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( Yun Jeong Jeong ),( Yongsoo Choi ),( Jae Moon Shin ),( Hyun Ji Cho ),( Jeong Han Kang ),( Hyeun Wook Park ),( Jung Yoon Choe ),( Young Seuk Bae ),( Sang Mi Han ),( Cheorl Ho Kim ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0
Bee venom is a natural compound produced by the honey bee (Apis mellifera), and has been reported as having the biological and pharmacological activities, including anti-bacterial, anti-viral and anti-inflammation. In the present study, the inhibitory effects of bee venom and its major peptide components on the tumor invasion were demonstrated. It was confirmed the inhibitory effects of bee venom, melittin, and apamin on the EGF-induced invasion of breast cancer cells. Transwell invasion and wound-healing assays showed that bee venom and melittin significantly inhibits the EGF-induced invasion and migration of breast cancer cells. Also, bee venom and melittin reduced the EGF-stimulated F-actin reorganization at the leading edge, but apamin did not affect. Particularly, melittin inhibited the EGF-induced MMP-9 expression via blocking the NF-κB and PI3K/Akt/mTOR pathway. In addition, melittin significantly suppressed the EGF-induced FAK phosphorylation through inhibition of mTOR/p70S6K/4E-BP1 pathway. These results suggest that inhibitory effects of melittin on breast cancer cell motility and migration may be related to the inhibition of mTOR pathway.ⓒ2014 Elsevier Ltd. All rights reserved.
Kim, Cheorl-Ho,Kang, Bong Seok,Kim, Yeon-Jeong,Shim, Jae-Kyoung,Song, Eun-Young,Park, Young-Guk,Lee, Young-Choon,Nam, Kyung-Soo,Kim, June-Ki,Lee, tae-Kyun,Chung, Tae-Wha The Korea Science and Technology Center 1998 BMB Reports Vol.31 No.6
In a previous paper (Kim et al., 1996a), the immediate 5'-flanking region and coding region of the human UDP-N-acetylglucosamine:-D-mannoside-1, 4-N-acetylglucosaminyltransferase III(N-acetylglucosaminyltransferase-III); GnT-III) gene was reported, isolated and analyzed. Herein, we report on amplification of a new 5'-noncoding region of the GnT-III mRNA by single-strand ligation to single-stranded cDNA-PCR (5'-RACE PCR) using poly(A)+ RNA isolated form human fetal liver cells. A cDNA clone was obtained with 5' sequences (96 bp) that diverged seven nucleotides upstream from the ATG(+1) start codon. A concensus splice junction sequence, TCTCCCGCAG, was found immediately 5' to the position where the sequences of the cDNA diverged. The result suggested the presence of an intron in the 5'-noncoding region and that the cDNA was an incompletely reverse-transcribed cDNA product derived from an mRNA containing a new noncoding exon. When mRAN expression of GnT-III in various human tissues and cancer cell lines was examined, Northern blot analysis indicated high expression levels of GnT-III in human fetal kidney and brain tissues, as well as for a number of leukemia and lymphoma cancer cell lines. Promoter activities of the 5'-flanking regions of exon 1 and the new noncoding region were measured in a human hepatoma cell line, HepG2 by luciferase assays. The 5'-flanking region of exon 1 was the most active, whilst that of exon 2 was inactive.
KIM, CHEORL HO,Lee, Young Choon,Nam, Kyung Soo,Chung, Tae Wha,Kang, Bong Seok,Kim, June Ki,Shim, Jae Kyoung,Song, Eun Young,Kim, Yeon Jeong,Park, Young Guk,Lee, Tae Kyun 생화학분자생물학회 2000 BMB Reports Vol.31 No.6
In a previous paper (Kim et al., 1996a), the immediate 5'-flanking region and coding region of the human UDP-N-acetylglucosamine:D-mannoside-l,4-Nacetylglucosaminyltransferase III (N-acetylglucosaminyltransferase-III; GnT-III) gene was reported, isolated and analyzed. Herein, we report on amplification of a new 5'-noncoding region of the GnT III mRNA by single-strand ligation to single-stranded cDNA-PCR (5'-RACE PCR) using poly(A)+ RNA isolated from human fetal liver cells. A cDNA clone was obtained with 5' sequences (96 bp) that diverged seven nucleotides upstream from the ATG (+1) start codon. A concensus splice junction sequence, TCTCCCGCAG, was found immediately 5' to the position where the sequences of the cDNA diverged. The result suggested the presence of an intron in the 5'-noncoding region and that the cDNA was an incompletely reversetranscribed cDNA product derived from an mRNA containing a new noncoding exon. When mRNA expression of GnT III in various human tissues and cancer cell lines was examined, Northern blot analysis indicated high expression levels of GnT III in human fetal kidney and brain tissues, as well as for a number of leukemia and lymphoma cancer cell lines. Promoter activities of the 5'-flanking regions of exon 1 and the new noncoding region were measured in a human hepatoma cell line, HepG2, by luciferase assays. The 5'-flanking region of exon 1 was the most active, whilst that of exon 2 was inactive.
