박육원통부재의 에너지흡수 개선에 관한 연구

기경서(K. S. Ki),김선규(S. K. Kim),양인영(I. Y. Yang) 한국정밀공학회 2000 한국정밀공학회 학술발표대회 논문집 Vol.2000 No.5월

Expression and mutational analysis of TGF-β/Smads signaling in human cervical cancers

기경도,동서연,허주엽,이선경,지성길,이종민 대한부인종양학회 2009 Journal of Gynecologic Oncology Vol.20 No.2

Objective: To define the molecular basis of TGF-β1 function in cervical carcinogenesis, we explored the expression and mutational status of TGF-β1, TGF-β1 receptors, and Smads, the regulators of the TGF-β1 signaling pathway, in human cervical cancers. Methods: Expression of TGF-β1, TGF-β1 receptors, and Smads transcripts were determined by quantitative reverse transcription-polymerase chain reaction (RT-PCR), and sequence alteration was analyzed using RT-PCR-single-strand conformation polymorphism (SSCP) analysis. Genomic levels of TGF-β1, TGF-β1 receptors and Smads was also measured by quantitative genomic PCR. Results: Abnormal overexpression of TGF-β1 and abnormal reduction of type II TGF-β1 receptor were identified in 36% (18 of 50) and 20% (10 of 50) of cervical cancer tissues, respectively. 22% (11 of 50) in Smad2 and 14% (7 of 50) in Smad4 revealed tumor specific mRNA reduction less than a half of normal means. In addition, no evidence for sequence alterations of the gene was found by RT-PCR-SSCP analysis. Conclusion: Our study demonstrates that disruption of TGF-β/Smad signaling pathway exist in human cervical cancer, suggesting that abnormal expressions of the member of TGF-β/Smad signaling pathway might contribute to the malignant progression of human cervical tumors via suppressing the tumor suppression function of TGF-β1 1's tumor suppression function. Objective: To define the molecular basis of TGF-β1 function in cervical carcinogenesis, we explored the expression and mutational status of TGF-β1, TGF-β1 receptors, and Smads, the regulators of the TGF-β1 signaling pathway, in human cervical cancers. Methods: Expression of TGF-β1, TGF-β1 receptors, and Smads transcripts were determined by quantitative reverse transcription-polymerase chain reaction (RT-PCR), and sequence alteration was analyzed using RT-PCR-single-strand conformation polymorphism (SSCP) analysis. Genomic levels of TGF-β1, TGF-β1 receptors and Smads was also measured by quantitative genomic PCR. Results: Abnormal overexpression of TGF-β1 and abnormal reduction of type II TGF-β1 receptor were identified in 36% (18 of 50) and 20% (10 of 50) of cervical cancer tissues, respectively. 22% (11 of 50) in Smad2 and 14% (7 of 50) in Smad4 revealed tumor specific mRNA reduction less than a half of normal means. In addition, no evidence for sequence alterations of the gene was found by RT-PCR-SSCP analysis. Conclusion: Our study demonstrates that disruption of TGF-β/Smad signaling pathway exist in human cervical cancer, suggesting that abnormal expressions of the member of TGF-β/Smad signaling pathway might contribute to the malignant progression of human cervical tumors via suppressing the tumor suppression function of TGF-β1 1's tumor suppression function.

Pulmonary Toxicity after a Quick Course of Combinatorial Vincristine, Bleomycin, and Cisplatin Neoadjuvant Chemotherapy in Cervical Cancer

기경도,이종민,이선경,동서연,허주엽,류정규,김교영 대한의학회 2010 Journal of Korean medical science Vol.25 No.2

Pulmonary toxicity is one of the most serious adverse effects associated with a quick course of vincristine, bleomycin, and cisplatin neoadjuvant chemotherapy (NACVBP). The aim of this study was to evaluate pulmonary toxicity related to a quick course NAC-VBP. A total of consecutive 61 patients, who underwent at most 3 cycles of NAC-VBP every 10 days in the International Federation of Gynecology and Obstetrics (FIGO) stage IB-IIB cervical cancer from 1995 to 2007, were retrospectively analyzed. Of the 61 study subjects, 7 (11.5%) were identified to have pulmonary toxicity and 2 (3.3%) died of pulmonary fibrosis progression despite aggressive treatment and the use of a multidisciplinary approach. No factor predisposing pulmonary toxicity was identified. Initial symptoms were non-specific, but bronchiolitis obliterans organizing pneumonia and interstitial pneumonitis were characteristic findings by high-resolution computed tomography of the chest. The benefit of steroid therapy was uncertain and was associated with steroid-induced diabetes mellitus requiring insulin therapy in two patients. Fatal pulmonary toxicity is a major concern of a quick course NAC-VBP. In conclusion, these patients require special monitoring for bleomycin-induced pulmonary toxicity.

