어머니의 정서표현과 정서표현수용태도가 아동의 자기조절능력에 미치는 영향

김문신,김광웅 한국놀이치료학회 2003 한국놀이치료학회지(놀이치료연구) Vol.6 No.1

The purpose of this study is to find out how mother's emotional expressiveness and maternal attitudes toward children's expressiveness affect on the children's self-regulation. The major results of this study are as follows. First, There were differences in mother's emotional expressiveness and children's self-regulation b?'the mother?'s educational background. Children's self-regulation was also differed by a monthly income. Second, children's self-regulation are affecting by mother's negative emotional expressiveness and maternal attitude children's expressiveness The more mother's negative emotional expressiveness was frequently and the more maternal attitude toward girl's expressiveness was controlling, girl's self-regulation was low. Third, children's self-regulation was differed by the level of mother's negative emotional expressiveness and the level of maternal attitude toward children's expressiveness. The level of mother's negative emotional expressiveness was lower and the level of maternal attitude children's expressiveness was higher, children's self-regulation tend to be higher.

상피성 난소암에 관한 임상 및 병리학적 고찰

김문신,황일천,박종설,김진국,김두상 대한산부인과학회 1984 Obstetrics & Gynecology Science Vol.27 No.4

내소암에 대한 second-Look ope ration 4예

김문신,김두상 대한산부인과학회 1982 대한산부인과학회 학술대회 Vol.49 No.-

흰쥐 자궁의 Adrenoceptor에 관한 연구

김문신 한양대학교 의과대학 1982 한양의대 학술지 Vol.2 No.2

1. Effects of several drugs (adrenergic, cholinergic & prostaglandins) and field stimulation of the isolated rat uterus were investigated in extrus and nonestrus (di-and proestrus) stage. Regular and potent spontaneous motilities were observed in the nonestrus, while irregular and weak spontaneous motilities were observed in the estrus. 2. Acetylcholine and methacholine contracted all uterus and the contraction was inhibited by atropine. 3. The responses of the nonestrus to norepinephrine (NE) and methoxamine (ME), α₁-adrenoceptor agonists, differed from preparation to preparation. Some showd contraction, while others showed inhibition. The contractile responses were reduced by thymoxamine and phentolamine but not affected by yohimbine, an α₂-adrenoceptor antagonist. The inhibitory responses were antagonized by propranolol. 4. The responses of the estrus to NE and ME and were inhibitory. Isoproterenol (ISP) inhibited the motility in both stages. These inhibitions including the inhibition by NE and ME were all decreased in the presence of propranolo. Clonidine, α₂-adren-oceptor agonist, did not cause any responses. 5. Transient contractile responses were caused by field stimulation in all preparations. The contraction was markedly inhibited by tetrodotoxin, atropine and indomethacin, and increased by physostigmine and PGF₂α. The contraction, however,was not affected by clonidine, yohimbine, and thymoxamine. 6. The above results suggest that excitatory α₁-adrenoceptors and inhibitory β-adrenoceptors exist in the uterus, and that endogenous A Ch and prostaglandins seem to participate in the contractile response to field stimulation.

ICR종 생쥐의 간에서 얻은 δ - Aminolevulinate Dehydratase 동위효소의 생화학적 성상

김문신,정규철 ( Moon Shin Kim,Kyou Chull Chung ) 생화학분자생물학회 1979 BMB Reports Vol.12 No.4

Two different fractions with δ-aminolevulinate dehydratase activity designated isozyme I and I[ tentatively were isolated from liver homogenate of ICR inbred mice by slightly modifying the procedure of Doyle and Schimke, and further their physicochemical properties were characterized as follows. 1. Optimal activities of the isozyme I and II appeared to be pH 6.5 in phosphate buffer. However, catalytic activities of the both isozymes were shown to be more active in Tris-HCl buffer at pH 7.5 than in the phosphate buffer at the same pH. 2. Both of the isozymes were found to be relatively heat stable, of which stability of the isozyme I was more resistant comparable to isozyme II. 3. Apparent Michaelis-Menten Constants of the both isozyme I and II in the present experiment were 2.1 × 10^(-4)M and 5.5 × 10^(-4)M, respectively. 4. Inhibition of the enzyme activity by heavy metals such as Pb^(++) and Hg^(++)+ in vitro was more debiliated in isozyme II than in isozyme I. 5. It was found that isozyme I could not be isolated by the same procedure as was done in the normal group from lead nitrate-treated group. In addition, the enzymatic activity of isozyme II was also decreased remarkably by the same treatment. The animals used have been treated by injecting intraperitoneally 1% lead nitrate solution with 1.3 ml/100 gm of body weight to each mouse 3 weeks prior to performing the experiment. 6. Both the isozyme I and II showed the different electrophoretic mobilities on immunoelectrophoresis. Rabbit anti-sera to isozyme II have been applied.

