단풍취 분획물이 알콜대사효소에 미치는 영향

문형인(Hyung In Moon),지옥표(Ok Pyo Zee),문세훈(Sae Hun Mun),신말식(Mal Shick Shin) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.6

Effects of organic solvents fraction from Ainsliaea acerifolia ethanol extract on alcohol metabolism in rats were examined and the results were as follows: Ethanol souble fraction, after a single oral administration to rats, was found to cause a significant decrease in the serum ethanol concentration as well as enhancement of liver cytosolic alcohol dehydrogenase(ADH) activity, on the other hand, the fraction insouble in ethanol was found to cause an increase ethanol concentration in the blood and inhibit ADH activity.

백서에서 Depulpin^®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만,Moon, Hyung-In,Kim, Sun-Ho,Hwang, Yun-Chan,Oh, Byung-Ju,Hwang, In-Nam,Kim, Sun-Hun,Jeong, Sun-Wa,Youn, Chang,Oh, Won-Mann 대한치과보존학회 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually used for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it nay also alter the pulp tissue rendering it immunologically active, and have carcinogenic potential. Recently Depulpin$^{\circledR}$(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49 % paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue. in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present, it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin$^{\circledR}$ group The area of necrotic tissue which had no cells and fibers, was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nuclei, A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resolution and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall, was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.

담배 니코틴에 의한 사람 태아 성상세포에서 종양괴사인자(TNF-α)의 발현 억제작용

손일홍,이성익,양현덕,한선정,석승한,이재규,김재현,박주영,문형인,이성수,Son, Il-Hong,Lee, Sung-Ik,Yang, Hyun-Duk,Han, Sun-Jung,Suk, Seung-Han,Lee, Jai-Kyoo,Kim, Jae-Hyun,Park, Joo-Young,Moon, Hyung-In,Lee, Sung-Soo 대한화학회 2007 대한화학회지 Vol.51 No.3

니코틴은 사람 대식세포에서 interleukin 2 (IL-2)와 종양괴사인자 (tumor necrosis factor-alpha; TNF-α) 가 생성되는 것을 억제하는데, 이러한 억제작용은 cytokine 유전자 발현 중 전사단계에서 전사인자의 활성을 억제함으로써 일어난다. 이러한 니코틴의 면역반응 억제작용은 아프타성궤양 및 궤양성대장염, 알레르기성폐 포염, 건초열 등에서도 보고되고 있다. 만일 중추신경계에서도 위와 같은 니코틴의 면역억제 작용이 일어난 다면 다발성경화증과 같은 면역반응 매개질환의 치료에 새로운 전기가 마련될 수 있을 것이다. 본 연구에서 는 중추신경계의 여러 면역반응 매개질환의 병태생리에 대한 이해를 넓히고자, 이미 알려진 니코틴의 cytokine 생성억제가 사람 중추신경계의 성상세포에서도 일어남을 확인하고 그 억제기전을 밝히고자 하였다. 이를 위 하여 사람 태아 성상세포에 다양한 농도의 니코틴과 IL-1β를 처리한 다음 TNF-α mRNA의 발현 정도와 NF- κB의 활성을 비교, 분석하여 다음과 같은 결과를 얻었다. 1. 사람 태아 성상세포를 0.1-20 μg/ml의 니코틴으로 처리해 본 결과 10 μg/ml 이상의 농도에서 세포독성능이 나타나기 시작하였다. 2. 사람 태아 성상세포에 IL- 1β를 처리하면 2시간만에 TNF-α mRNA가 최대로 발현되었으며 그 이후로는 점진적으로 감소하였다. 3. 사 람 태아 성상세포를 1 및 0.1 μg/ml의 니코틴으로 전처리한 후 IL-1β로 자극한 군에서는 IL-1β 단독 처리군에 비해 TNF-α mRNA의 발현이 감소하는 양상을 보였다. 1 μg/ml의 니코틴을 처리한 경우에는 8시간 이후부터 TNF-α mRNA의 발현이 현저하게 감소하여 12시간에 최대로 감소하였다. 또한 0.1 μg/ml의 니코틴을 처리한 군에서는 24시간에 가장 현저하게 감소하였다. 4. 성상세포에 IL-1β로 처리한 군에서는 강력한 NF-κB의 활성 을 확인할 수 있었으며, 니코틴을 전처리하고 IL-1β 자극한 군에서는 NF-B의 활성이 감소하였다. 결론적으로 일정농도 이상의 니코틴은 세포독성효과를 나타내나 적정한 농도와 시간 경과후 니코틴은 사람 태아 성상세포에서 IL-1β에 의해 유도되는 TNF-α의 발현 감소를 유도하며, 이는 NF-κB의 활성을 감소시킴으로써 나타난다고 생각된다. The Tumor necrosis factor-α, (TNF-α), is involved in the pathogenesis of multiple sclerosis and contributes to the degeneration of oligodendrocytes as well as neurons. Nicotine has been found to have immunosuppressive and inflammation-suppressing effects. Astrocytes, the major glial cells in the CNS, are capable of producing TNF-α at both the mRNA and protein levels in response to interleukin-1 (IL-1) or TNF-α. Nicotine has been shown to influence glial cell functions. To order to explore the role of astrocytes in the production of TNF-α, astrocytes were pretreated with nicotine and are stimulated with IL-1β to determine their effects on TNF-α production. The results are as follows. Cytotoxic effects of nicotine on human fetal astrocytes were noted above 10 μg/ml of nicotine. The effect of IL-1β on TNF-α mRNA expression in primary cultured human fetal astrocytes was maximal at 2 h after IL- 1β(100 pg/ml) treatment. Human fetal astrocytes were pretreated with 0.1, 1, and 10 μg/ml of nicotine and then stimulated with IL-1β (100 pg/ml) for 2 h. The inhibitory effect of nicotine on expressions of TNF-α mRNA in human fetal astrocytes with pretreated 0.1 μg/ml of nicotine is first noted at 8 hr, and the inhibitory effect is maximal at 12 h. The inhibitory effect at 1 μg/ml of nicotine is inhibited maximal at 24 h. Nicotine at 0.1, 1 and 10 μg/ml concentrations significantly inhibits IL-1β-induced NF-κB activation. Collectively, this study indicates that nicotine might inhibit the expression of TNF-α in activated human fetal astrocytes.

