家鬼 各組織의 C^(14) 標識 酸代謝過程에 關한 實驗

高澤容 大韓産婦人科學會 1965 大韓産婦人科學會雜誌 Vol.8 No.8

The Clinical Implications of Death Domain-Associated Protein (DAXX) Expression

고택용,김종인,박억숭,문정민,박성달 대한흉부외과학회 2018 Journal of Chest Surgery (J Chest Surg) Vol.51 No.3

Background: Death domain-associated protein (DAXX), originally identified as a pro-apoptotic protein, is now understood to be either a pro-apoptotic or an anti-apoptotic factor with a chromatin remodeler, depending on the cell type and context. This study evaluated DAXX expression and its clinical implications in squamous cell carcinoma of the esophagus. Methods: Paraffin-embedded tissues from 60 cases of esophageal squamous carcinoma were analyzed immunohistochemically. An immune reaction with more than 10% of tumor cells was interpreted as positive. Positive reactions were sorted into 2 groups: reactions in 11%–50% of tumor cells and reactions in more than 51% of tumor cells, and the correlations between expression and survival and clinical prognosticators were analyzed. Results: Forty-three of the 60 cases (71.7%) showed strong nuclear DAXX expression, among which 19 cases showed a positive reaction (31.7%) in 11%–50% of tumor cells, and 24 cases (40.0%) showed a positive reaction in more than 51% of tumor cells. A negative reaction was found in 17 cases (28.3%). These patterns of immunostaining were significantly associated with the N stage (p=0.005) and American Joint Committee on Cancer stage (p=0.001), but overall survival showed no significant difference. There were no correlations of DAXX expression with age, gender, or T stage. However, in stage IIB (p=0.046) and stage IV (p=0.014) disease, DAXX expression was significantly correlated with survival. Conclusion: This investigation found upregulation of DAXX in esophageal cancer, with a 71.7% expression rate. DAXX immunostaining could be used in clinical practice to predict aggressive tumors with lymph node metastasis in advanced-stage disease, especially in stages IIB and IV.

性의 早熱

高澤容,吳世娟 中央醫學社 1965 中央醫學 Vol.8 No.5

Relationship between Cancer Stem Cell Marker CD133 and Cancer Germline Antigen Genes in NCI-H292 Lung Cancer Cells

고택용,김종인,이상호 대한흉부외과학회 2020 Journal of Chest Surgery (J Chest Surg) Vol.53 No.1

Background: Previous studies have shown that lung cancer stem cells express CD133 and that certain cancer stem cells express cancer germline antigens (CGAs). The transcriptional regulation of CD133 is complicated and poorly understood. We investigated CD133 and CGA expression in a non-small cell lung cancer cell line. Methods: The expression levels of CD133 and CGAs (MAGE-6, GAGE, SSX, and TRAG-3) were measured in an NCI-H292 lung cancer cell line. The methylation status of the CD133 gene promoter region was analyzed. The expression levels and promoter methylation statuses of CD133 and CGAs were confirmed by treatment with the demethylating agent 5-aza-2'-deoxycytidine (ADC). Results: After treatment with ADC, CD133 expression was no longer detected. MAGE-6 and TRAG-3 were detected before ADC treatment, while GAGE and SSX were not detected. ADC treatment upregulated MAGE-6 and TRAG-3 expression, while GAGE expression was still undetected after treatment, and only weak SSX expression was observed. GAGE expression was not correlated with expression of CD133, while the levels of expression of MAGE-6, TRAG-3, and SSX were inversely correlated with CD133 expression. Conclusion: These results showed that CD133 expression can be regulated by methylation. Thus, the demethylation of the CD133 promoter may compromise the treatment of lung cancer by inactivating cancer stem cells and/or activating CGAs.

