Effects of Histamine, 5-Hydroxytryptamine and Their Antagonists on the Uterine Motility in the Rat

유규연,Yoo, Kyu-yon The Korean Society of Veterinary Science 1988 大韓獸醫學會誌 Vol.28 No.1

평골근(平滑筋)으로 된 자궁근(子宮筋)은 자동성(自動性)을 가지고 있어서 신경지배(神徑支配)와 관계(關係)없이 근자체(筋自體)로 운동(運動)을 하게 된다. 그러나 이러한 자궁근(子宮筋)의 형태적(形態的) 및 기능적(機能的) 정상상태유지(正常狀態維持)에는 estrogen의 작용(作用)이 불가결(不可缺)한 요소(要素)로 되어 있으며 이 estrogen의 작용(作用)에 의하여 histamine의 자궁근(子宮筋)에 대한 (작용)作用이 수용체(受容體)의 어떤 기전에 의한 것인지를 알기 위하여 본(本) 연구(硏究)는 histamine과 5-hydroxytryptamine 및 이들 길항물질(拮抗物質)들의 자궁근(子宮筋) 운동성(運動性)에 대한 수축(收縮) 및 이완작용(弛緩作用)을 조사(調査)하였다. 자궁근(子宮筋)의 운동성(運動性)은 physiograph를 통(通)해 자궁수축(子宮收縮)의 빈도(頻度)와 크기를 기록하여 아래와 같은 결론(結論)을 얻었다. 1. 5-hydroxytryptamine에 대한 phenoxybenzamine의 억제작용(抑制作用)은 phenoxybenzamine의 길항성(拮抗性)의 결과(結果)이다. 2. histamine은 $H_1$-receptor를 통해서 흰쥐의 자궁평활근(子宮平滑筋)의 운동성(運動性)은 증가(增加)한다. 3. 반면 histamine은 $H_2$-receptor를 통해서는 자궁평활근(子宮平滑筋)의 운동성(運動性)을 이완(弛緩)시켰다. 4. 흰쥐의 자궁근(子官筋)에서 $H_2$-receptor 차단제(遮斷劑)가 $H_1$-receptor 차단제(遮斷劑)의 작용(作用)보다 더욱 강하였다.

고지사육(高地飼育) 번식장해(繁殖障害) 유우(乳牛)의 혈액학치(血液學値)의 관(關)한 연구(硏究)

유규연,고광두,박춘권,김현기,Yoo, Kyu-yon,Goh, Gwang-du,Park, Choon-keun,Kim, Hyun-ki 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.2

Observations were made on the blood picture of 50 multiparous and 98 infertile cows. The results obtained in this study were summarized as follows. 1. Mean values of RBC, Hb, PCV, MCV, MCH, MCHC and platelets for normal multiparous cows were $6.87{\pm}0.84{\times}10^6/mm^3$, $10.3{\pm}1.3g/100ml$, $31.4{\pm}3.7ml/100ml$, $46.1{\pm}6.9{\mu}^3$, $15.3{\pm}2.6pg$, $34{\pm}2.7%$, and $372.7{\pm}304.7{\times}10^3/mm^3$ respectively. 2. Mean values of RBC, Hb, PCV, MCV, MCH, MCHC and platelets for infertile cows were $7.00{\pm}0.98{\times}10^6/mm^3$, $10.6{\pm}1.2g/100ml$, $32.1{\pm}3.6ml/100ml$, $45.8{\pm}6.7{\mu}^3$, $15.3{\pm}1.8pg$, $33.9{\pm}3.3%$, and $382.7{\pm}157.5{\times}10^3/mm^3$, respectively. 3. Mean values of WBC count for normal multiparous and infertile cows were $7.92{\pm}1.72$ and $9.04{\pm}2.87{\times}10^3/mm^3$ respectively. 4. In the differential leukocytes count, mean values of neutrophils, lymphocytes, monocytes, eosinophils and basophils for normal multiparous cows were $29.7{\pm}7.9%$, $56.8{\pm}7.6%$, $2.9{\pm}1.9%$, $9.9{\pm}5.3%$ and $0.3{\pm}0.7%$, respectively, and mean values of neutrophils, lymphocytes, monocytes, eosinophils and basophils for infertile cows were $31.9{\pm}7.8%$, $57.5{\pm}7.9%$, $2.6{\pm}1.9%$, $7.5{\pm}4.8%$, and $0.4{\pm}0.2%$, respectively.

