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한우의 수정란 이식에 관한 연구 제3보 . 수정란의 비외과적 채취와 이식
고광두,정길생,이기만 ( Gwang Du Goh,Kil Saeng Chung,Ki Man Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4
For the purpose of obtaining the artificial twin birth in the transplanted Korean native cows, non-surgical obtaining of the fertilized eggs and their transplantations were performed. The results were as follows: 1. Average six eggs per donor were ovulated and 65% of them were recovered by non-surgical technique. 2. Eighty two percent of eggs recovered were found to be morphologically normal and were at 4-32 cell stage, respectively. 3. Seventy five percent of cattle treated with prostaglandin F₂α were synchronized in their estrus within 4 days after administration. 4. No twin fetation was obtained from the recipients which received fertilized eggs.
한우의 수정란 이식에 관한 연구 제1보 . GTH 단독투여에 의한 한우의 쌍태 유기
고광두,정길생,이기만 ( Gwang Du Goh,Kil Saeng Chung,Ki Man Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4
For the studies of twin birth induction on Korean native cattle, the following processes were taken: first, PMS were injected subcutaneously to promote the growth of follicles, then HCG were injected into the vein for the superovulation and the artificial insemination were performed for conception. The results were gained as follows: 1. Average duration from PMS administration to onset of estrus was 3.8 to 4.7 days and no differences due to dosage of PMS were observed. 2. Duration of induced estrus was ranged from 3.3 to 4.6 days and the length of induced estrus cycle was shortened than that of normal estrus. 3. The most grown follicles (5.3 ∼ 5.5) were observed in the cattle administrated with 2,000 IU of PMS and 1,000 IU of PMS was considered to be the optimum dosage for the limited ovulation. 4. Ovulation rate was declined when the PMS dosage was increased more than 1,000 IU. 5. The highest rate of twin birth (35.7%) was obtained from the cattle administrated with 1,000 IU of PMS and the next was the cattle administrated with 2,000 IU and 750 IU of PMS, respectively.
한우의 수정란 이식에 관한 연구 제1보 . GTH 와 PGF 2α 투여에 따른 난소반응
고광두,정길생,이기만 ( Gwang Du Goh,Kil Saeng Chung,Ki Man Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4
For the induction of follicle development and heat, PGF₂α was injected intramuscularly two days after PMS was injected by subcutaneous or intra-ovary methods on multiparous cows during of 9-13 days of the estrus cycle. After the above treatments, intravenous HCG injection for the superovulation was followed. The responses of ovaries were as follows: 1. Average duration from PMS administration to the onset of estrus was 5 days and the duration was shortened with the increasing dosage of PMS. 2. Duration of induced estrus was ranged from 2.3 to 4.8 days and the duration of estrus was shortened with the decreasing dosage of PMS. 3. Number of follicle obtained from the cattle treated with 4,000, 3,000, 2,000, 1,000 and 500 IU of PMS were clarified to be 11.4, 11.5, 7, 2.3 and 2.5, respectively, after 4.8 - 5.2 days of PMS administration. 4. Observing the results of slaughter, ovulation rates of the cattle treated with 4,000, 3,000, 2,000, 1,000, and 500 IU of PMS were 16.3, 35.6, 60.0, 65 and 75%, respectively. 5. The numbers of degenerated, or bled follicle were increased with the increase of PMS dosage.
대관령지구(大關嶺地區) 고령지(高嶺地) 한우(韓牛) 번식장해(繁殖障害)에 관(關)한 연구(硏究) - 대단위목장(大單位牧場)을 중심(中心)으로 -
고광두,Goh, Gwang Du 대한수의학회 1975 大韓獸醫學會誌 Vol.15 No.2
Infertility and breeding status of Korean cows which are reared in a extensive alpine ranches of Daegwanryeong area was investigated. The results obtained were summarized as follows, 1. Infertility frequency of the cows was 25.9% against a total examined number of 675 heads, and was 17.6% against a total rearing number. 2. The distributions of infertile cows 44.6% of Granular vaginitis-like syndrome, 36% of repeat breeder, 8.6% of symptom of ovary, 6.8% of symptom of uterus and 4% of other symptoms. 3. According to its age were found by 51.4% upon 2 years of age, 30.3% upon 3 years of age, 11.4% upon 4 years of age and 6.9% upon S years of age. 4. Breeding status of Korean cows showed 74.1% of conception rate in the cases of pre-treatment, while breeding status increased markedely in the cases of post-treatment by the 85.8%. 5. The conception rate by the artificial insemination mainly on the first mating was 36.1%. on the second mating 23.0% and on the third mating 18.0%. Total conception rate between first and third mating was 77.1%. 6. The therapeutic effect by treatment for granular vaginitis-like syndrome was 87.1%.
