Impact of tillage practices on soil bacterial diversity and composition under the tobacco-rice rotation in China

Yanping Lei,Yongliang Xiao,Lifeng Li,Chaoqiang Jiang,Chaolong Zu,Tian Li,Hui Cao 한국미생물학회 2017 The journal of microbiology Vol.55 No.5

Tobacco-rice rotation is a common farming system in southChina, and many tillage practices such as straw mulching, dolomitedust, and quicklime application have been adopted toimprove crop production. These agricultural managementpractices alter soil physical and chemical properties and affectmicrobial life environment and community composition. Inthis research, six tillage practices including no tobacco andrice straw mulching (CK), tobacco and rice straw mulching(TrSr), rice straw returning fire (TrSc), tobacco and rice strawmulching with dolomite dust (TSD), rice straw returning fireand quicklime (TSQ), and rice straw returning fire, quicklimeand reduced fertilizer (TSQf) were conducted to detect changesin soil bacterial diversity and composition using Illumina sequencing. The results showed that the total number of operationaltaxonomic units (OTUs) from the six treatments was2030, and the number of mutual OTUs among all sampleswas 550. The TrSc treatment had the highest diversity andrichness, while TSQf had the lowest. Soil physio-chemicalproperties and microbial diversity can influence each other. Proteobacteria and Actinobacteria had the greatest proportionin all treatments. The abundance of Nitrospirae was thehighest in the TrSc treatment. The TSQf treatment had thehighest abundance of Firmicutes. The abundance of Nitrospirain the TrSc treatment was 2.29-fold over CK. Streptomycesaffiliated with Firmicutes improved by 37.33% in TSQf comparedto TSQ. TSQf treatment was considered to be the mostimportant factor in determining the relative abundance atthe genus level.

Novel Implementation for the LDPC-Decoders Based on Factor Graph

Li Yanping,Moon Ho Lee 대한전자공학회 2004 ITC-CSCC :International Technical Conference on Ci Vol.2004 No.7

High expression of HOXC6 predicts a poor prognosis and induces proliferation and inflammation in multiple myeloma cells

Li Zhihua,Wang Yaru,Hou Xiaoxu,Guo Luyao,Li Yanling,Ma Yanping,Ma Yanping 한국유전학회 2023 Genes & Genomics Vol.45 No.7

Background Multiple myeloma (MM) is a common blood system malignance accompanied by monoclonal plasma cell hyperplasia. Homeobox C6 (HOXC6) acts as an oncogene in various cancers, but its function on MM is elusive. Objective The role of HOXC6 on MM development was clarified in this study. Methods HOXC6 expression and its clinical significance were determined in the peripheral blood samples collected from forty MM patients and thirty healthy adult volunteers. The overall survival was evaluated by Kaplan-Meier analysis with the log-rank test. Cell viability, proliferation and apoptosis were measured by CCK-8, EdU assay and Flow cytometry in U266 and MM.1R cells. Tumor growth was estimated by a xenograft assay. The apoptosis of tumor tissues was evaluated using TUNEL staining. The protein level in tissues was tested by immunohistochemistry. Results The HOXC6 expression was elevated in MM and high HOXC6 level was associated with the poor overall survival of MM. Besides, the HOXC6 expression was associated with hemoglobin level and ISS stage. Furthermore, silencing HOXC6 suppressed cell proliferation, induced cell apoptosis, and restrained the secretion of inflammatory factors (TNF-α, IL-6, and IL-8) in MM cells through inactivating the NF-κB pathway. Moreover, silencing HOXC6 suppressed the tumor growth of MM, the inflammatory factors levels, and the activation of NF-κB pathway but enhanced apoptosis in vivo. Conclusion HOXC6 was elevated in MM and associated with poor survival. Knockdown of HOXC6 suppressed proliferation, inflammation and tumorigenicity of MM cells via inactivating the NF-κB pathway. HOXC6 may be a meaningful target for MM therapy.

Three-vector-based model predictive current control with disturbance feedforward compensation

Xu, Yanping,Li, Hangke,Ren, Jinglu,Zhang, Yanping The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.3

Finite control set model predictive current control (FCS-MPCC) has attracted the attention of a large number of researchers due to its intuitive idea, fast dynamic response and control objective flexibility. However, FCS-MPCC has a high steady-state current ripple. Three-vector-based model predictive current control (TV-MPCC) is an effective method to improve steady-state performance. However, it results in high motor parameter dependency. In this paper, the effect of motor parameters mismatch in FCS-MPCC has been analyzed. The control performance of FCS-MPCC is impacted when the motor parameters are mismatched. Then based on the disturbance estimation technique, a disturbance feedforward compensation based generalized proportional integral observer is used to reduce the disturbances and improves the robustness performance of the system. Experimental results show that the proposed method effectively improve the robustness of the system.

