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방사선의 발생독성 검색을 위한 단기 최기형성 시험법의 확립
김종춘(Jong-Choon Kim),김성호(Sung-Ho Kim),신동호(Dong-Ho Shin),신진영(Jin-Young Shin),김세라(Se-Ra Kim),이해준(Hae-June Lee),박승춘(Seung-Chun Park),조성기(Sung-Kee Jo),이윤실(Yun-Sil Lee) 한국독성학회 2004 Toxicological Research Vol.20 No.1
The present study was carried out to establish a short-term teratogenicity study for detecting developmental toxic potential induced by gamma radiation in ICR mice. Pregnant mice were exposed at dose levels of 0, 0.5, 1, 2, or 4 Gy on gestational day 8.5. All dams were subjected to caesarean section on gestational day 10.5 and their embryos were examined for growth, differentiation, and morphological abnormalities. An increase in the number of resorption was found at 4 Gy in a dose-dependent manner. Dose-dependent decreases in the developmental score of yolk sac circulation and olfactory system at above 1 Gy, in the number of somite pairs and developmental score of allantois, optic system, and maxillary process at above 2 Gy, and in the all growth and developmental parameters examined at 4 Gy were found. Various types of morphological abnormalities were seen at dose levels of 0.5 Gy or greater. Characteristic malformations induced by gamma radiation were abnormal axial rotation, hematoma, craniofacial hypoplasia, open neuropore, shortened prosencephalon, kinked somites, irregular somites, swelling, hydropericardium, absent branchial bar, and absent limb bud. Morphological alterations such as hematoma, craniofacial hypoplasia, open neuropore, and kinked somites were noted even in the lowest dose (0.5 Gy). These results indicated that the shortterm teratogenicity study established in this study can be a useful tool for not only detecting the developmental toxic potential induced by gamma radiation, but also screening radio-protective agents in ICR mice.
방사선의 발생독성 검색을 위한 단기 최기형성 시험법의 확립
김종춘(Jong-Choon Kim),김성호(Sung-Ho Kim),신동호(Dong-Ho Shin),신진영(Jin-Young Shin),김세라(Se-Ra Kim),이해준(Hae-June Lee),박승춘(Seung-Chun Park),조성기(Sung-Kee Jo),이윤실(Yun-Sil Lee) 한국독성학회 2004 Toxicological Research Vol.20 No.2
The present study was carried out to establish a short-term teratogenicity study for detecting developmental toxic potential induced by gamma radiation in ICR mice. Pregnant mice were exposed at dose levels of 0, 0.5, 1, 2, or 4 Gy on gestational day 8.5. All dams were subjected to caesarean section on gestational day 10.5 and their embryos were examined for growth, differentiation, and morphological abnormalities. An increase in the number of resorption was found at 4 Gy in a dose-dependent manner. Dose-dependent decreases in the developmental score of yolk sac<br/> circulation and olfactory system at above 1 Gy, in the number of somite pairs and developmental score of allantois, optic system, and maxillary process at above 2 Gy, and in the all growth and developmental parameters examined at 4 Gy were found. Various types of morphological abnormalities were seen at dose levels of 0.5 Gy or greater. Characteristic malformations induced by gamma radiation were abnormal axial rotation, hematoma, craniofacial hypoplasia, open neuropore, shortened prosencephalon, kinked somites, irregular somites, swelling, hydropericardium, absent branchial bar, and absent limb bud. Morphological alterations such as hematoma, craniofacial hypoplasia, open neuropore, and kinked somites were noted even in the lowest dose (0.5 Gy). These results indicated that the shortterm teratogenicity study established in this study can be a useful tool for not only detecting the developmental toxic potential induced by gamma radiation, but also screening radio-protective agents in ICR mice.
