돼지의 체외수정

박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.1

The present study was conducted to investigate the optimum conditions of in vitro fertilization by ejaculated boar spermatiozoa capacitated in a defined medium. When fresh ejaculated boar spermatozoa were preincubated for 4h with elevated sperm concentration fertilization rates were markedly increased. The fertilization rates by spermatozoa preincubated at the concentrations of 2, 4, 10, 20 and 40×10^8 cells/ml were 12, 30, 50, 76 and 82%, respectively, However, when spermatozoa preserved for 10-12h at 20℃ were preincubated for 4h with elevated sperm concentration fertilization rates were markedly decreased. The fertilization rates by spermatozoa preincubated at the concentrations of 2, 4, 10, 20 and 40×10^8 cells/ml at preincubation were 91, 77, 68, 44 and 8%, respectively. When oocytes matured in vitro were inseminated with spermatozoa at the concentration 1 × I0^6 cells/ml, rates of fertilization and polyspermy were 97 and 87%, respectively, after culture for 20h without changing medium. When oocytes were inseminated with spermatozoa at the different concentrations of 1, 0.5, 0.25 and 0.1×10^6 cells /ml and the medium was changed 5h after insemination, the fertilization rates were 75, 73, 61 and 30%, respectively. The rates of polyspermic fertilization were 58, 47, 40 and 47%, respectively, when oocytes were inseminated with different concentrations of spermatozoa, and there was no difference in the rates of polyspermy fertilization among groups with changing medium. The present results suggested that both sperm preincubation with high concentration and sperm preservation at 20℃ for 10-12h were effective for capacitation ejaculated boar spermatozoa and that a complete block of polyspermy was not achieved in this in vitro fertilization conditions.

연속일체형 날개-동체 타입 UCAV 형상의 저속 종방향 공력특성에 대한 전산유동해석

박상현(S.H. Park),장경식(K. Chang),심호준(H.J. Shim),신동진(D.J. Sheen),박수형(S.H. Park) 한국전산유체공학회 2016 한국전산유체공학회지 Vol.21 No.3

In the present work, numerical simulations were conducted on the scaled model of the BWB type UCAV in the subsonic region using ANSYS FLUENT V15. The prediction method was validated through comparison with experimental results and the effect of the twisted wing was investigated. To consider the transitional flow phenomenon, γ transition model based on SST model was adopted. The coefficients of lift, drag and pitching moment were compared with experimental results and the pressure distribution and streamlines were investigated. The twisted wing decreases the lift force but increases lift-to-drag ratio through delay of stall and leading edge vortex’s movement to the front, also the non-linearity of the pitching moment is decreased.

디젤-가솔린 혼합연료의 분무 미립화 특성에 관한 연구

박세원(S. Park),이동곤(D. Lee),박수한(S. H. Park),노현구(H.G. Roh),박성욱(S. Park),이창식(C. S. Lee) 한국자동차공학회 2012 한국자동차공학회 부문종합 학술대회 Vol.2012 No.5

분만후 젖소에 있어서 $PGF_2a+PGF_2a+CIDR$ Program 적용에 의한 발정유기시 BCS의 영향

백광수,박성재,박수봉,김현섭,이현준,이왕식,전병순,안병석,김재기,정경용,손준규,Baek K. S.,Park S. J.,Park S. B.,Kim H. S.,Lee H. J.,Lee W. S.,Jeon B. S.,Ahn B. S.,Kim J. G.,Jeong G. Y.,Son J. K. 한국수정란이식학회 2005 한국동물생명공학회지 Vol.20 No.3

