The USP21/YY1/SNHG16 axis contributes to tumor proliferation, migration, and invasion of non-small-cell lung cancer

Pei Xu,Haibo Xiao,Qi Yang,Rui Hu,Lianyong Jiang,Rui Bi,Xueyan Jiang,Lei Wang,Ju Mei,Fangbao Ding,Jianbing Huang 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Deubiquitinases (DUBs) and noncoding RNAs have been the subjects of recent extensive studies regarding their roles in lung cancer, but the mechanisms involved are largely unknown. In our study, we used The Cancer Genome Atlas data set and bioinformatics analyses and identified USP21, a DUB, as a potential contributor to oncogenesis in nonsmall-cell lung cancer (NSCLC). We further demonstrated that USP21 was highly expressed in NSCLCs. We then conducted a series of in vitro and in vivo assays to explore the effect of USP21 on NSCLC progression and the underlying mechanism involved. USP21 promoted NSCLC cell proliferation, migration, and invasion and in vivo tumor growth by stabilizing a well-known oncogene, Yin Yang-1 (YY1), via mediating its deubiquitination. Furthermore, YY1 transcriptionally regulates the expression of SNHG16. Moreover, StarBase bioinformatics analyses predicted that miR4500 targets SNHG16 and USP21. A series of in vitro experiments indicated that SNHG16 increased the expression of USP21 through miR-4500. In summary, the USP21/YY1/SNHG16 axis plays a role in promoting the progression of NSCLC. Therefore, the USP21/YY1/SNHG16/miR-4500 axis may be a potential therapeutic target in NSCLC treatment.

마그네슘 풍부 해양미네랄 용액이 hairless 마우스의 아토피성 피부염에 미치는 영향

김동희,이규재,최주봉,이영미,윤양숙,김정례,장병수,양용석 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

Atopic dermatitis (AD) is a chronically relapsing inflammatory skin disease that often has asthma and allergic rhinitis. Magnesium salts, the important component of minerals in Dead Sea water, are known to exhibit beneficial effects in inflammatory disease. Favorable effects of magnesium ions and sea water treated to the skin of patients with contact dermatitis have been reported. But histological and immunological investigations are insufficient. This study was performed to examine the inhibitory effect of magnesium-rich sea mineral water on the development of AD-like skin lesions in hairless mice. AD-like skin lesions are induced by the repeated application of 2,4-dinitrochlorobenzene (DNCB). Local application of magnesium-rich sea mineral water on hairless mice skin applied with DNCB inhibited the development of AD-like skin lesions as exemplified by a significant increase in skin hydration (p<0.01), and a decrease in epidermal water loss (p<0.01). Serum IgE level was also significantly decreased (p<0.01). These results suggest that magnesiumrich sea mineral water inhibits the development of DNCB-induced AD-like skin lesions in hairless mice. These observations indicate that magnesium-rich sea mineral water may be alternative and assistant substances for the management of AD. 아토피성 피부염은 주로 천식과 비염 등을 동반하는, 주위에서 흔히 볼 수 있는 만성 염증성 피부질환으로 유전학적, 환경적, 면역학적 요인이 복잡하게 연관되어 발병한다. 해수에 포함된 마그네슘염은 피부에 작용하여 피부장벽을 보호하는 것으로 알려지고 그에 대한 면역학적인 연구와 조직학적 연구는 아직 부족한 실정이다. 이번 연구에서는 피부염을 인위적으로 일으키는 hapten 형성물질인 DNCB를 hairless mice에 도포하여 아토피 피부염 동물 모델로 만든 후, 마그네슘이 다량 함유된 해양 미네랄수를 처리한 후 피부장벽에 미치는 영향을 관찰하였다. DNCB로 피부염을 유발한 hairless mice에 해양미네랄수를 국소적으로 도포하였을 때 유의한 피부수분함량이 증가와 경피수분손실의 감소를 확인하였다 (p<0.01). 피부측정에서 피부거칠기(skin roughness, p<0.05)와 스케일생성 (skin scaliness, p<0.01)은 실험군에서 유의한 개선효과를 나타내었으며 조직학적 검사에서도 피부손상지수의 유의한 감소 (p<0.01)와 비만세포와 (p<0.01) 호산구의 감소(p<0.05) 소견을 보였고 또한 혈청 IgE의 감소를 관찰할 수 있었다(p<0.01). 이상과 같이 마그네슘이 다량 함유된 해양 미네랄수 도포는 피부장벽의 손상을 줄이고 피부수분손실을 효과적으로 줄임으로 아토피성 피부염 증상 유발을 억제할수 있음을 확인하였다. 현재까지 아토피성 피부염의 관리를 위하여 세라마이드나 식물성 오일의 보습제가 주로 활용되고 있는 상황에서 부가적인 피부장벽의 보호를 위하여 탈염 해양 미네랄수의 활용이 가능할 것으로 판단되며 장기적으로 아토피 피부염치료의 대체, 혹은 보조적 물질로 활용될 수 있을 것으로 기대된다.

