Clinical Utility of Chimerism Status Assessed by Lineage-Specific Short Tandem Repeat Analysis: Experience from Four Cases of Allogeneic Stem Cell Transplantation

고리영,조성숙,송유정,허경,권혁찬,김효진,오성용,김성현,한진영 대한진단검사의학회 2009 Annals of Laboratory Medicine Vol.29 No.4

Chimerism testing permits early prediction and documentation of successful engraftment, and also facilitates detection of impending graft rejection. In this study, we serially monitored chimerism status by short tandem repeat-based PCR in nucleated cells (NC), T cells and natural killer (NK) cells after myeloablative allogeneic stem cell transplantation (SCT). Four patients with myeloid malignancies showed discrepant chimerism results among those three fractions. Three patients had mixed chimerism (MC) of donor/host T cells at a time point around the onset of chronic graft-versus-host disease (GVHD). In two patients with disease relapse, MC of NK cells preceded a morphological relapse or NK cells showed a higher percentage of patient cells compared to NC. Therefore, our study shows that chimerism analysis in lineage-specific cells might be useful in predicting clinical outcome after allogeneic SCT in certain patients. Chimerism testing permits early prediction and documentation of successful engraftment, and also facilitates detection of impending graft rejection. In this study, we serially monitored chimerism status by short tandem repeat-based PCR in nucleated cells (NC), T cells and natural killer (NK) cells after myeloablative allogeneic stem cell transplantation (SCT). Four patients with myeloid malignancies showed discrepant chimerism results among those three fractions. Three patients had mixed chimerism (MC) of donor/host T cells at a time point around the onset of chronic graft-versus-host disease (GVHD). In two patients with disease relapse, MC of NK cells preceded a morphological relapse or NK cells showed a higher percentage of patient cells compared to NC. Therefore, our study shows that chimerism analysis in lineage-specific cells might be useful in predicting clinical outcome after allogeneic SCT in certain patients.

COVID-19 판데믹이 기증제대혈은행에 미치는 영향

고리영,김정아,박수지,심재룡,곽민선,김경희 대한수혈학회 2022 大韓輸血學會誌 Vol.33 No.2

The COVID-19 pandemic has had a major impact worldwide, making it difficult to transplant unrelated hematopoietic stem cells. On the other hand, cord blood transplantations in Korea increased during the pandemic. In 2021, the release of 110 cord blood units and 83 cord blood transplantations were performed. Cord blood transplants have increased by 35% compared to the pre-pandemic period (average of 58 cases over four years vs. 78 cases over two years). This phenomenon is not only occurring in Korea. In France, cord blood transplantation in 2020 increased by 19% compared to the previous year. The cord blood is the blood in the umbilical cord and placenta that would be discarded during childbirth but can be used as a useful source of hematopoietic stem cells in the COVID-19 pandemic. In addition, it is essential to collect and store high-quality cord blood continuously because of the high likelihood of developing various therapeutic agents using cord blood. (Korean J Blood Transfus 2022;33:114-117)

비골수억제 동종조혈모세포 이식 후 Mutiplex STR PCR을 이용한 T세포 키메리즘 분석

고리영,한진영,한훈,김재석,권혁찬,김성현,김효진 대한혈액학회 2006 Blood Research Vol.41 No.1

배경: 비골수억제 조혈모세포 이식을 시행한 환자의 종양 세포를 사멸시키는 기전은 GVM 효과에서 기인한다. MC가 지속되면 GVHD의 위험성은 감소되지만 종양에 대한 내성이 초래되어 결과적으로는 GVM 효과가 감소될 우려가 있다. 따라서 이식 후 키메리즘 분석은 면역 억제 중단 시기와 DLI 시기 등을 결정하는 데에 필수적이다. 방법: 비골수억제 조혈모세포 이식을 시행한 총 17명의 각종 종양 환자를 대상으로 이식 후 정기적으로 말초혈액의 T세포 키메리즘 상태를 분석하였다. T세포로부터 DNA를 증폭하였고, 증폭 산물은 자동 형광 분석 장비로 분석하였다. 결과: T세포 MC는 이식 후 모든 환자에서 관찰되었으며, MC 정도와 지속 기간 등에 따라 세 군으로 나뉠 수 있었다. 1군은 5명의 환자였고, 잠깐 동안 T세포 MC를 보인 후 중앙값 25일을 전후하여 CC에 도달하였다. 2군은 5명이었고, T세포 MC가 짧은 동안 보이다가 중앙값 21일을 전후하여 환자 세포로 다시 대치되면서 CC에 전혀 도달하지 못하고 본래 질환이 재발하거나 다시 진행하였다. 3군은 7명으로서, 이식 후 40~50일경에 지속적으로 MC를 보이다가 이후 중앙값 112일 전후하여 CC에 도달하였다.