GO-12 : Frequent Inactivation of the XAF1 Tumor Suppressor Gene in Human Ovarian Cancer by Aberrant Promoter CpG Sites Hypermethylation

기경도,이종민,동서연,이선경 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

XIAP-associated factor 1 (XAF1) is a tumor suppressor which has been known to exert proapoptotic effect. The XAF1 gene is located at 17p13.2 and its mRNA is ubiquitously expressed in all normal adult and fetal tissues, but absent or present at very low level in multiple human tumors. To explore the candidacy of XAF1 as a tumor suppressor in ovarian tumorigenesis, we investigated the expression and mutation status of the gene in 6 ovarian cancer cell lines and 16 tumor tissues. XAF1 transcript was easily detectable in all noncancerous ovarian tissues we examined. In contrast, however, its expression was not found or very low in 50% (3/6) of cancer cell lines and in 37.5% (6/16) of primary tumors. Whereas somatic mutations of the XAF1 gene were not detected, its mRNA expression was re-activated in no or low expression tumor cells following 5-aza-2-deoxycytidine treatment. Furthermore, bisulfite DNA sequencing analysis revealed that aberrant methylation at 7 CpG sites located in the 5` proximal region of the promoter is tightly associated with decreased mRNA expression, indicating that CpG sites hypermethylation of this promoter region is critical for the transcriptional silencing of XAF1. Together, our study suggests that epigenetic silencing of XAF1 by aberrant promoter methylation may contribute to the development and/or malignant progression of human ovarian tumors.

ROLE OF 5’-CpG ISLAND HYPERMETHYLATION OF THE FHIT GENE IN CERVICAL CARCINOMA

기경도,동서연,송동화,이종민,지성길,이선경 대한부인종양학회 2008 Journal of Gynecologic Oncology Vol.19 No.2

Objective: The abnormal expression of fragile histidine triad (FHIT) gene has been frequently reported in a variety of epithelial malignancies including cervical carcinoma. Furthermore, in a recent study it was proposed that transcriptional inactivation of FHIT, as a consequence of aberrant 5'-CpG island methylation, plays an important role in the carcinogenesis of human cervical carcinoma. The authors sought to determine whether abnormal FHIT transcription occurs in human cervical carcinoma, and if so, whether this abnormal expression is associated with aberrant 5'-CpG island methylation. In addition, the clinical significance of FHIT inactivation was investigated in Korean women with cervical cancer. Methods: To examine for abnormal transcripts of the FHIT gene, quantitative RT-PCR, genomic DNA-PCR and nonisotopic RT-PCR-SSCP analysis were performed using the standard method. The methylation status was determined by methylation specific PCR and bisulfite DNA sequencing. Results: The FHIT gene was down-regulated in 15 of 58 (25.9%) cervical carcinomas. FHIT promoter hypermethylation was detected in 15 of 15 (100%) abnormally expression in cervical carcinomas. Bisulfite DNA sequencing confirmed these findings and a significant correlation was found between CpG site hypermethylation and low FHIT expression. However, no significant correlation was found between reduced FHIT expression and clinicopathological characteristics. Conclusion: In this study, FHIT inactivation in cervical cancer was found to be strongly correlated with 5'-CpG island hypermethylation rather than a genetic alteration. Furthermore, no significant relation was found between a lack of FHIT expression and the prognostic factors of cervical cancer in our Korean cohort. Objective: The abnormal expression of fragile histidine triad (FHIT) gene has been frequently reported in a variety of epithelial malignancies including cervical carcinoma. Furthermore, in a recent study it was proposed that transcriptional inactivation of FHIT, as a consequence of aberrant 5'-CpG island methylation, plays an important role in the carcinogenesis of human cervical carcinoma. The authors sought to determine whether abnormal FHIT transcription occurs in human cervical carcinoma, and if so, whether this abnormal expression is associated with aberrant 5'-CpG island methylation. In addition, the clinical significance of FHIT inactivation was investigated in Korean women with cervical cancer. Methods: To examine for abnormal transcripts of the FHIT gene, quantitative RT-PCR, genomic DNA-PCR and nonisotopic RT-PCR-SSCP analysis were performed using the standard method. The methylation status was determined by methylation specific PCR and bisulfite DNA sequencing. Results: The FHIT gene was down-regulated in 15 of 58 (25.9%) cervical carcinomas. FHIT promoter hypermethylation was detected in 15 of 15 (100%) abnormally expression in cervical carcinomas. Bisulfite DNA sequencing confirmed these findings and a significant correlation was found between CpG site hypermethylation and low FHIT expression. However, no significant correlation was found between reduced FHIT expression and clinicopathological characteristics. Conclusion: In this study, FHIT inactivation in cervical cancer was found to be strongly correlated with 5'-CpG island hypermethylation rather than a genetic alteration. Furthermore, no significant relation was found between a lack of FHIT expression and the prognostic factors of cervical cancer in our Korean cohort.

