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Fluidigm 시스템을 이용한 고추 유전자원의 주요병 유전형 평가
금보라(Bora Geum),노나영(Na Young Ro),허온숙(On sook Hur),왕샤오한(Xiaohan Wang),최유미(Yu-Mi Choi),고호철(Hocheol Ko),이수경(Sukyeung Lee) 한국육종학회 2021 한국육종학회지 Vol.53 No.4
In this study, genotype analysis was conducted using the Fluidigm system with 13 SNP markers related to anthracnose, powderymildew, phytophthora root rot, cucumber mosaic virus (CMV), potyvirus, and tobacco mosaic virus (TMV) resistance. A total of 25,350 datapoints were obtained, 8.2% of which indicated resistance genotypes. Resources having perfect resistance genotype to each disease-related molecularmarker set were 58 accessions for anthracnose, 57 for powdery mildew, 246 for phytophthora root rot, and 765 for CMV. There was no resourcehaving a perfect resistance genotype of all three potyvirus-related molecular SNP markers including pvr1, pvr2 (123457), and pvr2 (689). Thirty-sevenaccessions for pvr1, 349 accessions for pvr2 (123457), and 23 accessions for pvr2 (689) were identified as resources with resistance genotype. No resource with the TMV-resistance genotype was found in this study. The resources with complex resistance genotypes of anthracnose and powderymildew were 55 accessions, and they were mainly Capsicum baccatum. Among these, seven accessions had a three-disease combined resistancegenotype with phytophthora root rot. Thirty-six accessions, 16 accessions, and nine accessions each had resistance genotypes of the three potyvirus-relatedmarkers. These resources also had CMV and phytophthora root rot resistance genotypes, and they were mainly Capsicum chinense. The statisticalanalysis considering the evaluated resistance genotypes, revealed that the anthracnose resistance genotype showed a tendency to be linked withpowdery mildew resistance genotype. The potyvirus-related marker pvr1 was positively correlated with Phytophthora root rot markers.
유색 호박 (Cucurbita spp.) 유전자원별 카로테노이드 대사체 분석
이민기 ( Minki Lee ),조수연 ( Sooyeon Cho ),조수정 ( Soojung Cho ),신재형 ( Jaehyeong Shin ),김헌웅 ( Heonwoong Kim ),김상규 ( Sanggyu Kim ),고호철 ( Hocheol Ko ),노나영 ( Nayoung Ro ),김정봉 ( Jungbong Kim ),백형진 ( Hyungjin Bae 한국환경농학회 2015 한국환경농학회지 Vol.34 No.3
BACKGROUND: Carotenoids which are a major source of vitamin A are contributed to have great potential role in anti-carcinogenic effects and eyesight. Carotenoids which can not synthesize in human body are required for food supply. The objectives of this study are to investigate compositions and contents of pumpkin (Cucurbita spp.) germplasms based on their pulp color. METHODS AND RESULTS: Carotenoids were extracted with 0.2% ascorbic acid in ethanol and saponified with 80% potassium hydroxide. Insoluble compounds were extracted into hexane. A total of nine carotenoids (three xanthophylls and six carotenes) were identified from pumpkin germplasms using HPLC equipped with photodiode array detector (450 nm). Especially, lutein and β-carotenes were major compound in germplasms. Among isomers of β-carotene, all-trans-β-carotene (16-27% of total carotenoids) was predominant compositions. Themean of total carotenoid contents was showed as brown (286.1 mg/100 g dw) > dark green (217.0) > orange (153.4) > primrose (85.8) > dark yellow (80.3). On the basis of carotenoid information, PLS-DA score plots showed different patterns by cluster in pumpkin germplasms. It was considered that these differences of phenotype were relative closely to genotype. CONCLUSION: This study indicated that dark color of pumpkin pulp was presented in high-level of biological pigments. It may contribute to develop potentially beneficial functional food ingredients.