오동나무꽃의 항암성분

오좌섭,지옥표,문형인,Oh, Joa-Sub,Zee, Ok-Pyo,Moon, Hyung-In 한국생약학회 2000 생약학회지 Vol.31 No.4

In search for plant-derived cytotoxic compounds, it was found that the $CHCl_3$ and EtOAC extracts obtained from the flowers of Paulownia coreana Uyeki (Scrophulariaceae) exhibited significant cytotoxic activity against human tumor cell lines, A549, SK-OV-3, SK-MEL-2, XF498, and HCT15. Activity-guided fractionation on the basis of the inhibitory activity against the growth of human tumor cell lines, in vitro, and repeated column chromatography afforded several cytotoxic compounds from P. coreana. The structures and stereochemistry of these compounds were established, on the basis of analysis of spectra including IR, UV, EI-MS, $^{1}H-NMR,\;^{13}C-NMR$ and some chemical transformations, as Compound PCCl $(2-hydroxy-4(15),11(13)-eudesmadien-8{\beta},12-olide)$, Compound $PCC2(2,3-dihydro-4-hydroxy-1(15),11(13)-xanthadien-8{\beta},12-olide)$, Compound PCE1 (chrysophanol), Compound PCE2 (emodin), Compound PCE3 (physcion). Cytotoxic activity of compounds obtained from P. coreana. on five tumor cells lines was evaluated by procedure of SRB methods.

유한요소해석을 통한 자동차용 글라스의 승강성능 예측

문형일(Hyung-il Moon),김헌영(Heon Young Kim),최천(Cheon Choi),이인혁(In Heok Lee),김도형(Do Hyung Kim) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.4

파워 글라스 시스템의 특성은 주로 윈도우 레귤레이터의 거동 특성과 글라스 런에 의한 저항에 의해 결정되며, 주로 시험 결과 분석을 통해 시스템의 성능을 예측한다. 본 논문에서는 시험적인 방법의 한계를 해결하기 위해, 익스플리시트 코드를 사용한 해석적 방법을 제안하였다. 해석 모델에 사용한 글라스 런의 마찰 계수는 다양한 조건에서의 마찰 시험을 실시하여 구하였으며, 무니-리블린 모델을 사용하였다. 또한, 윈도우 레귤레이터 파트의 메카니즘은 패스트 벨트 시스템과 슬립 링 요소를 사용하여 모델링 하였고, 상승시 발생하는 전류와 하중과의 상관관계 분석을 통해 레귤레이션 메카니즘의 신뢰성을 검증하였고, 모터의 특성을 고려하여 신뢰성 있는 글라스 상승 시간을 예측하였다. The performance of power window system was decided by driving characteristics of the window regulator part and reaction by the glass run. The performance of power window system usually has been predicted by experimental methods. In this paper, analytical method using the explicit code was suggested to overcome the limit of the experimental methods. The friction coefficient of grass run was obtained by the friction test at various conditions and the Mooney-Rivlin model was used. Also, a mechanism of window regulator consisted with the fast belt system and the slip ring elements. And, we conducted the analysis considering characteristic of a motor and obtained the lifting speed of automotive glass with high reliability.

