중합효소연쇄반응을 이용한 ACTPB2 유전자와 ApoB3'유전자의 유전적 다형성에 관한 연구 : 친자감별검사를 위한 유용성 평가 Evaluation to the Paternity Testing Among Koreans

박영석,이경옥,박택규,오문주,김은하,김윤정 한국유전학회 1995 Genes & Genomics Vol.17 No.4

The class of polymorphic loci, known as VNTR (variation number of tandem repeat), are highly polymorphic in humans. In the present study, allele and genotype frequencies of the Human β-actin related pseudogene (ACTPB2) and Apolipoprotein B (ApoB) gene were determined among Koreans by using amplified fragment length polymorphism (Amp-FLP) technique. In ACTPB2 gene, 23 alleles and 36 genotypes were observed, whereas 11 alleles and 22 genotypes were determined in ApoB 3' gene among over 100 unrelated Koreans. In ACTPB2 and ApoB 3' genes, heterozygosity (H) was 0.817 and 0.706, and the power of exclusion (POE) was 0.956 and 0.850, respectively. The probability of sharing the same DNA band was 2.75×10^(-2) for ACTPB2 and 14.7×10^(-2) for ApoB 3'. Mendelian inheritance for the alleles at the two VNTR markers could be demonstrated in 20 paternity cases. The mutation rate of ACTPB2 gene was 0.04, however, the mutated band was not found in ApoB 3' gene. The data presented here on allele frequencies and mutation rates provide preliminary data supporting the validity of ACTPB2 and ApoB 3' genes in paternity analysis among Koreans.

Electrochemical Properties of HNO3 Pre-treated TiO2 Photoanode Based Dye-sensitized Solar Cell

( Kyung Hee Park ),( En Mei Kim ),( Hal Bon Gu ) 조선대학교 공학기술연구원 2008 공학기술논문지 Vol.1 No.2

TiO2 photoanode was prepared using nitric acid pre-treated TiO2 materials. We compare dispersion of TiO2 nanoparticles before and after nitric acid treatment and measured Ti oxidized state from XPS. Low charge transfer resistance was obtained in nitric acid treated sample than that of untreated sample. The dye-sensitized solar cell based on the nitric acid treatment had open-circuit voltage of 0.71 V, a short-circuit current of 15.2 mAcm(-2) and an energy conversion efficiency of 6.6% under light intensity of 100 mWcm(-2). About 14% increases in efficiency obtained when the TiO2 electrode was treated by nitric acid.

Dye-sensitized solar cells based on electrospun poly(vinylidenefluoride-co-hexafluoropropylene) nanofibers

Park, Ju-Young,Lee, Jae-Wook,Park, Kyung Hee,Kim, Tae-Young,Yim, Soon-Ho,Zhao, Xing Guan,Gu, Hal-Bon,Jin, En Mei Springer-Verlag 2013 Polymer bulletin Vol.70 No.2

Thermal Tuning System for PEFP DTL Resonant Frequency Control

Kyung Ryul Kim,Jun Park,Hyung Gyun Kim,Hee Seob Kim,Woon Ha Hwang,Chong Chul Yoon,En Byul Cho,Hyeok Jung Kwon,Han Sung Kim,Yong Sub Cho 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.5

The Proton Engineering Frontier Project (PEFP) has been under development to establish a 100 MeV proton accelerator complex in Korea. As a front part of the 100-MeV machine, 20-MeV proton accelerator systems were installed and tested at the site of the Korea Atomic Energy Research Institute (KEARI). The PEFP accelerator structures, such as the drift tube linac (DTL), require a water-cooling-system-based thermal tuning system to regulate the resonant frequency (rf) of the drift tube (DT) cavity to 350 MHz. We have designed and fabricated a prototype water pumping skid that has two water cooling channels of a by-passing cooling water (primary loop) and a plate heat exchanger (secondary loop). The required operating temperature can be achieved by mixing the coolants of each channel. The temperature control around the operating point could be achieved using two independent PI controllers installed in the two loops. The system set-up and test were conducted for the 20 MeV DTL systems. The results indicate that the temperature control of the drift tube cavities is available within design range of 27±6℃ with a precision of less than ±0.1℃ to regulate the resonant frequency of the drift tube cavities. The Proton Engineering Frontier Project (PEFP) has been under development to establish a 100 MeV proton accelerator complex in Korea. As a front part of the 100-MeV machine, 20-MeV proton accelerator systems were installed and tested at the site of the Korea Atomic Energy Research Institute (KEARI). The PEFP accelerator structures, such as the drift tube linac (DTL), require a water-cooling-system-based thermal tuning system to regulate the resonant frequency (rf) of the drift tube (DT) cavity to 350 MHz. We have designed and fabricated a prototype water pumping skid that has two water cooling channels of a by-passing cooling water (primary loop) and a plate heat exchanger (secondary loop). The required operating temperature can be achieved by mixing the coolants of each channel. The temperature control around the operating point could be achieved using two independent PI controllers installed in the two loops. The system set-up and test were conducted for the 20 MeV DTL systems. The results indicate that the temperature control of the drift tube cavities is available within design range of 27±6℃ with a precision of less than ±0.1℃ to regulate the resonant frequency of the drift tube cavities.