정상자궁내막 선상피핵용적에 대하여

고택용(TY Koh) 대한산부인과학회 1962 Obstetrics & Gynecology Science Vol.5 No.4

세포핵의 용적은 그 세포의 기능을 정확하게 파악할 수 있는 표준이 된다. 자궁내막의 주기적변동에 따라 핵용적에도 주기적 변동이 있으리라고 추축되어 연자는 자궁내막선상피 핵용적의 주기적변동을 관찰하였다. 조직학적으로 정형적인 소견을 갖인 조기증식기내막, 만기증식기내막, 조기분비기내막 및 만기분비기내막 각 5예를 선정하여 Haematoxylin-Eosin염색을 하고 Zeiss-Winkel의 계측기를 사용하여 핵용적을 계측하였다. 그의 성적은 다음과 같다. 즉 조기증식기내막 62,80 만기증식기내막 88,32 조기분비기내막 88,32 만기분비기내막 44,16 이였다. 이상으로 보아 자궁내막 선상피세포의 핵용적은 조기증식기에서 부터 점차 증가되었다가 만기분비기에 이르러 점차 감소됨을 알 수 있다.

가토 각조직의 C14표지유산대사과정에 관한 실험

고택용(TY Koh) 대한산부인과학회 1965 Obstetrics & Gynecology Science Vol.8 No.8

Twenty nine of rabbits were divided into 3 groups; the first. group for liver tissue incubation experiment, the second group for kidney incubation experiment and the third group for brain incubation experiment. The same tissue slices obtained from each run of every group were subdivided into 3 subgroups. Each subgroup was incubated for a period of 2 hours in the constant temperature water bath with incubation mixtures consisting of 10 cc of phosphate buffer and 20 cc of oxygen, to which were added Lactate-1-C14 in the subgroup "a", Lactate-2-C14 in the subgroup "b", Lactate-3-C14 in the subgroup "c" respectively. The gas samples and incubation medium obtained at the end of incubation period from each subgroup were analysed for total CO2 production rates, redioactivities of CO2 produce and lactate disappearance rates. Data obtained from each groups were summerized as follows. 1) The mean value of total CO2 production rates were, 27.8±9.3 μM/hr/gm in the first group, 43.6±7.8 in the second group and 45.3-6.7 in the third group. The fractions of CO2 derived from C14-lactate to total CO2 production were 23.9 % in the first group, 52.9% in the second group and 63.9% in the third group. These data explained that carbohydrate such as lactate contribute a minor part for the release of their oxidative energy in the liver tissues, however more than 50% of oxidative energy were released by lactate in the kidney and brain tissues. 2) In each subgroups of vrious tissues, comparing the C14O2 production rates from each carbon of lactate, C14O2 yielded from the subgroup "a" were generally larger than that from the subgroups "b" and "c". This effect explaines that carboxyl carbon of lactate contribute larger part to produce the respiratory CO2 from lactate in the various tissues. While, comparing the C14O2 yielded from non carboxyl carbons of lactate in the subgroups "b" and "c" in each group, the C14O2 derived yielded from βcarbon of lactate was larger than that from the α carbon of lactate in the liver tissue. There were little difference in C14O2 yield from the α and β carbon in the kidney tissue, but C14O2 yielded from α carbon of lactate was larger than that from the βcarbon of lactate in the brain tissue. Possible explanations for these effects were briefly discussed. 3) The fractions of disappeared lactate from incubation medium into respiratory CO2 which were expressed by relative lactate disapperance (RLD) were 18.1% in the first group, 43.5% in the expressed by relative lactate disapperance (RLD) were 18.1% in the first group, 43.5% in the expressed byrelative lactate disapperance (RLD)were 18.1% in the first group, 43.5% in the second group and 43.2% in the third group. Thus less than 20% of disappeared lactate was oxidized into respiratory CO2 and remainder of lactate disappeared was incorporated into synthetic proess in the liver tissue. More than 40% of lactate disappeared was, however, utilized to release of their oxidative enegy in the kidney and brain tissues.

