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Sulindac에 의한 대장암 세포주 HT-29 세포의 세포사멸기작 연구 -미토콘드리아 통로를 중심으로-
박기재,권육,김성흔,김민찬,최홍조,김영훈,조세헌,정갑중,김성현,권혁찬 대한대장항문학회 2004 Annals of Coloproctolgy Vol.20 No.4
Purpose: This study was undertaken to reveal the molecular mechanism underlying sulindac-induced apoptosis in the human colon cancer cell line HT-29 (mutant p53). Methods: Apoptosis was determined by using Hoechst 33342 staining, and translocation of proteins was established by using immunofluorescence, immunoelectron microscopy, and Western blotting after ultra- fractionation. Results: This type of apoptosis was associated with decreased mitochondrial membrane potential, a translocation of the apoptosis-inducing factor (AIF) to the nucleus, and morphological evidence of nuclear condensation. However, DNA electrophoresis did not elucidate the ladder pattern of DNA fragments. Instead, a pulse-field gel electrophoresis showed that sulindac led to disintegration of nuclear DNA into-high- molecular-weight DNA fragments of about 100~300 kbp. Conclusions: Our findings indicate that sulindac induces large-scale DNA fragmentation, suggesting a predominantly AIF-mediated cell-death process, through translocation of the AIF to the nucleus in HT-29 cells.
Plummer-Vinson Syndrome과 동반된 진행성 위암 1예
이성근,이태무,권육,김기한,김민찬,정갑중,Lee Sung-Gun,Lee Tae-Mu,Kwon Yuk,Kim Ki-Han,Kim Min-Chan,Jung Ghap-Joong 대한위암학회 2004 대한위암학회지 Vol.4 No.2
Plummer-Vinson syndrome (sideropenic dysphagia) is characterized by dysphagia due to an upper esophageal or a hypopharyngeal web in a patient with chronic iron deficiency anemia. The main cause of dysphagia is the web of the cervical esophagus, and an abnormal motility of the pharynx or the esophagus is found to play a significant role in the above cause. Patients with this syndrome are thought to be precancerous because squamous cell carcinomas of the hypopharynx, oral cavity, or esophagus take place in $10\%$ of those patients. However, for Plummer-Vinson syndrome to be combined with gastric cancer is most unusual. We report the case of a 43-year-old woman who was first found to have stomach cancer under a diagnosis of Plummer-Vinson syndrome and who recovered after surgery.