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이해준,Lee, Hae June 한국진공학회 2014 진공 이야기 Vol.1 No.2
On this earth, we usually encounter three states of matter: solid, liquid, and gas. Those are the states we experience directly and most often in everyday life. However, if we consider the whole universe, more than 99.99 percent of our visible universe is in plasma state. The earth is in very unusual situation in the universe, and it is great virtue to whole creatures on this planet. In the beginning of the universe, however, there was only plasma. The other stuff came later after then. The Big Bang, beginning of our universe, was dominated by high-temperature plasma. It is where we all came from, and it continues to play a major role not only in our universe but also in various industrial applications of human being.
방사선 조사 마우스에서 인삼 panaxadiol계 ginsenosides의 효과에 관한 형태학적 평가
이해준,김세라,김성호,Lee, Hae-june,Kim, Se-ra,Kim, Sung-ho 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.2
The purpose of the study was to investigate the effect of ginseng saponins (panaxadiol, ginsenoside $Rb_1$, $Rb_2$, Rc, Rd) on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mice irradiated with gamma-ray. ICR mice were given each saponin (i.p. 50 mg/kg of body weight) at 24 hours before irradiation. The radioprotective effects of saponins were compared with the irradiation control respectively. The jejunal crypts were protected by pretreatment with ginsenoside Rc (p<0.05) and Rd (p<0.05). The spleen colony was increased by pretreatment with panaxadiol (p<0.05) and ginsenoside Rd (p<0.05). And the frequency of radiation induced apoptosis was significantly reduced by pretreatment with panaxadiol (p<0.05), ginsenoside Rb2 (p<0.05), Rc (p<0.05) and Rd (p<0.01). These results suggest that ginsenoside Rc, Rd might have a major radioprotective effect.
Trinitrobenzene sulfonic acid에 의해 유발된 랫드의 대장염에서 HemoHIM의 항염증 효과
이해준,김세라,문창종,김종춘,배춘식,강성수,정우희,박혜란,조성기,김성호,Lee, Hae-June,Kim, Se-Ra,Moon, Chang-Jong,Kim, Jong-Choon,Bae, Chun-Sik,Kang, Seong-Soo,Jung, U-Hee,Park, Hae-Ran,Jo, Sung-Kee,Kim, Sung-Ho 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.1
The cause and pathogenesis of inflammatory bowel disease remain unknown and no definitetherapy exists until now. The present study was conducted to investigate the anti-inflammatory effectsof a herbal preparation (HemoHIM) in colitis induced by 30 mg of trinitrobenzene sulfonic acid (TNBS)in rats. Sprague-Dawley rats were divided into 5 groups. Each group was treated with 1 mg ofHemoHIM/ml of drinking water, 4 mg of HemoHIM/ml of drinking water, 50 mg of HemoHIM/kgof body weight (i.p. once every other day) or 10 mg/kg of HemoHIMof body weight (i.p. onceevery other day) from the next day. After 2 weeks, rats were sacrificed and morphologic featuresof colons were examined. Ulceration, adhesion, thickening and dilatation were noticed in the colonicmucosa after TNBS instillation. Intraperitoneal injection of HemoHIM (50 and 100 mg/kg of bodyweight) showed the anti-inflammatory effect on adhesion, thickening, dilatation, ulceration, and theinhibition effect on damage score by 72.7% and 90.9%, respectively. Histologically, the colon of TNBS-treated rat showed inflammatory cell infiltration by polymorphonuclear cells, multiple erosive lesionsignificant improvement in these symptoms. The results obtained suggest marked anti-inflamatoryactivity of the HemoHIM at the dose levels examined.