Trinitrobenzene sulfonic acid에 의해 유발된 랫드의 대장염에서 HemoHIM의 항염증 효과
이해준,김세라,문창종,김종춘,배춘식,강성수,정우희,박혜란,조성기,김성호,Lee, Hae-June,Kim, Se-Ra,Moon, Chang-Jong,Kim, Jong-Choon,Bae, Chun-Sik,Kang, Seong-Soo,Jung, U-Hee,Park, Hae-Ran,Jo, Sung-Kee,Kim, Sung-Ho 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.1
The cause and pathogenesis of inflammatory bowel disease remain unknown and no definitetherapy exists until now. The present study was conducted to investigate the anti-inflammatory effectsof a herbal preparation (HemoHIM) in colitis induced by 30 mg of trinitrobenzene sulfonic acid (TNBS)in rats. Sprague-Dawley rats were divided into 5 groups. Each group was treated with 1 mg ofHemoHIM/ml of drinking water, 4 mg of HemoHIM/ml of drinking water, 50 mg of HemoHIM/kgof body weight (i.p. once every other day) or 10 mg/kg of HemoHIMof body weight (i.p. onceevery other day) from the next day. After 2 weeks, rats were sacrificed and morphologic featuresof colons were examined. Ulceration, adhesion, thickening and dilatation were noticed in the colonicmucosa after TNBS instillation. Intraperitoneal injection of HemoHIM (50 and 100 mg/kg of bodyweight) showed the anti-inflammatory effect on adhesion, thickening, dilatation, ulceration, and theinhibition effect on damage score by 72.7% and 90.9%, respectively. Histologically, the colon of TNBS-treated rat showed inflammatory cell infiltration by polymorphonuclear cells, multiple erosive lesionsignificant improvement in these symptoms. The results obtained suggest marked anti-inflamatoryactivity of the HemoHIM at the dose levels examined.
고선량 및 저선량 방사선 조사 마우스에서 누에동충하초(Paecilomyces japonica)의 효과
김세라,오헌,이해준,신동호,김종춘,박인철,오기석,조성기,김성호,Kim, Se-ra,Oh, Heon,Lee, Hae-june,Shin, Dong-ho,Kim, Jong-choon,Park, In-chul,Oh, Ki-seok,Jo, Sung-kee,Kim, Sung-ho 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2
Cordyceps has a reputation for its broad biological activities and as a tonic which replenishs vital function in Chinese traditional medicines. As an attempt to obtain fundamental data for the development of new type Cordyceps, the effects of the fruiting bodies of cultivated fungus of Paecilomyces japonica grown on silkworm larvae on radiationinduced damages were investigated. We performed this study to determine the effect of Dongchongxiacao (Paecilomyces japonica) on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells and hair follicles of mice irradiated with high and low dose of gamma-radiation. Treatment with Dongchongxiacao showed no significant modifying effects on the jejunal crypt survival and endogenous spleen colony formation. The frequency of radiationinduced apoptosis was reduced by pretreatment of Dongchongxiacao (i.p.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.01). The spontaneous levels of apoptotic cells are $0.082{\pm}0.041$ in intestinal crypts and $0.231{\pm}0.084$ per hair follicle section of skin. Pretreatment of Dongchongxiacao was associated with decreases of 26.86% in intestinal crypt and 66.36% in hair follicle decrease in the number of cells with nuclei positively stained for apoptosis compared with the irradiation control group. We demonstrated for the first time that Dongchongxiacao administration could reduce the extent of apoptosis produced by radiation in the hair follicle. The results presented herein that Dongchongxiacao given before irradiation is capable of reducing the severity of cell loss as a result of apoptosis.
