http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Thiodiglycol를 분해하는 Cupriavidus sp.의 분리와 특성
박종덕,김지천,윤기홍,Park, Jong-Deok,Kim, Jee-Cheon,Yoon, Ki-Hong 한국미생물학회 2007 미생물학회지 Vol.43 No.4
탄소원으로 thiodiglycol(TDG)을 함유한 배지에서 농후배양하여 인삼토양으로부터 TDG 분해균을 분리하였다. 분리균 WS-32의 형태적, 생화학적, 유전학적 특성을 조사한 결과 분리균이 Alcaligenes faecalis와 유사한 생화학적 성질을 지니고 있으며, 16S rRNA 서열이 Cupriavidus 속 균주와 유사도가 높은 균주로 판명되었다. WS-32는 $33^{\circ}C{\sim}37^{\circ}C$, pH $6.0{\sim}8.0$에서 성장이 우수하였으며, TDG에 의해 성장에 약간의 저해를 받지만, 배양후기에서는 이를 탄소원으로 이용하는 현상을 보였다. HPLC를 통해 배양액 내 잔존하는 TDG를 분석한 결과 2일 배양하였을 때 배지내 잔존하는 TDG가 상당량 감소한 것으로 확인되었다. 분리균 WD-32의 균체 파쇄상등액은 TDG의 산화활성을 보였으며, pH 8.0과 $45^{\circ}C$에서 산화활성이 가장 높았다. A Gram-negative bacterium capable of degrading thiodiglycol (TDG), main hydrolysis product of sulfur mustard, was isolated from ginseng field in enrichment medium supplemented with TDG as carbon source. The isolate, WS-32, grew optimally at $30-37^{\circ}C$ and pH 6.0-8.0. It was found to be similar to the genus Cupriavi연 on the basis of 165 rRNA sequence, while its biochemical properties were highly homologous to Alcaligenes faecalis. The cell growth of WS-32 strain was slightly inhibited on LB broth by TDG, but the maximum level of its growth was maintained stably in the presence of TDG. After incubation of inoculated LB medium or uninoculated LB medium containing TDG for 2 days, TDG amount of the culture filtrate was analyzed to decrease noticeably by HPLC. TDG and alcohols were also oxidized by cell-free extract of the isolate with maximum activities at pH 8.0 and $45^{\circ}C$.
Detection of Salmonella Group with Monoclonal Antibodies
차승희,오상곤,김지천,윤희주,정윤수,Cha, Seung-Hee,Kim, Sang-Kon,Kim, Jee-Cheon,Yun, Hee-Ju,Jung, Yun-Su Korean Society for Biochemistry and Molecular Biol 1989 한국생화학회지 Vol.22 No.1
열처리된 Salmonella typhimurium 세포를 이용하여 Salmonella에 대한 단일클론성 항체를 생산하는 hybridoma 3종을 얻었다. 3종의 hybridoma는 각각 다른 항체 IgG1 k, IgG2a k, IgM을 생산하였으며 이들 항체는 EIA로 Salmonella와 다른 세균들에 대한 특이성을 검토하였다. IgG1 k 항체는 시험된 Salmonella균과 Enterobacter clocae 및 몇종의 E. coli strain과 친화성이 있었으며 IgM항체는 대부분의 세균에 대해서 친화성을 나타내었다. IgG2a k 항체는 Salmonella strain과 E. coli strain B에 약한 친화성을 나타내었다. IgG2a k 항체는 modified double antibody sandwich EIA에서 Salmonella 항원을 $5{\times}10^3cells/ml$ 농도까지 검출 가능하였으며 항체역가는 mouse 복수액에서 $5{\times}10^4$이었다. Monoclonal antibodies were produced against whole cells of heat treated Salmonella typhimurium. From the Balb/c mice immunized, 164 hybridomas were obtained. Of 164 hybridomas, six reacted with all three species of Salmonella; three were cloned. Monoclonal antibodies from three hybridomas were characterized to belong to IgG1 k, IgG2a k, IgM subclass respectively. Among them, IgG2a k revealed the most specific reactivity of Salmonella species tested, even though reacted weakly with one of E. coli strains, whereas IgGl k and IgM were found to cross react with some strains of Enterobacteriaceae. By using a modified double antibody sandwich EIA together with IgG2a k, a minimum level of cells of Salmonella species detected was estimated $5{\times}10^3$ cells/ml. Antibody titer of IgG2a k in ascite fluids was $5{\times}10^4$.
