한우(韓牛)의 위내(胃內)에 서식(棲息)하는 섬모충(纖毛蟲)의 동태(動態)

이재구,백병걸,김수명,Rhee, Jae Ku,Baek, Byeong Kirl,Kim, Su Myung 대한수의학회 1975 大韓獸醫學會誌 Vol.15 No.2

In order to observe the kinetic of ciliates inhabited in the stomach, investigation of the content juice in the stomach was carried out on the 30 Korean native cattle, selected as healthy ones and slaughtered at Jeonju abattoir in August, 1974. The following is a brief summary of the leading facts gained through the experiment. 1. The average pH of the content juice showed $6.567{\pm}0.056$(SE) in the rumen, $6.607{\pm}0.053$ in the reticulum, $6.183{\pm}0.056$ in the omasum and $2.627{\pm}0.061$ in the abomasum. 2. The presumed number of ciliates per ml was calculated $168,200{\pm}17,035$(SE) in the rumen, $173,200{\pm}19,728$ in the reticulum, $23.600{\pm}3.537$ in the omasum and $467{\pm}266$ in the abomasum. 3. In the content juice the number of ciliates was gradually slowed down, the pH decreased from rumen to abomasum and the changes were denoted by quadratic curves(parabolas). 4. In accordance with the increase of the number of ciliates the pall also becomes higher and in this situation the correlation coefficient was ${\gamma}_{xy}=0.508$. 5. Population density of ciliates was checked and Entodinium showed 60 per cent among them, but Polyplastron was figured only from 0 to 0.05 per cent. 6. Among 26 species of ciliates which were identified from the content juice, Entodinium simplex was showed the highest population density, raging from 40 to 50 per cent, Dasytricha ruminantium was neat to it, Eudiplodinium affine after the next, and Entodinium caudatum and Eudiplodinium maggi were also found abundantly in the abomasum.

소시지 및 어단의 형태학적 분석

이재구(Jae Ku Rhee),김수명(Su Myung Kim),최선남(Sun Nam Choe) 전북대학교 농업과학기술연구소 1976 농업생명과학연구 Vol.7 No.-

석고가미복합방이 Anti-CD40과 rIL-4로 유도된 생쥐의 B 세포에서 싸이토카인 생성 및 면역글로블린 E에 미치는 효과

최문석 ( Moon Suk Choi ),김수명 ( Su Myung Kim ),남궁욱 ( Uk Namgung ),김동희 ( Dong Hee Kim ) 대전대학교 한의학연구소 2004 혜화의학회지 Vol.13 No.2

청열해독복합방의 항종양작용에 대한 실험적 연구

진가현 ( Ga Hyun Jin ),김수명 ( Su Myung Kim ),강탁림 ( Tak Lim Kang ),김동희 ( Dong Hee Kim ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.1

N/A Objectives : To evaluate the antitumor activity and antimetastatic effects of Cheongyoelhaedokbang(CHB), studies were done experimentally. Method : The study was carried out to evaluate antitumor activity, cytotoxicity, topoisomerase 1 activity, apoptosis and T/C% were measured, and also pulmonary colonization and histological changes of lung in B16-F10 injceted mice, levels of blood vessel formation in the CAM assay and platelet coagulation were measured to prove antimetastatic effects. Results : The results were obtained as follows 1. Treatment of CHB showed cytotoxic effect on A549, SK-OV-3, NIH: OVCAR-3, MCF-7 and MDA-MB-231 cancer cell line by 40% or higher than non-treated control cells at conc. of 100 ㎍/ml. 2. IC_50 value for the inhibition of topoisomerase 1 enzyme activity by CHB was 50-100 ㎍/ml. 3. Treatment of Jurkat cells with CHB and FACS analysis didn`t showed significant changes compared to non-treated control cells. 4. The T/C% was 127.65% in treatment of CHB in S-180 hearing ICR mice. 5. CHB treatment inhibited the adhesion of A549, SK-OV-3 cancer cell line to the complex substrates of culture dish surface. Cell detachment with CHB treatment (100 ㎍/ml) was higher than 40% compared to non-treated control cells. 6. In pulmonary colonization assay, a number of colonies in the lungs were decreased significantly in treatment of CHB compared to non-treated control cells. 7. In the histological changes of lung in B16-F10 injected mice, infiltration area of cancer cells were inhibited effectively in CHB treated group and also infiltration of lymphocytes were shown in pulmonary vein of CHB treated group. 8. In the CAM assay by which the levels of blood vessel formation can be determined quantitatively, CHB treatment inhibited vessel formation by 50%. 9. CHB treatment (10 ㎍/ml or higher concentrations) completely inhibited platelet coagulation by ADP. And also inhibited platelet coagulation by epinephrine in dose dependent manner. Conclusion : From above results it was concluded that CHB could be usefully applied for the prevention and treatment of cancer. and it is still necessary to research on synergic effect with key prescreptions in relation to cancer therapy in oriental medicine