회음부에 발생한 양성 섬유상피성 용종

기경도 ( Kyung Do Ki ),이선경 ( Seon Kyung Lee ),노동규 ( Dong Kyu Noh ),서상기 ( Sang Gi Seo ),양문호 ( Moon Ho Yang ),김윤화 ( Yoon Wha Kim ),유영천 ( Young Chun Yoo ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.2

A case of fibroepithelial polyp on vulvoperineal area is described. Her age is 20 year old and the tumor measured 9cm in its largest diameter and was connected to the left labium majus. This case is very unusual in that almost all reported vulvar fibroepi

편마비 환자에 대한 후방보행 훈련이 보행 속도와 균형 능력에 미치는 영향

기경일,김선엽,오덕원,김경환,Ki, Kyong-Il,Kim, Suhn-Yeop,Oh, Duk-Wyon,Kim, Kyung-Hwan 대한물리치료과학회 2009 대한물리치료과학회지 Vol.16 No.2

Background: The ability for backward walking is considered to be necessary for the neuromuscular control and maintenance of balance in daily ambulatory activity. This study aimed to determine the effect of backward walking training on the walking speed and balance control in patients with hemiplegia. Methods: Fourteen patients with hemiplegia were randomly allocated to an experimental and control groups of seven patients each. For the experimental group, we performed both conventional training and backward walking training, and conventional training only for the control group. The conventional training programs for the 2 groups were conducted for 30 min, twice a day, 5 times a week for 4 weeks, and backward walking training for the experimental group was conducted for 30 min, 3 times a week. The outcomes were assessed using the functional reach test (FRT), timed up-and-go (TUG) Test, and the 10 meter walk time test (10mWT). Result: A comparison of the FRT, TUG test, and 10mWT scores obtained before and after the 4-week treatment revealed statistically significant differences (p<.05) for the experimental group; however, there was no such difference in the case of the control group (p>.05). On assessment after the 4-week treatment, statistically significant differences were noted in the TUG test and 10mWT scores of the experimental group (p<.05). Conclusion: Our findings suggest that backward walking training is an effective clinical strategy for improving the walking speed and functional mobility of patients with hemiplegia.

제어기를 이용한 원통박육부재의 에너지흡수특성

기경서,김선규,양인영 조선대학교 에너지.자원신기술연구소 2000 에너지·자원신기술연구소 논문지 Vol.22 No.1

In this paper, collapse test of thin-walled circular tube member is performed under axially static in consideration of the collapse characteristics to develop the optimum structural members. Furthermore, the energy absorption characteristics is observed according to the controller, height of the controller, clearance between specimen and controller. thickness of the controller. As a result, it was clear that the energy absorption characteristics of circular tubes were influence by the controller, height, clearance, thickness in this study.

엉덩관절 각도에 따른 PNF 하지굴곡패턴운동이 체간 근활성도에 미치는 영향

기경일,조혁신,심선미,박현주,차현규,Ki, Kyong-Il,Cho, Hyuk-Shin,Sim, Sun-Mi,Park, Hyun-Ju,Cha, Hyon-Gyu 대한고유수용성신경근촉진법학회 2011 PNF and Movement Vol.9 No.3

Purpose : The purpose of this study was to analyze the effects of proprioceptive neuromuscular facilitation (PNF) leg flexion patterns according to the hip joint angle on electromyographic activity of the trunk. Methods : Thirty healthy adults volunteered to participate in this study. Subjects were required to complete following the PNF leg flexion patterns on three different hip joint flexion $30^{\circ}$, $60^{\circ}$ and $90^{\circ}$. An surface electromyogram (SEMG) was used to record the electromyographic activities of the trunk muscle in rectus abdominis, internal oblique abdominal, external oblique abdominal, erector spinae. The data were analyzed using the a repeated measures of one-way ANOVA with post-hoc Bonferroni's correction. Result : The results of this study are summarized as follows: The EMG activities of internal abdominal oblique and elector spinae muscle showed a statistically significant difference (p<.05). Conclusion : The result show that electromyographic activity of the trunk muscles significantly changed on PNF leg flexion patterns with difference hip joint angle. Therefore, this study used to basis for the intervention of the trunk muscle strength and stabilization.

복강내 전이된 난소의 악성 흑색종

기경도 ( Kyung Do Ki ),하창욱 ( Chang Wook Ha ),서상기 ( Sang Gi Seo ),허주엽 ( Chu Yeop Huh ),양문호 ( Moon Ho Yang ),이주희 ( Ju Hie Lee ),박지선 ( Ji Seon Park ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.7

Malignant melanoma is a neoplasm of the skin and mucous membrane which very rarely occurs in the ovary. Malignant melanomas occurring in the ovary must be differentiated from primary and metastatic malignant melanoma. Primary malignant melanoma of the ovary is extremely rare and is thought to originate from a cystic teratoma. Malignant melanoma of ovary without evidence of residual teratoma must be considered metastatic even in the absence of a previously cutaneous or mucocutaneous lesion. Opinions about its histogenesis, diagnostic criteria and elective treatment are controversial because of rare manifestations, and the prognosis remains poor in spite of the variety of therapeutic measures. After thoroughly surveying the specific literature, we report case of malignant melanoma with multiple metastases located in the stomach, the omentum and both ovaries with unknown site of primary origin.