지구성 운동 유발 전 처치에 따른 심장보호 효과 (iNOS knock-out mice 활용)

김철현 ( Chul Hyun Kim ),엄현섭 ( Hyun Sub Um ),이영익 ( Young Ik Lee ),김윤만 ( Yoon Man Kim ),조인호 ( In Ho Cho ),김명기 ( Myung Ki Kim ),이규성 ( Kyu Sung Lee ),양대승 ( Dea Seung Yang ),오윤선 ( Yoon Sun Oh ),오유성 ( Yoo Sun 한국운동영양학회 2005 Physical Activity and Nutrition (Phys Act Nutr) Vol.9 No.1

The goal of this study was to explore the role of inducible NOS (iNOS) and the delayed protection of exercise preconditioning from the relative myocardial ischemia reperfusion injury. Mice were randomly placed in one of five testing groups: wild-type mice group (n = 15), high-intensity wild-type mice acute exercised group (n = 6). low-intensity wild-type mice acute exercised group (n = 5), L-NAME + wild-type mice acute exercised group (11 = 5), and homozygous (-/-) iNOS gene knock-out mice acute exercised group (n = 5). All acute exercised groups ran on the treadmill (0% grade) at 65 m,`min as a high-intensity exercise and at 27-30 m/min as a low-intensity exercise. The results showed that all acute exercise significantly reduced magnitude of a myocardial infarction in biphasic manner. In accordance with these data, NFKB and HSP-70 protein expression was increased at 24 after exercise-induced preconditioning (PC) in both low-intensity wild-type mice acute exercised group and homozygous (-/-) iNOS gene knock-out mice acute exercised group compared with wild-type mice group. In contrast, neither endothelial nitric oxide synthase (eNOS) nor inducible nitric oxide synthase (iNOS) expression changed at both wild-type mice group and low-intensity wild-type mice acute exercised group. In conclusion, The strong evidence of this study indicates that HSP-70 is particulary cytoprotectiye against protein-damaging stress such as I-R. Also, this study demonstrate that the late phase of exercise-induced PC is not associated with up-regulation of eNOS and iNOS but infarct-sparing effect and mild up-regulation of NFKB and HSP-70 at the late phase of exercise-induced PC in wild-type mice and iNOS gene knock-out mice. Thus, this study identifies a specific protein that mediates late PC in vitro.

엘리트 운동선수에서 부위별 다주파수 생체 전기 임피던스법(SMF-BIA)에 의한 체지방 추정의 유효성

김철현(Chul Hyun Kim) 한국사회체육학회 2008 한국사회체육학회지 Vol.0 No.34

The purpose of this study was to exam the accuracy and availability of SMF-BIA to predict percent body fat(%fat) of elite athletes in Korea. 183 elite athletes (107 male, 76 female) participated on the present study. Interview was administered for finding disease and any change of body composition, and than tests were proceeded including hieght, weight, SMF-BIA, and DEXA. The data of predicted %fat by SMF-BIA and measured %fat by DEXA were analyzed by the simple linear regression analysis, T-test, and Bland-Altman methods. The results showed that the correlation coefficient between predicted %fat and measured %fat was r=0.757 and r=0.667 in male and female respectively. Also, the slopes for the line of identity and y-intercepts were significantly different from the zero or one in both male and female. However, the total errors were 2.3%fat in male and 2.6%fat, which were evaluated as `very good`. In the Bland-Altman plot, the residuals in male had a negatively correlation with the mean of %fat from SMF-BIA and DEXA, but not in female. The results suggested that the regression equation of %fat by SMF-BIA was low in accuracy. Especially, use of SMF-BIA in male athletes should be great careful because of existence of systemic errors.

