Novel Simple Sequence Length Polymorphic (SSLP) Markers for Positional Cloning in Arabidopsis thaliana

권미,Hyun Kyung Lee,최성화 한국유전학회 2005 Genes & Genomics Vol.27 No.1

어유 및 과산화 어유를 섭취한 흰쥐에 있어서 플라보노이드 (+) - 카테킨의 산화안정 효과

권미나(Mee-Na Kwon),최재수(Jae-Sue Choi),변대석(Dae-Seok Byun) 한국식품영양과학회 1993 한국식품영양과학회지 Vol.22 No.4

4주령의 수컷 SD rat에 각각 옥수수유, 신선한 어유 그리고 과산화어유를 함유한 식이를 섭취시키면서 카테킨을 10㎎/㎏ 체중으로 복강내 투여하여 4주간 사육한 후 체중과 간장 무게의 변화, 혈청, 간장 및 부고환 지방조직에 대한 지질과산화물, 콜레스테롤, 중성지질, 인지질의 정량, 지방산 조성 및 GOT, GPT, SOD, catalase, glutathione peroxidase 등의 효소활성을 검토하였다. 체중증가는 옥수수유 섭취군이 어유 섭취군(FO)과 과산화어유 섭취군(PFO)에 비해 높은데 비해 간장무게는 그 반대의 결과였으며 카테킨의 투여에 의해 간장무게의 증가를 억제할 수 있었다. 지질과산화물 함량도 FO군에 비해 PFO군이 현저하게 높았는데 카테킨투여로 12~17%의 감소효과를 얻을 수 있었다. 카테킨 투여군(FO-C, PFO-C)은 FO, PFO군에 비해 총콜레스테롤 농도의 저하와 HDL-콜레스테롤 농도의 증가를 보임으로써 콜레스테롤 합성 억제 또는 대사의 촉진에도 효과적인 것을 알 수 있었다. 또한 카테킨 투여는 혈청의 중성지질과 인지질의 농도를 효율적으로 저하시킨 것으로 보아 지질 합성에 대한 강한 억제작용을 가진 것으로 나타났다. 또한 카테킨투여군이 비투여군에 비해 GOT활성이 낮아진 것으로 보아 카테킨투여가 간장의 손상을 억제할 수 있는 것으로 나타났다. 반면 생체내 과산화작용과 관련되어 유리기의 제거반응에 연관되는 생체의 SOD, catalase, GSH-Px등의 효소는 카테킨투여군이 오히려 낮은 활성을 보였는데 이 점을 homeostasis로 이해해야 할지 추후 계속 확인해 볼 필요가 있는 흥미로운 발견이었다. 혈청과 조직의 지방산 조성은 서로 조금씩 다른 것으로 나타났는데 혈청은 C_(18:1), C_(16:0), C_(18:2), C_(18:0)가 주요 지방산이었고 간장은 C_(18:0), C_(20:4), C_(16:0), C_(18:2)(인지질), C_(16:0), C_(18:2), C_(18:1), C_(22:6)(중성지질), 지방조직은 인지질과 중성지질 모두 C_(16:0), C_(18:1), C_(18:2)등이 주요 지방산으로 조직에 따라 약간씩 다른 경향을 보였다. 그리고 카테킨은 특히 혈청에서 C_(20:5), C_(22:6)과 같은 고도불포화지방산의 함량을 유지시키는데 효과적임을 알수 있었다. 이상의 결과를 미루어 볼 때 과산화 된 지질의 섭취는 생체내 대사 이상을 유발한 가능성이 인식되었고 카테킨의 투여는 신선한 어유를 섭취한 경우뿐만 아니고 이미 과산화된 어유를 섭취한 경우에도 생체내에서 과산화 억제작용을 어느 정도 효과적으로 수행하는 것으로 나타나 앞으로 이의 효과적인 활용이 기대된다. To evaluate the antioxidizing effect of flavonoid on fish oil and peroxidized fish oil, rats were fed with diets containing 5% corn oil (CO), 5% corn oil and 15% fresh fish oil (FO) or peroxidized fish oil (PFO) for 4weeks. An half of FO and PFO group rats were injected with 10㎎ flavonoid (+ )-catechin (a day per kg body weight) (FO-C and PFO-C). FO and FO-C group rats showed higher increase in body weight as compared to PFO, PFO-C group rats. Whereas, the opposite result was obtained in case of liver weight increase. In addition, catechin apparently reduced liver weight by 12~17%. Phospholipid, cholesterol, triglyceride and lipid peroxide content in serum and cholesterol, lipid peroxide content in liver and adipose tissue of PFO, PFO-C group rats were significantly higher than those of FO, FO-C one. These results suggested that catechin reduced the synthesis of lipid and protected effectively against lipid peroxidation. In fatty acids profile of neutral lipid and phospholipid, the ratio of polyunsaturated fatty acids (PUFA) versus saturated fatty acids (SFA) in PFO, PFO-C were lower than that of FO or FO-C because of ruduced PUFA. Contrary to our expectation, the enzyme activities of superoxide dismutase(SOD), catalase and glutathione peroxidase (GSH-Px) in rat liver of FO and FO-C group were lower than those of PFO and PFO-C group. These results were quite interesting and might be explained in terms of homeostasis. In case of total lipid in liver, C_(20:5), C_(22:6) fatty acids were decreased in rat fed peroxidized fish oil. In conclusion, catechin was considered to be an antioxidative and hepatoprotective drug and hypolipidemic agent.