Physostigmine 과 Procyclidine 으로 구성된 복합예방제의 유기인제 해독효능
허경행(Gyeung Haeng Hur),천기철(Ki Cheol Cheon),피택산(Taek San Phi),김지천(Jee Cheon Kim),홍대식(Dea Sik Hong),박훈(Hoon Park),정창희(Chang Hee Jung),이용한(Yong Han Lee),김윤배(Yun Bae Kim) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.1
N/A Antidotal efficacy of combinational prophylactics composed of physostigmine plus procyclidine, alone or in combination with antidotes such as atropine plus 2-pralidoxime or atropine plus HI-6, was evaluated in rats. Physostigmine (0.1 mg/kg) plus procyclidine (3 mg/kg), pretreated subcutaneously 30 min prior to subcutaneous exposure to organophosphates of militarily importance, exerted protection ratios of 7.2, 6.5, 4.0, 2.9 and 8.0 fold for tabun, sarin, soman, cyclosarin and V-agent, respectively. In comparison, low effects (1.7 fold for soman and 1.3 fold for cyclosarin) were achieved with the traditional antidotes atropine (17.4 mg/ kg) plus 2-pralidoxime (30 mg/kg) administered intramuscularly immediately after organophosphate, in contrast to high effects (5.5 fold for soman and 160.0 fold for cyclosarin) with atropine (17.4 mg/kg) plus HI-6 (125 mg/kg), although the protection ratio markedly decreased when treatment of antidotes was delayed. Noteworthy, the combinational prophylactics markedly potentiated the effects of antidotes to higher than 5.0 fold in all cases. In addition, the combinational prophylactics fully prevented the seizures and excitotoxic brain injuries induced by a high dose (100 mg/kg, 1.3 LD_(50)) of soman. Taken together, it is suggested that the prophylactics composed of physostigmine and procyclidine, in combination with posttreatment antidotes, could be a promising regimen for the prevention of lethality, seizures and brain injuries induced by organophosphates possessing diverse properties.
Acetylcholinesterase에 대한 (1,3-Dioxolan-2-yl 및 Dioxan-2-yl)methylaminium 유도체의 저해 작용
피택산(Taek San Phi),김윤배(Yun Bae Kim),김지천(Jee Cheon Kim),조영(Young Cho),석대은(Dai Eun Sok),차승희(Seung Hee Cha),서원준(Won Jun Seo) 대한약학회 1994 약학회지 Vol.38 No.5
We examined the inhibitory activity of (1,3-dioxolan-2-yl and 1,3-dioxan-2-yl)methylaminium derivatives(A; 1-8) against acetylcholinesterase. Derivatives of six-membered 1,3-dioxane exhibited more potent inhibitory effect than corresponding 5-membered 1,3-dioxolanes. The presence of methyl group at C4 position of dioxane ring was effective to increase the inhibitory potency of heterocyclic analogues. The activity of N-phenacyl-aminiums was greater than that of N,N,N-trimethyl-aminiums. In general, the terminal methyl group on 1,3-dioxane ring and the phenacyl group in ammonium compound A were assumed to be important factors to enhance the inhibitory action.
유기인제 중독의 복합예방제로서의 physostigmine과 procyclidine이 랫드의 학습 및 기억에 미치는 염향
조순옥(Soon Ock Cho),박우규(Woo-Kyu Park),이선애(Sun Ae Lee),조영(Young Cho),허경행(Gyeung-Haeng Hur),김왕수(Wang-Soo Kim),천기철(Ki-Cheol Cheon),하연철(Yeon-Cheol Ha),연규백(Gyu-Baek Yeon),김지천(Jee-Cheol Kim),김형규(Hyong-Kyu Kim 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.4
The effects of physostigmine and procyclidine, as a combinational prophylactic regimen for organophosphate poisoning, on learning and memory process were examined in rats administering subcutaneously. Procyclidine at doses of 3 through 10 ㎎/㎏ produced significant impairment of learning and memory process in step-through passive avoidance test. However, this harmful effect of procyclidine (3 and 5 ㎎/㎏) was reversed by simultaneous injection of physostigmine (0.1 ㎎/㎏). Procyclidine at doses of 7 and 10 ㎎/㎏ produced reversible inhibition of learning and memory process in Morris water maze test. Physostigmine (0.1 ㎎/㎏) recovered the memorial impairment induced by procyclidine in this test. In conclusion, procyclidine can affect learning and memory process at the doses not less than 3 ㎎/㎏, and the dose of procyclidine to influence the learning and memory process in rats might be increased to more than 5 ㎎/㎏ by the simultaneous administration of physostigmine (0.1 ㎎/㎏).
