Glucose regulated protein 78 promotes cell invasion via regulation of uPA production and secretion in colon cancer cells

( Zong Wei Li ),( Lic Hao Zhang ),( Han Qing Li ),( Shu Hua Shan ),( Zhuo Yu Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.8

Glucose regulated protein 78 (GRP78) is frequently highlyexpressed in tumor cells, contributing to the acquisition of severalphenotypic cancer hallmarks. GRP78 expression is also positivelycorrelated with tumor metastasis, and promotes hepatocellularcarcinoma cell invasion via increasing cell motility, however, other mechanisms involving the prometastatic roles of GRP78remain to be elucidated. Here we report that forced GRP78expression promotes colon cancer cell migration and invasionthrough upregulating MMP-2, MMP-9 and especially uPAproduction. These effects of GRP78 are mediated by enhancingthe activation of β-catenin signaling. Interestingly, we identify thatGRP78 interacts with uPA both in the cells and in the culturemedium, suggesting that GRP78 protein is likely to directlyfacilitate uPA secretion via protein-protein interaction. Takentogether, our findings demonstrate for the first time that besidesstimulation of cell motility, GRP78 can act by increasingproteases production to promote tumor cell invasion.

Study of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects

Wei Liu,Sheng-Ning Zhang,Zong-Qiang Hu,Shi-Ming Feng,Zhen-Hui Li,Shu-Feng Xiao,Hong-Shu Wang,Li Li 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.6

BACKGROUND: With the popularity of laparoscopic cholecystectomy, common bile duct injury has been reported more frequently. There is no perfect method for repairing porcine biliary segmental defects. METHODS: After the decellularization of human arterial blood vessels, the cells were cultured with GFP? (carry green fluorescent protein) porcine bile duct epithelial cells. The growth and proliferation of porcine bile duct epithelial cells on the human acellular arterial matrix (HAAM) were observed by hematoxylin–eosin (HE) staining, electron microscopy, and immunofluorescence. Then, the recellularized human acellular arterial matrix (RHAAM) was used to repair biliary segmental defects in the pig. The feasibility of it was detected by magnetic resonance cholangiopancreatography, liver function and blood routine changes, HE staining, immunofluorescence, real-time quantitative PCR (RT-qPCR), and western blot. RESULTS: After 4 weeks (w) of co-culture of HAAM and GFP? porcine bile duct epithelial cells, GFP? porcine bile duct epithelial cells grew stably, proliferated, and fused on HAAM. Bile was successfully drained into the duodenum without bile leakage or biliary obstruction. Immunofluorescence detection showed that GFP-positive bile duct cells could still be detected after GFP-containing bile duct cells were implanted into the acellular arterial matrix for 8 w. The implanted bile duct cells can successfully resist bile invasion and protect the acellular arterial matrix until the newborn bile duct is formed. CONCLUSION: The RHAAM can be used to repair biliary segmental defects in pigs, which provides a new idea for the clinical treatment of common bile duct injury.

Convenient Synthesis of N-Methylpyrrolidine-2-thione and Some Thioamides

Zong, Zhi-Min,Peng, Yao-Li,Liu, Zhi-Gang,Zhou, Shi-Lu,Wu, Lin,Wang, Xiao-Hua,Wei, Xian-Yong,Lee, Chul Wee 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.2

The synthesis of thioamides and thiolactams, which are used as important organic intermediates, has attracted great attention. However, expensive reagents, severe reaction conditions and low yields of the target products made conventional methods inconvenient and economically infeasible. To overcome these disadvantages, we investigated a new process for synthesizing thioamides and thiolactams. We examine thermal reactions of CS_2 with N-methyl-2-pyrrolidinones, formylamide, acetamide and N, N-dimethylformylamide, respectively. The results show that under optimum conditions N-methylpyttolidine-2-thione and the corresponding thioamides can be obtained in good to excellent yields by the above thionation reactions.

A multipath peroxymonosulfate activation process over supported by magnetic CuO-Fe3O4 nanoparticles for efficient degradation of 4-chlorophenol

Wei Peng,Jie Liu,Wei Peng,Chenxu Li,Fuxing Zong,Wensi Xu,Xing Zhang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.8

Heterogeneous catalysts with low cost, environmentally friendly, highly effective and ready separation from aqueous solution are highly desirable. Magnetic CuO-Fe3O4 nanoparticles, a type of non-toxic bimetallic transition metal oxide, is a promising heterogeneous catalyst for activation of peroxymonosulfate (PMS) to generate reactive oxygen species (ROS) that has not been previously investigated. In this study, the activation of PMS by CuO-Fe3O4 nanoparticles was evaluated using the degradation of 4-chlorophenol as a model reaction. Several critical factors such as pH, catalyst dosage and PMS concentration were investigated. CuO-Fe3O4/PMS system demonstrated a wide effective pH range to degrade 4-chlorophenol, namely 5.5 to 9.5. With the increase of the catalyst dosage, the degradation efficiency of 4-chlorophenol appeared to increase first and then decrease, that the inflection point was 0.5 g/L. Elevated PMS concentration obviously improved the decomposition of 4-chlorophenol; however, the plateau was reached when the PMS concentration was 8mM. Further increase in PMS concentration would not significantly improve the removal efficiency. Through examining the effects of scavengers and electron spin resonance (ESR) analyses, CuO-Fe3O4 nanoparticles were proven to activate PMS through a non-radical and radical pathway to generate singlet oxygen, sulfate radicals and hydroxyl radicals. Based on results, CuO-Fe3O4 nanoparticles were effective, environmentally friendly and low cost catalysts for efficient activation of PMS. These features make CuO-Fe3O4 nanoparticles a readily available heterogeneous catalyst to activate PMS for refractory organic pollutants degradation in advanced oxidation processes (AOPs).

