Cross Hole Type 비 연삭 밀링 인서트의 가공특성에 관한 연구

김택수,이상민,박휘근,채승수,이충석,손일복,김학규,이종찬 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

This paper reports some experimental results on the machining performance of non-ground cross hole type milling inserts. Six kinds of milling inserts were manufactured without using grinding process. Machining experiments were carried out to compare the machining performance of non-ground inserts with that of ground ones. The experimental results indicate that the cutting forces and surface roughnesses of machined surface of both ground and non-ground inserts are comparable.

Vinblastine이 백서 배양 Type-1 별아교세포에 미치는 세포독성에 관한 연구

하상호,박승택,문연자,김종영,김정중,정연태 圓光大學校 醫科學硏究所 1994 圓光醫科學 Vol.10 No.1-2

It has been reported that vinblastine, anticancer drug, is a neuroteratogen inducing various neural malformations such as microcephaly and neural tube defect(NTD), but the study on the toxicity of neuroglial cells, especially type-1 astrocytes using culture system is not well established. In order to evaluate the cytotoxicity of vinblastine on cultured type-1 astrocytes of neonatal rat brain in vitro. MTT assay and also light and electron microscopic studies were carried out. The results were as follows: 1. MTT_90 and MTT_50 in cultured type-1 astrocytes were 1×10 exp (-1) μM and 1×10 exp (2)μM of vinblastine, respectively. 2. Vinblastine was highly toxic in cultured type-1 astrocytes(MTT_50 ≤ 100 μM). 3. In a light microscopy, cultured type-1 astrocytes showed decrease of cells in number, cytoplasmic perforation, vacuolization and cytoplasmic granulation after cultured type-1 astrocytes were treated with 1×10 exp (2)μM of vinblastine for 24 hours. Cultured type-1 astrocytes damaged by vinblastine showed degenerative changes of cells morphologically. 4. In an electron microscopy, increment of glial fibrillary acidic protein(GFAP), increased free-ribosomes, cisternal dilatation of rough endoplasmic reticulum (rER). few dense bodies and vacuoles were shown in cultured type-1 astrocytes treated with 1×10 exp (2)μM of vinblastine for 24 hours. The results indicate that vinblastine has markedly cytotoxic effect on the type-1 astrocytes of neonatal rat brain in vitro study.

신경세포성장인자로서 척수운동신경세포의 손상에 미치는 한약재의 약류별 효능 및 기전에 관한 연구

鄭遇悅,朴承澤,成彊慶,李星根 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

산소자유기가 흰쥐의 배양 운동신경세포에 미치는 세포독성에 대한 기전을 규명하기 위하여 여러 농도의 산소자유기를 배양 신경세포에 처리한 후 산소자유기의 신경독성 효과를 분석하였으며 또한 산소자유기에 의하여 유발된 신경독성에 대한 한약재중 보혈약류와 보기약류의 대표적인 처방인 사군자탕과 사물탕의 신경세포독성의 방어효과를 MTT assay법에 의하여 조사하여 다음과 같은 결론을 얻었다. Xanthine oxidase(XO)와 hypoxanthine(HX)은 처리한 농도에 비례하여 배양 신경세포의 생존율을 현저하게 감소시켰으며, 또한 사물탕과 사군자탕이 XO/HX의 독성효과를 유의있게 방어하였다. 이상의 결과로부터 산소자유기는 배양 소의 희소돌기아교세포에 독성을 나타냈으며 사군자탕과 사물탕등의 한약추출물이 산소자유기의 독성을 방어하는데 효과적인 것으로 나타났다. In order to elucidate the neuroprotective effect of Sagunjatang and Samultang on oxidant-induced neurotoxicity in cultured rat spinal motor neurons, the colorimetric assay such as MTT assay was performed and light microscopic study was also carried out after cultured spinal motor neurons from neonatal rat were treated with the medium containing various concentrations of xanthine oxidase(XO) and hypoxanthine(HX) for 3 hours. In addition, protective effect of herb extracts on the neurotoxicity induced by oxygen radicals was examined in these cultures. The results were as follows : Cell viability of cultured rat spinal motor neurons which were exposed to various concentrations of 30mU/ml XO and 0.1mM HX for 3 hours, was decreased in a dose-dependent manner. MTT50 value was 30mU/ml XO and 0.1mM HX after motor neurons were incubated with various concentrations of XO/HX for 3 hours. Cultured spinal motor neurons showed degenerative changes such as the decrease of cell number and loss of cell processes when neurons were cultured in the medium containing 30mU/ml X0 and 0.1mM HX for 3 hours. Oxygen radicals were toxic on cultured rat spinal motor neurons by the decrease of cell viability. Herb extracts, Sagunjatang and Samultang prevented the oxidant-induced neurotoxicity significantly after spinal motor neurons were preincubated with herb extractions for 2 hours before exposure of oxygen radicals. These results suggest that oxygen radicals have neurotoxic effect on cultured spinal motor neurons derived from neonatal rat, and selective herb extracts such as Sagunjatang and Samultang are very effective in protecting the neurotoxicity induced by oxygen radicals.