국내 자연 방사선 고준위 지역(청원군, 보은군) 사육 돼지의 림프구 미소핵 발생 평가
이해준,김창모,김세라,이진희,김중선,김종춘,김일화,김태환,류시윤,조성기,최수용,김성호,Lee, Hae-June,Kang, Chang-Mo,Kim, Se-Ra,Lee, Jin-Hee,Kim, Joong-Sun,Kim, Jong-Choon,Kim, Ill-Hwa,Kim, Tae-Hwan,Ryu, Si-Yun,Jo, Sung-Kee,Choi, Soo-Yong,K 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4
Cytogenetic and hematological analysis was performed in peripheral blood obtained from pigs bred in the high background radiation areas (HBRA) (Cheongwon-gun and Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis-blocked (CB) lymphocytes at several doses were measured in three pigs. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the swine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy. The measurements performed after irradiation showed dose-related increases in the MN frequency in each donors. The results were analyzed using a linear-quadratic model with a line of best fit of $y=0.0005404D^2+0.04237D+0.00833$ [y = number of MN/cytokinesis-blocked (CB) cells and D = irradiation dose in Gy]. MN rates per 1,000 CB lymphocytes of pig from the HBRA (Cheongwon-gun, Boeun-gun) and the control area were $6.70{\pm}2.36$, $9.00{\pm}3.50$ and $11.00{\pm}2.98$, respectively. The MN frequencies of CB lymphocytes from pigs bred in three areas means that the values are within the background variation in this experiment. The MN frequencies and hematological values were similar regardless of whether the pigs were bred in the HBRA or the control area.
자외선 B 조사 마우스에서 피부손상에 대한 홍삼의 효과
이해준(Hae-June Lee),김세라(Se-Ra Kim),김중선(Joong-Sun Kim),문창종(Changjong Moon),김종춘(Jong-Choon Kim),배춘식(Chun-Sik Bae),장종식(Jong-Sik Jang),조성기(Sung-Kee Jo),김성호(Sung-Ho Kim) 고려인삼학회 2006 Journal of Ginseng Research Vol.30 No.4
The effects of red ginseng (RG) on the changes of ultraviolet (UV) light B radiation-induced apoptotic sunburn cell (SBC) and epidermal ATPase-positive dendritic cell (DC) in SKH1-hr or ICR mouse were investigated. The mice were treated with UVB (200 mJ/㎠) and were sacrificed 24 hours later. RG (50 mg/kg of body weight) or vehicle (saline) was given i.p. at 36 and 12 hours before irradiation, and 30 minutes after irradiation. RG cream (0.2%) or cream base (vehicle) was also topically treated at 24 hours and 15 minutes before irradiation, and immediately after irradiation. The skin of SKH1-hr mouse prepared from the back of untreated mice exhibited about 0.3 SBC/cm length of epidermis, and 24 hours after UV irradiation, the applied areas show an increased number of SBCs. But the frequency of UVBinduced SBC formation was significantly reduced by intraperitoneal injection of RG extract. The numbers of DC in normal ICR mouse were 628.00±51.56 or 663.20±62.58 per ㎟ of ear epidermis. By 1 day after UVB treatment, the number of ATPase-positive cells/㎟ were decreased by 39.0% or 27.1% in i.p. or topical application group with vehicle. The frequency of UVB (200mJ/㎠)-induced DC decrease was reduced by treatment of RG as 31.3% in i.p. group and 22.4% in topical application group compared with the irradiation control group. The results presented herein that RG administration could reduce the extent of skin damages produced by UVB.
자연방사선 고준위 지역 사육 소의 림프구 미소핵 발생 평가
이해준(Hae-June Lee),강창모(Chang-Mo Kang),김세라(Se-Ra Kim),문창종(Chang-Jong Moon),김종춘(Jong-Choon Kim),김일화(Ill-Hwa Kim),조성기(Sung-Kee Jo),장종식(Jong-Sik Jang),김성호(Sung-Ho Kim) 한국독성학회 2006 Toxicological Research Vol.22 No.4
Cytogenetic and hematological analysis was performed in peripheral blood obtained from cattle bred in the high background radiation areas (HBRA, Goesan-gun, Cheongwon-gun,Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis- blocked (CB) lymphocytes at several doses were measured in 3 cattle. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the bovine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy. The measurements performed after irradiation showed dose-related increases in the MN frequency in each donors. The results were analyzed using a linear-quadratic model with a line of best fit of y = (0.0583 ± 0.0137)D + (0.0366 ± 0.0081)D² + (0.0093 ± 0.0015) (y = number of MN/CB cells and D = irradiation dose in Gy). MN rates per 1,000 CB lymphocytes of cattle from the Goesangun, Cheongwon-gun, Boeun-gun and the control area were 6.50 ± 2.72, 9.00 ± 4.50, 10.89 ± 4.23 and 9.60 ± 4.70, respectively. The MN frequencies of CB lymphocytes from cattle bred in 4 areas mean that the values are within the background variation in this experiment. The MN frequencies and hematological values were similar regardless of whether the cattle were bred in the HBRA or the control area.