Analysis of the Relationship between MHC-DRB1 Gene Polymorphism and Hydatidosis in Kazakh Sheep

Li, Ren-Yan,Jia, Bin,Zhang, Wen-Ju,Zhao, Zong-Sheng,Shi, Guo-Qing,Shen, Hong,Peng, Qiang,Lv, Li-Min,Zhou, Qi-Wei,Du, Ying-Chun Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.9

The objective of this work was to analyze the relationship between ovine major histocompatibility complex (MHC) DRB1 gene polymorphism and genetic resistance to hydatidosis in Kazakh sheep. The Ovar (ovine MHC) class II DRB1 second exon was amplified by polymerase chain reaction (PCR) from DNA samples of 702 Kazakh sheep, including 302 sheep with hydatidosis and 400 health controls. PCR products were characterized by the restriction fragment length polymorphism (RFLP) technique using five restriction enzymes, i.e., MvaI, HaeIII, SacI, SacII and Hin1I, yielding 14 alleles and 28 genotypes. Comparing the frequency of genotypes in hydatidosis sheep with the control group, it was found that the genotype frequencies of MvaIbc, Hin1Iab, SacIIab, HaeIIIde, HaeIIIdf and HaeIIIdd in control sheep were significantly (p<0.01) higher than in hydatidosis sheep, indicating that a significant correlation existed between these genotypes and resistance to hydatidosis. Genotype frequencies of MvaIbb, SacIIaa, Hin1Ibb and HaeIIIef in sheep with hydatidosis were extremely significantly (p<0.01) higher than in the control group, and the genotype frequency of HaeIIIab was significantly higher (p<0.05), indicating that a marked correlation existed between these genotypes and susceptibility to hydatidosis. By way of analyzing haplotype with these resistant genotypes, the hydatidosis resistant haplotype MvaIbc-SacIIab-Hin1Iab of Kazakh sheep was screened out, and then verified through artificial hydatid infection in sheep. The results indicated that the infection rate of sheep with the resistant haplotype of hydatidosis was significantly lower (p<0.01) than without this resistant haplotype. It showed that the genic haplotype MvaIbc-SacIIab-Hin1Iab of Ovar-DRB1 exon 2 was the resistant haplotype of hydatidosis in Kazakh sheep.

Efficacy and Safety of Adalimumab in Moderately to Severely Active Cases of Ulcerative Colitis: A Meta-Analysis of Published Placebo-Controlled Trials

( Zong Mei Zhang ),( Wei Li ),( Xue Liang Jiang ) 대한소화기학회 2016 Gut and Liver Vol.10 No.2

Background/Aims: To evaluate the efficacy and safety ofadalimumab (ADA) in moderately to severely active ulcerativecolitis (UC) patients who are unresponsive to traditional therapy. Methods: Electronic databases, including the PubMed, Embase, and Cochrane databases, were searched to April 20, 2014. UC-related randomized controlled trials (RCTs) that compared ADA with placebo were eligible. Review Manager 5.1 was used for data analysis. Results: This meta-analysis included three RCTs. ADA was considerably more effective compared with a placebo, and it increased the ratio of patients with clinical remission, clinical responses, mucosal healing and inflammatory bowel disease questionnaire responses in the induction and maintenance phases (p<0.05), as well as patients with steroid-free remission (p<0.05) during the maintenance phase. Clinical remission was achieved in a greater number of UC cases in the ADA 160/80/40 mg groups (0/2/4 week, every other week) compared with the placebo group at week 8 (p=0.006) and week 52 (p=0.0002), whereas the week 8 clinical remission rate was equivalent between the ADA 80/40 mg groups and the placebo group. Among the patients who received immunomodulators (IMM) at baseline, ADA was superior to the placebo in terms of inducing clinical remission (p=0.01). Between-group differences were not observed in terms of serious adverse events (p=0.61). Conclusions: ADA, particularly at doses of 160/80/40 mg (0/2/4 week, every other week), is effective and safe in patients with moderate-to-severe UC who are unresponsive to traditional treatment. Concomitant IMM therapy may improve the short-term therapeutic efficacy of ADA. (Gut Liver 2016;10:262-274)

PU.1 Is Identified as a Novel Metastasis Suppressor in Hepatocellular Carcinoma Regulating the miR-615-5p/IGF2 Axis