국내 자연 방사선 고준위 지역(청원군, 보은군) 사육 돼지의 림프구 미소핵 발생 평가
이해준,김창모,김세라,이진희,김중선,김종춘,김일화,김태환,류시윤,조성기,최수용,김성호,Lee, Hae-June,Kang, Chang-Mo,Kim, Se-Ra,Lee, Jin-Hee,Kim, Joong-Sun,Kim, Jong-Choon,Kim, Ill-Hwa,Kim, Tae-Hwan,Ryu, Si-Yun,Jo, Sung-Kee,Choi, Soo-Yong,K 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4
Cytogenetic and hematological analysis was performed in peripheral blood obtained from pigs bred in the high background radiation areas (HBRA) (Cheongwon-gun and Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis-blocked (CB) lymphocytes at several doses were measured in three pigs. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the swine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy. The measurements performed after irradiation showed dose-related increases in the MN frequency in each donors. The results were analyzed using a linear-quadratic model with a line of best fit of $y=0.0005404D^2+0.04237D+0.00833$ [y = number of MN/cytokinesis-blocked (CB) cells and D = irradiation dose in Gy]. MN rates per 1,000 CB lymphocytes of pig from the HBRA (Cheongwon-gun, Boeun-gun) and the control area were $6.70{\pm}2.36$, $9.00{\pm}3.50$ and $11.00{\pm}2.98$, respectively. The MN frequencies of CB lymphocytes from pigs bred in three areas means that the values are within the background variation in this experiment. The MN frequencies and hematological values were similar regardless of whether the pigs were bred in the HBRA or the control area.
자외선 B 조사 hairless 마우스에서 일광화상세포 발생 억제에 대한 녹차의 효과
김성호,김세라,이해준,이진희,김유진,김종춘,장종식,조성기,Kim, Sung-ho,Kim, Se-ra,Lee, Hae-june,Lee, Jin-hee,Kim, Yu-jin,Kim, Jong-choon,Jang, Jong-sik,Jo, Sung-kee 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.1
In this study we assessed the influences of ultraviolet (UV) light B radiation on epidermal cells by apoptotic sunburn cell (SBC) and the effect of green tea treatment on the inhibition of SBC formation in SKH1-hr mouse. The extent of changes following $200mJ/cm^2$ (0.5 mW/sec) was studied at 0, 3, 6, 12, 18, 24, 30 or 36 hours after exposure. SBCs were recognized by 3 hours after irradiation. There was tendency to increase from 3 hours to 24 hours and decrease from then to 36 hours after irradiation. The mice that received 0, 25, 50, 100, 200, 400 or $800mJ/cm^2$ of UVB were examined 24 hours after irradiation. The SBCs were induced as the radiation dose increases from 0 to $200mJ/cm^2$. A further increase of radiation dose has little further effect. The frequency of UVB ($200mJ/cm^2$)-induced SBC formation was reduced by intraperitoneal injection of green tea extract (p<0.01).
참나무 원목 및 톱밥에서 재배된 마른진흙버섯 자실체의 에탄올 추출물에 대한 항산화 및 항암활성
황미현,김영환,김길수,김태완,조우식,최성국,김종춘,박승춘,Hwang, Mi-hyun,Kim, Young-hoan,Kim, Kil-soo,Kim, Tae-wan,Jo, Woo-sik,Choi, Sung-guk,Kim, Jong-choon,Park, Seung-chun 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.2
The purpose of this study is to examine the elemental compositions, antioxidant and antitumor activity of water, 20%, 40%, 60%, and 80% ethanol extracts obtained from the fruiting body of Phellinus gilvus. In electron donating ability test, the strong activities more than 70% were observed in $80{\mu}g/ml$ of 20%, 40%, 60% and 80% ethanol extracts from the fruiting body of P. gilvus grown in oak and sawdust. The antitumor activity was evaluated by sulforhodamine B (SRB) in terms of cell survival level. The tumor cells (sarcoma 180) were treated with various ethanol extracts (water, 20, 40, 60 and 80%). The results showed that all extracts inhibited proliferation showing a dose-dependent manner against tumor cells.
돼지 림프구의 미소핵 형성을 지표로 방사선 생물학적 선량측정법 확립
김세라,이해준,이진희,강창모,김태환,조성기,김종춘,김성호,Kim, Se-ra,Lee, Hae-june,Lee, Jin-hee,Kang, Chang-mo,Kim, Tae-hwan,Jo, Sung-kee,Kim, Jong-choon,Kim, Sung-ho 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.3
The purpose of the present experiment was to investigate the micronuclei(MN) frequency in cytokinesis-blocked(CB) cells after various doses of gamma-rays in pig (Landrace, male, 3-month-old) and so to contribute to the clarification of the question whether these species are suitable as a target organism in the test system. The frequencies of binucleated cells, and gamma-ray-induced MN in CB cells at several doses were measured in three donors. The peaks of binucleated lymphocyte formation(22%) were found at a concentration of 2% phytohaemagglutinin(PHA) and $4{\mu}g/ml$ Cytochalasin B(Cyt-B) in pig at 72 hours after incubation. Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. When analysed by linear-quadratic model the line of best fit was $y=0.0183D+0.0124D^2+0.0133$(y = number of MN/CB cells and D=irradiation dose in Gy). In conclusion, the results demonstrate that it appears feasible to use pig as target organisms in the micronucleus test to estimate the cytogenetic damage caused by ionizing radiations or, potentially, chemical compounds.
