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禹永國,權純映,劉成桓 관동대학교 1997 關大論文集 Vol.25 No.2
The combined process for denitrification was used to treat seafood processing wasteweters containing high Nitrogen compounds. The reactors were maden by prexiglass and consisted of the two anoxic and oxic reator for nitrification and denitrification. Wastewater for experiment was ranged in 4000-5000㎎/1 of TCOD, 2500-3300㎎/1 of TBOD, 500-600㎎/1 of TKN, 430-500㎎/1 of NH₃-N and 14-28℃ of temperature. The efficiency was shown in treating nitrogen compounds during experiment. About 90% of NOx was removed at anoxic oxic phase. At the state of 0.3-0.6 ㎏ NOx/m³.d in Nitrogen volumetric loading rate, the efficiency was 97%. Most of Nitrogen removal was treated at the post-denirification.
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류성환,전흥우 金烏工科大學校 1998 論文集 Vol.19 No.-
In this paper, the digitally programmable 6×6 cellular neural network chip is designed and the circuit operation is confirmed by the HSpice simulation of edge detection and hole filling. The designed cellular neural network allows for increased flexibility compared to the analog tuning mode due to the fact that synapse weights can be both programmed and stored digitally. The network considered in this design is a current mode cellular neural networks. Since the number of different weights is very small for this cellular neural networks. the programmability issues can be easily incorporated by just adding several global control lines. And the current mode circuit also provides the simplicity over voltage mode circuit.
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운동과 태음조위탕 처방이 흰쥐의 간기능 효소와 간내 지방축적에 미치는 영향
윤진환,류성환,정경훈,최덕구,정일규,이희혁,김종오,정진욱 한국운동과학회 2002 운동과학 Vol.11 No.2
윤진란, 류성환, 정경훈, 최덕구, 정일규, 이희혁, 김종오, 정진욱. 운동과 태음조위탕 처방이 흰쥐의 간기능 효소와 간내지방축적에 미치는 영향. 운동과학, 제11권 제2호, 345-358, 2002. 본 연구는 태음조위탕과 운동처방이 비만치료에 생리학적, 조직학적으로 미치는 영향에 중점을 두고 흰쥐 체중, 효소활성도를 비롯해 인체내 대사가 가장 활발한 기관인 간의 조직을 살펴봄으로서 비만치료법으로서의 효과에 대해 알아보기 위하여 실시하였다. 본 연구결과는 다음과 같다. 운동+태음조위탕군이 다른 그룹(운동군, 태음조위탕 처방 군, 대조군) 보다 체중의 증가가 가장 낮게 나타났으며, 태음조위탕군과 운동군의 비교에 있어서는 운동군에 있어서 체중의 증가가 더 낮게 나타났다. 간 무게는 운동+태음조위탕군이 다른 그룹(운동군, 태음조위탕 처방 군, 대조군)에 비해 가장 낮았으며 운동과 태음조위탕군의 비교에 있어서도 운동군의 간무게가 더 낮음을 알 수 있었다. 총 콜레스테롤, LDL-C, 중성지방, Al의 수준은 운동+태음조위탕군이 다른 그룹(운동군, 태음조위탕 처방 군, 대조군)에 비해 더 낮게 나타났으며{각각 [F(3.36)=3.190 P=.035], [F(3.36)=123.544 P=.000], [F(3.36)=565.624 P=.000], [F(3.36)=193.935 P=.000]}, HDL-C는 운동군, 태음조위탕 처방 군, 대조군에 비해 운동+태음조위탕군이 더 높게 나타났다[F(3.36)=327.767 P=.000]. 혈중 GOT와 GPT수준은 운동군, 태음조위탕 처방 군, 대조군에 비해 운동+태음조위탕군이 낮게 나타났다. Hematoxylin-eosin은 간세포질 모두를 염색하는 방법으로 본 연구에서는 세포질 구성물질 정도가 대조군>태음조위탕 처방군>운동군>운동+태음조위탕 처방군 순으로 나타났다. Sudan Black B는 간세포내 지방성분을 알아보기 위하여 행해지는 염색법으로 본 연구에서는 다음과 같이 지방성분 함량이 대조군>태음조위탕 처방군>운동군>운동+태음조위탕 처방군 순으로 나타났다. PASS 염색을 실시한 결과 당함량이 대조군에서 가장 많고 태음조위탕 처방군>운동군>운동+태음조위탕 처방 군 순으로 나타났다. 이상과 갈이 태음조위탕과 운동의 처방은 간기능에 부정적 영향을 주지 않고 간내 지방 축적을 감소시켜 비만치료에 효과가 있는 것으로 나타났다. Yoon, J.H, Ryu, &H, Chung KK, Choi, D.G., Jeong I.G, Lee, H.H., Kim, J.O., Chung J.W. Effects of Taeyeumjoweetang and exercise administration on enzymes and fat accumulation of the liver tissue in rats. Exercise Science, 11(2): 345-358, 2002. Taeyeumjoweetang is associated with improving liver function as wall as improving lipid profile. These factors are known to reduce body fat mass in re Taeyeum person'. Exercise also can improve liver function in patients with fatty liver. The purpose of this study was to investigate the effects of both Taeyeumjoweetang and exercise on enzymes and fat accumulation in rat's liver cells. 