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      • KCI등재

        산화적 스트레스에 의한 간세포의 DNA 손상 및 세포사멸 유도에 미치는 원지 에탄올 추출물의 보호 효과

        김홍윤,박철,최영현,황원덕 한방비만학회 2019 한방비만학회지 Vol.19 No.1

        Objectives: The purpose of the present study was to evaluate the preventive effects of ethanol extract of Polygalae radix (EEPR) against oxidative stress (hydrogen peroxide, H2O2)-induced DNA damage and apoptosis in Chang liver cells. Methods: Chang liver cells were pretreated with various concentrations of EEPR and then challenged with 0.5 mM H2O2. The cell viability and apoptosis were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry analysis, respectively. The levels of reactive oxygen species (ROS), mitochondrial membrane potentials (MMPs) and adenosine tri-phosphate (ATP) contents were measured. Expression levels of Bcl-2 and Bax were also determined using Western blot analysis. Results: The results showed that the decreased survival rate induced by H2O2 could be attributed to the induction of DNA damage and apoptosis accompanied by the increased production of ROS, which was remarkably protected by EEPR. In addition, the loss of H2O2-induced MMPs and ATP contents was significantly attenuated in the presence of EEPR. The inhibitory effect of EEPR on H2O2-induced apoptosis was associated with up-regulation of Bcl-2 and down-regulation of Bax, thus reducing the Bax/Bcl-2 ratio. Conclusions: Our data prove that EEPR protects Chang liver cells against H2O2-induced DNA damage and apoptosis by scavenging ROS and thus suppressing the mitochondrial-dependent apoptosis pathway.

      • 動力耕耘機의 機種別 耕耘碎土作業 性能에 關한 硏究

        金鴻允 안성산업대학교 1979 論文集 Vol.11 No.-

        動力耕耘機의 機種別 耕耘作業性能에 關한 試驗을 實施하기 爲하여 3.5p.s小型, 5p.s中型, 8p.s大型, 耕耘機를 供試機로 하여 쟁기耕耘作業과 Rotary??土作業을 實施하여 본바 ① 一般的인 耕耘, ??土作業에서는 5p.s의 中型耕耘機가 가장 便利하고 能率的인 機種으로 밝혀졌고 ② 大規模, 深耕, 耕耘을 爲하여는 大型耕耘機가 有力한 것으로 나타났으며 ③ 小規模, 不均衡耕地에서 幼弱盧動吸收나 作業便易를 爲하여서는 小型動力耕耘機가 便利한 機種으로 結論되었다. This experiment has been Carried out to investigate plowing and harrowing operation by the 3.5 PS. power, the 5P.S power, the 8P.S power tiller. The results are summarized as follows, The plowing time by the 8P.S tiller needed about 1.5 hours per 10a area, the 5P.S tiller about 1.7hours, but the 3.5 P.S tiller needed more then 3hours per 10a area, Too, the harrowing time by the 8P.S, 5P.S Power tiller needed about 0.5 hours per 10a, but the 3.5P.S tiller needed more then 1.5 hours. ① The 5P.S power tiller Could be easily operated to plowing and harrowing at the general fields, ② The 3.5P.S miniature power tiller Could be needed to plow at special Small size area ③ The 8P.S power tiller Could be needed to depth plowing at long size area.

      • 碎土 移秧沓 土壤硬度 測定에 關한 硏究

        金鴻允 안성산업대학교 1986 論文集 Vol.18 No.-

        써래질한 논에서 土壤硬度를 測定할 수 있는 落下式 圓錐型 土壤硬度器는 設計 製作(6種)하여 이를 供試콘(cone)으로 한 1,2次 基礎實驗을 通하여 2個의 供試콘을 選定한 다음, 이로써 全國 5個地域, 56個 移秧畓에서 碎土作業 直後와 移秧當時의 土壤硬度를 콘의 貫入深으로 調査하여 다음과 같은 結果를 얻었다. (1) 1次實驗에서 4種의 供試콘을 만들어 畓土壤을 3類型의 土塊土壤으로 만들어 콘의 貫入深을 比較 測定한 結果로 A,B,C콘 사이에는 差異가 없었고, D콘만이 有意差가 있었다. (2) 2次實驗에서는 6種의 供試콘으로 畓土塊를 碎土 整地한 狀態에서 콘의 貫入深을 比較 分析한 結果 D콘만 差異가 있었고 나머지 A,B,C,D,E,F콘사이에는 有意茶가 없어서 供試콘간에 變化가 적은 B콘과 變化가 심한 F콘을 선정하였다. (3) 現地 調査地域의 移秧畓에서 土壤塊度는 碎土作業直後 12.4∼13㎝인 것을 平均 약 1.8㎝ 沈地시켜 移植作業을 할 때에는 10.7∼11.2㎝로 된다. (4) 調査地域의 移秧畓에서 碎土作業은 94.6% 以上이 動力耕耘機 로우터리에 依存하고 있으며 機械移秧作業도 53.6%나 된다. (5) 以上의 結果로 써래질한 논의 土壤硬度를 測定하기 위하여 本 試驗에서 製作 選定된 콘이 有用할 것이다. It has an important effect on the rice transplanting operation that the paddy field soil has a fine puddling condition. In order to measure the soil hardness for the farmer's puddling fields, 6 falling type soil hardness testing cones were designed. Through first and second basic test, two cones were selected. The two cones were used to measure that soil hardness on puddings depth of the falling cone. The results are summarized as follows: 1) Among the 4 sample cones (A, B, C and D type), the first 2 types were recommended for hardness tester for the puddling soil through the first basic test. 2) All sample cones, except D-type, were recommended for hardness tester for the pudding soil through the second basic test. Finally the B and F-type sample cone were recommended to measure the soil hardness for the puddling fields over the investigated regions. 3) The soil hardness, on the pudding and transplanting day, showed a penetrating depth of 12.4 to 13cm 10.7 to 11.2cm each. The transplanting operations were taken after the puddling soil hardened to decrease the penetrating depth by 1.8cm. 4) In the sample regions, the mechanical transplanting area took 53.6% and the puddling operation by power and tractor was 94.6%. 5) The cone selected in this test will be useful measuring the puddling soil hardness in the paddy field.

