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한우의 수정란 이식에 관한 연구 제3보 . 수정란의 비외과적 채취와 이식
고광두,정길생,이기만 ( Gwang Du Goh,Kil Saeng Chung,Ki Man Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4
For the purpose of obtaining the artificial twin birth in the transplanted Korean native cows, non-surgical obtaining of the fertilized eggs and their transplantations were performed. The results were as follows: 1. Average six eggs per donor were ovulated and 65% of them were recovered by non-surgical technique. 2. Eighty two percent of eggs recovered were found to be morphologically normal and were at 4-32 cell stage, respectively. 3. Seventy five percent of cattle treated with prostaglandin F₂α were synchronized in their estrus within 4 days after administration. 4. No twin fetation was obtained from the recipients which received fertilized eggs.
한우의 수정란 이식에 관한 연구 제1보 . GTH 와 PGF 2α 투여에 따른 난소반응
고광두,정길생,이기만 ( Gwang Du Goh,Kil Saeng Chung,Ki Man Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4
For the induction of follicle development and heat, PGF₂α was injected intramuscularly two days after PMS was injected by subcutaneous or intra-ovary methods on multiparous cows during of 9-13 days of the estrus cycle. After the above treatments, intravenous HCG injection for the superovulation was followed. The responses of ovaries were as follows: 1. Average duration from PMS administration to the onset of estrus was 5 days and the duration was shortened with the increasing dosage of PMS. 2. Duration of induced estrus was ranged from 2.3 to 4.8 days and the duration of estrus was shortened with the decreasing dosage of PMS. 3. Number of follicle obtained from the cattle treated with 4,000, 3,000, 2,000, 1,000 and 500 IU of PMS were clarified to be 11.4, 11.5, 7, 2.3 and 2.5, respectively, after 4.8 - 5.2 days of PMS administration. 4. Observing the results of slaughter, ovulation rates of the cattle treated with 4,000, 3,000, 2,000, 1,000, and 500 IU of PMS were 16.3, 35.6, 60.0, 65 and 75%, respectively. 5. The numbers of degenerated, or bled follicle were increased with the increase of PMS dosage.
한우의 수정란 이식에 관한 연구 제1보 . GTH 단독투여에 의한 한우의 쌍태 유기
고광두,정길생,이기만 ( Gwang Du Goh,Kil Saeng Chung,Ki Man Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4
For the studies of twin birth induction on Korean native cattle, the following processes were taken: first, PMS were injected subcutaneously to promote the growth of follicles, then HCG were injected into the vein for the superovulation and the artificial insemination were performed for conception. The results were gained as follows: 1. Average duration from PMS administration to onset of estrus was 3.8 to 4.7 days and no differences due to dosage of PMS were observed. 2. Duration of induced estrus was ranged from 3.3 to 4.6 days and the length of induced estrus cycle was shortened than that of normal estrus. 3. The most grown follicles (5.3 ∼ 5.5) were observed in the cattle administrated with 2,000 IU of PMS and 1,000 IU of PMS was considered to be the optimum dosage for the limited ovulation. 4. Ovulation rate was declined when the PMS dosage was increased more than 1,000 IU. 5. The highest rate of twin birth (35.7%) was obtained from the cattle administrated with 1,000 IU of PMS and the next was the cattle administrated with 2,000 IU and 750 IU of PMS, respectively.