40 Sprague-Dawley rats(14weeks old)were randomly assigned into four group; the control group(n=10), the herb tea group(n=10), the exercise group(n=10) and the herb+exercise group(10). The herb tea group were given the consumption of 50㎖ of the herb tea a day for 8weeks. The exercise protocol consisted of treadmill running for 8 weeks. The data fof TC, LDL, HDL and Al were measured in plasma Wood, and GOT and GPT were measured in liver tissue. An alpha level of .05 was used as the criteria for significance. The Obtained results are as follows: 1. There were significant differences in body weight among three groups. The herb+exercise group was lower significantly than other group. 2. There were significant differences in liver weight among three groups. The herb+exercise group was lower significantly than other group. 3. There were significant differences in TC, LDL and Al among three groups. The herb+exercise group was lower significantly than other group. But HDL levels in the herb+exercise group was highly significantly than other group. 4. There were significant differences in GOT, GPT among three groups. The herb+exercise group was lower significantly than other group, showing lowest liver cell damage. 5. In composition material quantity of liver' cell cytosol shown by Hematoxylin-eosin stain, The herb+exercise group was lower significantly than other group. 6. As results of lipid and glucose stain, The herb+exercise group was lower significantly than other group in fat accumulation and glucose levels. These findings demonstrate that both the herb tea and exercise have an effect to reduce fat and glucose accumulation but exercise effects is higher than these of the herb tea. Also these effects is highest brought about by the herb+exercise.
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12주간 태음조위탕 투여가 흰쥐의 간기능 효소와 간내 지방축적에 미치는 영향
윤진환,류성환,정경훈,최덕구,정일규,이희혁,김종오,임은미 한국운동과학회 2002 운동과학 Vol.11 No.1
윤진환, 류성관, 정경훈, 최덕구, 정일규, 이희혁, 김종오, 임은미. 12주간 태음조위탕 투여가 흰쥐의 간기능 효소와 간내 지방축적에 미치는 영향. 운동과학, 제11권 제1호, 53-65, 2002.본 연구는 태음조위탕의 비만 치료 효과에 대한 생리적인 결과뿐만 아니라 간 조직의 형태 변화에 중점을 두고 흰쥐 체중, 효소활성도틀 비롯해 인체내 대사가 가장 활발한 기관인 간의 조직을 살펴봄으로써 태음조위탕이 비만치료에 미치는 효과를 알아보기 위하여 시행되어졌다. 12주간 태음조위탕를 투여한 결과 다음과 같은 결론을 얻었다. 1) 태음조위탕을 복용한 군이 대조군에 비해 체중의 증가가 현저하게 낮았으며[F_(3,16)=l1324.047 P=.000], 간 무게에 있어서도 태음조위탕을 복용한 군이 대조군에 비해 약간 낮은 것으로 나타났다. 2) 총 콜레스테롤, LDL-Cholesterol, 중성지방, Al의 수준과 동맥경화지수는 대조군에 비해 태음조위탕을 복용한 군이 더 낮게 나타났으며{각각 [F_(3,16)=18.003 P=.001], [F_(3,16)=2456.437 P=.000], [F_(3,16)=413.735 P=.000], [F_(3,16)=465.003 P=.000]}, HDL-Cholesterol은 대조군에 비해 태음조위탕 복용군이 더 높게 나타났다[F_(3,16)=2195.873 P=.000]. 3) 혈중 GOT와 GPT수준은 대조군과 태음조위탕을 복용한 군간에는 유의한 차이는 보이지 않았으나 태음조위탕을 복용한 군에서 약간 낮게 나타났다. 4) Hematoxylin-eosin으로 염색한 결과 실험군의 간소엽에서 세포내 대사율 저하를 암시하는 물질들의 변형과 핵 손실이 나타났으며 수컷보다는 암컷 흰쥐의 간세포 변화가 급격하였다. 5) Sudan Black B로 지질과 지방세포를 염색한 결과 실험군이 대조군에 비해 지방침착정도가 낮았고 암컷 흰쥐가 수컷보다 지방성분이 감소하였다. 