肉鷄의 抗生物質 殘溜量 調査

劉圭然 ( Kyu Yeon Yoo ),金泰鎬 ( Tae Ho Kim ),李榮澤 ( Young Taik Lee ),金昌秀 ( Chang Soo Kim ),金鍾冕 ( Jong Myeon Kim ) 국군의무사령부 1980 대한군진의학학술지 Vol.11 No.1

A STUDY ON THE FERTILITY REGULATION METHODS BY THE POINTS OF INTERVENTION IN THE REPRODUCTIVE PROCESSES : from Germ cell to Implantation

Park, Sang Hwa,Yoo, Kyu Yun,Kim, Eung Ik 대한보건협회 1987 대한보건연구 Vol.13 No.2

One hundred ninety-seven cases of decompression sickness who were treated at Kangnam General Hospital Hyperbaric Unit for one year are reviewed. One hundred seventy-two patients(87.3%) had pain only. The other 25 patients(12.7%) had more serious decompression sickness. More than 90% of the patients had been shellfishing diving. In April-May, 1987, a survey was conducted of diving fishmen, 82 of whom were selected for this study with questionnaire. The ages of the divers ranged from 25 to 47 with a mean 30,8 years. On an average day they made from 3 to 7 dives with a mean bottom time 27.5 minutes. The divers ranged in depth from 30 to 50 meters. Ninety-two percents of respondents had had one or more incidents of type Ⅰ decompression sickness which, they had treated by immediate in-water recompression. Forty-four divers(53%) had experienced one or more incidents of more serious type Ⅱ decompression sickness including neurological involvement.

흰쥐 자궁 및 착상에 있어서 Tamoxifen과 성스테로이드 호르몬의 상호관계

한호재,유규연,권종국,양일석,Han, H.J,Yoo, K.Y.,Kwun, J.K.,Yang, I.S. 대한생식의학회 1988 Clinical and Experimental Reproductive Medicine Vol.15 No.2

These studies were undertaken to examine the relationship between tamoxifen and sex steroid hormones in rat uterine morphology and the effect of tamoxifen on sex steroid hormone levels, implantation and myometrial contraction. The results obtained were as follows : 1) The increase in height of the luminal epithelium caused by tamoxifen treatment was blocked by progesterone. The increase in height of luminal epithelium caused by $estradiol-17{\beta}$ treatment was blocked by tamoxifen. 2) When a single dose of tamoxifen(10, 20, $40{\mu}g$) was given on Day 2 of pregnancy, implantation was prevented. Plasma $estradiol-17{\beta}$ level fell in a dose-dependent manner but plasma progesterone level was constant. 3) In vitro, tamoxifen decreased rat uterine contractility in a dose-dependent manner.

韓國人 血淸 Pseudocholinesterase 活性水準과 肥滿度에 關한 硏究

王貞美,韓政浩,劉圭然,金應翊 대한보건협회 1990 대한보건연구 Vol.16 No.1

Obesity is a major health problem for many countries because it is so prevalent and the association of overweight with many serious physical impairment is evident from numerous studies. Therefore, research efforts toward elucidation of biological markers for obesity as an effective strategy for prevention will have public health importance. The objective of this study was to determine the serum pseudocholinesterase activity levels by sex, age, weight, obesity index and weight change, and also to determine the usage of serum PchE activity as early detection marker of obesity. Serum PchE activity, weight and height data were measured in 710 healthy adult subjects (533 male, 177 female) and the major results of this study are as follows; 1. The mean of serum PchE activities for male subjects was 6.52±1.37 u/ml which was significantly higher than that of female subjects(5.72±1.20 u/ml, p<0.001). The range of serum PchE activity was 2.84 u/ml to 14.09 u/ml for males and 3.52 u/ml to 10.43 u/ml for females. 2. The average age of male subjects was 40.2 years old and that of female subjects was 34.8 years old. In males, the mean of serum PchE activities in each age group appeared statistically different (p<0.05), but this difference seems to be related to weight by sex. 3. There appeared no significant difference in the mean of serum PchE activities in each height group (male p>0.6, female p>0.6) and the correlation coefficient was very low in both sexes with 0.0070 and -0.0706 respectively for males and females. 4. A significant incease in the mean serum PchE activity was found in subjects groups on the basis of the weight in both sexes by one-way varience analysis (male p<0.001, female p<0.05). But the correlation coefficient between serum PchE activity and weight was relatively low in both sexes as 0.1965 and 0.2687 respectively. 5. Serum PchE activity increased as the degree of obesity increased in both sexes. We found that the difference of the means of serum PchE activities in each obesity group was significantly high(male p<0.001, female p<0.001). Although the degree of obesity didn't show good correlation with serum PchE activity in both sexes(male 0.2381, female 0.2939), but this correlation coefficient was higher than that of between weight and serum PchE activity. 6. The difference of mean serum PchE activity by weight changes from 1984 to 1988 was statistically significant in each weight change group (p<0.005), but the correlation coefficient was very low (male 0.0999, female 0.1168). The result of a t-test in each group showed that the difference of mean serum PchE activity was significant only between decreasing weight group and the other groups (p<0.01). 7. Although we found that the difference of mean serum PchE activity by weight, obesity and weight changes was statistically significant, the relatively low values of correlation coefficient indicate that the serum PchE activity may not be a useful marker in predicting the risk for obesity.

