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( Yong Cheng Jin ),( Jeng A Han ),( Cheng Xiong Xu ),( Sang Kee Kang ),( Sang Hun Kim ),( Kang Suk Seo ),( Du Hak Yoon ),( Yun Jaie Choi ),( Hong Gu Lee ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.2
The aim of this study was to investigate protein profiles related to the induction of adipogenesis within the bovine longissimus dorsi muscle (BLDM) by proteomic analysis. We analyzed BLDM proteins at different growth stages to clarify the physiological mechanisms of marbled muscle development in 20 head of Korean native cattle (11 month: 10 head, 17 month: 10 head). BLDM proteins were analyzed by two-dimensional electrophoresis and image analysis. Villin 2 was specifically identified by mass spectrometry and a protein search engine. Villin 2 protein expression in BLDM decreased during the fat development stage in test steers. In a Western blot cell culture study of spontaneously immortal bovine muscle fibroblasts, the abundance of Villin 2 was shown to be down-regulated during differentiation into muscle. In 3T3-L1 mouse embryonic fibroblasts, Villin 2 was decreased during differentiation into adipocytes. The results suggest that Villin 2 may be related to the induction of transdifferentiation and adipogenesis in bovine longissimus dorsi muscle. [BMB reports 2012; 45(2): 102-107].
( Seung Soo Yoo ),( Cheng Cheng Jin ),( Jin Eun Choi ),( Keum Ju Choi ),( So Yeon Lee ),( Yong Dae Lee ),( Shin Yup Lee ),( Jae Hee Lee ),( Seung Ick Cha ),( Chang Ho Kim ),( Jae Yong Park ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-
Background: ENCODE project revealed that nearby or distantly located non-coding DNA regulates the expression of coding genes. RegulomeDB is a new database that can be used to predict whether variants affect transcription factor binding and gene expression. We investigated the association between lung cancer risk and potentially functional polymorphisms of XRCC1 that were selected using RegulomeDB in a Korean population. Methods: 185 polymorphisms of XRCC1 were evaluated using RegulomeDB. There was strong evidence that 10 polymorphisms affected XRCC1 expression with scores of 1a-1f that were based on the RegulomeDB. rs2854510 polymorphism was rare in Asians. Eight polymorphisms were in strong linkage disequilibrium (LD). rs2854509, which was one of the 8 polymorphisms in LD, and rs7248167, which was not in the LD block, were genotyped using the Taq-Man® assay in 610 lung cancer patients and 607 age- and sex-matched controls. Additionally, 4 polymorphisms of XRCC1 (Arg399Gln, Arg280His, Arg194Trp, and -77T>C), which were investigated with regard to the association with lung cancer risk in previous studies, were also genotyped. Results: Two polymorphisms (rs2854509 and rs7248167) that were predicted to affect XRCC1 expression based on their RegulomeDB scores were not associated with lung cancer risk (P = 0.31 and 0.93, respectively). When stratified according to age, gender, smoking status, and tumor histology, the 2 polymorphisms of XRCC1 were not associated with lung cancer risk. Among the 4 polymorphisms that were previously studied, only XRCC1 Arg280His was significantly associated with lung cancer risk (dominant model, adjusted odds ratio = 0.61, 95 % confidence interval = 0.46-0.83, P = 0.002). Conclusions: Although RegulomeDB is an attractive tool for predicting regulatory potential of variants, the 2 polymorphisms that were selected using RegulomeDB were not associated with lung cancer risk.
Miller Decoding Algorithm Using Sample Recovery and Correlation Method for UHF Gen2 RFID System
Cheng Jin,Ki Yong Jeon,Sung Ho Cho 대한전자공학회 2008 ITC-CSCC :International Technical Conference on Ci Vol.2008 No.7
In this paper, by analyzing the characteristics of the received signal in both practical and theory environment, a Miller decoding algorithm basing on a sample recovery method and correlation concept is designed. The sample recovery method is designed to apply on the samples of the received signal which often include some degree of distortions caused by noise or timing jitter. Basing on the concept of matched filter, which is obtained by correlating the received signal with a known reference signal to detect the presence of the data symbol in the received signal, a Miller decoding algorithm utilizing correlation is designed. Together with the signal recovery method, the Miller decoding algorithm using correlation shows a good decoding performance under condition of timing jitter and high signal corruption rate caused by noise.