ICR 종 생쥐의 간에서 얻은 $\delta$-Aminolevulinate Dehydratase 동위효소의 생화학적 성상

김문신,정규철,Kim, Moon-Shin,Chung, Kyou-Chull 생화학분자생물학회 1979 한국생화학회지 Vol.12 No.4

ICR 종 친근교배계의 성숙한 생쥐 125마리에서 적출한 128.8gm의 간에서 Doyle and Schimke(1969)의 방법의 변법으로 $\delta$-ALAD를 분리정제하였던 바 분자량이 다른 2개의 효소분획을 얻었다(이 영원과 정 규철, 1979). 이 효소분획 I과 효소분획 II의 물리화학적, 효소화학적 및 면적화학적 성상을 조사하여 다음과 같은 결과를 얻었다. 1. Potassium phosphate 완충액에서의 두 효소분획의 최적 pH는 6.5로서 서로 같았으며, pH가 6.5이상으로 됨에 띠라 효소활성치는 계속 감소하였다. 그러나 pH7.5에서는 potassium phosphate 완충액을 사용할 때보다 Tris 완충액을 사용할 때 그 활성치가 높게 나타났다. 2. $\delta$-ALAD는 열에 대한 안정성이 비교적 강한 효소임을 알 수 있으며, 특히 효소분획 I의 안정성이 효소분획 II의 안정성보다 강하였다. 3. Michaelis-Menten 상수는 효소분획 I이 $2.1{\times}10^{-4}M$이고, 효소분획 II의 Km 값인 $5.5{\times}10^{-4}M$의 1/2.6이었다. 4. $Pb^{++}$ 및 $Hg^{++}$ 등 중금속에 의한 시험관내에서의 효소활성의 억제착용은 효소분획 I 보다는 효소분획 II에서 현저하였다. 5.1% lead nitrate 용액을 체중 100gm 당 1.3ml씩 생쥐의 복강내에 주사하고 3주 후에 간의 $\delta$-ALAD를 정제하였던 바 효소분획 I의 활성치는 완전히 없어졌고 효소분획 II의 활성치도 현저하게 감소하였다. 6. 효소분획 II에 특이한 토끼의 항혈청에 대한 효소분획 I과 효소분획 II의 면역화학적 반응을 보면 두 효소품획의 면역침전물의 이동양상은 서로 상이하였다. 이상의 연구 결과로 미루어 ICR종 생쥐의 $\delta$-ALAD에는 두 개의 동위효소가 있음을 알 수 있다. Two different fractions with $\delta$-aminolevulinate dehydratase activity designated isozyme I and II tentatively were isolated from liver homogenate of ICR inbred mice by slightly modifying the procedure of Doyle and Schimke, and further their physicochemical properties were characterized as follows. 1. Optimal activities of the isozyme I and II appeared to be pH 6.5 in phosphate buffer. However, catalytic activities of the both isozymes were shown to be more active in Tris-HCl buffer at pH 7.5 than in the phosphate buffer at the same pH. 2. Both of the isozymes were found to be relatively heat stable, of which stability of the isozyme I was more resistant comparable to isozyme II. 3. Apparent Michaelis-Menten Constants of the both isozyme I and II in the present experiment were $2.1{\times}10^-4M$ and $5.5{\times}10^-4M$, respectively. 4. Inhibition of the enzyme activity by heavy metals such as $Pb^{++}$ and $Hg^{++}$ in vitro was more debiliated in isozyme II than in isozyme I. 5. It was found that isozyme I could not be isolated by the same procedure as was done in the normal group from lead nitrate-treated group. In addition, the enzymatic activity of isozyme II was also decreased remarkably by the same treatment. The animals used have been treated by injecting intraperitoneally 1% lead nitrate solution with 1.3 ml/100 gm of body weight to each mouse 3 weeks prior to performing the experiment. 6. Both the isozyme I and II showed the different electrophoretic mobilities on immunoelectrophoresis. Rabbit anti-sera to isozyme II have been applied.