The Complete Oxidation of Ethanol at Low Temperature over a Novel Pd–Ce/γ-Al2O3–TiO2 Catalyst

Yanping Wang,Jinshuang Zhao,Xiaoli Wang,Zhe Li,Pengfei Liu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.8

Pd–Ce/γ-Al2O3–TiO2 catalysts were prepared by combined sol–gel and impregnation methods. Transmission electron microscopy, X-ray diffraction, H2-temperature-programmed reduction, O2-temperature-programmed desorption, and ethanol oxidation experiments were conducted to determine the properties of the catalysts. Addition of an optimal amount of Ce improved the performance of the Pd/γ-Al2O3–TiO2 catalyst in promoting the complete oxidation of ethanol. The catalyst with 1% Ce exhibited the highest activity, and catalyzed complete oxidation of ethanol at 175 °C; its selectivity to CO2 reached 87%. Characterization results show that addition of appropriate amount of Ce could enrich the PdO species, and weaken the Pd–O bonds, thus enhancing oxidation ability of the catalyst. Meanwhile, the introduction of CeO2 could make PdO better dispersed on γ-Al2O3–TiO2, which is beneficial for the improvement of the catalytic oxidation activity.

Robust Predictive Speed Control for SPMSM Drives Based on Extended State Observers

Yanping Xu,Yongle Hou,Zehui Li 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.2

The predictive speed control (PSC) strategy can realize the simultaneous control of speed and current by using one cost function. As a model-based control method, the performance of the PSC is vulnerable to model mismatches such as load torque disturbances and parameter uncertainties. To solve this problem, this paper presents a robust predictive speed control (RPSC) strategy for surface-mounted permanent magnet synchronous motor (SPMSM) drives. The proposed RPSC uses extended state observers (ESOs) to estimate the lumped disturbances caused by load torque changes and parameter mismatches. The observer-based prediction model is then compensated by using the estimated disturbances. The introduction of ESOs can achieve robustness against predictive model uncertainties. In addition, a modified cost function is designed to further suppress load torque disturbances. The performance of the proposed RPSC scheme has been corroborated by experimental results under the condition of load torque changes and parameter mismatches.

Apoptosis Progression in the Hair Cells in the Organ of Corti of GJB2 Conditional Knockout Mice

Yanping Zhang,Xiaoqiang Zhang,Lina Li,Yurui Sun,Jianhe Sun 대한이비인후과학회 2012 Clinical and Experimental Otorhinolaryngology Vol.5 No.3

Objectives. Apoptosis may play an important role in the mechanism underlying the GJB2 gene conditional knockout (cCx26) mice cochlear cell death. The objective of this study was to explore the the damage mode of the outer hair cells (OHCs) and its real time point of apoptosis and provide information to further explore the role of apoptosis in the happening of hearing loss in cCx26 mice. Methods. Cochleae from mice at various developmental stages (P8, P12, and P21) were dissected out and first used to be observed under the scanning electron microscope (SEM). Basilar membranes from mice at P8, P14, P18, and P21were stained by fluorescein isothiocyanate-conjugated phalloidin and propidium iodide (PI) and examined under confocal microscope. Results. The loss of OHCs of cCx26 knockout mice was first set between P12 and P21 under SEM. Whole mount phalloidin and PI staining revealed that obvious apoptotic appearance of the OHCs surface morphology was observed at P18. Conclusion. Typical apoptotic morphology was found in the OHCs in the organ of Corti of the cCx26 mice at P18. This may provide information to further study the role of apoptosis in the occurrence of hearing loss of cCx26 mice. Objectives. Apoptosis may play an important role in the mechanism underlying the GJB2 gene conditional knockout (cCx26) mice cochlear cell death. The objective of this study was to explore the the damage mode of the outer hair cells (OHCs) and its real time point of apoptosis and provide information to further explore the role of apoptosis in the happening of hearing loss in cCx26 mice. Methods. Cochleae from mice at various developmental stages (P8, P12, and P21) were dissected out and first used to be observed under the scanning electron microscope (SEM). Basilar membranes from mice at P8, P14, P18, and P21were stained by fluorescein isothiocyanate-conjugated phalloidin and propidium iodide (PI) and examined under confocal microscope. Results. The loss of OHCs of cCx26 knockout mice was first set between P12 and P21 under SEM. Whole mount phalloidin and PI staining revealed that obvious apoptotic appearance of the OHCs surface morphology was observed at P18. Conclusion. Typical apoptotic morphology was found in the OHCs in the organ of Corti of the cCx26 mice at P18. This may provide information to further study the role of apoptosis in the occurrence of hearing loss of cCx26 mice.

