셀레콕시브 및 그 합성유도체들의 항암활성 스크리닝

박정란,강진형,구효정,노지영,류형철,박상욱,고동현,조일환,이주영,황다니엘,김인경 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Selective COX (cyclooxygenase)-2 inhibitors including celecoxib have been shown to induce apoptosis and cell cycle changes in various tumor cells. New inhibitors are recently being developed as chemomodulating agents. We evaluated celecoxib and screened 150 synthetic compounds for anti-proliferative activities in vitro. Effects of celecoxib on COX activity, cell growth, cell cycle distribution, and apoptosis induction were determined in A549 COX-2 overexpressing human non-small cell lung cancer (NSCLC) cells. The COX inhibition of celecoxib increased with concentration up to 82% at 1μM after 24 hr exposure. Forty μM and 50μM of celecoxib induce G_1 arrest, and TUNEL-positive apoptotic cells, respectively. Among 150 compounds, several compounds were selected for having greater COX-2 inhibitory activity and higher selectivity than celecoxib with growth inhibitory activity. Celecoxib showed concentration-dependent COX inhibitory activity, and ability to induce cell cycle arrest and apoptosis in human NSCLC cells in vitro. Among synthetic analogues screened, several compounds showed promising in vitro activity as COX-2 inhibitory anticancer agents, which warrant further evaluation in vitro and in vivo.

검사정보시스템을 이용한 수혈 적정성 감시 전산 프로그램 개발

박정란,김신영,김진주,한양선,김효식,배인철,김현옥 대한수혈학회 2009 大韓輸血學會誌 Vol.20 No.3

Background: Careful consideration should be given administering a blood transfusion according to the transfusion criteria because blood components may cause various adverse reactions. In the future, a shortage of blood is inevitable due to strengthening the criteria of donor deferral and the increasing population of aged people, and this will cause a significant dearth of the blood supply. Therefore, we have developed a computerized blood auditing program for reducing the amount of blood transfused by changing the transfusion practices of clinicians. Methods: The blood audit program was developed to automatically check the clinical information, the pretransfusion laboratory test results, the operation etc of patients who are undergoing transfusion based on the laboratory information system (LIS). The criteria for appropriateness were based on the national transfusion guideline and the transfusion criteria of Severance Hospital. We evaluated the transfusion appropriateness of transfusing red blood cells (RBCs) and fresh frozen plasma (FFP) from April, 2009 to June, 2009 using this audit program. Results: RBCs were transfused to 2,353 patients over 5,652 episodes, and a total of 11,055 units were transfused. FFP was transfused to 574 patients over 1,228 episodes and a total of 4,258 units were transfused. We found that 1,120 (19.9%) RBC transfusion episodes and 377 (30.7%) FFP transfusion episodes were inappropriate. The proportion of inappropriate transfusion was higher in surgical departments than that in medical departments. Conclusion: Our computerized audit program evaluated a high number of transfusions in a short time, and we obtained results reflecting the entire past history of transfusions, and we can continuously audit transfusion using this program. We think that feedback to physicians who order transfusions would improve the appropriate use of transfusion. 배경: 혈액제제는 부작용이 다양하여 수혈은 그 적응증에 따라 신중히 결정할 필요가 있다. 또한 헌혈자 문진 기준의 강화와 인구의 고령화 등으로 인해 앞으로 혈액제제가 부족할 가능성은 계속 제기되고 있어 혈액제제를 적절하게 관리해야 할 필요성이 증대되고 있다. 따라서 환자에게 적절한 수혈을 시행하고, 혈액 사용을 감소시키기 위해 수혈 적정성 전산 감시 프로그램을 개발하였다. 방법: 수혈 적정성 감시 프로그램은 검사실정보시스템 자료를 기반으로 수혈 받은 환자의 진료정보와 수혈 전 혈액학적 검사 소견, 당일 수술여부 등을 자동으로 조회할 수 있도록 개발하였다. 평가 기준은 국가 수혈가이드라인과 세브란스병원의 수혈 기준을 기초로 하였다. 이 프로그램을 사용하여 2009년 4월에서 2009년 6월까지 시행된 적혈구제제와 신선동결혈장 수혈에 대하여 수혈 적정성을 평가하였다. 결과: 3개월 동안 적혈구제제 수혈은 총 2,353명에게 5,652회에 걸쳐 11,055단위가 수혈되었고, 신선동결혈장은 574명에게 1,228회 수혈되었고 총 4,258단위가 수혈되었다. 적혈구제제 부적절수혈은 1,120회(19.9%)였으며 신선동결혈장 부적절한 수혈은 377회(30.7%)였다. 부적절수혈 비율은 내과계보다 외과계에서 높았다. 결론: 전산 감시 프로그램은 짧은 시간 안에 많은 양의 수혈을 평가하여 이전의 전체적인 수혈 상황을 반영할 수 있는 결과를 얻었고, 앞으로도 지속적으로 감시활동을 할 수 있게 되었다. 우리는 이 평가 결과를 수혈을 처방한 주치의들에게 피드백하여 수혈적정성을 높일 수 있을 것으로 생각한다.