유기인제 중독의 복합예방제로서의 Procyclidine과 Physostigmine의 일반약리작용
이선애(Sun Ae Lee),조순옥(Soon Ock Cho),이보영(Bo Young Lee),반주연(Ju Yeon Ban),조영(Young Cho),허경행(Gyeung-Haeng Hur),김왕수(Wang-Soo Kim),김지천(Jee-Cheon Kim),김윤배(Yun-Bae Kim),성연희(Yeon Hee Seong) 한국실험동물학회 2005 Laboratory Animal Research Vol.21 No.3
General pharmacological properties of procyclidine and physostigmine, as a combinational prophylactic regimen for organophosphate poisoning, were investigated in experimental animals and in vitro test system. Procyclidine and physostigmine were administerd by subcutaneous injection or miniosmotic pumps for sustained release. Procyclidine had no effects on general behavior, spontaneous motor activity, pentobarbital sleeping time, motor coordination and normal body temperature at the doses of less than 30 ㎎/㎏ or 1728 ㎍/㎏/hr. Gastric secretion and intestinal motility in rats were not influenced by the sustained release of procyclidine at dose of 1296 ㎍/㎏/hr. Procyclidine up to 40 ㎎/㎏ did not change the mean arterial blood pressure and heart rate in conscious rats. Procyclidine had no effect on the respiratory rate at the same doses when given to anesthetized rats. In in vitro experiments, procyclidine at the concentration of more than 0.1 and 1 ㎎/ℓ showed significant inhibitory action on the acetylcholine-and histamine-induced contractions, respectively, in the isolated ileum of guinea-pig. In the isolated guinea-pig tracheal muscle, procyclidine did not affect the histamine-induced contractions at the dose of 5 ㎎/ℓ, but showed partial inhibition on the acetylcholine-induced contractions at the doses of 3 and 5 ㎎/ℓ. Physostigmine at the concentration of 0.05 ㎎/㎏ or 36 ㎍/㎏/hr neither produced significant change of the normal physiological activity of rats, nor completely recover the inhibitory effect of procyclidine on the gastrointestinal tract. Based on these results, it was concluded that procyclidine and physostigmine did not induce marked adverse effects in experimental animals except the significant inhibitory effect on the gastrointestinal system.
Rheological Properties of Soyprotein Dope
Kim,Jee Cheon,Cho,Sook Ja,Byun,Pyung Hwa,Byun,Si Myung 서원대학교 응용과학연구소 1992 응용과학연구 Vol.1 No.1
게살과 구조적으로 유사한 대두단백 섬유는 dope를 방사하여 얻은 것으로, 대두단백의 dispersion이나 dope의 성질에 의존한다. 대두단백섬유의 출발물질인 대두단백의 dispersion과 dope의 동적 물성을 측정함으로서 그들의 점탄성을 조사하였다. 단백질 농도가 증가할때 dispersion이나 dope 모두 단백질간의 상호반응이 일어났다. Dispersion에 알칼리 용액을 넣은 dope는 고분자 중합물질에 상호연결된 특성을 나타내었다. 반면에 dispersion에서는 무정형 중합물질의 특성이 나타났다. Dispersion에 대한 화학변형이 동적물성에 미치는 영향도 관찰하였다. The dynamic rheological properties of the dope and the hydrated disperson of the soyprotein, as a starting material of soyprotein fiber, were studied to investigate their viscoelastic properties. The increase of protein concentration in the both cases of the rope cases of the dope and the dispersion resulted in the establishment of intermolecular reation of the protein. With the addition of alkali solution to the dispersion, the dope shows the characteristics of very lightly cross-linked high molecular weight polymer. In constrast, the dispersion shows the properties of an amorphorous polymer. The effects of chemical modification of the dispersion on the dynamic properties were also investigated.