따라서 비만치료제로 쓰이는 태음조위탕은 간기능에 부정적 영향을 주지않으며 간내 지방축적을 감소시키고 암컷 흰쥐에서 효과가 더 있는 것으로 나타났다. Yoon, J.H., Ryu, S.H., Jeong, K.H., Choi, D.G, Jeong, I.K., Lee, H.H, Kim, J.O., Lim, EM. Effects of 12 Weeks Taeyeumjoweetang Administration on Enzymes and Fat Accumulation in Rat Liver Cells. Exercise Science, 11(1): 53-65, 2002. Taeyeumjoweetang is associated with improving liver function as well as lowing increased levels of lipid profiles. These factors are known to reduce body fat mass in 'the Taeyeum person'. The purpose of this study was to investigate the effects of acute Taeyeumjoweetang administration on enzymes and fat accumulation in rat liver cells. Sixteen Sprague-Dawley rats(16weeks old) were randomly assigned into two groups; the control group(n=8) and the experimental group(n=8). The experimental rats were given the consumption of 50㎖ of the herb tea a day for 12 weeks. The data for TC, LDL, HDL and AI were measured in plasma blood, and GOT and GPT were measured in liver. An alpha level of .05 was used as the criteria for significance. The obtained results are as follows: 1. There were significant differences in body weight between the two groups. The herb-intake rats were lower than Placebo rats in body weight, but no significant differences in liver weight. 2. There were significant differences in TC, LDL and AI between the two groups. The herb-intake rats were lower than placebo rats in body weight. .But HDL levels in the herb-intake rats were highly than placebo rats. 3. There were no significant differences in GOT, GPT between two groups. But the levels in the herb-intake rats were highly than placebo rats. 4. It was implied that female rats may have more changes in liver cells and nucleus lose than male rats in the herb-intake group. 5. As results of lipid and adipose cell stain, the herb-intake rats were lower than placebo rats in fat accumulation, and female rats had lower fat than male rats in the herb-intake group. Therefore, the data suggest that Taeyeumjoweetang administration has an effect to reduce fat accumulation without the side effects especially in female rats.
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류호성,류성환,장민,구세광,권영삼,서민수 한국임상수의학회 2021 한국임상수의학회지 Vol.38 No.6
The study was conducted to evaluate the effects of carboxymethyl chitosan (CMC) on proliferation, migration, and lipopolysaccharide (LPS)-induced inflammatory response in canine bone marrow-derived mesenchymal stem cells (BMSCs). The proliferation and migration of BMSCs were examined after treatment with CMC. The effect of CMC on the mRNA expression of inflammatory cytokines, such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, IL-10, and transforming growth factor (TGF)-β, was also evaluated by reverse transcription polymerase chain reaction (RT-PCR). In the proliferation assay, no significant changes were found at all CMC concentrations compared with controls. The migration assay showed that CMC dose-dependently stimulated the migration of BMSCs in normal and LPS-treated conditions. RT-PCR showed that TNF-α and IL-10 expressions were suppressed in the BMSCs after CMC treatment. However, other genes were not affected. Taken together, CMC promoted BMSC migration and inhibited TNF-α and IL-10. Therefore, CMC may be possible to regulate wound healing when mesenchymal stem cells are applied in inflammatory diseases.
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