Characterization of Mesoporous TiO2 Nanocrystalline Films for High Performance of Dye-Sensitized Solar Cells

( Kyung Hee Park ),( En Mei Kim ),( Hal Bon Gu ) 조선대학교 공학기술연구원 2008 공학기술논문지 Vol.1 No.1

Titanium dioxide (TiO2) thin films were deposited by sol-gel method with surfactant-assisted mechanism and its application for dye-sensitized solar cells (DSSCs) was investigated. Brunauer-Emmertt-Teller (BET), X-ray diffraction (XRD) and field emission scanning electron microscopy (FE-SEM) techniques were used to characterize the surface characteristics of thin films. Photovoltaic-current density measurements were carried out to determine the photoelectrochemical properties of the thin films and the performance of DSSCs. Energy conversion efficiency of about 6.1% has been achieved for cell with conductive glass under illumination with AM 1.5 (100 mWcm(-2)) simulated sunlight. Investigations had showed higher photo-energy conversion efficiency for mesoporous TiO2 nanocrystalline films used in DSSCs relatively to commercially available Degussa P25 films.

반복적인 관절염으로 발현된 베체트병 1례

박석우,이승민,오지은,김자경,김정희,임대현,손병관,송정수 대한 소아알레르기 및 호흡기학회 2004 소아알레르기 및 호흡기학회지 Vol.14 No.2