생쥐의 卵子와 初期胚子에 對한 Clomiphene Citrate의 細胞毒性에 關한 硏究

金惠敬,潘勝一,金洋一,文蓮子,朴承澤,鄭然泰 圓光大學校 醫科學硏究所 1993 圓光醫科學 Vol.9 No.1-2

In order to elucidate the cytotoxicity of clomiphene citrate, the rate of in vitro fertilization(IVF) of ova and the developmental rate of early embroys in A-strain mouse were examined. The rate of ova cleavaging to 2-cell stage by IVF was remarkably decreased(12.6%) at the concentration of 10μg/㎖ of clomiphene than that of the control(65.2%) when the ova fertilized in vitro were cultured for 24 hours in the untreated medium after treatment ova with clomiphene for 5 hrs. But there was no ovum cleavaging to 2-cell stage at 50μg/㎖ clomiphene except only a few 2-polar bodied ova. The developmental rate of mouse early embryos was decreased dose-dependently. Especially at 25μg/㎖ of clomiphene, the number of embryos cleavaging to blastocyst from 2-cell stage(41.4%) was decreased remarkably compared with that of the control(87.2%). And only a few embryos were developed to 8-cell stage at clomiphene concentration of 50μg/㎖ after 2-cell embryos were incubated for 72 hours in clomiphene-treated medium. Morphological changes such as fragmentation and fusion of blastomeres, cytolysis and developmental retardation were increased with dose-dependently. These results suggest that clomiphene has cytotoxic effect by the decrease of the rate of IVF, the developmental rate and the degenerative changes of murine ova and early embryos.

흰쥐의 감각신경절세포에 대한 Methylmercuric chloride의 독성효과

鄭榮吉,白承和,韓斗錫,柳道坤,朴承澤 한국전통의학연구소 1998 한국전통의학지 Vol.8 No.1

흰쥐의 배양 척수감각신경절세포에 대한 methylmercuric chloride(MMC)의 세포독성을 조사하기 위하여 여러 농도의 MMC가 포함된 배앙액에서 24시간 동안 배양한 후 MTI assay법에 의하여 세포의 생존율을 조사하였다. MMC는 농도에 비례하여 세포의 생존율을 감소시켰다. 또한 여러 농도의 MMC가 포함된 배앙액에서 흰쥐의 척수 감각신경절(dorsal root ganglion, DRG) 세포를 24시간 동안 처리한 결과 MTI_(50) 값이 25μM MMC에서 나타났다. MMC는 Borenfreund등(1988)의 독성판정기준에 의하여 흰쥐의 배앙 DRG 세포에 고독성인 것으로 나타났다. 이상의 결과는 MMC가 흰쥐의 배양 척수감각신경절세포에 강한 신경독성 효과가 있음을 제시하였으며 또한 배양신경세포는 약제의 효과를 검색하는데 효과적이었다. To examine the cytotoxic effect of methyl mercuric chloride(MMC) on the cultured rat spinal dorsal root ganglion(ORG) neurons, cell viability was measured by MTT assay after rat spinal ORG neurons were incubated with media containing various concentrations of MMC for 24 hours The results were as follows ; 1. MMC decresed cell viability of rat spinal ORG neurons in a dose-dependent manner. 2. MIT_(950) value was a 25uM MMC after spinal ORG neurons were cultured for 24 hours at various concentrations of MMC. 3. MMC was heighly toxic on the cultured ORG neurons by the toxic criteria of Borenfreund et al.(1988). From above the results, it is suggested that methylmercury involves in neurotoxicity by the decrease of cell viability in cultured rat spinal DRG neurons, and cultured neurons are effective in screening the putative agents.

Oxidative Stress에 의해 손상된 대뇌신경세포에 대한 Vitamin E의 효과

조정구,김형민,박승택 한국노화학회 1998 한국노화학회지 Vol.8 No.3

메틸水銀의 心筋毒性에 대한 苦蔘의 影響

송호준,하대호,유교상,박승택,이강창,서부일 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

Objectives : To evaluate the toxic effect of methylmercuric chloride(MMC) and the protective effect of Sophorae Radix(SR) in cultured mouse myocardial cells, cytotoxicity was measured by MTT assay Methods : Cultured myocardial cells were incubated for 72 hours in the media containing 5~40 ㎛ concentrations of MMC. And also, the protective effect of SR was determined by sulforhodamine B(SRB) for protein sythesis and radioactive assay for DNA sythesis in these cultures. Results : Cell viability was significantly decreased in a dose-dependent manner after cultured myocardial cells were exposed to 20 ㎛ MMC for 72 hours. In the neuroprotective effect of SR on MMC-induced cytotoxicity, SR blocked the MMC-induced myotoxocity in these cultures. Conclusion : It suggests that MMC is toxic against cultured mouse myocardial cells and SR is effective in blocking the meurotoxicity induced by MMC.

어류 성장에 있어서 식물 및 동물플랑크톤의 먹이기여도

김백호,김미연,최환석,김정중,최민규,박승택,정연태,高村典子 원광대학교 환경과학연구소 2000 環境科學硏究誌 Vol.9 No.1

익산시 주변 하천 담수조류군집의 다양성에 대하여

최환석,김미연,김백호,최민규,박승택 원광대학교 환경과학연구소 2000 環境科學硏究誌 Vol.9 No.1

ToxIcity of Oxygen Radicals on Cerebral Cotical Neurons of Mouse

Seung Taeck PARK,Jung Mu HER,Jae Min OH,Jung Joong KIM,Mln Kyu CHOI,Yeun Tal CHUNG 대한체질인류학회 2000 대한체질인류학회 학술대회 연제 초록 Vol.43 No.-