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골막기원세포에서 strontium에 의한 조골세포 표현형의 활성
김신원,김욱규,박봉욱,하영술,조희영,김정환,김덕룡,김종렬,주현호,변준호,Kim, Shin-Won,Kim, Uk-Kyu,Park, Bong-Wook,Hah, Young-Sool,Cho, Hee-Young,Kim, Jung-Hwan,Kim, Deok-Ryong,Kim, Jong-Ryoul,Joo, Hyun-Ho,Byun, June-Ho 대한악안면성형재건외과학회 2010 Maxillofacial Plastic Reconstructive Surgery Vol.32 No.3
This study investigated the effects of strontium on osteoblastic phenotypes of cultured human periostealderived cells. Periosteal tissues were harvested from mandible during surgical extraction of lower impacted third molar. Periosteal-derived cells were introduced into cell culture. After passage 3, the periostealderived cells were further cultured for 28 days in an osteogenic induction DMEM medium supplemented with fetal bovine serum, ascorbic acid 2-phosphate, dexamethasone and at a density of $3{\times}10^4$ cells/well in a 6-well plate. In this culture medium, strontium at different concentrations (1, 5, 10, and 100 ${\mu}g$/mL) was added. The medium was changed every 3 days during the incubation period. We examined the cellular proliferation, histochemical detection and biochemical measurements of alkaline phosphatase (ALP), the RT-PCR analysis for ALP and osteocalcin, and von Kossa staining and calcium contents in the periostealderived cells. Cell proliferation was not associated with the addition of strontium in periosteal-derived cells. The ALP activity in the periosteal-derived cells was higher in 5, 10, and 100 ${\mu}g$/ml strontium-treated cells than in untreated cells at day 14 of culture. Among the strontium-treated cells, the ALP activity was appreciably higher in 100 ${\mu}g$/ml strontium-treated cells than in 5 and 10 ${\mu}g$/ml strontium-treated cells. The levels of ALP and osteocalcin mRNA in the periosteal-derived cells was also higher in strontium-treated cells than in untreated cells at day 14 of culture. Their levels were increased in a dose-dependent manner. Von Kossa-positive mineralization nodules were strongly observed in the 1 ${\mu}g$/ml strontium-treated cells at day 21 and 28 of culture. The calcium content in the periosteal-derived cells was also higher in 1 ${\mu}g$/ml strontium-treated cells at day 28 of culture. These results suggest that low concentration of strontium stimulates the osteoblastic phenotypes of more differentiated periosteal-derived cells, whereas high concentration of strontium stimulates the osteoblastic phenotypes of less differentiated periosteal-derived cells. The effects of strontium on osteoblastic phenotypes of periosteal-derived cells appear to be associated with differentiation-extent.
김신원,허준,박율진,Kim, Shin-Won,Heo, Jun,Park, Yool-Jin 한국조경학회 2000 韓國造景學會誌 Vol.27 No.5
The purpose of this study is to provide the basic data for planning and designing Village Parks in Seoul in the future, which result from analyzing post-occupancy evaluation. For conducting this study, 10 Village Parks among 45, built between 1996 and 1997, were selected and analyzed. The results of this study are as follows. The degree of satisfaction of 10 Village Parks in Seoul has been evaluated on a scale of 10, to be a 6.68 average. This result means that the most respondents are satisfied with the existing Village Parks in Seoul. The correlation of the degree of satisfaction with major planning factors for 3 types of Village Parks shows that accessibility is the most important factor. As for future directions of planning and designing Village Parks, many kinds of sitting, leaning and resting place need to be provided. It is also necessary to improve the visual quality of the parks with various spatial elements. The research also shows that various facilities for people's use should be provided in Village Parks.
형태주제 분석을 통한 마을마당의 설계특성 -서울시의 사례-
김신원,허준,Kim, Shin-Won,Heo, Jun 한국조경학회 2000 韓國造景學會誌 Vol.28 No.2
In this study, design characteristics of Village Parks in Seoul are investigated through an analysis of structuring themes expressed in spatial design. The goals and objectives of this study are: to emphasize the significance of design themes; and to demonstrate an approach to the design of landscapes through an analysis of structuring themes. For conducting this study, geometric forms and naturalistic forms were examined first. That is, the following 15 structuring themes were examined, as guiding themes: the 90$^{\circ}$rectangular theme; the 45$^{\circ}$/90$^{\circ}$angular theme; the 30$^{\circ}$/60$^{\circ}$ angular theme; circles on circle; concentric circles and radii; arcs and tangents; circle segments; the ellipse; the spiral; the meander; the free ellipse and scallops; the free spiral; the irregular polygon; the organic edge; and clustering and fragmentation. Forty five Village Parks in Seoul, built between 1996 and 1997, were analyzed through these 15 structuring themes. An analysis of Village Parks was conducted by the following two categories: land shape and structuring themes; and design directions and structuring themes. The research results are as follows; 1) Geometric forms are more frequently applied than naturalistic forms. 2) Regardless of land shape, geometric forms derived from simple primary shapes, the square and the circle, are frequently used. And the meander and the irregular polygon among naturalistic forms are frequently used. 3) Regarding design directions and structuring themes, design concepts, symbolic meanings and spatial forms are, to some degree, integrated. 4) The spiral is not applied among geometric forms. And the meander and the irregular polygon, as naturalistic forms, are frequently utilized. Research findings obtained from this study could be used in the designing of future Village Parks. For a profound study, future, research is needed in two-dimensional plans and three-dimensional elements of Village Parks.