Immune modulation effect of probiotics in ageing mouse

( Sook In Ryu ),( Yi Haw Ji ),( Dae Won Park ),( Il-hwan Kim ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: With aging, immune senescence increases inflammation. The immune modulation effect of probiotics has been studied, but its mechanism needs to be elucidated. Objectives: To investigate the immune modulation effect of probiotics in aging mice. Methods: Six week-old mice(group A) and 14 month-old mice(group B & C) were each fed with saline(group A and B) and probiotics(group C). After 5 weeks, their spleens and blood were collected. Splenocyte suspensions were prepared and CD4+25- and CD4+25+ lymphocyte populations were analyzed with flow cytometry. The presence of INF-γ, IL-6 and TNF-α were measured in the splenic macrophage supernatants and blood serum samples before and after treatment with LPS. Results: We measured the differences of the level of INF-γ, IL-6 and TNF-α before and after LPS stimulation and the differences were compared among the 3 groups. IL-6 and TNF-α showed significant differences between group B and C. The level of INF-γ, IL-6 and TNF-α in blood serum and the FACS analysis of lymphocytes showed no difference among the 3 groups. Conclusion: The probiotics group showed less increase of TNF-α and IL-6 compared to that of the old mice control group. However, the probiotics group did not show elevation of CD4+25- cells compared to the control group. Also, INF-γ, IL-6 and TNF-α level in blood serum showed no significant differences between the control and probiotics group. We are planning further research to investigate the immune modulation effect of probiotics.

<i>In vivo</i> genotoxicity evaluation of lung cells from Fischer 344 rats following 28 days of inhalation exposure to MWCNTs, plus 28 days and 90 days post-exposure

Kim, Jin Sik,Sung, Jae Hyuck,Choi, Byung Gil,Ryu, Hyeon Yeol,Song, Kyung Seuk,Shin, Jae Hoon,Lee, Jong Seong,Hwang, Joo Hwan,Lee, Ji Hyun,Lee, Gun Ho,Jeon, Kisoo,Ahn, Kang Ho,Yu, Il Je Informa Healthcare 2014 INHALATION TOXICOLOGY Vol. No.

<P>Despite their useful physico-chemical properties, carbon nanotubes (CNTs) continue to cause concern over occupational and human health due to their structural similarity to asbestos. Thus, to evaluate the toxic and genotoxic effect of multi-wall carbon nanotubes (MWCNTs) on lung cells <I>in vivo</I>, eight-week-old rats were divided into four groups (each group = 25 animals), a fresh air control (0 mg/m<SUP>3</SUP>), low (0.17 mg/m<SUP>3</SUP>), middle (0.49 mg/m<SUP>3</SUP>), and high (0.96 mg/m<SUP>3</SUP>) dose group, and exposed to MWCNTs <I>via</I> nose-only inhalation 6 h per day, 5 days per week for 28 days. The count median length and geometric standard deviation for the MWCNTs determined by TEM were 330.18 and 1.72 nm, respectively, and the MWCNT diameters ranged from 10 to 15 nm. Lung cells were isolated from five male and five female rats in each group on day 0, day 28 (only from males) and day 90 following the 28-day exposure. The total number of animals used was 15 male and 10 female rats for each concentration group. To determine the genotoxicity of the MWCNTs, a single cell gel electrophoresis assay (Comet assay) was conducted on the rat lung cells. As a result of the exposure, the olive tail moments were found to be significantly higher (<I>p</I> < 0.05) in the male and female rats from all the exposed groups when compared with the fresh air control. In addition, the high-dose exposed male and middle and high-dose exposed female rats retained DNA damage, even 90 days post-exposure (<I>p</I> < 0.05). To investigate the mode of genotoxicity, the intracellular reactive oxygen species (ROS) levels and inflammatory cytokine levels (TNF-α, TGF- β, IL-1, IL-2, IL-4, IL-5, IL-10, IL-12 and IFN-γ) were also measured. For the male rats, the H<SUB>2</SUB>O<SUB>2</SUB> levels were significantly higher in the middle (0 days post-exposure) and high- (0 days and 28 days post-exposure) dose groups (<I>p</I> < 0.05). Conversely, the female rats showed no changes in the H<SUB>2</SUB>O<SUB>2</SUB> levels. The inflammatory cytokine levels in the bronchoalveolar lavage (BAL) fluid did not show any statistically significant difference. Interestingly, the short-length MWCNTs deposited in the lung cells were persistent at 90 days post-exposure. Thus, exposing lung cells to MWCNTs with a short tube length may induce genotoxicity.</P>

