FDM(Fused Deposition Modeling)방식을 이용한 다공성 격자구조체 개발 및 치과보철물 분야로의 응용

김한울(Han Wool Kim),배은정(Eun Jung Bae),임중연(Jung Yeon Lim) 대한기계학회 2017 대한기계학회 춘추학술대회 Vol.2017 No.11

보리발효추출물로부터 분리한 다당의 대식세포 활성화 및 신호 전달

김한울(Han Wool Kim),지희숙(Hee Sook Jee),신광순(Kwang-Soon Shin) 한국식품과학회 2018 한국식품과학회지 Vol.50 No.5

보리는 높은 비율로 식이섬유를 함유하고 있어, 일반적으로 영양학적으로는 전곡 형태로의 섭취가 추천되고 있지만, 조직감과 소화율을 고려하여 발효물 형태의 소비가 바람직한 것으로 판단되고 있다. 본 연구는 효소당화 후, 효모 및 유산균 발효를 거쳐 조제한 보리발효물로부터 조다당 BF-CP를 분리하고, 대식세포에 대한 면역증강 효과 및 세포 내 신호전달을 규명하여 기능성 소재로의 이용방안을 모색하기 위해 계획되었다. BF-CP 획분의 일반화학적 특성을 분석한 결과 70.7% 글루코스 11.4% 자일로스 및 9.0% 아라비노스를 포함하여 91.1%의 중성당으로 이루어진 중성다당이었다. BF-CP는 RAW 264.7 대식세포주에서 농도의존적으로 IL-6, TNF-α와 같은 사이토카인 및 NO의 생산능을 유도하는 등 높은 대식세포 활성능을 나타냈다. 또한 qPCR 분석을 통해, BF-CP 획분을 대식세포주에 처리하였을 때, 처리 농도에 비례하여 IL-6, TNF-α 및 iNOS의 mRNA 유전자 발현을 증가시킴을 확인할 수 있었다. 한편 Western blot을 활용한 신호전달 단백질 추적실험에서 BF-CP 획분을 대식세포주에 처리하였을 때, JNK, ERK 및 p38과 같은 MAPK 경로와 NF-κB 경로의 관련 단백질을 인산화시킴이 확인되었으며 그 활성은 BF-CP 농도에 의존적이었다. 이상의 결과로부터 보리발효물 유래 다당 BF-CP는 MAPK와 NF-κB 경로를 통해 대식세포를 활성화시키며, 이를 통하여 NO, IL-6 및 TNF-α와 같은 면역활성화 관련 물질의 생산을 높은 비율로 유도시킨다는 것을 최종 확인할 수 있었다. Barley has nutritional benefits due to its high dietary fiber content; therefore, the intake of whole barley grains is recommended. However, barley is often consumed in the fermented form because of the improved texture and digestibility. The present study was designed to elucidate the intracellular signaling pathway for macrophage activation by the polysaccharide BF-CP from fermented barley. BF-CP is a neutral polysaccharide, composed of neutral sugars, including glucose (70.7%), xylose (11.4%), and arabinose (9.0%). BF-CP exhibited macrophage-stimulatory activity by inducing the production of interleukin (IL)-6, tumor necrosis factor (TNF)-α, and nitric oxide in RAW 264.7 macrophages. Further, BFCP treatment strongly increased the IL-6 and TNF-α gene expression in a concentration-dependent manner. Signal transduction experiments using immunoblotting showed that BF-CP phosphorylated mitogen-activated protein kinases (MAPKs), such as c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38, and nuclear factor (NF)-κB, in RAW 264.7 cells in a concentration-dependent manner. These results suggest that BF-CP activates the macrophages via MAPK and NF-κB pathways, and also induces an increase in the production of cytokines.