수분-열처리와 노화에 의해 고아밀로오스 옥수수전분으로부터 형성된 효소저항전분의 특성비교

권미라,신말식,Kweon, Mee-Ra,Shin, Mal-Shick 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4

고아밀로오스 옥수수전분인 Hylon V와 Hylon VII을 사용하여 수분-열처리 및 노화하고 효소저항 전분을 분리하여 열적 특성 및 입자 형태를 각각 DSC와 현미경으로 조사하였다. 처리 후 효소저항 전분의 수율은 두 전분이 비슷한 경향으로 증가하였으나 아밀로오스 함량이 높은 Hylon VII이 Hylon V보다 더 높았다. 특히 수분-열처리에 의해 효소저항성이 크게 증가되어 생전분과 수분-열처리 전분의 효소저항 전분 수율은 Hylon V의 경우는 11.4%에서 26.6%로, Hylon VII의 경우는 15.9%에서 32.8%로 증가하였으나 노화전분은 생전분보다 약간 증가하였다. DSC에 의하면 수분-열처리로 호화온도가 증가되고 엔탈피가 감소하나, 노화된 전분은 $140^{\circ}C$ 근처에서 피크를 보였다. 생전분과 수분-열처리 전분에서 분리한 효소저항 전분은 폭넓은 곡선을 나타낸 반면, 노화전분에서 분리한 효소저항 전분은 $150^{\circ}C$ 근처에서 날카로운 피크를 나타냈다. 수분-열처리에 의해 전분 입자의 형태나 복굴절은 그대로 유지되었으나 노화전분은 호화 중에 용출된 아밀로오스에 의해 입자들이 서로 엉켜 있었다. 수분-열처리 전분에서 분리된 효소저항 전분은 요드 염색으로 남색을 보여 생전분이나 노화전분에서 분리된 효소저항 전분보다 효소저항성이 큼을 알 수 있었다. Thermal characteristics and granular morphology on enzyme-resistant starches (RS) formed during heat-moisture treatment (HMT) and retrogradation were investigated in high amylose corn starches, Hylon V and Hylon VII. With each treatment, both starches showed a similar trend in the increase of RS, but RS yield of Hylon VII is higher than that of Hylon V. Specially, RS was increased remarkably by HMT. It was more than doubled from 11.4% to 26.6% for Hylon V and from 15.9% to 32.8% for Hylon VII. A small increase of RS resulted from retrogradation. HMT on starch increased gelatinization temperature, decreased enthalpy. Retrograded starch exhibited small three endothermic transitions at $94^{\circ}C$, $110^{\circ}C$ and $140^{\circ}C$ in differential scanning calorimetry (DSC) thermogram due to the remained ungelatinized starch granules, dissociation of amylose-lipid complex and melting of recrystallized amylose, respectively. Enzyme-resistant starches isolated from native and heat-moisture treated starches showed a broad endothermic transition at higher temperature than native starch, while retrograded starch exhibited a very sharp peak at ${\sim}150^{\circ}C$ due to the melting of amylose crystallites. Under microscopy, starch granules with HMT was not changed, but retrograded starches showed the aggregates of starch granules because amylose leached out during gelatinization. Iodine stained RS clearly showed the differences in enzyme hydrolysis on the native, heat-moisture treated and retrograded starches.