증례 : 급작한 심장돌연사로 발현된 남자 선천성 QT 간격 연장 증후군 1예

김철현 ( Chul Hyun Kim ),김대혁 ( Dae Hyeok Kim ),임주한 ( Joo Han Lim ),최윤아 ( Yun Ah Choi ),권준 ( Jun Kwan ),박금수 ( Keum Soo Park ),이우형 ( Woo Hyung Lee ) 대한내과학회 2007 대한내과학회지 Vol.72 No.1S

비즈니스 모델 특허 분석을 통한 핵심 서비스 기술 파악

김철현 ( Chul Hyun Kim ) 한국경영공학회 2015 한국경영공학회지 Vol.20 No.2

This study suggests a new approach to identify core service technologies through business model patent analysis. First, technological interrelationship matrices of intensity, relatedness, and cross-impact perspectives are constructed with support, lift and confidence values calculated by conducting an association rule mining on the co-classification information of business model patent data. Second, the analytic network process is applied to the constructed technological interrelationship matrices in order to produce the importance values of service technologies from each perspective. Finally, data envelopment analysis is employed to the derived importance values in order to identify priorities of service technologies, putting three perspectives together. A case study of mobile service technology is conducted to illustrate the process of executing and utilizing the proposed approach. It is expected that suggested approach could help technology planners of service firms to formulate strategy and policy for service innovation.

가소화 처리 목편으로부터 재조된 복합재료의 물성

김철현(Chul-Hyun Kim),김강재(Kang-Jae Kim),엄태진(Tae-Jin Eom) 한국펄프·종이공학회 2008 펄프.종이기술 Vol.40 No.3

모바일 플랫폼의 MDA 적용 방안

김철현 ( Chul-hyun Kim ),이동수 ( Dong-su Lee ),이민태 ( Min-tae Lee ),김병기 ( Byung-ki Kim ) 한국정보처리학회 2007 한국정보처리학회 학술대회논문집 Vol.14 No.1

본 논문에서는 Model Driven Architecture를 다양한 모바일 플랫폼에서 적용하는 방안에 대해 설명한다. 모바일 플랫폼은 Symbian OS, Microsoft Windows CE 등 다양한 종류가 있으며, 이들의 어플리케이션을 재사용하기 위해서는 각 플랫폼에 맞는 언어로 다시 개발해야 한다. MDA는 이러한 이기종의 플랫폼에 적용할 수 있는 가장 효율적인 아키텍처이다. PIM 모델을 작성하고 변환규칙을 적용한 자동화 도구로써 PSM 모델과 소스코드까지 자동으로 생성이 가능하기 때문에 높은 개발 생산성과 이식성, 상호운용성을 제공할 수 있다.

Polygenic Association of ACE and ACTN3 Polymorphisms with Korean Power Performance