딥러닝을 이용한 컨트롤 암의 위상최적화 알고리즘 개발

김한울(Han-wool Kim),민승재(Seungjae Min) 한국자동차공학회 2021 한국자동차공학회 부문종합 학술대회 Vol.2021 No.6

음원자료에 기록된 향제 삼현육각 및 무속거상악의 전승자 -이보형 채록 <삼현육각>과 <시나위>의 음원자료를 중심으로-

김한울(Kim, Han-wool) 한국음악문화학회 2019 한국음악문화연구 Vol.13 No.-

전통사회에서 삼현육각은 관아와 민간에 널리 쓰이던 음악이다. 그러나 근대 이후 신식관청과 새로운 음악의 등장 등 사회적 변모로 인해 공식적인 수요가 줄어들게 되었다. 이로 인해 삼현육각을 담당하는 음악집단의 해체 및 이 음악을 기억하는 이들 또한 점차 사라기제 뙤었다. 1979년 이보형의 주도로 전국의 삼현육각 조사가 실시되었고, 당시 현장조사를 통해 녹음된 삼현육각 연주와 대담은 한국고음반연구회에서 1995년 이보형 채록 <삼현육각>과 2004년 이보형 채록 <시나위>의 음반으로 발매되었다. 이에 따라 본고에서는 이 두 음반 자료를 중심으로 향제 삼현육각 및 무속거상악의 전승자에 대해 살펴보고자 하였다. 이보형 채록 <삼현육각>음반 중 <염불, 굿거리, 타령, 자진타령, 승전곡>을 통해 전주삼현육각의 전승자와 그 음악에 대해 확인하였으며, 이보형 채록 <시나위>음반의 <염불, 굿거리>, <긴염불>, <타령>, <굿거리>를 통해 영암 삼현육각을 살펴보았다. 또한 영광 삼현육각과 전승자에 대해서는 <할림청 시나위>, <허튼타령>을 통해 확인 할 수 있었다. 그리고 위의 음원자료에 수록되지 않았으나 화순 능주 씻김굿에서의 혼맞이 굿 절차와 영광의 법성포 단오제 용왕제에서 <남도거상악>을 찾아 볼 수 있었다. 이처럼 삼현육각은 각 지역에 독창적인 문화를 형성해 온 귀중한 자산이다. 그러나 현재 당대의 음악가들이 연로하시거나 작고하셨기 때문에 남겨진 삼현육각을 기록하고 계승하는 것이 중요하다고 생각한다. In traditional society, Samhyeonyukgak was music widely used in government offices and the private sector. This led to the disbandment of the music group in charge of the Samhyeonyukgak and the gradual disappearance of those who remember the music. However, official demand has decreased since the modern era due to the emergence of the new government and new music groups. In 1979, the nationwide Samhyeonyukgak survey was conducted under the leadership of Lee Bo-hyung. The music and dialogue of Samhyeonyukgak, recorded through an on-site survey at the time, were made into Samhyeon Yukgak in 1995 and Shinawi Music Album in 2004. Therefore, in this paper, wanted to examine the successor to the Hyangje Samhyeonyukgak and Shamanism Geosangak, focusing on Lee Bo-hyung"s records, Samhyeonyukgak and Sinawe. Among Lee Bo-hyung"s records, Jeonju Samhyeonyukgak was able to check out the music at Yeombul, Gutgeori, And among Lee Bo-hyung"s Sinawi records, looked at Yeongam Samhyeonyukgak through Yeombul, Gutgeori, Ginyeombul, Taryeong and Gutgeori. In addition, it was possible to find out about Yeonggwang Samhyeonyukgak and successor through HalimCheong shinawi and Heoteun Taryeong. Although it was not included in the above album, could find Namdo Geosangak Music in the procedures of the Hwashun Neungju Gut a shaman ritual for cleaning dead person"s soul and the procedure of Gut and the Yongwangje of the Yeonggwang Beopseongpo Danoje. As such, Samhyeonyukgak is a valuable asset that has formed an original culture in each region. But now, since the musicians of the time were old or dead, I think it is important to record and inherit Samhyeonyukgak.