A 10-Year-Old Girl’s Overcoming the Negative Mother Complex and Its Relationship to Ego Development

권미라 사단법인 한국임상모래놀이치료학회 2017 상징과 모래놀이치료 Vol.8 No.1

This case study is of the sandplay process of a young girl whose ego development was delayed predominantly, it seems, because of negative mother archetypal energy expressed by the personal mother. The client is an elementary school-aged girl caught in the negative mother archetype, particularly in its devouring quality which was a manifestation of the mother’s over-protectiveness; thus development of ego consciousness was impeded, as was a balanced relationship between ego and Self. The mother suffered from severe chronic depression and anxiety, and the father from panic disorder. As sandplay therapy progressed, client gradually began to engage in her everyday outer life and function normally, and the mother increasingly became less overprotective and more containing and accepting. As is evidenced in this case, sandplay therapy provided an environment which enabled the reconstitution of client's problematic primal relationship, constellation of the Self so that she developed a sense of self-worth and competency, and the reconstruction of her damaged ego-Self axis.

Succinylcholine 투여후 혈청내전해질 변화에 관한 연구

권미나,조정현,윤덕미,남용택 대한마취과학회 1988 Korean Journal of Anesthesiology Vol.21 No.6

여성적 생명력을 담은 치유의 상징: 버드나무

권미라(Kowen, Mee Ra) 한국임상모래놀이치료학회 2019 상징과 모래놀이치료 Vol.10 No.1

The symbolism of the willow, with its healing energy for addiction, can be considered in terms of its religious, folkloric, and mythological aspects as in Marion Woodman’s book The Ravaged Bridegroom -Masculinity in Women. This study concerned the healing process of a female client formerly obsessed with an addiction not separated from the Self. The client grasped the willow as a symbol of female energy and subsequently was reborn with renewed feminine vitality. The female healing energy of the willow connected her abandoned body to her soul. The vitality of the willow empowered the client to heal her animus of addiction, learn what her body wanted from the boundary of life and death, and realize what her soul desired. Her body, through consciousness raising, truly embraced the deity, or Self, which she had struggled unilaterally before to connect with herself. The totality, created by archetypes, meant a deep base of personality, accepted consciousness and unconsciousness and transcended the ego.

CAD에서 착화장치 성능 정밀화 확인 방안 연구

권미라(Mira Kwon),이승복(Seungbok Lee),류병태(Byung-tae Ryu) 한국항공우주학회 2018 한국항공우주학회 학술발표회 논문집 Vol.2018 No.11

이중언어 환경 아동의 모음 포먼트 특성에 관한 연구

권미지(Mi-Ji Kwon),고영옥(Young-Ok Ko),김혜경(Hye-Kyung Kim),이은정(Eun-Jeong Lee),정옥란(Ok-Ran Jeong) 한국음성학회 2007 음성과학 Vol.14 No.3

The aim of this study was to investigate the pitch and formant characteristics of vowels produced by monolingual and bilingual children. We collected sustained phonation of single vowels, /a/, /i/, /u/, from children aged 6 through 10 and compared their acoustic characteristics, fo, F1, F2. Results showed a significant difference between the groups in fo and F1 in the sustained phonation /a/, but not in F2. In the sustained phonation /i/, F2 revealed a significant difference but fo and F1 showed no significant difference. The F2 showed a significant difference in the sustained phonation /u/, but fo and F1 revealed no significant difference between the groups. It is needed to study further on the acoustic characteristics of bilingual children so that we can make a proper language intervention strategy for them.

뇌졸중 환자의 치료와 결과분석을 하는 물리치료사들의 EBP 활용도

권미지 ( Mi Ji Kwon ) 대한물리의학회 2012 대한물리의학회지 Vol.7 No.3

Purpose The purpose of this study was to identify education, attitudes and beliefs, interest and perceived role, self-efficacy and barriers to physical therapists` implementation of evidence-based practice for people with stroke. Methods The participants were 120 physical therapists providing services to people with stroke in Gwang-ju. The questionnaire contained items to evaluate practitioner and organizational characteristics and perceptions of research considered to influence evidence-based practice. The prevalence of practitioner and organizational factors was estimated using percentages. Logistic regression was used to examine relationships between sociodemographic and practice characteristics and each practitioner factor. Results 40% of respondents had learned the foundations of EBP in their academic preparation or received training in searching or appraising research literature. 88.3% agreed that research findings are useful. Self-efficacy ratings were 45%. Conclusion Lack of education, negative perceptions about research and physical therapists` role in evidencebased practice, and low self-efficacy to perform EBP activities represent barriers to implementating EBP for people with stroke that can be addressed through continuing education.