자외선 B 조사 마우스에서 피부손상에 대한 보중익기탕의 효과
김중선,이해준,송명섭,서흥식,문창종,김종춘,배춘식,조성기,김성호,Kim, Joong-Sun,Lee, Hae-June,Song, Myoung-Sub,Seo, Heung-Sik,Moon, Changjong,Kim, Jong-Choon,Bae, Chun-Sik,Jo, Sung-Kee,Kim, Sung-Ho 대한수의학회 2009 大韓獸醫學會誌 Vol.49 No.1
The effect of Bu-Zhong-Yi-Qi-Tang (BZYQT) on the changes of ultraviolet (UV) light B radiation-induced apoptotic sunburn cell (SBC) and epidermal ATPase-positive dendritic cell (DC) in SKH1- hr or ICR mouse were investigated. The mice were treated with UVB (200 mJ/$cm^2$) and were sacrificed 24 h later. BZYQT (50 mg/kg of body weight) or vehicle (saline) was given i.p. at 36 and 12 h before irradiation, and 30 min after irradiation or BZYQT cream (0.2%) or cream base (vehicle) was topically treated at 24 h and 15 min before irradiation, and immediately after irradiation. The skin of SKH1-hr mouse prepared from the back of untreated mice exhibited about 0.3 SBC/cm length of epidermis, and 24 h after UV irradiation, the applied areas show an increased number of SBCs. But the frequency of UVB-induced SBC formation was reduced by intraperitoneal injection of BZYQT extract (p < 0.01). The numbers of DC in normal ICR mouse were 628.00 ${\pm}$ 51.56 or 663.20 ${\pm}$ 62.58 per $mm^2$ of ear epidermis. By 1 day after UVB treatment, the number of ATPase-positive cells/$mm^2$ were decreased by 39.0% or 27.1% in i.p. or topical application group with vehicle. Treatment of BZYQT was associated with increase of 33.9% in i.p. group (p < 0.05) or 2.7% in topical application group in the number of ATPase positive cells compared with the irradiation control group. The results presented herein that BZYQT administration could reduce the extent of skin damages produced by UVB.
생약복합조성물(HemoHIM<SUP>®</SUP>)의 항염증작용
이해준(Hae-June Lee),김세라(Se-Ra Kim),김종춘(Jong-Choon Kim),배춘식(Chun-Sik Bae),조성기(Sung-Kee Jo),정우희(Uhee Jung),박혜란(Hae-Ran Park),김성호(Sung-Ho Kim) 한국실험동물학회 2005 Laboratory Animal Research Vol.21 No.3
These studies were conducted to investigate the antiinflammatory effects of a herbal preparation (HemoHIM<SUP>®</SUP>). We used the methods of ‘carrageenan induced foot edema’, ‘inhibition test of vascular permeability’, ‘inhibition test of white blood cell migration’ and ‘formation of granuloma’ to examine the antiinflammatory effects of HemoHIM<SUP>®</SUP>. The oral administration (4 ㎎ of solids/㎖ of drinking water) or intraperitoneal injection (50 ㎎/㎏ of body weight or 200 ㎎/㎏ of body weight) of HemoHIM<SUP>®</SUP> showed antiinflammatory effect on carrageenan induced foot edema, inhibition test of vascular permeability, inhibition test of white blood cell migration and formation of granuloma. These results demonstrate a herbal preparation (HemoHIM<SUP>®</SUP>) possesses potent antiinflammatory effect.