독맥경과 임맥경에 대한 심층해부학

김수명 대전대학교 한의학연구소 2004 혜화의학회지 Vol.12 No.2

For the purpose of experimental and clinical studies on the 14 channels and collaterals, we performed an inquiry on the anatomical mapping of neronal, circulatory and muscular networks in the human body which possibly correspond to the major pathway of Du channel and Ren channel known to be critical for overall controlling and mediating 12 regular channel connections. The theoretical background of this analysis was based on the oriental medicinal treatises which described acu-points and related anatomical informations. We suggest that rearrangement methodology for the 12 regular channels as described here may be applied to other 12 regular channels and to develop the new concept on the elucidation of the medical efficacy based on precise anatomical localization of channels and collaterals as well as the meridian points. It is proposed that interdisciplinary studies between oriental and western medicine are critical for more efficient achievement.

MSG 투여가 초생추 갑상선에 미치는 영향

김수명 최신의학사 1984 最新醫學 Vol.27 No.7

만화가 아동화에 미치는 영향

김수명 대구교육대학교 1968 論文集 Vol.4 No.-

Caricature shops, poorly provided and injurious to health, are on the increase in every nook and corner of the street. Young boys and girls are absorbed in reading unsound caricatures there. It is said that, educationally, caricature is meager in contents and liable to check the development of education. Especially, the caricatures exercise a bad influence upon the children, s drawings - conceptional, imitative and not creative in technique or coloring. This is far from the original object of the fine arts education. Therefore, efforts must be urgently made to make an emotional, mental and educational examination of the contents of the existing caricatures and provide the children with sound and interesting ones.

韓國人의 內臟神經에 대한 形態學的 硏究

김수명,최창도 한양대학교 의과대학 1982 한양의대 학술지 Vol.2 No.1

The splanchnic nerves have various origins and the patterns in their formation. Edwards and Baker(1940) reported that the most frequent origins of the greater splanchnic nerves were 8th and 9th thoracic sympathetic ganglia and the highest percent of greater splanchnic nerves arose from only 3 ganglia. He also emphasized the need for a more careful consideration in variability of patterns of the splanchnic nerves. Reed(1951) had determined the origins and sources of the greater splanchnic nerves varied in 58 patterns. The purpose of this investigation is to observe the variations of the origins and the patterns of the splanchnic nerves in 65 cadavers in Korean. The results of the study are as follows: 1. The greater splanchnic nerve was present in 100% of the cases, the lesser splanchnic nerve in 96.2%, and the lowest splanchnic nerve in 46.9%. 2. The greater splanchnic nerves revealed the variations in origins from the 4th to the 11th thoracic sympathetic ganglia, the lesser splanchnic nerves from the 8th to the 12th, and the lowest splanchnic nerves from the 10th to the 12th thoracic ganglia. 3. The most frequent orgins of the greater splanchnic nerves were 3 ganglia from the 7th (80.8%), the 8th (80.0%) and the 9th (74.6%) thoracic sympathetic ganglia; of the lesser were 2 ganglia from the 10th (57.6%) and the 11th (67.2%); and of the lowest was 1 ganglion from the 12th ganglion (83.6%).