Effect of control route on the unstart/restart characteristics of an over-under TBCC inlet

Li, Nan,Chang, Juntao,Tang, Jingfeng,Yu, Daren,Bao, Wen,Song, Yanping Techno-Press 2018 Advances in aircraft and spacecraft science Vol.5 No.4

Numerical simulations have been conducted to study the unstart/restart characteristics of an over-under turbine-based combined-cycle propulsion system (TBCC) inlet during the inlet transition phase. A dual-solution area exists according to the Kantrowitz theory, in which the inlet states may be different even with the same input parameters. The entire transition process was divided into five stages and the unstart/restart hysteresis loop for each stage was also obtained. These loops construct a hysteresis surface which separates the operating space of the engine into three parts: in which a) inlet can maintain a started state; b) inlet keeps an unstarted state; c) inlet state depends on its initial state. During the transition, the operation of the engine follows a certain order with different backpressures and splitter angles, namely control route, which may result in disparate inlet states. Nine control routes with different backpressures and transition stages were designed to illuminate the route-dependent behavior of the inlet. The control routes operating towards the unstart boundary can make the inlet transit from a started state into an unstarted one. But operating backward the same route cannot make the inlet restart, additional effort should be made.

Thermal, Mechanical and Rheological Properties of Poly(lactic acid) Chain Extended with Polyaryl Polymethylene Isocyanate

Yanping Hao,Yi Li,Zhigang Liu,Xiangyu Yan,Yi Tong,Huiliang Zhang 한국섬유공학회 2019 Fibers and polymers Vol.20 No.9

In this study, polyaryl polymethylene isocyanate (PAPI) was used as a chain extender for poly(lactic acid) (PLA)to produce a high molecular weight material with better rheological, thermal and mechanical properties. The reactionbetween PLA chains and PAPI was proved by FTIR during reactive blending. The results showed that the molecular weightand molecular weight distribution were increased with the addition of PAPI content due to the chain extension. Chainextension was also responsible for the increased modulus and complex viscosity. The glass transition temperature (Tg) andthermal stability increased by incorporating with PAPI. The results of mechanical properties showed that a considerablyhigher tensile strength and Young’s modulus of the reactive blends compared with neat PLA.

An Improved Tet-on System to Tightly Conditionally Regulate Reporter Gene Expression

Yanping Ren,Xiangping Li,Qingyou Liu,Yanfei Deng,Deshun Shi 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

Reporter genes are often used as markers totrack the integration and expression of target genes inanimal genetic engineering. To avoid potential side effectsfrom reporter genes, in this study an improved Tet-onsystem was developed to control reporter gene expression,and its effectiveness was explored in transgenic cells. First,the rtTA protein was fused with Tat and NLS proteins toobtain the prokaryotic expression vector pET32a-Tat-rtTANSL. A eukaryotic transgenic vector was constructed, p-HS4-BPA-TmA-HS4, in which the reporter (mCherry) andtarget (PRL) genes were promoted by TRE and BCN,respectively. After confirming the functionality of thetransgenic vector, purified rtTA protein and Dox wereadded to induce expression of the mCherry gene. Theoptimal amount of purified rtTA protein, its influence ontarget gene expression, and the time of rtTA protein actionwere each investigated separately. The results showed thatrtTA protein was expressed in transformed E. coli with IPTGinduction. TRE could promote mCherry gene expressionby co-transfecting the constructed transgenic vector andprtTA plasmid. When purified rtTA protein and Dox wereadded, red fluorescence was observed in Bcap-37 cellstransfected with the p-HS4-BPA-TmA-HS4 vector, and theexogenous PRL gene was expressed regardless of mCherrygene expression. The optimal amount of rtTA protein was16 μg/mL, and it needed about 6 h to promote mCherrygene expression in transfected cells. These resultsdemonstrate that the expression of the mCherry reportergene can be tightly and conditionally regulated by our Tetonsystem.