女性公務員에 대한 實態調査

박순영,오용,유은주,유재경,정남옥 이화여자대학교 법정대학 정치외교학과 1975 정치외교연구 Vol.3 No.-

1. 여성공무원은 20∼25세의 젊은 층이 많았다. 2. 여성공무원은 미혼의 여성이 대부분이며 미혼일 경우는 일반적으로 부친의 직업이 불안정하며 기혼여성의 남편은 대부분이 공무원, 회사원이었다. 3. 여성공무원들은 대부분 고졸이상이며 대학졸업이상은 고졸이상의 반수를 넘지 못하고 있었다. 4. 직급은 5급 을류가 대부분으로 여성공무원은 대부분 하급공무원이며 고급공무원(3급 이상)은 5명에 불과 하였다. 5. 채용에 있어서는 추천이 대부분이고 추천의 경우는 대졸이상인 경우가 고졸이상보다 더 많다. 공개시험으로 채용된 사람은 정규직이 대부분이나 서류전형, 추천은 고용직, 임시직이 대부분을 차지하고 있었다. 6. 채용시 특혜를 바라는 공무원은 공개시험으로 채용된 여성은 필요없다고 하고 있었다. 사류전형에 의한 여성공무원이며, 특혜를 원하고 있었다. 특혜는 시험성적보다는 여성끼리의 경쟁으로 하기를 바라고 있었다. 7. 직무내용에 있어서는 행정적 업무가 반수를 차지하고 대부분이 기술적 업무나 비서직에 머물고 있다. 8. 근속연한은 3년미만이 대부분이다. 9. 여성공무원들은 대부분 자기의 부처에 대해 관심을 갖고 있으나 근무처에 대부분의 여성공무원이 불만을 가지고 있다. 특히 대학졸업 이상인 경우가 더 많은 불만을 가지고 있다. 10. 근무처에 만족하지 않는 큰 이유는 보수와 승진의 제한에 있으며 업무의 불만은 상관에게 직접 호소하고 있다. 그러나 아직도 많은 여성공무원들은 그들의 불만을 해소하기 보다는 단념한다. 11. 자기가 맡은 업무에 대한 열성도는 보통이다. 12. 현 여성공무원들은 여성에게 적합한 직무가 창의적인 능력을 요구하는 업무나 감독업무보다는 단순 반복적인 업무라고 생각하고 있다. 13. 여성과 남성을 비교하여 업무수행의 장, 단점에서 장점은 여성이 남성보다 성실하고 세심하게 일하며 분위기 조성을 잘한다고 생각하며 단점은 야성이 남성과 차별대우를 받는다고 생각한다. 14. 업무수행에서 대졸이상일 경우는 전문적 지식을 요구하고 있고 공개채용된 사람보다 서류전형으로 채용된 사람이 기술이나 전문적 지식이 필요하다고 보고 있었다. 15. 업무수행상 법률적 지식이 필요한 경우는 꼭 필요한 경우보자는 신속성이 있는 것이 더 좋다고 하고 있었다. 16. 전문적 업무에 종사하는 공무원이 기술적 업무나 행정적 업무에 종사하는 공무원보다 법률적 지식을 더 필요로 하고 있었다. 17. 고급관리가 하급관리들보다 법률적 지식을 더 필요로 하고 있었다. 18. 업무수행상 불쾌한 일이 생기는 경우는 대인관계에서 생기는 경우로 가장 높은 비율을 보이고 있다. 19. 여성공무원들의 신분보장은 조금씩은 되고 있으나 확실히 되고 있다고 자신하는 사람은 극히 마약하다. 20. 신분보장이 안되는 이유를 여성공무원들은 대부분이 충분한 보수를 받고 있지 않다고 들고 있다. 21. 직무수행상 여성공무원들이 느끼는 애로점은 가정 사정이나 능력 부족 보다는 남녀차별에 의한 인사관리상의 문제였다. 22. 여성공무원이 장기근무하지 못하는 이유는 결혼 및 가정생활이 단연 우세하다. 23. 직무수행에 있어 협력이 잘되는 경우는 여성끼리보다는 여성과 남성이 함께 일 할 때였다. 그들은 여성공무원들이 될 사람들에게 책임, 성실, 협동, 인내, 참여의식을 갖기를 바라고 있었다. 24. 그들은 교육기관에 교양, 상식 및 법률, 실시(타자), 어학(한문)등을 여성공무원이 될 사람들에게 가르치기를 원하고 있었다. 25. 여성공무원들은 직무수행에 대한 포상보다는 상관이나 동료직원들이 그들의 직무수행을 인정할 때 더 열심히 일하고 싶어한다. 26. 여성의 대우상에 대한 난점은 여성에 대한 편견이 절대적이며 승진과 보직의 차별도 중시하고 있다. 27. 추천, 서류전형으로 채용된 여성공무원이 공개시험으로 채용된 여성 공무원보다 여성에 대한 편견을 난점으로 들고 있었다. 28. 남성공무원과 동등하게 능력개발을 위한 훈련을 시키지 않고 있다고 하고 있었다. 29. 인사행정이 비교적 공정하다고 하고 있으나 그들은 대부분 불공정하거나 또는 모른다고 하고 있었다. 30. 그들은 근무성적을 책임감과 적극성으로 평가해주기를 원한다. 31. 여성공무원들은 각 부처에서 그들 자신을 대부분 대체로 좋게 평가하여 주거나 보통 알아준다고 말하고 있었다. 32. 여성공무원의 수가 적당하거나 너무 적다고 말한 사람이 대부분으로 아직도 여성 공무원의 수는 부족하다. 33. 그들은 공무원이 되면서 국민에게 봉사하는 것보다는 보수로 휴가를 얻어 생활을 즐기거나 전공지식 활용을 하기를 원하고 있었다. 34. 그들이 가장 가고 싶은 행정부처는 외무부, 문교부, 재무부, 총무부, 보사부, 상공부의 순으로 나타났다. 35. 그들은 공무원 생활에 때때로 보람을 느끼고 있었다. 36. 그들은 여성공무원에게 적합한 퇴직연령이 30세 미만이라고 하고 있으며 적합한 최고 직책은 국장이나 장·차관이상이라고 하고 있었다. 37. 그들은 여성공무원의 지위향상을 위한 독립된 조직이 절대로 필요하다고 하고 있었다.

Electrochemical Characteristics of LiFePO4 with Conductivity Materials for Lithium Polymer Batteries

( En Mei Kim ),( Kyung Hee Park ),( Hal Bod Gu ) 조선대학교 공학기술연구원 2008 공학기술논문지 Vol.1 No.1

Phospho-olivine LiFePO4 cathode materials were prepared by hydrothermal reaction. In this study, Multi-walled carbon nanotube (MWCNT) and Carbon black was added to enhance the electrical conductivity of LiFePO4. LiFePO4, LiFePO4-MWCNT and LiFePO4-C particles were characterized by X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM) transmission electron microscope (TEM). LiFePO4/SPF/Li, LiFePO4-MWCMT/SPF/Li and LiFePO4-C/SPE/Li cells were characterized electrochemically by charge/discharge experiments at a constant current density of 0.1 mA cm(-2) in a range between 2.5 and 4.3 V vs. Li/Li(+) and cyclic voltammetry (CV). The results showed that initial discharge capacity of LiFePO4 was 103 mA h g(-1). The discharge capacity of LiFePO4-MWCNT/SPF/Li cell with 5 wt % MWCNT was 124 mAh g(-), LiFePO4-C/SPE/Li cell with 5 wt % carbon black was 128 mAh g(-1) at the first cycle.