유기제인 농약(EPN)이 Mouse 의 간장에 미치는 영향

尹希炳,金壽明,李圭植 漢陽大學校 環境科學硏究所 1982 環境科學論文集 Vol.3 No.-

유기인제 살충제는 농촌에서는 물론 도시에서도 살충제로 널리 사용되고 있으며 곤충에는 독성이 강하지만 사람이나 다른 포유동물에는 비교적 독성이 약하게 작용하는 것으로 알려져 있다. 그러나 근래에 이르러 농작물에 대한 병충해 방지를 위한 유기인제 살충제의 사용빈도가 급증함에 따라 그 취급과 부주의로 인한 인체에 대한 중독작용이 빈번하게 발생되여 사회문제로 대두되고 있다. 유기인제계 화합물은 화학무기용 독 gas 를 발견해 내려는 연구중에 부산물로 합성 생성된 것이며 EPN 은 tetraethyl pyrophosphate (TEPP), parathion, methylparathion, chlorthion, diazinon, sumithion, dipterex 및 dichlorovos (dimethyl-2, 2-dichlorovinyl phosphate, DDVP)와 같은 유기인제 살충제의 화학적 유도체이다. 유기인제 살충제중 ethoxy-p-nitrophenoxyphenylphosphine sulfide(EPN)은 비교적 속히 가수분해되고 생체내에 축적작용도 적다고 하나 다량의 EPN 을 흡수하였을 때에는 급성중독이 발생된다고 알려져 있다. 유기인제 살충제의 취급부주의로 복용한 malathion 중독환자가 호흡곤란, 저혈압 및 의식불명을 일으키며 또한 밀폐된 온실에서 parathion을 살포한 노동자가 급성중독을 나타내어 사지마비와 현기증을 일으켰으며 2년 후에는 완전한 근육마비를 일으켜 보행곤란, 기억감퇴, 집중력상실과 정력감퇴가 야기되었다고 보고되었으며 이상과 같이 유기인제 살충제로 인하여 중독되었을 때에는 신경계와 호흡계를 비롯한 여러 기관에 손상이 야기되고 적절한 치료를 받지 못하여 사망한 예도 보고되었다. 저자는 유기인제 살충제로 널리 사용되는 EPN이 다른 간독성 화합물과 같이 간장에서 해독과정을 거칠 때에 간실질세포에 독성으로 작용할 것으로 사료되어 mouse에 EPN을 투여하고 시간경과에 따라 나타나는 간장의 형태학적 변화를 조직학적으로 추구하였다. 실험동물은 체중 20gm 내외의 웅성 mouse에 EPN을 olive 유에 1000배로 희석하여 0.6ml을 복강내로 주사하고 6시간, 12시간, 24시간 및 36시간 경과 후에 경동맥사혈로 희생시키고 개복한 다음 간장을 적출하였다. 적출한 일부 간조직은 10% 중성 formalin 용액에 고정하여 hematoxylin 및 eosin 염색을 하여 간장의 형태학적 변화를 관찰하고 또한 oil red O 염색을 하여 간실질세포내 지질의 축적을 관찰한 바 다음과 같은 결과를 얻었다. 1. EPN을 투여한 mouse의 간장에서 6시간 경과 후에는 간소엽 중심대와 중간대의 간실질세포내에서 경한 세포종창과 소포가 형성되었으나 24시간 후에는 간소엽 전역의 간실질세포에서 심한 세포종창과 소포가 형성되었으며, 36시간 경과 후에는 미약한 세포종창과 약간의 소포가 관찰되었다. 2. EPN을 투여한 mouse의 간장에서 6시간 경과 후에는 간소엽 중심대와 중간대의 간실질세포에서 소수의 지방소적이 형성되었으며 24시간 경과 후에는 많은 지방소적이 형성되었고 36시간 경과 후에는 약간의 지방침착이 관찰되었다. 이상 소견을 종합 분석한 바 EPN은 초기에는 mouse 간장의 간소엽 중심대와 중간대의 간실질세포의 공포변화와 지방침착을 야기하나 시간이 경과됨에 따라 점차 회복된다고 사료된다. Organophosphate insecticides have increased the yield of agricultural produce and have controlled vectors of some epidemic diseases. Organophosphates are relatively rapidly hydrolyzed and hence have less cumulative and ecologic effects. However, they have acute toxic effects and have caused numerous fatalities in man and domestic animals. It is reported that patients with ethoxy-p-nitrophenoxyphenylphosphine sulfide(EPN), which is an organophosphorus compound, has induced acute or chronic intoxications mainly due to the accumulation of acetylcholine at the cholinergic synapses and may be classified as a compound acting muscarinic (parasympathetic), nicotinic (sympathetic and motor) and central nervous system manifestations according to the site of action. The author has demonstrated the effect of EPN on the liver of mice, observing the morphological changes and accumulation of fat in the liver of the mice treated with EPN. The experimental animals were given 0.6 ml of EPN diluted in 1000 times with olive oil by intraperitoneal injection. The animals of the experimental group was killed at the intervals of 6, 12, 24 and 36 hours after administration of EPN, respectively. The histological preparation were stained with hematoxylin and eosin method for histological changes of the liver and oil red O stain for fat deposits in the hepatic parenchymal cells of the EPN treated mice. The results were as follows; 1. It is observed the cellular swelling accompanied by the vesicular formation in the hepatic parenchymal cells of central and intermediate zone of the hepatic lobule at the 6, 12 and 24 hours after administration of EPN. 2. It is observed fat deposits in the hepatic parenchymal cells of central and intermediate zones of the hepatic lobule at the 6, 12 and 24 hours after administration of EPN. 3. These was no significant histological change in the hepatic parenchymal cell of the liver at 36 hours after administration of EPN. The above findings may suggest that EPN would induce cellulars swelling associated with vesicular degeneration and fatty degeneration of the hepatic parenchymal cells at the central and intermediate zones of the hepatic lobule in albino mice.