동아시아 재래종 밀(Triticum aestivum L.) 유전자원의 고분자 글루테닌 조성과 숙기 특성 평가
이수경(Sukeyung Lee),최유미(Yu-Mi Choi),현도윤(Do yoon Hyun),이명철(Myung-chul Lee),오세종(Sejong Oh),고호철(Hocheol Ko),허온숙(On sook Hur),정연주(Yeonju Jung) 한국육종학회 2015 한국육종학회지 Vol.47 No.3
This study is to raise the utilization of genetic resources of wheat (Triticum aestivum L.) landrace from East Asia (Korea, Japan, and China) by evaluating genetic variation related to end use quality concerned to bread making quality and early maturity, two very important characteristics of Korean wheat cropping system. To clarify the allelic variation of Glu-1 loci which decides HMW glutenin subunit composition, SDS-PAGE and DNA marker analysis were conducted by using 485 East Asian landrace wheat accessions in National Agrobiodiversity Center, RDA and NIAS genebank. And useful accessions getting full mark of glu-1 score and early maturity were selected to enhance the utilization of genetic resources to Korean wheat breeding. In this study, wheats from China showed the distinct characteristic. Whereas Glu-A1c (null) and Glu-B1b (7+8) allele are the most frequent in Korean and Japanese accessions, Glu-A1a (2*) and Glu-B1c (7*+9) are the most in Chinese accessions. When it comes to unique composition, Glu-B1f (13+16) and Glu-D1e (2+10) subunits are only in Chinese resources. Glu-B1d (6+8), Glu-B1e (20), Glu-D1b (7+8), and Glu-D1c (4+1) subunits are only in Korean resources. The accessions from China also has high PIC value (0.53) compared to ones from Korea (0.35) and Japan (0.35). Grouping by UPGMA analysis of combination of Glu-1 allele, most accessions from Korea and Japan are in the same group, but most Chinese ones were distinguished as the distinctive group. The evaluation of bread baking quality by Glu-1 scoring system, 26 accessions got full marks. Among them, 16 accessions from China were also matured before early June, suitable to Korean cropping system. Especially, 3 accessions (K151847, K151865, K151962) had very early maturity, matured in late May. These genetic resources, having good gluten composition and early maturity, are expected to widely be used for Korean wheat breeding.
한국, 중국 및 엘살바도르 강낭콩 (Phaseolus vulgaris L.) 유전자원의 농업 특성과 다양성 비교
최유미(Yu-Mi Choi),이수경(Sugyeong Lee),현도윤(Do yoon Hyun),윤문섭(Munsup Yoon),오세종(Sejong Oh),이명철(Myung-Chul Lee),이정로(Jeongro Lee),고호철(Hocheol Ko),허온숙(Onsook Huh) 한국육종학회 2015 한국육종학회지 Vol.47 No.3
This study was conducted to compare the agricultural characteristics of total 444 common bean accessions from Korea(296), China (76), and El Salvador (72). Days to flowering were ranged from 41 to 83 days with an average of 61 days. Days to sowing to maturing were ranged from 86 to 143 days with an average of 104 days. Common beans from El Salvador tend to bloom and mature 3 to 7 days earlier than Korea and China accessions. In growth habit, over 50% of Korea and China accessions were indeterminate and climbing type, but 90.1% from El Salvador were Semi-determinate and climbing type. Qualitative traits were much different among three countries. Eighty-two percentage of immature pod colors were dark pink from El Salvador, but many of those were pale yellow from Korea (77.6%) and China (61.8%). Seed shapes were divided into four types of round, oval, cubic and kidney type, and the highest percentage of those were 30.4% with kidney type. The highest of those was 36.6% with oval type from Korea, 55.3% with kidney type from China and 79.2% with cubic type from El Salvador. Morphological characteristics of common bean from El Salvador were much different from those of Korea and China, which is necessary to collect more germplasm from its native and expand genetic diversity of common beans. Four hundred thirty-five common beans from Korea, China and El Salvador were analyzed using SSR markers. Ninety-two alleles were detected with a lowest of 6 at the BM161, BM181 and a highest of 18 at the BM154, BM160. The average polymorphism information content(PIC) was 0.72. To similar with population size among three countries, 292 Korean accessions divided four replications with 73 accessions. As a result genetic diversity was the highest of 0.73 in Chinese populations, while the lowest of 0.48 in El Salvador populations.