Biodiesel production with ultrasound induced process

최승훈,고리영,이재문,이승범,최준호,홍인권 한국공업화학회 2008 한국공업화학회 연구논문 초록집 Vol.2008 No.1

Determination of the Prevalence of Aspirin and Clopidogrel Resistances in Patients with Coronary Artery Disease by using Various Platelet-function Tests

우광숙,김보람,김지은,고리영,Long-Hao Yu,김무현,한진영 대한진단검사의학회 2010 Annals of Laboratory Medicine Vol.30 No.5

Background: Dual therapy with aspirin and clopidogrel has emerged as the gold standard therapy for patients treated with drug-eluting stents (DES). However, there is variability in patients’ responses to this antiplatelet therapy, and some patients continue to show ischemic recurrences after therapy. The purpose of the study was to compare the simultaneously obtained results of various plateletfunction tests for assessing the prevalence of antiplatelet resistance in coronary artery disease patients undergoing DES therapy. Methods: A total of 66 patients were administered a loading dose of aspirin, clopidogrel, and cilostazol at least 12 hr before stenting. The results of VerifyNow (Accumetrics, USA), multiplate analyzer (Dynabyte Medical, Germany), and vasodilator-stimulated phosphoprotein/P2Y12 (Biocytex,France) assays were compared with those of light transmission aggregometry (LTA) analysis. Results: The P2Y12 reaction units and P2Y12% inhibition values obtained using the VerifyNow assay showed strong correlation (r) with the results of the LTA analysis. All tests results showed low concordance in defining the antiplatelet resistance in patients, and the degrees of agreement were as follows: 0 for aspirin reaction units; 0.25, P2Y12% inhibition; 0, aspirin-sensitive patients’ identification test; 0.21, ADPtest; and 0.14, platelet reactivity index, expressed as the k statistics. The prevalence of aspirin and clopidogrel resistances in patients resulted in remarkable variations, from 0%to 22.7% and from 9.1% to 48.5%, respectively. Conclusions: The clinical usefulness of the different assays for the correct classification of patients in terms of antiplatelet resistance remains unclear. Further studies are required to determine the best method for correlating the occurrences of adverse ischemic events. (Korean J Lab Med 2010;30:460-8)

한국 공공 제대혈은행의 보관 정책 개선: 장기 보관 및 단기 보관 제대혈의 품질 비교

이미남,김경희,김병권,고리영,김준년 대한수혈학회 2020 大韓輸血學會誌 Vol.31 No.2

Background: The decreased use of cord blood units (CBU) due to improvements in haploidentical transplantation is a financial burden for public cord blood banks. Currently, there is no guidance regarding the length of cryopreservation of CBU in Korean public banks. The relative quality of long-term storage CB (LTCB) and short-term storage CB (STCB) needs to be evaluated to establish a storage policy. Methods: Thirty-four and thirty-one units of CB cryopreserved for less than one year and up to 14∼15.5 years, respectively, in the Busan Gyeongnam Public Cord Blood Bank were assessed. The total nucleated cells (TNCs), CD34+ cell counts, and colony-forming units-granulocyte monocyte (CFU-GM) were examined. The cell viabilities were evaluated by Eosin-Y exclusion staining and 7-aminoactinomycin D flow cytometry. The number of stored Korean public CB units from 2000 to 2016 was determined and categorized according to TNCs. Results: The post-thawing viability of the STCBs measured by flow cytometry was consistently higher than that of the LTCBs (TNCs, 62.5% vs 57.3%; MNCs, 93.1% vs 88.9%; CD34+ cells 95.7% vs 94.0%). The CD34+ cell viability was significantly higher in STCB (P=0.03). The CFU-GM after thawing was higher in STCBs (61.5±23.4 vs 49.9±22.8 [0.95 mm2 ] P=0.05). Of the 48,161 CB units stored until 2016, Dec, 9,493 (19.7%), which were stored until 2006, had been stored for more than 10 years. Conclusion: LTCB with a low number of cells (<0.7×109 cells) should be considered to exclude from storage for therapeutic purposes to improve the storage efficiency. (Korean J Blood Transfus 2020;31:119-130)

Aspirin Resistance May Not Be Associated with Clinical Outcome after Acute Ischemic Stroke: Comparison with Three Different Platelet Function Assays

유남태,배효진,김지은,고리영,한진영,김무현,차재관 대한뇌졸중학회 2012 Journal of stroke Vol.14 No.1

Background: Aspirin resistance (AR) in platelet function assays showed substantial variation depending on the methods used to evaluate it. Methods: In this study, we prospectively compared the results of Multiplate impedance platelet aggregometry (IPA) with those of light transmission aggregometry (LTA) and VerifyNow® system in determination of the prevalence of aspirin resistance (AR) and investigated the correlation between its presence and poor outcome (modifi ed Rankin scale >2) in 105patients with aspirin after acute ischemic stroke (AIS). Results: After 5 days of using aspirin, 15 patients (14.3%) were classifi ed as aspirin-resistance with the use of IPA, 24 patients (22.9%) by the LTA, and 14 patients (13.3%) by VerifyNow. Good agreement between the results of IPA and VerifyNow, was found (R=0.674, P <0.01). The concordance rate of AR detection was high between VerifyNow and IPA (k=0.72, P <0.01), albeit quite low between LTA and IPA. Regarding on its infl uence on clinical outcome after AIS, there wasn’t any signifi cant relationship between occurrence of poor outcome and the presence of AR in three platelet function assays. Conclusion: This study reveals that the incidence of AR in AIS might be highly test-specifi c. IPA seems to be similar to VerifyNow as a platelet function test.