소시지 및 魚團의 形態學籍 分析

李宰求,金壽明,崔善男 전북대학교 농업과학기술연구소 1976 農大論文集 Vol.7 No.-

Eight kinds of sausage, six fish ball cans and a fish paste were collected from the market-places in1975 and the same frozen sections were prepared at -25℃, stained with coincident PAS, sudan H ,carbol·thionine and light-green. Number three fish ball can was stained also with hematoxylin-eosin by means of the paraffin section. The results of their morphological analysis were summarized as follows: 1. With the exception of fish paste, protein contents were generally at low level ; sausage 3.82~9.41 % and fish ball 2.72~8.97 %. 2. The fat contents depended on the kind of fish ball cans, ranging from 1.41 to 17.57 %. The sausage had tremendous fat contents. but the fish paste included no fat at all. 3. Sausage and fish ball contained the largest amount of carbohydrate : sausage, 55.36∼72.34 % ;fish ball, 58.13∼77.08 %; but the fish paste, only 23.84 %. 4. Generally a large quantity of air bubble was contained: from 12.08 to 21.14% 5. In general, intermixture of the three nutrients was not moderate. Number one, two, five, six and seven of sausage and number six of fish ball showed intermixture of the fat in muscles. In the other cases, muscles mainly consisted of protein. 6. In general, intermixture of spice was moderate except for the fish ball of number three. In case of number three fish ball and number six sausage, numerous fragments of cartila rod of the gill bar were found. 7. In number six fish ball, the microorganisms were observed around the degenerated protein, carbohydrate and air bubbles, even in fresh can. But when the sausase and the other fish ball cans were kept for four dars at 37℃, bacterial colony was formed largely around the degenerated carbohydrate, protein and air butbble. In the decomposed tissues with severe damages, the unstainedzones were in the vicinity of locus.