Chul-Hyun Kim(김철현) 한국생명과학회 2012 생명과학회지 Vol.22 No.3

이 연구는 한국인에서 적용할 수 있는 근력관련 유전적 소인을 ACE 유전자와 ACTN3 유전자를 단일유전자 수준과 다중유전자 수준에서 관계성을 규명하는데 목적이 있다. 연구의 목적을 위해 근력운동종목의 엘리트선수 158명, 국가대표선수 106명, 대조군 676명을 동원하여 ACE ID 다형성과 ACTN3 R577X 다형성 분포를 분석했다. 연구결과, ACE 다형성에서 II 유전형 및 I 대립형질은 유의하게 높은 분포를 가졌고, 반면 DD 유전형 및 I 대립형질은 유의하게 낮은 분포를 가졌다(Top-Class vs. Control: 41.4% vs. 32.1 for II genotype, 67.1% vs. 57.7% for I allele, p<0.05). ACTN3 다형성에서 RR 유전형 및 R 대립형질은 유의하게 높았고 XX 유전형 및 R 대립형질은 유의하게 낮았다(Top-Class vs. Control: 42.3% vs. 29.0 for RR genotype, 65.3% vs. 54.8% for R allele, p<0.05). 다중유전자 수준에서 근력은 ACE 다형성과 ACTN3 다형성이 조합된 우성조합유전형(II/ID+RR/RX)이 최우수 경기력에서 유의하게 높은 분포를 가졌다(Top-Class vs. Control: 82.9% vs. 66.7% for II/ID+RR/RX, p<0.05). 또한 최우수 경기력을 가진 국가대표는 엘리트와 대조군 보다 유의하게 높은 TGS를 가졌다(66±0.9 vs. 58±1.9 vs. 56±2.3, p<0.05). 이를 근거로 우성조합유전형이 최우수 근력 경기력을 가질 가능성에 대한 승산비는 2.43배(CI:1.45-4.09, p<0.001)였다. 따라서 ACE 다형성과 ACTN3 다형성은 한국인에서 근력과 관계된 유전형으로 확인되었으며, 두 유전자는 상호 조합된 다중유전형에서 근력 경기력에 영향을 줄 것으로 사료된다. 또한 ACE 다형성과 ACTN3 다형성을 조합한 다중유전자는 근력 경기력을 예측할 수 있은 유전적 요인으로 사료되었다. This study aimed to examine whether the polygenic profile of ACE ID and ACTN3 R577X polymorphisms is associated with muscle power performance in Korean athletes. For this study, 106 top-class power athletes (top-class group), 158 elite power athletes (elite-class group), and 676 healthy adults (control) aged 18-39 yrs were recruited and their genotypes were analyzed. The top-class group showed higher frequencies of the II genotype and I allele in ACE, as well as higher frequencies of the RR genotype and R allele in ACTN3 (top-class vs. control: 41.4% vs. 32.1% for II genotype, 67.1% vs. 57.7% for I allele, p<0.05; 42.3% vs. 29.0% for RR genotype, 65.3% vs. 54.8% for I allele, p<0.05). In the polygenic profile, the top-class group had significantly higher frequencies of combined- II/ID+RR/RX genotype than the control group (top-class vs. control: 82.9% vs. 66.7% for II/ID+RR/RX, p<0.05), and there was even a sharp increase in total genotype score (TGS) in this group compared to the elite-class and control groups (66±0.9 vs. 58±1.9 vs. 56±2.3, p<0.05). The combined- II/ID+RR/RX genotype showed the possibility of succussion in the top-class muscle power performance with an odds ratio of 2.3 (CI:1.4-4.1, p<0.05). These results suggested that ACE and ACTN3 need to interact with each other to affect muscle-power performance in an additive form. Furthermore, the polygenic profile of ACE and ACTN3 can predict muscle performance with high success in a homogeneous dominant combined genotype (II/ID+RR/RX). A further study could identify and combine other genes into ACE and ACTN3 for muscle strength.

ω-3계 지방산의 미세캡슐화에 관한 연구

김철현(Chul-Hyun Kim),이경욱(Kyung-Wook Lee),백승천(Seung-Chun Baick),곽해수(Hae-Soo Kwak),강종옥(Jong-Ok Kang) 한국식품과학회 1996 한국식품과학회지 Vol.28 No.4