Jin-cheng Zhou,Quanquan Liu,Yong-xu Han,Hui Dong 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3
Due to ongoing climate change, short-term extreme heat waves in the summer are expected to be more frequent. Insect eggs are sensitive to thermal stress. This raises the question of whether herbivore insects' thermal adaptability would be changed after a single extreme heat wave at the egg stage. In this study, we examined the developmental performance of Ostrinia furnacalis Guenée at 25 °C, 27 °C, 29 °C or 31 °C after a single extreme heat wave (42 °C) for 0 h (control), 1 h, 2 h, or 3 h at the egg stage. The results showed that O. furnacalis at the egg or larval stage was more sensitive to a single heat wave than it was at the pupae or adult stage. After a single heat wave, O. furnacalis showed a reduced egg-hatching rate or reduced larval survival rate, but the optimum temperature for egg hatching and larval survival was higher than that in the control. The upper temperature threshold and optimum temperature for larval development in the control were higher than that after a single extreme heat wave. Both male and female pupal weight decreased with increasing temperature, and pupal weight decreased faster in females than in males. The Cox proportional hazard model showed that when O. furnacalis developed at 25 °C, the instantaneous death risk of adults with a 3 h heat wave at the egg stage was higher than that of the control, but when O. furnacalis developed at 29 °C and 31 °C, the instantaneous death risk of adults after a heat wave was significantly lower than that of the control. Our study highlights the effect of a single heat wave on O. furnacalis eggs and the subsequent development of survival individuals.
Backward Orientation Tracking Control of Mobile Robot with N Trailers
Jin Cheng,Bin Wang,Yong Zhang,Zhonghua Wang 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.2
A feedback control law for orientation tracking problem of a mobile robot with N on-axle trailers isproposed in this paper. Based on a recursive designing process, the orientation tracking problem is converted intostabilizing the including angles on the designed reference value. The designing process of the control law is simpleand can be easily extended to any given N trailers system. With input-to-state stability on cascade form system, theclosed-loop system is proven to be asymptotically stable. Simulation experiment on a mobile robot with 4 trailersis implemented and the results demonstrate that the proposed control law works successfully.
Cheng, Yan Fen,Jin, Wei,Mao, Sheng Yong,Zhu, Wei-Yun Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.10
The metabolomic profile of the anaerobic fungus Piromyces sp. F1, isolated from the rumen of goats, and how this is affected by the presence of naturally associated methanogens, was analyzed by nuclear magnetic resonance spectroscopy. The major metabolites in the fungal monoculture were formate, lactate, ethanol, acetate, succinate, sugars/amino acids and ${\alpha}$-ketoglutarate, whereas the co-cultures of anaerobic fungi and associated methanogens produced citrate. This is the first report of citrate as a major metabolite of anaerobic fungi. Univariate analysis showed that the mean values of formate, lactate, ethanol, citrate, succinate and acetate in co-cultures were significantly higher than those in the fungal monoculture, while the mean values of glucose and ${\alpha}$-ketoglutarate were significantly reduced in co-cultures. Unsupervised principal components analysis revealed separation of metabolite profiles of the fungal mono-culture and co-cultures. In conclusion, the novel finding of citrate as one of the major metabolites of anaerobic fungi associated with methanogens may suggest a new yet to be identified pathway exists in co-culture. Anaerobic fungal metabolism was shifted by associated methanogens, indicating that anaerobic fungi are important providers of substrates for methanogens in the rumen and thus play a key role in ruminal methanogenesis.
( Jin Won Hyun ),( Ji Won Cha ),( Mei Jing Piao ),( Ki Cheon Kim ),( Cheng Wen Yao ),( Jian Zheng ),( Seong Min Kim ),( Chang Lim Hyun ),( Yong Seok Ahn ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.2
We investigated the protective effects of chlorogenic acid (CGA), a polyphenol compound, on oxidative damage induced by UVBexposure on human HaCaT cells. In a cell-free system, CGA scavenged 1,1-diphenyl-2-picrylhydrazyl radicals, superoxide anions,hydroxyl radicals, and intracellular reactive oxygen species (ROS) generated by hydrogen peroxide and ultraviolet B (UVB). Furthermore, CGA absorbed electromagnetic radiation in the UVB range (280-320 nm). UVB exposure resulted in damage to cellularDNA, as demonstrated in a comet assay; pre-treatment of cells with CGA prior to UVB irradiation prevented DNA damage andincreased cell viability. Furthermore, CGA pre-treatment prevented or ameliorated apoptosis-related changes in UVB-exposedcells, including the formation of apoptotic bodies, disruption of mitochondrial membrane potential, and alterations in the levelsof the apoptosis-related proteins Bcl-2, Bax, and caspase-3. Our findings suggest that CGA protects cells from oxidative stressinduced by UVB radiation.