Song, Li-Jie,Zhang, Wei-Jie,Chang, Zhi-Wei,Pan, Yan-Feng,Zong, Hong,Fan, Qing-Xia,Wang, Liu-Xing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Invasion and metastasis is the major cause of tumor recurrence, difficulty for cure and low survival rate. Excavating key transcription factors, which can regulate tumor invasion and metastasis, are crucial to the development of therapeutic strategies for cancers. PU.1 is a master hematopoietic transcription factor and a vital regulator in life. Here, we report that, compared to adjacent non-cancerous tissues, expression of PU.1 mRNA in metastatic hepatocellular carcinoma (HCC), but not primary HCC, was significantly down-regulated. In addition, levels of PU.1 mRNA in metastatic hepatoma cell lines MHCC97L and MHCC97H were much lower than in non-metastatic Hep3B cells. Transwell invasion assays after PU.1 siRNA transfection showed that the invasion of hepatoma cell lines was increased markedly by PU.1 knockdown. Oppositely, overexpression of PU.1 suppressed the invasion of these cells. However, knockdown and overexpression of PU.1 did not influence proliferation. Finally, we tried to explore the potential mechanism of PU.1 suppressing hepatoma cell invasion. ChIP-qPCR analysis showed that PU.1 exhibited a high binding capacity with miR-615-5p promoter sequence. Overexpression of PU.1 caused a dramatic increase of pri-, pre- and mature miR-615-5p, as well as a marked decrease of miR-615-5p target gene IGF2. These data indicate that PU.1 inhibits invasion of human HCC through promoting miR-615-5p and suppressing IGF2. These findings improve our understanding of PU.1 regulatory roles and provided a potential target for metastatic HCC diagnosis and therapy.

Evaluation of Biogas Production Performance and Dynamics of the Microbial Community in Different Straws

( Xue Li ),( Yan-hua Liu ),( Xin Zhang ),( Chang-ming Ge ),( Ren-zhe Piao ),( Wei-dong Wang ),( Zong-jun Cui ),( Hong-yan Zhao ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3

The development and utilization of crop straw biogas resources can effectively alleviate the shortage of energy, environmental pollution, and other issues. This study performed a continuous batch test at 35°C to assess the methane production potential and volatile organic acid contents using the modified Gompertz equation. Illumina MiSeq platform sequencing, which is a sequencing method based on sequencing-by-synthesis, was used to compare the archaeal community diversity, and denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community diversity in rice straw, dry maize straw, silage maize straw, and tobacco straw. The results showed that cumulative gas production values for silage maize straw, rice straw, dry maize straw, and tobacco straw were 4,870, 4,032.5, 3,907.5, and 3,628.3 ml/g ·VS , respectively, after 24 days. Maximum daily gas production values of silage maize straw and rice straw were 1,025 and 904.17 ml/g ·VS, respectively, followed by tobacco straw and dry maize straw. The methane content of all four kinds of straws was > 60%, particularly that of silage maize straw, which peaked at 67.3%. Biogas production from the four kinds of straw was in the order silage maize straw > rice straw > dry maize straw > tobacco straw, and the values were 1,166.7, 1,048.4, 890, and 637.4 ml/g ·VS, respectively. The microbial community analysis showed that metabolism was mainly carried out by acetateutilizing methanogens, and that Methanosarcina was the dominant archaeal genus in the four kinds of straw, and the DGGE bands belonged to the phyla Firmicutes, Bacteroidetes, and Chloroflexi. Silage maize is useful for biogas production because it contains four kinds of straw.

Exploration of Molecular Mechanisms of Diffuse Large B-cell Lymphoma Development Using a Microarray

Zhang, Zong-Xin,Shen, Cui-Fen,Zou, Wei-Hua,Shou, Li-Hong,Zhang, Hui-Ying,Jin, Wen-Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Objective: We aimed to identify key genes, pathways and function modules in the development of diffuse large B-cell lymphoma (DLBCL) with microarray data and interaction network analysis. Methods: Microarray data sets for 7 DLBCL samples and 7 normal controls was downloaded from the Gene Expression Omnibus (GEO) database and differentially expressed genes (DEGs) were identified with Student's t-test. KEGG functional enrichment analysis was performed to uncover their biological functions. Three global networks were established for immune system, signaling molecules and interactions and cancer genes. The DEGs were compared with the networks to observe their distributions and determine important key genes, pathways and modules. Results: A total of 945 DEGs were obtained, 272 up-regulated and 673 down-regulated. KEGG analysis revealed that two groups of pathways were significantly enriched: immune function and signaling molecules and interactions. Following interaction network analysis further confirmed the association of DEGs in immune system, signaling molecules and interactions and cancer genes. Conclusions: Our study could systemically characterize gene expression changes in DLBCL with microarray technology. A range of key genes, pathways and function modules were revealed. Utility in diagnosis and treatment may be expected with further focused research.

On the Ultra-Wideband (UWB) System with Transmitted Reference Systems Receiver over Frequency Selective Fading

Joy Iong-Zong Chen,Li-Jung Liu,Wei Ming Huang 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7

In this paper the average transmitted reference receiver (ATRR) and simple transmitted reference receiver (STRR), of transmitted reference system receiver of UWB system are analyzed via the test decision of extend maximum likelihood ratio (EMLR) method.