正常人의 血淸 Ribonuclease 性狀에 대한 硏究

李源甲,高在景,金汶信 한양대학교 의과대학 1982 한양의대 학술지 Vol.2 No.2

In the present study, activities of ribonucleases (RNase) present in normal human serum were determined at acidic (pH 6.0) and neutral (pH7.5) media using polyribonucleotides as substrate. The RNases in normal human serum were separated by DEAE-cellulose chromato-graphy and their properties were partially characterized. Both acid and neutral Rnases in normal human serum exhibited the highest activity against polycytidylate (poly C) and the lowest activity against purine ribonucleotides, the decreasing order of the activity being that measured with poly C, polyuridylate, RNA, polyadenylate and polyguanylate, respectively. These results indicated that the RNases in normal human serum showed grater affrinity to the phosphodiester bonds involving pyrimidine bases especially cytosine. DEAE-cellulose chromatographical analysis for the RNases revealed that normal human serum did contain at least three types of RNases: Rnases active against both Rna and poly C; RNase active against RNA; RNase active against poly C.

전치 태반에 관한 임상적 고찰

김문신(MS Kim),강준호(JH Kang),김학성(hS Kim),김두상(DS Kim) 대한산부인과학회 1977 Obstetrics & Gynecology Science Vol.20 No.5