고광두,김두,Goh Gwang-Du,Kim Doo 한국임상수의학회 1991 한국임상수의학회지 Vol.8 No.1
A total of 2,024 quarters of 515 dairy cattle in Kangweon area were examined for incidence of subclinical mastitis. Milk samples from cattle infected with subclinical mastitis were studied bacteriologically and the bacterial strains isolated were further examined for sensitivity to 12 antibacterial agents. And the status of carrying out the mastitis control program in 28 dairy farms was examined. The results obtained were summerized as follows ; 1. A total of 308(59.8%) of 515 cattle and 656(32.4%) of 2,024 quarters were found to be infected with subclinical mastitis. 2. The 277 strains of etiological agents were isolated from 358 subclinical quarters. These were identified as Staphylococcus aureus(14.4%), other staphylococci(36.5%), Streptoccus agalatiae(8.7%), other streptococci(30.7%), Bacillus spp.(1.8%), Corynebacterium spp.(1.4%) and coliform(0.7%). 3. The 109 strains of streptococci and 141 strains of staphylococci were examined for sensitivity to 12 antibacterial agents. All the strains of streptococci were sensitive to penicillin, ampicillin and cephalothin, and they were also sensitive to erythromycin(88.1%), clindamycin(83.5%), enrofloxacin(75.2%), trimethoprim+sulfamethoxazole(67.9%), The strains of staphylococci were sensitive to cephalothin(97.2%), gentamicin(83.0%). enroflozacin(80.9%), trimethoprim+sulfamethoxazole(78.0%), erythromycin(71.6%) and clindamycin(71.6%). But all the strains resisted to colistin. 4. In the 28 dairy farms examined, condition of udder before washing was dirty in most of the farms (89.7%). Hygiene of milking equipment was only good in the 5 farms(17.9%). Teat preparation before milking was good in the 6 farms(21.4%). The farms in which teat dipping after milking was conducted were 46.4%. Dry cow treatment for the complete herd was carried out in most of the farms(89.3%) but mastitis checking was only carried out in the 8 farms(28.6%) irregularly.
김두,고광두,박춘근,김현기,Kim Doo,Goh Gwang-Du,Park Choon-Keun,Kim Hyun-Ki 한국임상수의학회 1989 한국임상수의학회지 Vol.6 No.1
This study was performed to determine the blood chemical values of the 50 normal multiparous and 98 infertile dairy cattle which were raised in the high-land of kangweon area. The results obtained in this study were summerized as follow. 1. The mean values of the serum total protein, albumin and A/G ratio the infertile dairy cattle were lower than those of the normal multiparous dairy cattle. 2. The mean value of the serum globulin of the infertile dairy cattle was higher than that of the normal multiparous dairy cattle. 3. The mean values of the serum total cholesterol, glucose, calcium and inorganic phosphorus of the infertile dairy cattle were lower than those of the normal multiparous dairy cattle. 4. The mean value of the SGOT of the Infertile dairy cattle was higher than that of the normal multiparous dairy cattle.
고지사육(高地飼育) 번식장해(繁殖障害) 유우(乳牛)의 혈액학치(血液學値)의 관(關)한 연구(硏究)
유규연,고광두,박춘권,김현기,Yoo, Kyu-yon,Goh, Gwang-du,Park, Choon-keun,Kim, Hyun-ki 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.2
Observations were made on the blood picture of 50 multiparous and 98 infertile cows. The results obtained in this study were summarized as follows. 1. Mean values of RBC, Hb, PCV, MCV, MCH, MCHC and platelets for normal multiparous cows were $6.87{\pm}0.84{\times}10^6/mm^3$, $10.3{\pm}1.3g/100ml$, $31.4{\pm}3.7ml/100ml$, $46.1{\pm}6.9{\mu}^3$, $15.3{\pm}2.6pg$, $34{\pm}2.7%$, and $372.7{\pm}304.7{\times}10^3/mm^3$ respectively. 2. Mean values of RBC, Hb, PCV, MCV, MCH, MCHC and platelets for infertile cows were $7.00{\pm}0.98{\times}10^6/mm^3$, $10.6{\pm}1.2g/100ml$, $32.1{\pm}3.6ml/100ml$, $45.8{\pm}6.7{\mu}^3$, $15.3{\pm}1.8pg$, $33.9{\pm}3.3%$, and $382.7{\pm}157.5{\times}10^3/mm^3$, respectively. 3. Mean values of WBC count for normal multiparous and infertile cows were $7.92{\pm}1.72$ and $9.04{\pm}2.87{\times}10^3/mm^3$ respectively. 4. In the differential leukocytes count, mean values of neutrophils, lymphocytes, monocytes, eosinophils and basophils for normal multiparous cows were $29.7{\pm}7.9%$, $56.8{\pm}7.6%$, $2.9{\pm}1.9%$, $9.9{\pm}5.3%$ and $0.3{\pm}0.7%$, respectively, and mean values of neutrophils, lymphocytes, monocytes, eosinophils and basophils for infertile cows were $31.9{\pm}7.8%$, $57.5{\pm}7.9%$, $2.6{\pm}1.9%$, $7.5{\pm}4.8%$, and $0.4{\pm}0.2%$, respectively.
高光斗 건국대학교 1979 論文集 Vol.9 No.1
Infertility and its occurrence of Korean native cows in Taebaek district were investigated. The results obtained were summarized as follows: ① In the breeding status of 1.540 heads of Korean native cows, pregnancy of 54.1 %, reproductive disorders of 14.7%, physiological vacancy of 23.5% and uncertain pregnancy of 7.7% were shown. ② The diseases of the genital organs in the infertile cows were 50.9% in the vaginas, 27.4% in the uteri and 21.7% in the ovaries. ③ The granular vaginitis in the vaginas of the cows was consisted of 27.8% of sverer form and 72.2% of milder one. ④ Observing the infertile cows in the age, 48.9% of them was included in the group of below 3 years old. The frequency of the infertility showed a tendency to decrease as their age increased. ⑤ The highest frequency of the infertility was 16,7% in the alpine area. ⑥ Eleven genuses of microorganisms were dected in the genital secretion of the affected cows.