본 연구는 젖소에 있어서 분만후 난소 활동의 재개 및 분만후 40일째 BCS가 $PGE_2a+PGE_2a+CIDR$ (PPC) program 적용에 미치는 영향을 구명하기 위하여 2002년부터 2003년까지 2년간에 걸쳐 축산연구소 및 천안 소재 전문 경영체 젖소농 장에서 사육중인 홀스타인 착유우를 대상으로 분만 후 난소 활동의 재개를 조사하기 위한 착유우21두 및 분만후 40일째 BCS가 PPC program 적용에 미치는 영향을 구명하기 위한 착유우 40두를 공시하여 수행한 결과 다음과 같은 성적을 얻었다. 1 분만후 처음으로 유즙중의 progesterone농도가 1.0ng/mL 이상을 나타내는 비율은 10${\~}$30일이 $42.9\%$, 31${\~}$50일이 $19.1\%$였고 분만 후 50일까지 1.0ng/mL 이상을 나타내지 않은 경우가 $38.1\%$였다. 2. 분만후 40일째부터 PPC program을 투입한 결과 1차 및 2차 $PGF_2a$ 처리에 대한 발정반응은 각각 $47.5\%$ 및 $52.4\%$였고, $PGF_2a+PGF_2a$ (PP) program투입에 의해 40두중 30두($75\%$)가 발정 반응을 나타내었다. PP program 투입에 의해 발정 반응을 나타내지 않은 10두에 대한 CIDR 처리의 발정 반응은 $50\%$를 나타내었다. 분만후 40일째에 PPC program 을 적용한 결과 40두중 35두가 발정 반응 ($87.5\%$)을 나타내었다. 3. 1차 및 2차 $PGF_2a$ 처리에 따른 발정 반응은 BCS 2.50 이하의 경우 13두중 8두($61.5\%$), BCS 2.75${\~}$3.50의 경우 27두중 22두($81.5\%$)로 2.75${\~}$3.50의 경우가 2.50 이하의 경우에 비하여 높은 발정반응을 나타내었다. 4. 2차 $PGF_2a$ 처리후 발정 반응을 나타내지 않은 개체에 대하여 CIDR을 처리한 결과, BCS 2.50이하의 경우 5두중 2두에서 발정 반응($40.0\%$)을 나타내었고 BCS 2.75${\~}$3.50의 경우 5 두중 4두에서 발정 반응($80\%$)을 나타내었다. 5. 분만후 40일째부터 PPC program을 적용하여 발정 반응을 검토한 결과 BCS 2.50 이하의 경우 13두중 10두가 발정 반응($76.9\%$)을 나타내었고 BCS 2.75${\~}$3.5의 경우 27두중 26두가 발정 반응($96.3\%$)을 나타내어 2.75${\~}$3.5의 경우가 2.50 이하의 경우에 비하여 높은 발정반응을 나타내었다. This study was carried out to investigate resumption of ovarian cyclicity and effect of BCS on estrous response by treatment of $PGF_2a+PGF_2a+CIDR$ program on day 40 postpartum in lactating dairy cow. First $PGF_2a$ was given on day 40 postpartum, second $PGF_2a$ was given 14 days apart to cows not-responded to 1st $PGF_2a$ and then CIDR was inserted for 7 days after 5 days in cows not-responded to 2nd $PGF_2a$. The $42.9\%$ of the cows showed more than 1 ng/mL milk progesterone concentration within 10 to 30 days postpartum. About $19\%$ of the cows exhibited more than 1 ng/mL milk pro-gesterone concentration between 31 to 50 days postpartum. However $38.1\%$ of the cows have not shown more than 1 ng/mL milk progesterone up to 50 days postpartum. Estrous response to the treatment of 1 st $PGF_2a$ and 2nd $PGF_2a$ was $47.5\%$ and $52.4\%$, respectively. Combination of 1 st $PGF_2a$ and 2nd $PGF_2a$ was $75\%$ and combination of 1st $PGF_2a$+2nd $PGF_2a$+CIDR was $87.5\%$. Estrous response to the treatment of $PGF_2a+PGF_2a$ program was $61.5\%$ in cows with less than 2.50 BCS and $81.5\%$ in cows with 2.75${\~}$3.50 BCS. Estrous response to the treatment of CIDR was $40\%$ in cows with less than 2.50 BCS and $80\%$ in cows with 2.75${\~}$3.50 BCS. Estrous response to the treatment of PPC on day 40 postpartum was $76.9\%$ in cows with less than 2.50 BCS and $96.3\%$ in cows with 2.75${\~}$3.50 BCS.