1972년 1월부터 1975년 6월까지 42개월간에 중앙의대부속 한강성심병원 산부인과에 입원분만된 3,310예중예 76예의 전치태반(발생빈도 44:1)을 경험하여 다음과 같이 결론한다. 1. 전치태반의 발생빈도는 기타 발표처럼 초산부보다 경산부에 많았고 특히 연령이 증가될수록 고율화되어 갔다. 2. 전치태반의 발생 바로전의 임신의 처리를 조사한바 소파 및 기타 자궁내손상력이 61. 9% 있었는바 이러한 조작이 자궁강내에 해부조직학적 변화를 초래하여 저자의 전치태반 고율발생의 원인이 된 것으로 추측된다. 3. 전치태반의 종류를 전 전치태반 27예 부분전치태반 36예, 하방부착 13예였다. 이중 12예는 유착태반이 합병되어 있었다. 4. 전치태반때의 출혈은 대량출혈 57예, 중등량 4예, 소량 14예, 무출혈 1예 였으며, 출혈의 시작은 임신 35주 이하 51예, 36주 이상 25예였다. 5. 출혈시 동통예가 49예(61.5%), 규칙적 및 불규칙적 동통을 호소한 예가 24예(31.6%) 였다. 6. 태위이상은 임신 39주 이상 28예중 10예(35.7%) 38주 미만 48예중 40예(83.4%)였다. 7. 임상병리검사의 결과는 분만전 헤모글로빈치가 10gm/dl미만 37예, 그 이상 39예였 으며, 체온은 36°9`이하 54예, 37°- 37°9` 가 22예였다. 8. 전치태반 진단은 임상증상과 태반촉지에의한 것 58예, 임상증상과 태반촉지 및 태반 조영술에 의한 것 7예, 임상증상과 태반조영술에 의한 것 5예, 기타질환의 진단하에 제왕절개술중 발견된 것 3예, 질식분만후 용수태반박리시에 진단된 것 3예였다. 9. 분만방식은 하횡절개 27예(35.5%), 종절개 22예(29%), 제왕자궁전전술 11예(14.5%), 질식분만 16예(21%)였다. 10. 전치태반 치료에 있어서 파막의 효과를 분석한바 이를 실시한 13예중 출혈억제효과는 전예에서 발휘되었으며 이중 10예는 질식분만을, 3예는 제왕절개술에 의했다. 질식분만 10예중 2예에 있어서는 초임부 였다. 11. 주산기 사망율은 태아체중 2500gm 이상일 때에 11.4%, 임신 36주 이상일 때에 11. 9%로서 각각 그 이하군보다 월등한 저율이었고, 전전치태반일 때는 기타 종류보다 3배 이상의 고율이었다. 12. 전치태반에 대한 대기요법은 단 1예에 대하여 성공, 실시되었으나 저자의 전술한 결과로 보아 가능하면 임신 36주까지 입원, 대기함이 현명함을 알았다. 76 cases of placenta previa out of 3,310 deliveries were experienced during the period of 42 months from January, 1972 to june, 1975 in the Dept. of Obst. and Gynec. Han Gang Sung Sim Hospital, Chung-Ang University with the following results; 1. The incidence of placenta previa was more frequent in multiparas than in primiparas as in the other reports increasing highly as the age advances. 2. Evaluation of the management for the preceding pregnancy revealed that the history of curettage and the other injurious processes to the endometrial surface, was available in 61.9%. These processes were supposed to incur anatomical changes to the endometral surface, accounting for the high incidence of placenta previa in the author`s and cases. 3. The varieties of placenta previa incluede 27 cases of totales, 36 cases of the partialis and 13 cases of low-lying placenta. In 12 cases of these, planta previa in the author`s and cases. 4. The amount of hemorrhage in placenta previa was massive in 57 cases, moderate in 4 cases, scanty in 14 cases, and none in 1 case respectively. 51 cases among these took the onset of hemorrhage before 35 weeks of gestation, 25 cases after 36 weeks. 5. The hemorrhage accompanied no pain in 49 cases(64.5%), but regular or irregular pain in 24 cases(31.6%). 6. Abnormal presentation were noted in 10 of 28 cases over 39 weeks of gestation and in 40 of 48 cases below 38 weeks of gestation. 7. The value of hemoglobin checked antepartum, was less than 10 gm/dl in 39 cases. Body temperature was lower than 36°9` in 54 cases, 37 to 37°9` in 22 cases. 8. Placenta previa was diagnosed by clinical symptoms and palpation of placenta in 58 cases, 7 cases by clinical symptom, placental palpation and placentography. 5 cases by clinical symptom and placentography, 3 cases during operation for abruptio placenta, premature rupture of membrane and C.P.D., and 3 cases in the trial of manual seperation of placenta after vaginal delivery. 9. Method of delivery included low transverse section in 27 cases (35.5%), classical seciton in 22 cases(29%), cesarean hystrectomy in 11 cases(14.5%) and vaginal delivery in 16 cases(21%). 10. Amniotomy was applied in an effort to control hemorrhage in 13 cases with successes, 10 cases within which 2 primigravidas were included, were delivered through vaginal rout, 3 by cesarean section. 11. The perinatal mortality rate was much lower in the fetuses over 2,500gm (1 1.4%) and 36 weeks of gestation(11.9%) than subgroup. The perinatal mortality rate was over 3 times higher in the total placenta previa as in the other varieties. 12. Expectant management for placenta previa was succesful only in 1 case. It was found wise to lengthem the gestational period, if possible, till 36 weeks with the patients hospitalized.

Association of low muscle mass with cardiovascular disease mortality in patients with diabetes

김한준,문신제 대한내과학회 2023 대한내과학회 추계학술대회 Vol.2023 No.2

에이즈 환자에서 발생한 비타민 B12 결핍에 의한 거대 적혈모구 빈혈

김봉영,김지은,황순우,이유화,박정환,문신제,최지영,송준석,정종헌,배현주 대한감염학회 2011 Infection and Chemotherapy Vol.43 No.3

Anemia is the most common hematologic finding in patients with advanced infections caused by human immunodeficiency virus (HIV) or advanced acquired immunodeficiency syndrome (AIDS). Among many etiologies of HIVassociated anemia, vitamin B12 deficiency plays an important role, mostly due to malabsorption or HIV enteropathy. We experienced a case of megaloblastic anemia caused by vitamin B12 deficiency in a male patient with an AIDS who had no structural gastrointestinal problem. He showed severe anemia, leukopenia, thrombocytopenia and suspicious neurologic manifestations such as aggravation of dementia and gait disturbance. With vitamin B12 and folate treatment, the patient’s general condition and hematologic features were improved successfully.