한국인에서 무산소근파워와 ACE I/D 다형성간의 관계

김철현 ( Chul Hyun Kim ) 한국스포츠정책과학원(구 한국스포츠개발원) 2007 체육과학연구 Vol.18 No.3

이 연구의 목적은 ACE I/D 다형성이 무산소근파워와 관련한 유전자인가를 구명(究明)하는 것이다. 연구목적을 위해 집단유전학의 사려-대조 연구법을 적용하였으며, 이 때 동원된 사례군은 무산소근파워 체력요인이 절대적으로 필요한 STMP(Short-term muscle power) 종목에 해당하는 육상 100m, 200m 단거리와 역도 종목의 우수선수 87명을 동원하였고, 대조군은 외관상 건강한 20대 30대 남녀 일반인 643명을 동원하여, ACE I/D 다형성을 비교 · 분석하였다. 연구결과 STMP 선수들의 ACE I/D 다형성 유전형 분포는 II 유전형이 42.5%, ID 유전형이 51.7%, DD 유전형이 5.7%로 대조군인 일반인의 II 유전형 31.7%, ID 유전형 51.6%, DD유전형 16.7% 분포와는 유의한 차이를 가졌다(P<.05). STMP 선수들은 대조군에 비해 DD 유전형이 유의하게 낮았으며(P<.05), 반면 II + ID 유전형이 높은 분포율을 나타냈다(P<.05). 이를 근거로 무산소근파워 종목에 대한 성공 수준을 예측하고자 승산비를 구한 결과, II + ID 유전형이 DD 유전형 보다 평균 3.3배 및 최소 1.3배 수준의 성공 가능성을 가졌다(P<.01). 이 연구결과 한국인에서 ACE I/D 다형성은 II 유전형과 ID 유전형이 무산소근파워 능력의 잠재성을 높게 가지는 무산소근파워성 유전형인 것으로 나타났다. The D allele of the ACE I/D polymorphism is associated with elevated levels of serum and tissue ACE, increased production of angiotensin II. Several cardiac and renal conditions as well as skeletal muscle conditions appear to have a worse prognosis in subject homozygous for the D allele, whereas the I allele has been associated with enhanced performance. This study investigated the association between ACE I/D polymorphism and STMP(short-term muscle power) in 87 outstanding elite athletes (100m, 200m distance running weight-lifting) and 643 healthy people. Distributions of the genotype of athletes are 42.5% of II genotype, 51.7% of ID genotype, and 5.7% of DD genotype, and different from that in controls which are 31.7%, 51.6%, 16.7% for II, ID, DD genotype respectively. The STMP significantly had 11.0% higher in II genotype than the control. The odds ratio associated with II+ID genotype was 3.3 time on average and ranged form 1.3 to 8.27 compared to DD genotype. In conclusion, in a geographically isolated homogenous population of Korea STMP athletes and healthy adults the presence of the DD genotype of the ACE gene conferred no increase in success in STMP sports, but the presence of the II genotype of the ACE gene suggested an increase in success in STMP sports.

ACTN-3 유전자 다형성과 무산소성 파워 능력

김철현 ( Chul Hyun Kim ),이영익 ( Young Ik Lee ),김윤만 ( Yoon Man Kim ),조인호 ( In Ho Cho ),제임스전 ( James Jeon ),김지연 ( Ji Yeon Kim ),박재현 ( Jae Hyun Park ),김혜진 ( Hea Jin Kim ),신경아 ( Kyung A Shin ),이승주 ( Seong Ju 한국운동영양학회 2004 Physical Activity and Nutrition (Phys Act Nutr) Vol.8 No.3

The α-actinin is an actin-binding protein belonging to the spectrin protein superfamily. a-actinin-3(ACTN-3) expression is limited to skeletal muscle, especially Type Ⅱ muscle fiber. This gene has homozygosity for a premature stop codon resulting in a-actinin-3 deficiency. The deficiency in the type Ⅱ muscle is able to be compensated by a-actinin-2(ACTN-2). While that deficiency does not induce a disease phenotype, the ACTN-3 is highly conserved in evolutionary terms because of its functions independent of the ACTN2. Researchers have suggested that this trait is related to muscle function at the extremes of power performance. Therefore, we compared the relative frequencies of the ACTN-3 R577X polymorphism between anaerobic power athletes and control group. For this study, we recruited 158 sprint or power elite athletes and 414 healthy adults. The results of the current study showed significant differences in the genotype frequencies such that elite anaerobic power athletes have 4% higher of the RR genotype and 9% lower of the XX genotype than the healthy adults. With respect to the allele frequencies, the athletic group had significantly higher R allele frequency and significantly lower X allele frequency than the control group. In summary, these results suggest that the ACTN-3 R577X genotype may represent a genetic marker for anaerobic power performance.