스포츠클라이밍 선수들의 집중 코어운동이 스트레스지표와 기능체력에 미치는 영향 : 사례 연구

김한울 ( Kim Han-wool ),서영환 ( Seo Young-hwan ) 대한체육학회(구 한국유산소운동과학회) 2020 대한스포츠융합학회지 (jcses) Vol.18 No.2

The purpose of this study is to find out how stress indicators that impede the function of exercise and functional physical strength, which have the characteristics of sports climbing events, affect sports climbing athletes through intensive core exercise. It consisted of a total of 8 weeks, 4 times a week, 80 minutes a day, and the intensity of exercise was divided into 1 to 4 weeks and 5 to 8 weeks to set the intensity. The data processing was conducted by 8 elite sports climbing athletes registered in the Korea Sports Association, and the following conclusions were drawn from the contents of the results measured before and after exercise. There were statistically significant differences in muscle strength, muscular endurance, and quickness in the functional fitness factors of sports climbing athletes. In addition, there was a statistically significant difference in the change of the stress index of the exercise group of sports climbing athletes. Putting the above results together, as a result of intensively performing core exercise on sports climbing athletes, it was shown that the result of physically reducing stress indicators and increasing functional physical strength. If you set an appropriate period during the rest period, training period, competition period, and recovery period, it will be an exercise program that can help improve performance by obtaining better results.

친환경 무기항균제 개발 및 효능 연구

김한울(Kim, Han-Wool),김소형(Kim, So-Hyung),박선영(Park, Sun-Young),이미영(Lee Mi-Young) 한국산학기술학회 2006 한국산학기술학회논문지 Vol.7 No.5

The environmentally benign inorganic bacteriocide was developed by using waste shell as a matrix and its efficacies against various bacteria were examined in this investigation. The inorganic bacteriocide inhibited strongly the growths of various bacteria such as S.aureus and E.coli. The inorganic bacteriocide also showed strong killing effect on the growth of P.acnes which causes the acne on human face. Moreover, this bacteriocide inhibited the activity of Iipoxygenase prominently, indicating notable anti-inflammatory activity of the bacteriocide.

Tuberculous Empyema Necessitatis with Osteomyelitis, a Rare Case in the 21^st Century

김한울,임고운,조혜경,이현주,원태희,박경운,김경효,Kim, Han Wool,Lim, Goh-Woon,Cho, Hye Kyung,Lee, Hyunju,Won, Tae Hee,Park, Kyoung Un,Kim, Kyung-Hyo The Korean Society of Pediatric Infectious Disease 2011 Pediatric Infection and Vaccine Vol.18 No.1

흉벽 천공성 농흉(Empyema necessitatis)은 농흉이 흉막 외의 공간으로 확장되어 나간 것을 말한다. 결핵성 흉벽 천공성 농흉은 결핵의 드문 합병증으로서 특히 소아에서는 더욱 드물다. 본 저자들은 결핵에 노출된 병력이 없던 21개월 남자아이에서 7번째 늑골의 골수염에 동반된 결핵성 흉벽천공성 농흉을 경험하여 이에 대해 보고한다. 우리는 환자의 진단과 치료를 위해 수술적 치료를 시행하였고, 조직을 이용한 PCR 및 분자생물학적 검사에서 M. tuberculosis complex를 확인하여 항결핵제를 이용해 합병증 없이 치료하였다. Empyema necessitatis refers to empyema that extends into the extrapleural space through a defect in the pleural surface. Tuberculous empyema necessitatis is a rare complication of tuberculosis. We experienced a 21-month-old boy with tuberculous empyema necessitatis with osteomyelitis in the right $7^{th}$ rib. He presented with a mass on the right lateral chest wall, which was soft and nontender, enlarging for one month. He also had mild fever. The plain radiograph of his chest revealed soft tissue swelling and calcified lymph node on the left axilla, and his PPD skin test was positive. CT scan of the chest showed empyema necessitatis at the right lower chest and upper abdominal walls with osteomyelitis of the right $7^{th}$ rib. He did not have concurrent pulmonary tuberculosis. Surgery was performed for diagnosis and treatment. In histopathologic findings, chronic granulomatous inflammation with caseation necrosis was shown and was positive for acid fast bacilli stain. In addition, M. tuberculosis complex was found as etiology by polymerase chain reaction. The patient has been treated with anti-tuberculous medication without any specific complication.