Induction of Apoptotic Cell Death in Human Jurkat T Cells by a Chlorophyll Derivative(Cp-D)Isolated from Actinidia arguta Planchon

Park, Youn Hee,Chun, En Mi,Bae, Myung Ae,Sue, Young Bae,Song, Kyung Sik,Kim, Young Ho 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.1

The chloroform and methanol (2:1, v/v) extract from an edible plant, Actinidia arguta Planchon, appeared to possess antitumor activity against human leukemias Jurkat T and U937 cells through inducing apoptosis. The substance in the solvent extract was purified by silica gel column chromatography, preparative TLC, and Sephadex LH-20 column chromatography. Characteristics of the substance analyzed by UV scanning analysis, and ^1H and ^13C NMR spectra suggested that the substance belongs to the chlorophyll derivatives-like group. The IC_50 value of the chlorophyll derivative (Cp-D) determined by MTT assay was 15㎍/㎖ for Jurkat, 10㎍/㎖ for U937, and 11.4㎍/㎖ for HL-60, and was more toxic to these leukemias than to solid tumors or normal fibroblast. In order to elucidate cellular mechanisms underlying the cytotoxicity, the effect of the Cp-D on Jurkat T cells was investigated. When cells were treated with the Cp-D at a concentration of 15㎍/㎖, [^3H]thymidine incorporation declined rapidly and was undetectable in 1 h. However, no significant changes were made in the cell cycle distribution of the cells by 24h. The sub-G1 peak representing apoptotic cells began to be detectable in 36h, at which time apoptotic DNA fragmentation was also detected on agarose gel electrophoresis, demonstrating that the cytotoxic effect of the Cp-D is attributable to the induced apoptosis. Under the same conditions, although the protein level of cyclin-dependent kinases such as cdc4, cdk6, cdk2, and cdc2 was not significantly changed until 24h, the kinase activity of all cdks rapidly declined and reached a minimum level within 1-6h and then recovered to the initial level by 12h and sustained until 24h. These results suggest that inactivation of cdks at an inappropriate time during the cell cycle progression in Jurkat T cells following a treatment with the Cp-D leads to induction of apoptotic cell death.

The National Status of Tuberculosis Using Nationwide Medical Records Survey of Patients with Tuberculosis in Korea

Park, Yoon-Sung,Hong, Sung-Jin,Boo, Yoo-Kyung,Hwang, Eun-Suk,Kim, Hyun Jung,Cho, Seung-Hee,Na, Kyung-In,Cho, En-Hi,Shin, Sang-Sook The Korean Academy of Tuberculosis and Respiratory 2012 Tuberculosis and Respiratory Diseases Vol.73 No.1

Background: The epidemiology of tuberculosis (TB) has been assessed based on the data of the analysis of TB patients notified to the surveillance system in Korea. However, the national status of TB is not validated through this surveillance system. The objective is to determine the epidemiology of TB and to understand the accurate status of TB patients treated in private institutions. Methods: Medical records of 53,579 patients who had been diagnosed with TB in 2008 were analyzed. Results: Among 53,579 patients, the number of sputum smear positive cases was 15,639(29.2%) and the number of new cases was 39,191 (73.1%). The drug resistance rate of new cases was 5.3%, while the rate stood at 13.3% for TB patients with treatment history. The number of multi-drug resistant TB (MDR-TB) patients was 2,472 (4.6%), which consists of 2.9% of new cases and 9.3% of TB patients with prior treatment history. The number of extensively drug-resistant TB patients was 749 (1.4%), consisting of 1.1% of new cases and 2.2% of TB patients with prior treatment history. In terms of treatment outcomes, 66.4% of all TB patients, 70.5% of new cases, 64.4% of relapse cases, and 46.8% of MDR-TB cases were cured or completed. It was inferred that in 2008, the total number of TB patients reached 70,767, 145.6 per 100,000 people (95% confidence interval, 145.5~145.7). Conclusion: We conclude that the medical records review of the Health Insurance Review and Assessment Service (HIRA) data can be very effective in promoting the understanding of the current status of TB in private institutions.