Three dimensional reconstruction and measurement of underwater spent fuel assemblies

Zhao Jianping,He Shengbo,Yang Li,Feng Chang,Wu Guoqiang,Cai Gen 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.10

It is an important work to measure the dimensions of underwater spent fuel assemblies in the nuclear power industry during the overhaul, to judging whether the spent fuel assemblies can continue to be used. In this paper, a three dimensional reconstruction method for underwater spent fuel assemblies of nuclear reactor based on linear structured light is proposed, and the topography and size measurement was carried out based on the reconstructed 3D model. Multiple linear structured light sensors are used to obtain contour size data, and the shape data of the whole spent fuel assembly can be collected by onedimensional scanning motion. In this paper, we also presented a corrected model to correct the measurement error introduced by lead-glass and water is corrected. Then, we set up an underwater measurement system for spent fuel assembly based on this method. Finally, an underwater measurement experiment is carried out to verify the 3D reconstruction ability and measurement ability of the system, and the measurement error is less than ±0.05 mm.

Evaluation of thermal comfort in air-conditioned rooms based on structure/control-related parameters and data-mining method

Zhao Shunan,He Lin,Wu Xin,Xu Guowen,Xie Junlong,Cai Shanshan 대한설비공학회 2023 International Journal of Air-Conditioning and Refr Vol.31 No.1

Evaluating the thermal environment and thermal comfort in an air-conditioned room is an essential for estimating the performance of air-conditioning systems. However, multiple component structures and control-related parameters often lead to a long test cycle and large number of tests, significantly affecting the testing efficiency and speed. To address these problems, in this study, a data-mining method was proposed to predict and evaluate the thermal environment of an air-conditioned room. Owing to the limited amount of experimental data, the sample data were expanded by the simulation data of a collaborative platform between the air-conditioning system and air-conditioned room. Data-mining models, including the support vector regression (SVR), backpropagation (BP), and multiple linear regression (MLR) models, were developed and achieved good accuracy in evaluating the thermal environment by considering air-conditioning systems with various structures and control parameters. In the multiple-input single-output evaluation method, the prediction accuracy of the SVR model was higher than those of the BP and MLR models with respect to the vertical air temperature difference, temperature uniformity, temperature drop rate, and draft rate, while the result was the opposite in terms of the predicted mean vote indices. In the multiple-input multiple-output evaluation method, there was a decline in prediction accuracy and an increase in efficiency prediction compared with multiple-input single-output evaluation.

Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway

Cai Zhaowei,He Shaojuan,Li Tao,Zhao Li,Zhang Kerong 한국통합생물학회 2020 Animal cells and systems Vol.24 No.4

Polycystic ovary syndrome (PCOS) is recognized as a general endocrine disease and reproductive disorder. Although evidence indicates that PCOS has a complex etiology and genetic basis, the pathogenic mechanisms and signal pathway in PCOS remain unclear. In this study, the normal structure of follicle and corpus luteum were observed, and no cyst nor hyperemia was observed under the light microscopic study with hematoxylin and eosin (H&E) staining. Eestosterone and progesterone were evaluated by radioimmunoassay in rat serum. The alterations of proliferative ability and cell cycle distribution of each group were assessed by Cell Counting Kit-8 (CCK8) assay and flow cytometry. The protein expression of p-mTOR/mTOR, p-PI3K/PI3K, p-AKT/AKT, and GAPDH were analyzed by western blotting. Both doses of PLB could benefit the ovarian morphology and polycystic property. PLBinduced a suppress effect on the proliferation of rat ovarian granulosa cells. In addition, PLB also induced concentrationdependent apoptosis in rat ovarian granulosa cells. The rat ovarian granulosa cells treated with PLB that the expression levels of p-AKT, p-mTOR, and p-PI3K were significantly decreased in a concentration-dependent manner. PLB not only plays a critical role in attenuating the pathology and polycystic property changes in the ovary but can also induce rat ovarian granulosa cell apoptosis through the PI3K/Akt/mTOR signal pathway. This study showed the innovative role of PLB in the pathogenesis of PCOS and provides a new therapeutic modality for the treatment of PCOS.