      • KCI등재

        A control strategy for electro-magneto-mechanical system based on virtual system model

        김홍윤,윤영민,허훈 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.9

        A new approach to the control of electro-magneto-mechanical system is proposed in this paper. Conventionally, these systems are controlled based on the Maxwell system model via an on-off or PID control technique, which displays acceptable performance in the low frequency region, but not in the high frequency region where position control performance is greatly degraded. In order to improve the performance, a newly developed virtual 2 nd order system modeling technique, SSID, is adopted for a complex electro-magnetomechanical system in the study. This technique states that any unknown system exposed to a random disturbance with unknown intensity can be identified in terms of a virtual 2 nd order system model via the inverse process of a certain stochastic analysis. As a typical hybrid system, a solenoid valve is used as the target electro-magneto-mechanical system to study the modeling of the virtual 2 nd order system. In order to confirm the performance of the proposed control strategy, autotuning PID controller in PWM mode is utilized. Simulations based on the conventional Maxwell system model with control via the bang-bang, autotuning PID, and the proposed virtual 2 nd order system model approaches are conducted using MATLAB Simulink. Performance of these three systems in the low and high frequency bands is also compared. The simulation results reveal that the control performance of the virtual 2 nd order system model is much improved compared with that of the Maxwell system model under autotuning PID and bang-bang controls in both low and high frequency regions, where the error is drastically reduced to approximately 1/5 of the original value.

      • KCI등재

        허브부식토 급여가 비육우의 증체 및 육질개선에 미치는 영향

        김홍윤,박중국,안종호,Kim, Hong-Yun,Park, Joong-Kook,Ahn, Jong-Ho 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

        본 연구는 허브부식토 급여가 비육우의 증체 및 육질개선 효과를 조사하기 위해 대조구, 허브부식토제제 첨가구(허브초탄, T1), 코팅 비타민 C제제 첨가구(Vit. C, T2) 및 코팅 비타민 C제제와 허브부식토제제 혼합 첨가구(허브초탄+Vit. C, T3)로 나누고 공시동물은 각 시험구당 5두씩 임의 배치하여 수행하였다. 개시체중은 대조구, T1, T2 및 T3구에서 각각 $689{\pm}31$, $661{\pm}24$, $659{\pm}32$ 및 $622{\pm}19kg$이었으며, 종료 체중을 계산한 일당증체량은 T3구에서 대조구와 비교하여 8.3% 높았으며, T1 및 T2구에서도 대조구와 비교하여 약 2.9% 높았다. 실험 경과 후 BUN은 T2구에서 T3구와 비교하여 유의하게 높게 나타났으나(p<0.05), glucose, AST, ALT 및 총 단백질 함량은 차이가 없었다. 도체등급 판정에 의한 도체중, 등지방두께, 근내지방, 등심단면적, 육량지수 및 육질등급을 조사하였으나 처리구간 유의한 차이는 없었다. 그러나 등심의 조지방 함량은 T1, T2, 및 T3에서 각각 9.92, 14.69 및 13.00%로 T2구에서 대조구 및 T1구보다 높게 나타났다. 도체의 지방산 조성은 linoleic acid(C18:2n6) 경우 대조구와 비교하여 T3에서 유의하게 낮은 경향을 보였지만, ${\gamma}$linoleic acid(C18:3n6)은 T3구에서 다른 처리구와 비교하여 가장 높은 함량은 나타냈다. 전반적인 실험의 결과를 종합하여 볼 때 부식토 급여가 육질 등급을 다소 개선하는 경향이 있었으나, 육량 및 육질 향상을 위한 비육 전기간 부식토를 급여하는 연구가 추 후 이루어져야 할 것으로 판단된다. The experiment was conducted to investigate the effects of feeding herbaceous peat on growth performance and meat quality of Holstein beef cattle. Total of 20 Holstein beef cattle (18~20 month of age, $657{\pm}31kg$ body weight) were conventionally and separately fed a concentrate diet and rice straw for 134 days. The dietary treatments were randomly assigned by complete block design into four treatments, each of which were five heads in early fattening stage. The treatments in this study were the control group fed basal diet, feeding herbaceous peat group (5%/diet, T1), feeding coated vitamin C group (20g/head, T2) and feeding mixture of herbaceous peat and coated vitamin C group (5%/diet+20g/head, T3). The initial body weights between the groups of control, T1, T2 and T3 were similar showing with $689{\pm}31$, $661{\pm}24$, $659{\pm}32$ and $622{\pm}19kg$. The daily body weight gain was higher in T3 by 8.3% than that in the control (p<0.05). Glucose concentration in control group was the highest among treatments (p<0.05), but there was no significant differences between treatments on AST (aspartate aminotransferase), ALT (alanine aminotransferase), BUN and total protein concentrations of blood. The fat content of sirloin in the T2 was significantly higher than control and T1 group (p<0.05). Meat color (CIE) values in T2 was the highest among treatments (p<0.05), and other treatments also increased those values. In overall, the feeding herbaceous peat and vitamin C to the Holstein beef cattle was considered to have positive effects on the growth performance of Holstein beef cattle. In addition, the effects on the performances of animals were more improved when fed herbaceous peat and vitamin C concurrently.