인도에서 귀국한 가족에서 발생한 심한 고혈당 동반 소아 뎅기열 1례

김준영,김한울,Kim, Joon Young,Kim, Han Wool 대한소아감염학회 2020 Pediatric Infection and Vaccine Vol.27 No.2

Dengue fever (DF) is endemic throughout tropical and subtropical regions; however, it is not endemic in Korea. Imported infectious diseases should be suspected when encountering returned travelers with non-specific symptoms such as fever, rash, and headache. Persistent vomiting, abdominal pain, hemorrhagic tendency, hepatic enzyme abnormalities, and proteinuria are risk factors for the development of severe DF in a patient with DF. Severe hyperglycemia is also known to be related to poor prognosis in acute illness, and with certain underlying diseases that present with hyperglycemia, such as diabetes, it is related to the progression of severe DF. We report a DF case with severe hyperglycemia in an adolescent returning from India. Pediatric DF cases reported in Korea were also reviewed.

RF Power에 따른 태양전지용 N-type ZnS 특성연구

양현훈,김한울,정운조,이석호,소순열,박계춘,이진,정해덕,Yang, Hyeon-Hun,Kim, Han-Wool,Jeong, Woon-Jo,Lee, Suk-Ho,So, Soon-Youl,Park, Gye-Choon,Lee, Jin,Chung, Hea-Duck 한국전기전자재료학회 2011 전기전자재료학회논문지 Vol.24 No.7

In this study, we use the $2.5cm{\times}7.5cm$ soda lime glass as the substrate. We used the ultrasonicator. Glass was dipped in the acetone, methanol and DI water respectively for 10 minutes. Ar(99.99%)gas was used as the sputtering gas. We varied the RF power between 100~175 W with 25 W steps. Base pressure was kept by turbo molecular pump at $3.0{\times}10^{-6}$ torr. Working pressure was kept by injection of Ar gas. ZnS thin films were deposited with the radio frequency magnetron sputtering technique at various temperatures and sputtering powers. It is also clearly observed that, the intensity of the (111) XRD peak increases with increasing the RF power. Electrical properties were measured by hall effect methods at room temperature. The resistivity, carrier concentration, and hall mobility of ZnS deposited on glass substrate as a function of sputtering power. It can be seen that as the sputtering power increase from 100 to 175 W, the resistivity of the films on glass decreased significantly from $8.1{\times}10^{-2}$ to $1.2{\times}10^{-3}\;{\Omega}{\cdot}cm$. This behavior could be explained by the effect of the sputtering power on the mobility and carrier concentration. When the RF power increases, the carrier concentration increases slightly while the resistivity decreases significantly. These variation originate from improved crystallinity and enhanced substitutional doping as the sputtering power increases.

소아청소년 코로나바이러스감염증-19: 역학과 임상 양상

최수한,김한울,강지만,김동현,조은영,Choi, Soo-Han,Kim, Han Wool,Kang, Ji-Man,Kim, Dong Hyun,Cho, Eun Young 대한소아감염학회 2020 Pediatric Infection and Vaccine Vol.27 No.1

2019년 12월 발견되어 2020년 3월 11일 전 세계 대유행이 선포된 코로나바이러스감염증-2019 (coronavirus disease-2019, COVID-19)는 신종 감염병으로서 수많은 감염사례와 사망을 초래하여 사회 전반적으로 영향을 끼치고 있다. 소아청소년 COVID-19는 발병 환자 중 차지하는 비율이 적으며 성인보다는 경증인 경우가 많으나, 일부에서는 중증으로 진행할 수 있다. 신생아 시기부터 감염될 수 있으며, 지역사회 감염의 전파자로서 역할을 할 수 있을 것으로 보인다. 본 종설에서는 현재까지 소아청소년에서의 COVID-19에 대해 알려진 내용을 정리하여 소아청소년 환자들의 진료에 도움을 주고자 한다.