복강임신의 1예

고택용(TY Koh),김은배(EB Kim),김숭경(SK Kim) 대한산부인과학회 1964 Obstetrics & Gynecology Science Vol.7 No.10

복강임신은 매우 희유한 것이며 Beacham 씨는 전 분만 41,000예중 20예를 보았다고 하며 William B. Stromn 씨는 분만예 3,000~15,000 분만에 1예라고 보고 하였다. Cambell 1:36,480, Estman 1:1,500, John 1:1,746 난관임신에 대한 복강임신의 빈도는 Johcho 0.01%. Amstrong 0.02%, 우옥 3%, 동대 5.4%의 발생빈도를 보고 하였다. Douglass and Mohn 1920년~1944년의 25년간 Baltimore Hospital 등 여러 Hospital 에서 모인 증예를 토대로 나타난 통계에 의하면 인구 100만이 있는 1개 도시에서 1년에 1예의 발생율을 보였으며 흑인은 4,188명의 신산아중 1명이 복강임신이었고 백인은 67,534명 중 1명으로 흑인이 백인보다 16배의 발생율이 있음을 보고하였다. 복강임신은 원발성인 것과 속발성인 것으로 구분할 수 있으며 원발성 복강임신은 최초부터 복막면상에 착상 발육하는 것으로 그 빈도는 극히 희유하고 대부분은 속발성인 것으로 자궁 난소 난관의 착상부위로부터 유산, 압출, 파열에 의하여 이탈하여 복막에 달하고 이차적으로 발육하는 것이다. 보통은 난관 유산후 태아는 사망하거나 후에 서서히 흡수된다. 간혹 일정기간 생존한다고 해도 mummification되거나 lithopedion 등의 경과를 취하는 것이 보통이다. 복강내에서 태아의 발육이 지속되어 임신말기에 이르는 것은 희유하다. Becham and Beacham은 130예에 대해 태아 사망율은 85%라 했고 Eastman은 1935년 이래 249예를 analysis 해 보니까 75.6%라 했고 term까지 가더라도 fetal abnormality가 50% 이상을 차지한다고 하였다. 모체의 사망율은 Albert는 약 10%, Warre는 14.85% 정도라고 보고하였다. 본예는 복강내에서 태아가 임신 39주까지 지속이 되어 어떤 기형도 없이 만출되어 모자 공히 건강히 유지한 희유하고 흥미 있는 복강내 임신의 1예임으로 이에 보고한다. ...자궁외임신... A case of advanced secondary abdominal pregnancy at term is an unusual dramatic problem. Because of the increasing frequency of this condition, it seems important to review the literature and report a case with a living child...., ectopic pregnancy....

Zyklische A¨nderungen der Kerngrosse in Dru¨senepithel des menschlichen Uterus

Koh, Taikyong 大韓産婦人科學會 1962 大韓産婦人科學會雜誌 Vol.5 No.4

Unusual Communication between the Pulmonary Artery and Vieussens’ Arterial Ring Causing Infective Endocarditis

이상호,고택용,조성호 대한흉부외과학회 2019 Journal of Chest Surgery (J Chest Surg) Vol.52 No.6

Coronary artery fistula is an abnormal communication between the coronary artery and the cardiac chambers. In particular, an abnormal connection between the conus branch of the right coronary artery and the proximal left anterior descending coronary artery is defined as Vieussens’ arterial ring. Coronary artery fistulas are usually asymptomatic, but some can cause complications such as infective endocarditis. Here, we report a case of Vieussens’ arterial ring causing infective endocarditis with severe mitral regurgitation.

Saccular Aneurysm of the External Jugular Vein: A Case Report

이해영,고택용,김현수,김종인,박성달,조성래,천봉권,조성호 대한흉부외과학회 2014 Journal of Chest Surgery (J Chest Surg) Vol.47 No.2

Saccular aneurysm of the external jugular vein presenting as a neck mass is very rare. We report the surgical treatment of an external jugular venous aneurysm in a 48-year-old female patient due to the cosmetic problem of neck engorgement, concomitant with thyroidectomy for cancer.