여성들의 직업병이 심각하다

박정란 세계평화교수협의회 1987 廣場 Vol.168 No.-

일본의 지역사회 중심 재해지원 사회복지실천과 시사점

박정란 한국가족사회복지학회 2014 가족정책포럼 Vol.3 No.-

지역사회복지 측면에서 본 일본 개호보험과 시사

박정란 한국지역사회복지학회 2008 한국지역사회복지학회 학술대회 Vol.2008 No.-

여성 새터민의 진로의사결정 연구

박정란 한국사회학회 2006 한국사회학회 사회학대회 논문집 Vol.2006 No.-

Salivette으로 채취한 타액의 아밀라제 측정

박정란,김미혜,우정민,이승재,송경은 대한진단검사의학회 2008 Annals of Laboratory Medicine Vol.28 No.6

Background : Saliva is increasingly being used as a specimen for systemic disease as well as for oral health status. Especially, salivary amylase has been studied as an excellent index for psychological stress. Authors evaluated the measurement of salivary amylase activities collected by Salivettes (Sarstedt, Germany). Methods : Saliva specimens were collected from 13 healthy adults between 10:00 and 11:00 a.m. Participants were asked to gently chew tampons of Salivettes for 1 min. Immediately after collection, all specimens were stored frozen. On the day of testing, they were centrifuged after thawing and diluted with distilled water. Amylase was measured by Dimension RxL Max (Dade Behring Inc.,USA). We evaluated precision, linearity, and recovery rate of Salivette. Amylase activities between collection of saliva by Salivette and passive drool were compared, and also the changes of amylase by the storage temperature were evaluated. Results : Intra-run CVs for three levels of amylase were excellent. Between-day CVs and total CVs were good only for mid and high levels. A good linear relationship was found at all diluted levels. Dosing Salivettes with 2 mL, 1.5 mL, and 1 mL yielded sample recovery 85.5±2.4%, 82.4±1.5%, and 72.2±3.1%, respectively and amylase recovery 78.9±10.9%, 74.1±13.7%, and 37.3±26.9%, respectively. Amylase by Salivette and passive drool were correlated well (r=0.757), although they showed a significant difference. Amylase activity was not affected by the storage temperature. Conclusions : Measurement of salivary amylase using Salivette could be a useful test having good intra-run CVs and linearity. More than 1.5 mL of saliva would be needed to have more than 70% recovery of Salivette. (Korean J Lab Med 2008;28:438-43)

치위생학 전공 학생들의 소명의식 및 자아존중감에 따른 전문직업성 정도 연구

박정란,김해선 기독보건학회 2012 전인건강과학회지 Vol.2 No.1

공사립 유치원 에듀케어 프로그램의 혼합연령학급 운영 실제 및 교육적 효과에 대한 교사 인식

박정란 한국영유아교원교육학회 2007 幼兒 敎育學論集 Vol.11 No.2

The Public and Private Kindergarten Teachers' Recognition of Edu-care Program Practice and Educational Effects Regarding the Mixed-aged Group

Stat1/IRF1 can modulate alveolar inflammatory response in lipopolysaccharide-induced acute lung injury

박정란,이한별,이주연,장수진,양세란 대한결핵 및 호흡기학회 2015 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.120 No.-

Acute lung injury (ALI) or its severe form, acute respiratory distress syndrome (ARDS), contributes to be important cause of morbidity and mortality due to a lack of specific, effective therapy. The mechanisms of ALI/ARDS are largely unknown. In the present study, mice were challenged with intratracheal instillation of LPS to induced ALI. In response to LPS challenge, neutrophils were recruited and myeloperoxidase activity, nitric oxide levels and inflammatory cytokines were significantly increased in the lung and bronchoalveolar lavage fluid of mice. We performed the next generation sequencing technology for the new molecular approaches of ALI/ARDS. In NGS data, 661 genes were significantly up-regulated while 515 genes were down-regulated with 2-fold change in the lung of LPS challenge compared with controls. Here, we found that interferon regulatory factor 1(IRF1) was significantly increased in the lung tissue, LPS-treated mouse peritoneal macrophages and neutrophils. Moreover, IRF1 mediates gene induction downstream of signal transducer and activator of transcription 1 (Stat1). We determined that the levels of pStat1 in LPS challenge were higher than in controls. The conclusion of this study is that Stat1/IRF1 function as components of the signaling pathway that mediates LPS-induced ALI/ARDS. Taken together, our data suggest that Stat1/IRF1 may potentially be considered as a new molecular target of an ALI/ARDS treatment. This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2014R1A2A2A01003737).