 경영자 및 관리자들을 위한 이론적?실무적 시사점이 제시되었고 마지막으로 한계점이 논의되었다. The purpose of this study is to examine the structural relationships between the web site quality of the deluxe hotels in Daegu, web-usage satisfaction and a revisit intention to a hotel. The web questionnaires were distributed to customers who visited the web site of the hotel, ``Hotel Inter-Burgo Daegu``. The self-administerd survey was undertaken against 700 respondents. A total of 678 responses were collected. Excluding missing data, 323 usable data were used for analysis. Results from this study are as follows: First, it was found that information quality and service quality positively influenced the web-usage satisfaction, whereas system quality did not. Information quality had the most effect on hotel web-usage satisfaction; second, it was revealed that the web-usage satisfaction positively affected the revisit intention to the hotel. The theoretical and practical implications are discussed. The limitations are also mentioned.

호텔 내부고객의 직무만족과 고객지향성이 외부고객이 지각한 서비스품질, 만족도, 충성도에 미치는 영향

황대욱 ( Dae Uk Hwang ),박영아 ( Young A Park ) 대한관광경영학회 2014 觀光硏究 Vol.29 No.5

본 연구의 목적은 호텔을 방문한 고객들을 대상으로 호텔 내부고객의 직무만족, 고객지향성이 호텔 외부고객의 서비스품질, 고객만족, 충성도에 미치는 영향관계를 파악하고자 하였다. 본 연구를 위해 설문조사는 경주지역에 위치한 특급 호텔을 대상으로 2013년 7월 1일부터 8월 10일까지 편의표본추출을 통해 실시되었으며, 종사원과 고객의 1:1 서비스 상황을 확인 후 종사원과 해당 종사원에게 서비스를 받은 고객을 대상으로 동시에 설문조사를 실시하였다. 총 종사원을 대상으로 한 설문지 83부와 해당 종사원을 통하여 서비스를 받은 고객을 대상으로 한 220부가 본 조사 분석에 사용되었다. 본 연구 결과는 다음과 같다. 첫째, 직무만족이 고객배려, 고객 중심 및 신뢰성 모두에 유의한 영향을 주는 것으로 밝혀졌다. 둘째, 직무만족은 서비스품질 요인인 물적·시스템품질, 인적 품질에 유의한 영향을 주지 않는 것으로 나타났다. 셋째, 고객지향성 요인 중 고객중심적 사고 요인은 물적·시스템품질에, 고객에 대한 신뢰는 인적 품질에 유의한 영향을 주는 것으로 나타났다. 넷째, 서비스품질인 물적ㆍ시스템품질 요인과 인적 품질이 고객만족에 모두 유의한 영향을 주는 것으로 밝혀졌고,마지막으로, 고객만족이 충성도에 정(+)의 영향을 미치는 것으로 나타났다. 호텔 경영자 및 실무자들을 위한 이론적.실무적 시사점이 제시되었고 마지막으로 한계점이 논의되었다. The objective of this study was to determine the effect of job satisfaction and the customer-oriented attitudes of internal hotel customers on service quality, satisfaction, and loyalty of external hotel customers who visited a five-star hotel. The survey was conducted with employees of a five-star hotel and hotel customers from July 1, 2013 to August 10, 2013. The data wer collected from 238 hotel employees and 83 hotel customers. A total of 220 questionnaires were answered and analyzed for this study. The study results are as follows. First, the job satisfaction of hotel employees had a positive (+) effect on three factors related to customer-oriented attitude: consideration, customer-centered thinking, and trust towards customers. Second, the job satisfaction of the hotel employees did not affect all the factors related to service quality. Third, the customer-centered thinking of a customer-oriented attitude had a significant effect on human, physical, and system qualities while trust of customers had a significant effect only on human quality. Fourth, service quality had a positive (+) effect on customer satisfaction. Finally, the relationship between customer satisfaction and loyalty showed a positive (+) effect on one another. Finally, the overall findings, limitations of the studies, and agenda for future research were discussed.

술기 수행에서 간호대학생의 동료피드백 유형 및 동료피드백에 대한 정서반응, 피드백의 질, 학습자기효능감

박영아(Park, Young A),김은정(Kim, Eun Jung) 한국간호교육학회 2019 한국간호교육학회지 Vol.25 No.2

Purpose: This study was conducted to identify types of peer feedback in nursing skills performance training and to investigate emotional response, perceived quality of feedback and self-efficacy for learning from peer feedback. Methods: A total of 110 second-year nursing students attending fundamentals of nursing classes at a university participated in 2017. Participants received peer feedback from one selected colleague who observed their skills performance, and completed a questionnaire. Contents of peer feedback video recordings were transcribed and classified into seven types of feedback. Data were analyzed using an independent t-test, ANOVA and Pearson correlation. Results: Participants mostly received feedback of ‘correcting’ and ‘providing information.’ Positive emotional responses from peer feedback were above medium, and negative emotional responses were low. The perceived quality of peer feedback was moderate and self-efficacy for learning was high. Quality of feedback correlated with negative emotional response (r=-.24, p=.014), and self-efficacy for learning (r=.35, p<.001). Self-efficacy for learning correlated with intimacy with peers (r=.24, p=.011) and positive emotional response (r=.21, p=.028). Conclusion: The results suggest that peer feedback in skill training could be used in terms of emotional response and self-efficacy for learning.