착유우용 배합사료의 부패과정 중 발생하는 저분자 유기화합물의 분석

김용탁 ( Yong Tak Kim ),이권정 ( Kwon Jung Yi ),김겸헌 ( Gyeom-heon Kim ),김동운 ( Dong Woon Kim ),김수기 ( Soo Ki Kim ),문형인 ( Hyung-in Moon ) 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.3

In this study, we analyzed for the changes of low organic compounds during 4 weeks incubation though inoculation of harmful microorganisms on commercial feed. Two percent of overnight cultures of Exiguobacterium acetylicum, Acinetobacter calcoaceticus, Aspergillus flavus and Fusarium graminearum were inoculated in feed, respectively. After adjusting moisture level to 50% for the promotion of feed spoilage, pH was decreased to 4.58∼5.03 and microorganism was ranged to 6∼10 log₁₀CFU/g. The compounds were compare between aflatoxin G1 producing feeds and aflatoxin G1 non-producing feeds. Aflatoxins G1 were detected by the immunoaffinity column clean-up method with HPLC-FLD, and were confirmed in samples incoulated by Aspergillus flavus and Acinetobacter calcoaceticus. Koiganal II, cy-clohexanol and butadien-one were detected from samples (the non-sterilized inoculated feed) by Aspergillus flavus and Acinetobacter calcoaceticus. Respectively as aflatoxin G1 pre-detected substance, Koiganal II, cyclohexanol and butadien-one may be useful substance for the pre-detection of aflatoxin G1.

加味除濕順氣湯이 고혈압에 미치는 영향

문형권(Hyung Gwon Moon),조현경(Hyun Kyung Jo),유호룡(Ho Ryong Yu),설인찬(In Chan Seol),김윤식(Yoon Sik Kim) 한의병리학회 2006 동의생리병리학회지 Vol.20 No.4

최근 3년간 대구 달성군 보건소 한방진료실의 진료현황에 대한 통계적 연구

문형권 ( Hyung Gwon Moon ),설인찬 ( In Chan Sul ),김윤식 ( Yoon Sik Kim ) 대전대학교 한의학연구소 2005 혜화의학회지 Vol.14 No.2

유한요소해석을 통한 자동차용 글라스의 승강성능 예측

문형일(Hyung-il Moon),김헌영(Heon Young Kim),최천(Cheon Choi),이인혁(In Heok Lee),김도형(Do Hyung Kim) 대한기계학회 2010 大韓機械學會論文集A Vol.34 No.11

파워 글라스 시스템의 특성은 주로 윈도우 레귤레이터의 거동 특성과 글라스 런에 의한 저항에 의해 결정되며, 시험 결과 분석을 통해 시스템의 성능을 예측한다. 본 논문에서는 시험적인 방법의 한계를 해결하기 위해, 익스플리시트 코드를 사용한 해석적 방법을 제안하였다. 해석 모델에 사용한 글라스 런은 무니-리블린 모델을 사용하여 모델링 하였고, 다양한 조건에서의 마찰 시험을 실시하여 마찰계수를 구하였다. 또한, 윈도우 레귤레이터 파트의 메커니즘은 패스트 벨트 시스템과 슬립 링요소를 사용하여 모델링 하였고, 상승 시 발생하는 전류와 하중과의 상관관계 분석을 통해 레귤레이션 메커니즘의 신뢰성을 검증하였고, 모터의 특성을 고려하여 신뢰성 있는 글라스 상승 시간을 예측하였다. The performance of power window system was decided by driving characteristics of the window regulator part and reaction by the glass run. The performance of power window system usually has been predicted by experimental methods. In this paper, an analytical method using the explicit code was suggested to overcome the limit of the experimental methods. The friction coefficient of glass run was obtained by the friction test at various conditions and the Mooney-Rivlin model was used. Also, a mechanism of window regulator consisted of the fast belt system and the slip ring elements. And, we conducted the analysis considering characteristic of a motor and obtained the lifting speed of automotive glass with high reliability.

그늘쑥의 식물화학적 성분

문형인,권학철,지옥표,이강노,Moon, Hyung-In,Kwon, Hak-Cheol,Zee, Ok-Pyo,Lee, Kang-Ro 한국생약학회 1999 생약학회지 Vol.30 No.1

Two carotenoids and four flavonoids were isolated from the whole plant of Artemisia sylvatica Max. Their structures were identified as ${\beta}-carotene$, lutein, luteolin, rutin, $pectolinarigenin-7-O-{\beta}-glucopyranoside$ and luteolin-7-O-neohesperidoside on the basis of spectroscopic means.