부모의 방임적 양육태도와 휴대전화의존 간의 관계에서 학습태도의 종단적 매개효과 검증

권미나,임선아 한국청소년상담복지개발원 2017 청소년상담연구 Vol.25 No.2

The purpose of this study was to verify the longitudinal mediating effect of learning attitude in the relationship between parent’s neglecting attitude and early adolescents’ mobile phone dependency. Specifically, this study aimed to identify whether the parent’s neglecting attitude influence the mobile phone dependency of the students, mediated by the learning attitude of the students longitudinally. To test this, from Korean Children & Youth Panel Survey (KCYPS), 2,013 students who responded to all three consecutive years; T2 (the 5th grade of elementary school), T3 (the 6th grade of elementary school), T4 (the 1st grade of middle school) were analyzed by utilizing Autoregressive Cross-Lagged Model (ARCLM). The results of this study were as follows. First, the previous parent’s neglecting attitude, learning attitude, and mobile phone dependency had significant effect on each parent’s neglecting attitude, learning attitude, and mobile phone dependency in the next grade stably and continuously. Second, the previous parent’s neglecting attitude had a significant negative effect on the subsequent learning attitude, and the previous learning attitude had a significant negative effect on the subsequent mobile phone dependency. Third, the parent’s neglecting attitude had significant effect on the mobile phone dependency of the children mediated by the learning attitude of the students longitudinally. Specifically, the learning attitude in the 6th grade of elementary school became more negative when the parent’s neglecting attitude in the 5th grade of elementary school was higher, and the mobile phone dependency in the 1st grade of middle school became higher when the learning attitude was more negative in the 6th grade of elementary school. This study has the implication of that it is important to change parent’s neglecting attitude to better conditions and to enhance students' learning attitude for preventing and managing the negative consequences of early adolescents' mobile phone dependency. 본 연구는 부모의 방임적 양육태도와 휴대전화의존 간의 관계에서 학습태도의 종단적 매개효과를 검증하고자 하였다. 구체적으로 초등학교 5학년 시기의 부모의 방임적 양육태도가 초등학교 6학년 시기의 학습태도를 매개로 중학교 1학년 시기의 휴대전화 의존도에 영향을 미치는지 확인하고자 하였다. 이를 위해 한국 아동·청소년패널조사(KCYPS)의 초등학교 4학년 패널 가운데 2차년도(초5), 3차년도(초6), 4차년도(중1) 모든 차수에 응답한 총 2,013명의 자료를 자기회귀교차지연모형(ARCLM)을 활용하여 분석하였다. 본 연구의 결과는 다음과 같다. 첫째, 이전 시점의 방임, 학습태도, 휴대전화 의존도는 이후 시점의 방임, 학습태도, 휴대전화 의존도 각각에 안정적이고 지속적으로 유의한 영향을 미치는 것으로 나타났다. 둘째, 이전 시점의 방임은 이후 시점인 학습태도에 부적으로 유의한 영향을 미치고, 이전 시점의 학습태도는 이후 시점인 휴대전화 의존도에 부적으로 유의한 영향을 미치는 것으로 나타났다. 셋째, 초 5 시점의 방임과 중 1 시점의 휴대전화 의존도 간의 관계에서 초 6 시점의 학습태도는 완전매개 역할을 하는 것으로 나타났다. 이는 초 5 시기에서 부모의 방임적 양육태도가 높을수록 초 6 시기의 학습태도는 부정적이 되고, 초 6 시기에서 학습태도가 부정적일수록 중 1 시기의 휴대전화 의존도는 높아지는 것을 의미한다. 본 연구는 자기회귀교차지연모형을 적용하여 부모의 방임적 양육태도와 휴대전화 의존도간의 관계에 있어 학습태도의 종단적 매개효과를 검증함으로써 실증적인 자료를 제공하였다는 점에서 그 의의를 가진다. 이를 통해 아동의 휴대전화 의존적 사용에 대한 부정적인 결과를 예방하고 대처하기 위해 부모의 방임적 양육태도 개선과 동시에 아동의 학습태도 증진이 중요함을 시사하고 있다.