In-situ preparation of WO_3/TiO_2 composite film with increased photo quantum efficiency on titanium substrate

J. He,Q.Z. Cai,D. Zhu,Q. Luo,D.Q. Zhang,X.W. Li,X. Zhao,W. Sun 한국물리학회 2011 Current Applied Physics Vol.11 No.1

A WO_3/TiO_2 composite film was in-situ prepared in tungstate electrolyte and characterized by Electron microscopy, X-ray diffraction, UV―Vis spectrophotometer and Photoluminescence. The oxide film consists of mixture phases of anatase and rutile, showing porous structures and metallurgical bond with substrate. With the incorporation of WO_3 into TiO_2, the optical absorption edge of TiO_2 filmwas shifted to longer wavelength slightly and the separation between electrons and holes was promoted effectively. WO_3/TiO_2 composite film with increased photo quantum efficiency will be promising in the treatment of environmental pollutants.

Line-Interactive UPS for Low-Voltage Microgrids

Zhang, Ping,Cai, Huanyu,Zhao, Hengyang,Shi, Jianjiang,He, Xiangning The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.6

Line-interactive uninterruptible power supply (UPS) systems are good candidates for providing energy storage within a microgrid. In this paper, a control scheme for a line-interactive UPS system applied in a low-voltage microgrid is presented. It is based on the Q-w and P-E droop control to achieve a seamless transition between grid-connected and stand-alone operation modes. Moreover, a new model for designing the controllers is built in the dq-frame based on the instantaneous power definition. The new-built model takes into account the dynamic performance of the output impedance of the inverter in the dq-frame and can be evaluated in the time domain. Compared to the traditional model based on the instantaneous power definition, the new-built model is more accurate to describe the dynamic performance of the system. Simulation and experimental results obtained with a microgrid consisting of two 40-kW line-interactive UPS systems are given to validate the control strategy of the line-active UPS system and the accuracy of the new-built model.

Epigenetic silencing of olfactomedin-4 enhances gastric cancer cell invasion via activation of focal adhesion kinase signaling

( Li Li Guo ),( Zhao Cai He ),( Chang Qing Yang ),( Pei Tang Qiao ),( Guo Ling Yin ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.11

Downregulation of olfactomedin-4 (OLFM4) is associated with tumor progression, lymph node invasion and metastases. However, whether or not downregulation of OLFM4 is associated with epigenetic silencing remains unknown. In this study, we investigate the role of OLFM4 in gastric cancer cell invasion. We confirm the previous result that OLFM4 expression is increased in gastric cancer tissues and decreases with an increasing number of metastatic lymph nodes, which are associated with OLFM4 promoter hypermethylation. Overexpression of OLFM4 in gastric cancer cells had an inhibitory effect on cell invasion. Furthermore, we found that focal adhesion kinase (FAK) was negatively correlated with OLFM4 in regards to lymph node metastasis in gastric cancer tissues. Also, inhibition of FAK induced by OLFM4 knockdown resulted in a decrease in cell invasion. Thus, our study demonstrates that epigenetic silencing of OLFM4 enhances gastric cancer cell invasion via activation of FAK signaling. [BMB Reports 2015; 48(11): 630-635]

Line-Interactive UPS for Low-Voltage Microgrids

Ping Zhang,Huanyu Cai,Hengyang Zhao,Jianjiang Shi,Xiangning He 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.6

Line-interactive uninterruptible power supply (UPS) systems are good candidates for providing energy storage within a microgrid. In this paper, a control scheme for a line-interactive UPS system applied in a low-voltage microgrid is presented. It is based on the Q-w and P-E droop control to achieve a seamless transition between grid-connected and stand-alone operation modes. Moreover, a new model for designing the controllers is built in the dq-frame based on the instantaneous power definition. The new-built model takes into account the dynamic performance of the output impedance of the inverter in the dq-frame and can be evaluated in the time domain. Compared to the traditional model based on the instantaneous power definition, the new-built model is more accurate to describe the dynamic performance of the system. Simulation and experimental results obtained with a microgrid consisting of two 40-kW line-interactive UPS systems are given to validate the control strategy of the line-active UPS system and the accuracy of the new-built model.