MLL4 Regulates the Progression of Non-Small-Cell Lung Cancer by Regulating the PI3K/AKT/SOX2 Axis

Yang Yang,Rongfang Qiu,Qiaoyou Weng,Ziwei Xu,Jingjing Song,Siyu Zhao,Miaomiao Meng,Dengke Zhang,Chunli Kong,Hailin Wang,Min Xu,Zhongwei Zhao,Jiansong Ji 대한암학회 2023 Cancer Research and Treatment Vol.55 No.3

Purpose Mixed-lineage leukemia protein 4 (MLL4/KMT2D) is a histone methyltransferase, and its mutation has been reported to be associated with a poor prognosis in many cancers, including lung cancer. We investigated the function of MLL4 in lung carcinogenesis. Materials and Methods RNA sequencing (RNA-seq) in A549 cells transfected with control siRNA or MLL4 siRNA was performed. Also, we used EdU incorporation assay, colony formation assays, growth curve analysis, transwell invasion assays, immunohistochemical staining, and in vivo bioluminescence assay to investigate the function of MLL4 in lung carcinogenesis. Results We found that MLL4 expression was downregulated in non–small cell lung cancer (NSCLC) tissues compared to adjacent normal tissues and tended to decrease with disease stage progression. We analyzed the transcriptomes in control and MLL4- deficient cells using high-throughput RNA deep sequencing (RNA-seq) and identified a cohort of target genes, such as SOX2, ATF1, FOXP4, PIK3IP1, SIRT4, TENT5B, and LFNG, some of which are related to proliferation and metastasis. Our results showed that low expression of MLL4 promotes NSCLC cell proliferation and metastasis and is required for the maintenance of NSCLC stem cell properties. Conclusion Our findings identify an important role of MLL4 in lung carcinogenesis through transcriptional regulation of PIK3IP1, affecting the PI3K/AKT/SOX2 axis, and suggest that MLL4 could be a potential prognostic indicator and target for NSCLC therapy.

QTL Analysis for Low-Temperature Requirement Vernalization in Brassica campestris

Yang Xu,Yu Yang Jun,Zhang Feng Lan,Zhao Xiu Yun,Zhang De Shuang,Xu Jia Bing 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

The Design of Anti-aliasing Analog Filter for Data Acquisition in the Surface Measurement

Xu Jingbo,Xu Xiaohong,Yang Pengyu,Lin Haijun,Guo Xin 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

In the surface measurement system, the data acquisition is key part and the anti-aliasing analog filter is necessary. This paper deals with the design of the anti-aliasing analog filter. Based on the principle of anti-aliasing filtering, the parameters of filter are computed, the filter circuit is designed, and the frequency characteristic is drawn. Combined with digital filter, the filtering can maintain the low frequency components very well and suppress effectively the high frequency signals in the original surface profile, which reduces the distortion caused by noise and makes the filtering effect better. This method has been applied in the surface measurement system and the actually measured data verified the performance of the filter.

