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미니돼지에서 <sup>99m</sup>Tc-Tin colloid와 <sup>99m</sup>Tc-DISIDA를 사용한 간신티그라피의 비교 연구
심경미,김세은,이원국,궁성수,배춘식,이재영,최석화,한호재,강성수,박수현,Shim, Kyung-Mi,Kim, Se-Eun,Lee, Won-Guk,Koong, Sung-Soo,Bae, Chun-Sik,Lee, Jae-Yeong,Choi, Seok-Hwa,Han, Ho-Jae,Kang, Seong-Soo,Park, Soo-Hyun 한국생명과학회 2006 생명과학회지 Vol.16 No.6
동물 모델에서의 간기능의 비침습적 평가는 하나의 과제로 남아있다. 간신티그라피는 간의 크기 및 모양의 변화에 대한 정보를 제공할 뿐 아니라 간의 전체적인 기능을 파악할 수 있게 해 주며 간이식의 주요 합병증인 간염, 거부반응 및 담도계 합병증의 진단에도 편리하게 사용될 수 있다. 이에 본 연구에서는 미니돼지의 간 기능을 평가하기 위해 사람에서 주로 사용되는 방사성의약품인 $^{99m}Tc-Tin$ colloid와 $^{99m}Tc-DISIDA$를 이용하여 간 및 간담도 신티그라피를 시행하였다. 그 결과, 사람의 간신티그라피와 달리 미니돼지에서는 $^{99m}Tc-Tin$ colloid가 폐, 간, 위벽 및 신장에 섭취되고 폐와 간의 영상이 겹쳐져 간의 관심영역을 설정할 수 없었다. 그러나 $^{99m}Tc-DISIDA$를 사용한 간담도 신티그라피에서는 심장, 폐, 간, 담낭 및 십이지장으로의 연속적 방사능섭취를 관찰할 수 있었다. 따라서 미니돼지에서의 $^{99m}Tc-Tin$ colloid의 사용은 비특이적 결합력으로 인해 간 기능 평가를 위한 영상에는 적합하지 않으나 $^{99m}Tc-DISIDA$는 간담도 기능을 파악하는데 있어 좋은 수단이 될 수 있을 것으로 생각된다. Non-invasive evaluation of liver function in animal models remains a challenge. Hepatoscintigraphy provides information about changes in liver size and shape, and enables to understand general liver function. Futhermore it is readily used to diagnosis complications of liver transplantation like hepatitis, rejections and biliary complications. In this study, we investigated the usefulness of evaluating the liver function in miniature pigs with $^{99m}Tc-Tin$ colloid and $^{99m}Tc-DISIDA$ which are the most commonly used radiopharmaceuticals in human medicine. In result, $^{99m}Tc-Tin$ colloid was uptaked in lung, liver, gastric wall and kidney in miniature pigs. And $^{99m}Tc-DISIDA$ showed continuous uptake images of heart, lung, liver, gallbladder and duodenum, and it was similar to human's. Therefore we could conclude $^{99m}Tc-Tin$ colloid would not be suitable for evaluating hepatic function because of it's nonspecific affinity, however $^{99m}Tc-DISIDA$ scintigraphy would be an effective method for detecting hepatobiliary function in miniature pigs.
마우스 태생간 조혈세포이식시 이식거부 반응을 극복하기 위한 조혈세포 용량 및 흉선세포의 역할에 관한 연구
김승택(Seung Taik Kim),박선양(Seon Yang Park),김병국(Byong Kook Kim),이홍복(Hong Bock Lee),김은실(Eun Shik Kim),계경채(Kyung Chae Kye),서정선(Jeong Sun Seo),강위생(Wee Saing Kang),이현순(Hyun Soon Lee),궁성수(Sung Soo Koong),이재훈(J 대한내과학회 1990 대한내과학회지 Vol.39 No.5
N/A To observe whether an increase in cell doses can overcome graft rejection, whether sustained chimerism is present, and whether fetal thymic cell transplantation has any effect on the survival and the cell dose needed, fetal liver transplantation was done from 17-18-day old DDY fetus to an ICR mouse (syngeneic transplantationl) previously irradiated. The results revealed that better survival was obtained, regardless of syngeneic of allogeneic fetal liver cell transplantion, through an increase of the cell dose. A 90F long- term survial was noticed with a cell dose of 15×108/kg in syngeneic transplantation. In the case of allogeneic transplantation, however, only a 50% long - term survival rate was observed with the same dose. The concurrent thymic cell transplantation had a good effect on the long - term survival and the reduction of cell dose required, The cell dose needed for the achievement of a 90% long - term survival in syngeneic and allogeneic transplantation was 5x108/kg and 15x108/kg, respectively The sustained chimerism was evident and the graft-versus-host disease was mininal in fetal liver cell transplantation. It was evident that fetal liver cell transplantation could be one of substitution in solving the problems of bone marrow transplantation The cell dose required to prevent graft failure decreased with fetal thymic cell transplantation The mechanism of the salutary effect of concurrent thymic cell transplantation is not yet to be elucidated.