Genetic diversity and construction of core collection in Capsicum
Hea-Young Lee,Jin-Kyung Kwon,Hee-Jin Jeong,Na Young Ro,Byoung-Cheorl Kang 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Capsicum diversity is getting lower in modern crops because of the genetic erosion. In Capsicum, breeders have been mainly focused on agriculturally important traits such as disease resistances, high yield and pungency. However, this narrow breeding pool hampered to develop improved cultivars. It has become a hot issue to conservation of genetic diversity and exploitation of wild germplasm in Capsicum. Analysis of genetic diversity and construction of core collection is the first step to make efficient use of germplasm. Although there have been several attempts to construct core collections in Capsicum, most of these works were limited due to handling small number of samples, relying mainly on the characterization of morphological traits or focusing only C. annuum species. To expand understanding of the structure and genetic diversity of germplasm in Capsicum, we need to have a highly efficient genotyping tool to handle large number of samples. Toward this end, we are analyzing 3,599 germplasm accessions including other cultivated species and wild species in Capsicum with 48 single nucleotide polymorphism (SNP) markers.
Kang, Sung-Koo,Kim, Yong-Sam,Kong, Yun-Jeong,Song, Kwon-Ho,Chang, Young-Chae,Park, Young-Guk,Ko, Jeong-Heon,Lee, Young-Choon,Kim, Cheorl-Ho WILEY-VCH Verlag 2008 Proteomics Vol.8 No.16
<P>By employing proteomics analysis tool, we examined the effects of GD3 synthase expression on the differentiation properties of chronic myelogenous leukemia (CML)-derived leukemia cells K562. Forced expression of GD3 synthase induced erythroid differentiation as determined by an increase in glycophorin A expression and synthesis of hemoglobins. The proteomic analysis revealed that 15 proteins were increased by GD3 synthase. In contrast, we observed three protein gel spots decreased in contents in the cell membranes of GD3 synthase-transfected K562 cells. Among the increased proteins, membrane transglutaminase 2 (TG2) was specifically increased in the cell membrane of GD3 synthase-transfected K562 cells. Then, we generated the GD3 synthase-transfected cells in the K562 cells. Interestingly, the TG2 level was increased in GD3 synthase-transfected cells compared with vector- and plasma membrane-associated ganglioside sialidase (Neu3)-transfected cells. In addition, its ability to be photoaffinity-labeled with [α-<SUP>32</SUP>P]GTP was also increased in the GD3 synthase- and TG2-transfected cells. Moreover, small interfering RNA (siRNA) analysis for the GD3 synthase showed the decrease or abolishment of the membrane TG2. Finally, GD3 synthase-transfected cells accelerated the erythroid differentiation. Therefore, we propose that the recruitment of TG2 into membranes by GD3 might play an important role in the erythroid differentiation in K562 cells.</P>
Jeong Ran Kim,Bong Seok Kang,Jeong Heon Ko,Jin Suk Park,Sang Jae Kim,Gil Hwan Bai,Tae Ho Chung,Kyung Soo Nam,Yong Kyung Choi,In Sung Choe,Tae Wha Chung,Young Choon Lee,Cheorl Ho Kim 생화학분자생물학회 1996 BMB Reports Vol.29 No.6
Clinical strains of Mycobacterium tuberculosis. M. terrae complex, M. gordonae. M. auium-intracellulae complex, and M. fortuitum from Korean patients were isolated and analyzed by comparing large restriction fragment (LRF) patterns produced by digestion of genomic DNA with infrequent-cutting endonucleases like Asnl and Xbal, and pulsed-field gel electrophoresis (PFGE). Three M. tuberculosis, two M. terrae complex, two M gordonae, two M auium-intracellulae complex, and two M. fortuitum strains were compared by using Asnl and Xbal, and this allowed easy visual separation of all epidemiologically unrelated strains. PFGE exhibits different DNA restriction patterns which are easy to compare. Genome size of the strains roughly ranged from 3020 to 3335 kb. The LRF patterns are useful for epidemiologic studies of tuberculosis with regard to drug resistance.