광주지역 황사기간중의 대기 건성침적량의 특성에 관한 연구

류일광,이치영,강영식,노기환,김관천,유경석,강동구 광주보건대학 2002 論文集 Vol.27 No.-

The deposition amounts of Ferric that are naturally originated elements from earth crust. The rate was 14,993㎎/㎡ day at the rang yellow sand season, which was 20 times higher than at the ordinary times. Artifical earth crust element Cr were lower than the amount yellow sand an increase. Naturally originated elements origination, movment channel and mutual be close the correlations of elements each other and were lower than in artificially originated elements. Deposition amount of chang were higher before yellow sand, lower before yellow sand of origination, time were inverse proportion. The average concentration of liquefactive metals high, compare heavy matal and numerous of deposition amount. A1so gain liquefactive matal were rescattering numerous on account of little mass deduction.

법랑종양 기저세포암 1예

유화정,이호섭,김애리,김일환 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.3

Basal cell carcinoma(BCC) exists in great variety. Adamantinoid BCC is one of the rare types of BCC. Histologically, the tumor masses are surrounded by a layer of cells in which the nuclei tend to palisade. Inside this layer, the tumor masses consist of cells with elongated nuclei and stellate cytoplasm stretched as thin, connection bridges across empty spaces, producing adamantinoid appearance. We report a case of adamantinoid BCC in a 49 year-old woman who had a single, asymptomatic, dusky erythematous to black colored nodule on her right jaw area. (Korean J Dermatol 2003;41(3) : 380∼382)

광주천 본천상의 N-hexane 추출물에 관한 조사 연구

류일광,이치영,강영식,노기환 광주보건대학 1984 論文集 Vol.9 No.-

Waste-water pollution of Kwangju river was examined for six months from May, 1983 to Oct, 1983. For checking the waste-water pollution, 5 sampling positions were selected from main river. The obtained results were as followings. 1) The concentration of N-hexane extraction matter in Kwangju river were increased gradually from 31.9ppm in July. 2) The average concentration of N-hexane extraction matter in mid-stream (sampling position in Kwangju bridge, Yangdong bridge and Kwangcheon bridge) was 12.4∼17.7ppm and in down stream(sampling position in Yuduck dong) was 23.6ppm. 3) In the relationship between N-hexane extraction matter and COD a strong and significant correlation coefficient(r=0.65) was calculated and the frequency distribute very closely along- the straight line equation, y=23.39x- 325.44. 4) From above facts the author found out that as the N-hexane extraction matter concentration in general river water coexist closely with other pollutant it is likely to be considered as new water pollution index.

榮山江의 水質汚染에 관한 연구

류일광,이치영,김병환,강영식 광주보건대학 1981 論文集 Vol.6 No.-

Water quality of Yeong San River was examined for 3 months from October 1. 1980 to December 1. 1980. For checking the water quality, 6 sampling positions were selected. The results we obtained are as follows. 1. The average for Yeong San River was 6.9∼7.6 of pH, 0.2∼7.3 ppm of Biochemical oxygen demand (BOD), 9∼236 ppm of Suspended solid (SS), 3.04∼1.59.26. ppm of Total Phosphate, 0.7∼24.62 ppm of Total. Nitrogen. 2. The phytoplankton identification in this survey period showed Cyanophyceae is 7 genera 9 species, Bacillariophyceae 14 genera 25 species and Chlorphyceae 21 genera 26 species: total 42 genera 60 species. 3. The results of biological water analysis were as follows: st. 1 was α msosaprobic and st.2 seemed to be oligosaprobic. St.3 was from α-mesosaprobicto β-mesosaprobic and st. 4 seemed to be β-mesosaprobic. St.5 was from β-mesosaprobic to oligosaprobic and st.6 seemed to be β-mesosaprobic. 4. To appear dominat algea there were Microcyctis, oscillatoria, Anabaena, Melosira, Nitzschia and Synedra.