뇌의 Thyrotropin Releasing Hormone수용체 분포에 관한 연구
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),조보연(Bo Youn Cho),김승택(Seung Taik Kim),궁성수(Sung Soo Koong),유은숙(Eun Sook Yoo),박은미(Eun Mey Park),서일택(Il Tak Seo) 대한핵의학회 1990 핵의학 분자영상 Vol.24 No.1
N/A To evaluate the utility of autoradiographic technique in the detection of TRH receptor changes in brain after the various kinds of stimulation or drug administration, we tried the characterization of TRH receptor in mouse brain and autoradiography in rat brain as a preliminary study. The Kd value of [3-H] MeTRH to TRH receptors of adult male ICR mouse brain (cebellum and spina1 cord were excluded) was 3.55+0.6 nM and Bmax was 3.44+0.52 fmol/mg wet tissue by saturation analysis. The Kd value of TRH to TRH receptors was 133.8+28.2 nM by competition analysis. And we could visualize the distrbution of TRH receptors in rat brain by autoradiographic technique.
Technegas 환기스캔과 99mTc - DTPA Aerosol스캔의 비교
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),김상은(Sang Eun Kim),궁성수(Sung Soo Koong),최윤호(Yoon Ho Choi),김건열(Keun Youl Kim) 대한핵의학회 1990 핵의학 분자영상 Vol.24 No.2
N/A Pulmonary embolism demands rapid and accurate diagnosis. And ventilation imaging has greatly improved the diagnostic accuracy of pulmonary embolism in addition to perfusion imaging. Agents currently used include xenon-133, krypton-8lm and technetium-99m radioaerosols. However radioactive gases are compromised by availability and cost for krypton-8lm, radiation dose, gamma energy and non-physiologic behaviour for xenon-133. Radioaerosols of technetium-99m componds are rapidly cleared from the lung after inhalation, and their relative low effeciency (specific radioactivity) and wide distribution of particle sizes make them also suboptimum. A new ventilation agent, Technegas is a suspension of structured graphite ellipsoids with diameter below 20nm, labelled with Tc-99m in a carrier gas of Argon. This report describes the authors' clinical experience with Technegas. This is the first reported clinical study of this agent in Korea. A comparison of Technegas and Tc-99m-DTPA aerosol was performed in 12 patients with various pulmonary diseases such as COPD, pulmonary tuberculosis and pleural effusion. All patients were studied with Tc-99m-DTPA aerosol inhalation and Technegas ventilation. In both studies image quality was assessed (1) semiquantitatively by scoring bronchial and gastric activity, (2) subjectivly by direct visual comparisan of peripheral lung images and (3) quantitatively by computing the peripheral penetration index(PI) for each lungs. The bronchial activites were seen in 7 out of 12 cases with Tc-99m-DTPA aerosol and in 5/12 with Technegas. The gastric activities were seen in 5/12 and 1/12 cases respectively. The average values of PI were 61.26% with Tc- 99m-DTPA aerosol and 69.20% with Technegas (p〉0.05). Using Tc-99m-DTPA aerosol, COPD patients showed deposition in the central airways with poor visualization of the peripheral areas of the lungs. In Technegas studies these phenomena were less prominent, and the examination is well tolerated by patient and requires only a minimum of patient cooperation. With superiority of easy availability and handling, better physical characteristics and favorable image quality, Technegas is a promising agent for lung ventilation scanning.
김혜영,한진석,이정상,김현리,김진,이중건,이서진,김근호,진호준,전은실,주권욱,나기영,정우경,오지은,엄재호,궁성수 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.5
The purpose of this study was to elucidate whether the molecular defect of acid-base transporters in renal tubules is related to the functional defect of urinary acidification in distal renal tubular acidosis(RTA). We performed NH₄Cl, furosemide, or bicarbonate loading test to evaluate renal acidification function, and immunohistochemistry using antibodies to H^+ -ATPase, Cl^-/HCO₃^- exchanger(band-3 protein), and Na^+/K^+ -ATPase in kidney tissue in 6 patients with RTA and renal cell carcinoma patients as normal controls. Kidney tissue was obtained either by percutaneous needle biopsy(RTA) or nephrectomy(NC). The results were as follows; 1) In all six RTA patients, proton secretory defect of distal acidification was shown by a failure to lower the urine pH after NHC1 loading or furosemide test or abnormally low urine-blood pCO₂ difference during bicarbonate loading. In two patients with RTA, proximal acidification defect was combined, which was demonstrated by increased fractional excretion of bicarbonate. 2) In mal control, intense H^+ -ATPase and band-3 protein staining was observed in collecting ducts. 3) In distal RTA patients, H6+ -ATPase and band-3 protein staining was not demonstrable or markedly decreased in the intercalated cells of distal nephron. 4) In two patients who had both proximal and distal RTA, H^+ -ATPase staining was markedly decreased in the brush border of proximal tubules as well as the distal nephron. In conclusion, the defect of acid-base transporters in renal tubule was related with the functional defect of urinary acidification in distal RTA.