생약복합조성물(HemoHIM<SUP>®</SUP>)의 항염증작용
이해준(Hae-June Lee),김세라(Se-Ra Kim),김종춘(Jong-Choon Kim),배춘식(Chun-Sik Bae),조성기(Sung-Kee Jo),정우희(Uhee Jung),박혜란(Hae-Ran Park),김성호(Sung-Ho Kim) 한국실험동물학회 2005 Laboratory Animal Research Vol.21 No.3
These studies were conducted to investigate the antiinflammatory effects of a herbal preparation (HemoHIM<SUP>®</SUP>). We used the methods of ‘carrageenan induced foot edema’, ‘inhibition test of vascular permeability’, ‘inhibition test of white blood cell migration’ and ‘formation of granuloma’ to examine the antiinflammatory effects of HemoHIM<SUP>®</SUP>. The oral administration (4 ㎎ of solids/㎖ of drinking water) or intraperitoneal injection (50 ㎎/㎏ of body weight or 200 ㎎/㎏ of body weight) of HemoHIM<SUP>®</SUP> showed antiinflammatory effect on carrageenan induced foot edema, inhibition test of vascular permeability, inhibition test of white blood cell migration and formation of granuloma. These results demonstrate a herbal preparation (HemoHIM<SUP>®</SUP>) possesses potent antiinflammatory effect.
자외선 B를 조사한 마우스 표피멜라닌세포 변화에 대한 홍삼의 효과
이해준(Hae-June Lee),김세라(Se-Ra Kim),김중선(Joong-Sun Kim),문창종(Changjong Moon),김종춘(Jong-Choon Kim),배춘식(Chun-Sik Bae),장종식(Jong-Sik Jang),조성기(Sung-Kee Jo),김성호(Sung-Ho Kim) 고려인삼학회 2006 Journal of Ginseng Research Vol.30 No.4
We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of red ginseng (RG) on the formation, and decrease of UVB-induced epidermal melanocytes. C57BL/6 mice were irradiated by UVB 80 mJ/㎠ (0.5 mW/sec) daily for 7 days, and RG was intraperitoneally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 melanocytes/㎟, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with RG before each irradiation interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with RG at 3rd and 6th weeks after irradiation. The present study suggests the RG as inhibitor of UVB-induced pigmentation and depigmenting agent.
방사선 조사 마우스에서 학습기억 장애에 대한 홍삼의 효과
이해준(Hae June Lee),김중선(Joong Sun Kim),문창종(Changjong Moon),김종춘(Jong Choon Kim),조성기(Sung Kee Jo),장종식(Jong Sik Jang),김성호(Sung Ho Kim) 고려인삼학회 2009 Journal of Ginseng Research Vol.33 No.2
Previous studies suggest that even low-dose irradiation can lead to progressive cognitive decline and memory deficits, which implicates, in part, hippocampal dysfunction in both humans and experimental animals. In this study, whether red ginseng (RG) could attenuate memory impairment was investigated through a passive-avoidance and object recognition memory test, as well as the suppression of hippocampal neurogenesis, using the TUNEL assay and immunohistochemical detection with markers of neurogenesis (Ki-67 and doublecortin (DCX)) in adult mice treated with a relatively low-dose exposure to gamma radiation (0.5 or 2.0 Gy). RG was administered intraperitonially at a dosage of 50 ㎎/㎏ of body weight, at 36 and 12 h pre-irradiation and at 30 minutes post-irradiation, or orally at a dosage of 250 ㎎/㎏ of body weight/day for seven days before autopsy. In the passive-avoidance and object recognition memory test, the mice that were trained for one day after acute irradiation (2 Gy) showed significant memory deficits compared with the sham controls. The number of TUNEL-positive apoptotic nuclei in the dentate gyrus (DG) was increased 12 h after irradiation. In addition, the number of Ki-67- and DCX-positive cells was significantly decreased. RG treatment prior to irradiation attenuated the memory defect and blocked apoptotic death as well as a decrease in the Ki-67- and DCX-positive cells. RG may attenuate memory defect in a relatively low-dose exposure to radiation in adult mice, possibly by inhibiting the detrimental effect of irradiation on hippocampal neurogenesis.