김치 발효중 생체활성 아민 생성에 관한 연구

서일원,류춘선,이주돈,이영환,변광의 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

Biogenic amines can be formed and degraded either as the result of endogenous amino acid decarboxylase activity in raw materials or by the growth of decarboxylase-positive microorgannisms under conditions favorable to enzyme activity. Three biogenic amines(Tryptamine, Histamine, Tyramine) were intensively investigated by the HPLC during Kimchi fermentation at 5℃. The patterns of microfloral changes in lactic acid bacterial group, Leuconostoc, Streptococcus and Lactobacillus, were similar to the typical low-temperature fermentation. The population of microbial growth were high after 10 days and gradually decreased. The levels of Tyramine were lowered(10mg/kg) during this mid-periods(8∼16 days) but very high in initial-(15mg/kg) and final-periods (30mg/kg), in which the total acidity ranged between 0.2∼0.35% and 0.4∼0.6%, while the acidity in mid-periods revealed 0.35∼0.4% beginning the initial ripening period. Leuconostoc was highly increased in number in mid-period but slightly declined as the acidity increased, whereas Streptococcus was highly distributed in initial-period and Lactobacillus in final-period. The levels of Tryptamine and Histamine were relatively low throughout the whole fermentation period, but the profile of Tryptamine level was very similar to the case of Tyramine. Histamine was maintained at a certain level after mid-period of fermentation.

7個 結晶系의 唯一 X-線 回折强度 資料

徐日煥,金光柱,秋錦洪,李珍昊,林星秀,柳保盈,金文執 충남대학교 자연과학연구소 1992 忠南科學硏究誌 Vol.19 No.1

한 結晶의 構造는 唯一單位의 X-線 回折强度資料로부터 決定될 수 있다. Miller指數(hkl)의 函數인 回折强度Ⅰ(hkl)는 異常 散亂(anomalous scattering)이 無視될때 對稱中心點群 卽 Laue群의 對稱性을 나타낸다. 이 論文에서는 모든 結晶系에 對한 回折强度의 唯一單位를 Laue群들의 對稱性으로부터 誘導하여 놓았다. Crystal structures can be determined from the unique data of X-ray intensity. As the X-ray intensity Ⅰ(hkl), which is a function of diffraction plane (hkl), has a center of symmetry assuming anomalous scattering is negligible Ⅰ(hkl) shows the symmetry of a centrosymmetric point group, i.e., a Laue group. In this paper the unique data of the intensity for all crystal systems have been derived from the symmetries of Laue groups.

Reciprocal Lattice Explorer의 原理와 使用法

徐日煥,李珍昊,秋錦洪,林星秀,柳保盈,朴晶蘭,金文執,趙素羅,金憲俊,李正秀,成百石 충남대학교 자연과학연구소 1993 忠南科學硏究誌 Vol.20 No.2

Reciprocal lattice explorer는 de Jong-Bouman camera와 Buerger precession camera를 合친것이다. 이 explorer를 使用하면 goniometer에 한번 固定한 試料를 使用하여 그 試料의 空間群을 決定하는데 必要한 모든 寫眞을 撮影할 수 있으며 그들은 찌그러지지 않은 逆格子面의 寫眞들이다. 本 論文에서는 de Jong-Bouman 과 Buerger precession 寫眞術의 原理와 使用法을 記述 하였고 또한 de Jong-Bouman의 振動寫眞과 zeroth layer 寫眞으로부터 11個 Laue群의 識別方法도 提示하였다. The reciprocal lattice explorer is an instrument which incorporates the principles of both the Buerger precession and the de Jong-Bouman methods. On the explorer all photographs which are necessary for a space group determination can be taken with a single setting of a crystal and the photographs show undistorted pictures of the reciprocal lattice planes. In this paper, the principles and the usages of the de Jong-Bouman and the Buerger precession photography are described, and a method of 11 Laue group identification using the de Jong-Bouman oscillation and zeroth layer photographs is shown.

結晶學에 關한 論題(Ⅳ)

徐日煥,李珍昊,秋錦洪,李正秀,成百石,林星秀,柳保盈,朴晶蘭 충남대학교 자연과학연구소 1993 忠南科學硏究誌 Vol.20 No.1