개의 바이러스성(性) 장염(腸炎)의 국내발생례(國內發生例) 보고(報告)

한홍율,황의경,유규연,이영옥,Han, Hong-Ryul,Hwang, Eui-Kyung,Yoo, Gyu-Yeon,Rhee, Young-Ok 대한수의학회 1982 大韓獸醫學會誌 Vol.22 No.2

An acute enteric diseases accompanied with vomiting, diarrhea, anorexia, pyrexia, dehydration and high mortality has occured in dogs throughout the country since June, 1981 in Korea. According to epidemiological and clinico-pathological studies on the total of 44 dogs admitted to the animal clinic in the college, the cases were tentatively diagnosed as a viral enteritis and the possibility of canine parvovirus as the etiological agent for the cases was discussed.

PMSG 전처리한 쥐에 있어서 Antiprogesterone(RU486)과 Antiestrogen(Tamoxifen)이 배란과 Oocyte에 미치는 영향

윤영원,권종국,유규연 韓國受精卵移植學會 1989 한국동물생명공학회지 Vol.4 No.1

The effects of an antiprogesterone (RU 486) and an antiestrogen (tamoxifen) on ovulatory response and oocyte morphology were examined in pregnant mare serum gonadotropin (PMSG)-primed immatare female rats (28 days of age): a comparison has been made on two different regirnens primed with a "control" dose (4 IU) and a "superovulatory" dose (40 IU) of PMSG. Females for control control regimen received three consecutive injections of lmg RU486, lmg tamoxifen, or vehicle at 24, 36 and 48hr, and were killed at 72l'r after PMSG. Animals for superovalatory regimen received lmg RU486, 2.5mg tamoxifen, or vehicle fouowlag the injection schedule comparable to control regimen, and were killed at 60 and 72hr after PMSG. Compared to vehicle group, there was a significant reduction in ovulatory response as judged by the proportion of rats ovulating andi or by the mean number of oocytes per rat for each treatment of RU486 and tamoxifen in both regimens. The activity of tamoxifen in inhibiting the ovulatory response was greater in control, but less in superovulatory regimen than that of RU486 based on the dose employed for each antisteroid. In both regimens, RU 486 did not have any effect 6n the changes in the proportion of degenerate oocytes as well as ovarian weight, well tamoxifen treatment resulted in a marked promotion of oocyte degeneration as well as a great reduction in ovarian weight, compared to each parameter of vehicle group. RU486 treatment in each regimen did not alter the serum levels of any steroid hormones observed. Howerver, tamoxifen treatment was associated with significant increases in serum 17-estradiol and decreases in progesterone in both regimens; also significant increases in androgens in superovulatory regimen. The results illustrate the relative inhibitory activity of RU486 and tamoxifen indicating major steroid hormone involved in PMSG-induced ovulation: 17-estradiol for control and progesterone for superovulatory regimen. It also appears that tamoxifen-associated elevation of circulating 17-estradiol andi or androgens could be in part, a contributing factor to the promotion of oocyte degeneration presumably by producing a hostile oviductal environment after ovulation.ent after ovulation.

Rat에서의 Ketamine, Xylazine 및 Droperidol-fentanyl 마취

남치주(Tchi-Chou Nam),유규연(Kyu-Yon Yoo) 한국실험동물학회 1986 Laboratory Animal Research Vol.2 No.1

개 파보바이러스성(性) 장염(腸炎)의 국내발생(國內發生)

이영옥,최대영,박봉균,한홍율,황의경,유규연,Rhee, Young-Ok,Choi, Dae-Young,Park, Bong-Kyun,Han, Hong-Ryul,Hwang, Eui-Kyung,Yoo, Gyu-Yeon 대한수의학회 1982 大韓獸醫學會誌 Vol.22 No.2

The disease syndrome characterized by the acute vomiting and diarrhea with high mortality had been greatly epidemic in Korea since June, 1931 and it was followed serologically and electron microscopically for the clarification of the agent. The agent present in feces of dogs associated with this syndrome had characteristic feature in agglutinating pig red blood cells that was specifically inhibited by anti-CPV reference dog serum. This also showed the serological identity with the reference CPV antigen in immuno-diffusion. Electron micrograph of the material revealed parvovirus particles with size of 20nm and icosahedral structure. These results clearly indicated that CPV was the primary cause of canine epidemic prevailing in 1981 in Korea.