      • 沓 碎土作業別 콘의 貫入深과 作物收量

        金鴻允 안성산업대학교 1987 論文集 Vol.19 No.-

        畓碎土作業을 트랙터나 動力耕耘機用 로터리를 使用하여 5種의 碎土作農方式을 處理하고 各處理區 間에 콘(Falling cone)의 貫入深과 作物 收穫量을 比較 分析한 結果 다음과 같은 結論를 얻었다. (1) 畓碎土作業에서 理論上 土塊크기가 클수록 콘의 貫入深은 작게 나타난다. (2) 畓碎土作業에서 土塊沈下現象은 三期로 나누어진다. 碎土作業後 初期는 搖動沈下期間으로 不規則的인 垂直沈下가 일어나며, 中期인 安靜沈下期間은 規則的인 垂直沈下가 일어나고 末期인 停止沈下期間에서는 서서히 沈下現象이 停止되어 간다. (3) 畓碎土作業時에 耕土層의 中層部만 微細하게 碎土하면 搖動沈下期間이 길게 되고 中層部位가 含水密閉層으로 長期間 流動性을 維持하게 되어 作物뿌리 發育에 惡影響을 미칠것으로 判斷된다. In order to mechanize rice transplanting in paddy field, it is very important to search the adaptable pulverization in the paddy soil by tractor rotary or power tiller. This study was conducted to measure the penetrating depth by the falling cone at pulverizing paddy soil which was puddled by rotary of tractor and power tiller, and to investigate the yields in the testing field which was operated to all same conditions. 1. The penetrating depth by falling cone decreased at rough pulverizing paddy soil which the theoretical cutting soil constituted to more big grains than small. 2. It suggested that the sinking state of puddling soil grains appeared to change three stages period: the first stage is shaking sedimental period, the second stage is quiety sedimental period , the third stage is rest sedimental period. 3. If you are operated puddling paddy soil (treatment C in this test) by tractor rotary or power tiller in order to get pulverization of small grains in which in medium stage in the tillage soil, I will be supposed that the shaking sedimental period is maintained for long time and included the more amount of gas than the other puddling paddy soil, that may be hindered the growth of plants root and its yields.

      • 신농촌개발을 위한 농학,공학적 정주생활권 모형의 개발(II) - 토지이용 계획 -

        김홍윤,이신호,이홍주,전우형,정예표,조흥수,김영철 한국농공학회 1993 韓國農工學會誌 : 전원과 자원 Vol.35 No.3

        For a systematic approach to rural settlement planning, socio-economic and land use potentialities were analysed in 10 villages("Ri", the lowest-order administrative unit in Korea)of Izuk-myun, Anseong-gun, Kyeonggi province, the model area in this study. Socio-economic potentialities, sub-grouped into rural and urban related ones, were analysed by the principal component analysis technique, while land use potentialities by the land suitability system of which the physical analysis is based on geographical information system. The principal component of rurality is strongly related with 5 key variables such as annual increasing rate of farm households, ratio of over 1ha-households, ratio of full-time farmers, ratio of animal rearing households and the principal component of urbanity with 6 key ones such as population density, number of schools, number of shops and servicing facilities, number of daily bus routes, number of non-farm households, percentage of area of housing sites. The analysis procedure of land suitability using the geographical information system were generalized and the results of analysis on village sites and paddy and upland fields were presented. The whole land use planning was presented by the criteria of the land suitability rank and the priority order of land use. land use.

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