신개발 세족기 및 세족액의 젖소 적응효과

백광수,김병학,박수봉,박성재,김현섭,이왕식,기광석,전병순,안병석,강석진,서국현,Baek, K.S.,Kim, B.H.,Park, S.B.,Park, S.J.,Kim, H.S.,Lee, W.S.,Ki, K.S.,Jeon, B.S.,Ahn, B.S.,Kang, S.J.,Suh, G.H. 한국축산환경학회 2006 축산시설환경학회지 Vol.12 No.3

본 연구는 젖소에 있어서 세족기 및 신개발 세족액의 적용효과를 구명하기 위하여 2004년 축산연구소 및 전문경영체농장 2개소에서 사육중인 홀스타인 착유우를 대상으로 신개박 세족기 및 세족액을 적용하여 다음과 같은 결과를 얻었다. 1. 유산동의 MIC와 MBC는 E. coli 및 Bacillus에 대해서 공히 0.31%로 나타났다. 신개발 세족액의 경우 E. coli에 대해서는 MBC가 5%로 나타났고 Bacillus에 대해서는 2.50%로 나타났다. 2. E. coli의 경우 5% 및 16% 농도에서 각각 95.69% 및 95.87%의 감소율을 나타내었고 Bacillus의 경우 5% 및 16% 농도에서 각각 99.5% 및 99.89%의 감소율을 나타내었다. 3. 30일동안 사용하였을 때 보행자세지수(locomotion score ; LS) 2, LS3, LS4 및 LS5의 경우 100%, 43.8%, 14.3% 및 0%의 정상회복율을 나타내었고 누적하여 60일동안 사용하였을 때 LS2, LS3, LS4 및 LS5의 경우 100%, 81.3%, 71.4% 및 50.0%의 정상회복율을 나타내었다. 4. 신개발 세족액을 사용하기 전과 후의 발굽장애 발생율에 있어서 사용 전에는 18.8%의 발생율을 보였으나 사용 후에는 1.5%의 발생율을 나타내었다. This study was carried out to investigate the effect of new foot-bath facility and detergent solution (sodium molylbdenate, citrate, potassium nitrate, tataric acid, sodium hypo-cholorite, and zinc sulfate) on claw health in lactating dairy cows. Minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) of copper sulphate were 0.31% for E. coli and Bacillus isolated from cows claw. The MIC and MBC of new detergent for E. coli were 1.25% and 5%, respectively, however their respectively values for Bacillus were noticed 0.63% and 2.5%. Both 5E. coli and Bacillus populations in petri-dishes were significantly reduced (more than 95%) with the application of new detergent solution (5% or 16%). Locomotion score (LS 1-5; very good to severely bad) of lactating cows were significantly improved with in 30 days with the use of new detergent solution in foot bath. The LS2 (n=16), LS3 (n=16), and LS4 (n=7) were shown 100%, 43.8%, and 14.3% recovery rate within 30 days with the use of new detergent solution. However, LS5 (n=2) were not recovered to normal claw health and locomotion score within 30 days of new detergent application. Usage of new detergent solution for 60 days in a foot bath have shown 81.3%, 71.4% and 50.0% recovery rate in cows with LS3, LS4 and LS5, respectively. Abnormal claw incidence was reduced from 18.8% to 1.5% in overall herd (n=80) with the use of new detergent solution (16%) in a foot bath for 90 days. In conclusion, usage of 16% of our detergent solution for 60 days in a foot bath can significantly improve the cow claw health and thus mitigate the negative effects of abnormal claw on productivity of cows and dairy farm income.