Maternal Low-protein Diet Alters Ovarian Expression of Folliculogenic and Steroidogenic Genes and Their Regulatory MicroRNAs in Neonatal Piglets

Sui, Shiyan,Jia, Yimin,He, Bin,Li, Runsheng,Li, Xian,Cai, Demin,Song, Haogang,Zhang, Rongkui,Zhao, Ruqian Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.12

Maternal malnutrition during pregnancy may give rise to female offspring with disrupted ovary functions in adult age. Neonatal ovary development predisposes adult ovary function, yet the effect of maternal nutrition on the neonatal ovary has not been described. Therefore, here we show the impact of maternal protein restriction on the expression of folliculogenic and steroidogenic genes, their regulatory microRNAs and promoter DNA methylation in the ovary of neonatal piglets. Sows were fed either standard-protein (SP, 15% crude protein) or low-protein (LP, 7.5% crude protein) diets throughout gestation. Female piglets born to LP sows showed significantly decreased ovary weight relative to body weight (p<0.05) at birth, which was accompanied with an increased serum estradiol level (p<0.05). The LP piglets demonstrated higher ratio of bcl-2 associated X protein/B cell lymphoma/leukemia-2 mRNA (p<0.01), which was associated with up-regulated mRNA expression of bone morphogenic protein 4 (BMP4) (p<0.05) and proliferating cell nuclear antigen (PCNA) (p<0.05). The steroidogenic gene, cytochrome P450 aromatase (CYP19A1) was significantly down-regulated (p<0.05) in LP piglets. The alterations in ovarian gene expression were associated with a significant down-regulation of follicle-stimulating hormone receptor mRNA expression (p<0.05) in LP piglets. Moreover, three microRNAs, including miR-423-5p targeting both CYP19A1 and PCNA, miR-378 targeting CYP19A1 and miR-210 targeting BMP4, were significantly down-regulated (p<0.05) in the ovary of LP piglets. These results suggest that microRNAs are involved in mediating the effect of maternal protein restriction on ovarian function through regulating the expression of folliculogenic and steroidogenic genes in newborn piglets.

Directed Evolution of Beta-galactosidase from Escherichia coil into Beta-glucuronidase

( Ai Sheng Xiong ),( Ri He Peng ),( Jing Zhuang ),( Jin Ge Liu ),( Fang Xu ),( Bin Cai ),( Zhao Kui Guo ),( Yu Shan Qiao ),( Jian Min Chen ),( Zhen Zhang ),( Quan Hong Yao ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.3

Studies of the effects and mechanisms of ginsenoside Re and Rk₃ on myelosuppression induced by cyclophosphamide

Han, Jiahong,Xia, Jing,Zhang, Lianxue,Cai, Enbo,Zhao, Yan,Fei, Xuan,Jia, Xiaohuan,Yang, He,Liu, Shuangli The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.4

Background: Ginsenoside Re (Re) is one of the major components of Panax ginseng Meyer. Ginsenoside $Rk_3$ ($Rk_3$) is a secondary metabolite of Re. The aim of this study was to investigate and compare the effects and underlying mechanisms of Re and $Rk_3$ on cyclophosphamide-induced myelosuppression. Methods: The mice myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide. Peripheral blood cells, bone marrow nucleated cells, and colony yield of hematopoietic progenitor cells in vitro were counted. The levels of erythropoietin, thrombopoietin, and granulocyte macrophage colony-stimulating factor in plasma were measured by enzyme-linked immunosorbent assay. Bone marrow cell cycle was performed by flow cytometry. The expression of apoptotic protein bcl-2, bax, and caspase-3 was detected by Western blotting. Results: Both Re and $Rk_3$ could improve peripheral blood cells, bone marrow nucleated cell counts, thymus index, and spleen index. Furthermore, they could enhance the yield of colonies cultured in vitro and make the levels of granulocyte macrophage colony-stimulating factor, erythropoietin, and thrombopoietin normal, reduce the ratio of $G_0/G_1$ phase cells, and increase the proliferation index. Finally, Re and $Rk_3$ could upregulate the expression of bcl-2, whereas they could downregulate the expression of bax and caspase-3. Conclusion: Re and $Rk_3$ could improve the hematopoietic function of myelosuppressed mice. The effect of $Rk_3$ was superior to that of Re at any dose. Regulating the levels of cytokines, promoting cells enter the normal cell cycle, regulating the balance of bcl-2/bax, and inhibiting the expression of caspase-3 may be the effects of Re and $Rk_3$ on myelosuppression.