롤투롤 연속 코팅 시스템에서의 정밀 장력 제어를 위한 스팬별 최적 장력 설정에 대한 연구

박수성(S. Park),신기현(K. -H. Shin),성진우(J. Seong),이종수(J. Lee),길효근(H. Gil),강휘재(H. Kang) 한국정밀공학회 2014 한국정밀공학회 학술발표대회 논문집 Vol.2014 No.10월

돼지 난포란의 성숙과 수정에 대한 성숙배지에 첨가된 난포액의 영향

박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.2

The purpose of these experiments were to investigate the effect of follicular fluid supplemented to maturation medium on the maturation and fertilization of porcine follicular oocytes. The results obtained were as follows. In the media with MFF and LFF, the rates of maturation division of porcine oocytes in vitro were 82% and 80%, respectively, and the proportion of oocytes matured to metaphase Ⅱ become nearly constant after 39h of culture. However, the rates of maturation of porcine oocytes in medium with FCS 10% were 60% and 79% at 39 and 42h, respectively. The fertilization rate of oocyte cultured in the medium with FCS 10% is 99%. When the oocytes matured in medium with MFF and LFF were fertilized, fertilization rates were 42% and 54,%, respectively. The male pronucleus formation rates of oocytes cultured in the media with FCS 10% MFF 10% and LFF 10% concentrations were 54, 74 and 84%, respectively and were increased through culture in media supplemented with follicular fluid. The present results clearly demonstrate that follicular fluid promoted the maturation of oocytes and male pronucleus formation.

유헬스케어의료기기의 사용환경 안전성 및 성능평가를 위한 표준시험방법 연구

박수강(S. K. Park),차지훈(J. H. Cha),허찬회(C. H. Hur),박기정(K. J. Park),문인혁(I. H. Moon) 한국재활복지공학회 2010 재활복지공학회논문지 Vol.4 No.1

인구의 고령화와 국민의 소득증대에 따라 정보통신기술을 활용한 새로운 형태의 보건의료서비스인 유헬스케어에 대한 관심과 보급이 증가되어 가고 있어 이러한 용도의 유헬스 의료기기의 안전성 확보를 위한 사용환경을 고려한 안전성 및 성능 평가방법이 필요하다. 본 연구에서는 유헬스의료기기와 관련된 국제규격(IEC)과 국내규격(KS)을 참고하여 유헬스케어의료기기의 사용환경에 대한 안전성을 평가할 수 있는 기준 및 방법과 전원문제등을 고려한 필수성능을 평가할 수 있는 기준 및 방법을 개발하였다. According to graying of population and income enlargement of people, interest about U-Healthcare of new form fusing with IT technology are enlarged. Need a estimation technology and standard of test method for safety and performance considering usage environment of U-Healthcare medical device. In this research, developed the standard of test method for usage environment safety and performance of U-Healthcare medical device referring IEC standards and KS standards.

체외 배양시 과립막세포와 공배양된 돼지 난포란의 성숙과 수정

박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.1

The present study was undertaken to examine the maturation and fertilization of porcine oocytes cocultured with granulosa cells. The results obtained were as follows. The percentages of oocytes reaching metaphase Ⅱ by GC -free culture and coculture with MGC and LGC were 32, 28 and 24% at 33h, respectively. In the culture from 33h to 42h, rates of oocyte maturation showed no difference among experimental groups. The fertilization rates of oocytes matured by GC-free culture and coculture with MGC and LGC were 92, 97 and 94%, respectively, Male pronucleus formation rates of oocytes matured by GC-free culture and coculture with MGC and LGC were 49, 55 and 78% respectively and markedly increased by coculture with LGC. The present results suggest that coculture with the membrana granulosa cells does not affect the incidence of maturation, and that pronucleus formation was promoted through coculture with LGC.

페라이트계 스테인레스강의 집합조직 형성에 미치는 초기 방위 및 오스테나이트상의 영향

박수호(S. H. Park),이용득(Y. D. Lee),박찬경(C. G. Park) 한국소성가공학회 1999 한국소성가공학회 학술대회 논문집 Vol.1999 No.10