Enhanced Visible-Light Driven Photocatalytic Performances Over LaFeO3/NiO Modified Porous g-C3N4 Nanosheets

Ke Xu,Xiaosheng Yang,Dagao Sun,Xin Yang,Yumei Zhou,Wei Li,Qindan Yang,Xianjiong Yang,Rong Li,Jian Feng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.02

LaFeO3/NiO modified g-C3N4 nanosheets (L-N/CNS) were synthesized by a two-step method. HRTEM results showed that an intimate contact between LaFeO3, NiO and N-CNS was successfully established. Ninety percent of phenol was degraded within 120 min, and the hydrogen evolution rate of 171.2 μmol h -1 g -1 was obtained over the L-N/CNS heterojunctions under the visible-light irradiation. It was higher than that of g-C3N4 nanosheets, NiO modified g-C3N4 and LaFeO3/g-C3N4. The ·O2 - radicals acted the crucial role in the photocatalytic degradation reaction. EIS, PL and time-resolved fluorescence spectra demonstrated that L-N/CNS possessed the highest charge separation efficiency. The intimate contact between LaFeO3, NiO and g-C3N4 nanosheets promoted the separation and transfer of photo-induced electron–hole pairs and consequently prolonged the exciton lifetime, and implied more photo-induced electrons could be probably involved in the photocatalytic reactions on the surface of photocatalysts. Thus, the visible-light driven photocatalytic performances of L-N/CNS were effective. This work provided a feasible method to design and construct heterostructures for the exploitation of solar energy.

Investigation of the flow and force chain characteristics of metal powder in high-velocity compaction based on a discrete element method

Xu Zhi-Yang,Meng Fan-Jing 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.5

The flow and force chain characteristics of metal powder in high-velocity compaction are not fundamentally understood because of the complexities and discreteness of these particle systems. A 2D discrete element model of a metal powder subjected to uniaxial compaction is developed. The flow state of particles, the force chain distribution, and the influence of the main model parameters on the force chains are investigated. Results indicate a collisional particle flow stage at the beginning of compaction. Then, the flow state of particles can rapidly be classified as a dense particle flow. In addition, the contact force distribution for the collisional particle flow follows an exponential law, which is significantly different from that for dense particle flow. The probability distribution of contact forces changes as a power function in the dense particle flow. The force chains initially deviate in the y-axis direction and then in the x-axis direction with increasing friction coefficient, and the distribution and load-carrying rates of the weak force chains decrease with increasing friction coefficient. Moreover, the force chains deflect in the direction of the x-axis with increasing the particle diameter, and the distribution rate and load-carrying rate of the weak force chains decrease with increasing particle diameter. These changes in the direction of the force chains are similar to the density changes in the compacted blank part parts. The distribution rate and the load-carrying rate of the weak force chains decrease with increasing friction coefficient, and the density of the blank part depends on the direction of the entire force chains (vector sum of the force chains). Under the strength of the force chains with slight changes and when the entire force chains are inclined in the densification direction, the density of the compacted blank part increases.

Magnetic Properties of CoFe Films with in-plane Uniaxial Anisotropy Prepared by Electrodeposition

Yang Xu,Zhou Dong,Qiao Liang,Yang Yong,Li Fa-Shen 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Stability of generalized homogeneous cooperative systems

Yang Xu,Yuangong Sun 제어로봇시스템학회 2022 제어로봇시스템학회 국제학술대회 논문집 Vol.2022 No.11

In this paper, we study the asymptotic stability of nonlinear cooperative system by introducing a class of generalized homogeneous vector fields. With the help of the analytical techniques developed in homogeneous cooperative systems, we establish sufficient conditions for the global asymptotic stability of generalized homogeneous cooperative systems with time delays. When the involved system degenerates into a homogeneous cooperative system, sufficient and necessary conditions for the stability of the system are proposed. A simulation example is given to show the practicability of the conclusion.

A Mask Wearing Detection System Based on Deep Learning

Yang, Shilong,Xu, Huanhuan,Yang, Zi-Yuan,Wang, Changkun Korea Multimedia Society 2021 The journal of multimedia information system Vol.8 No.3

COVID-19 has dramatically changed people's daily life. Wearing masks is considered as a simple but effective way to defend the spread of the epidemic. Hence, a real-time and accurate mask wearing detection system is important. In this paper, a deep learning-based mask wearing detection system is developed to help people defend against the terrible epidemic. The system consists of three important functions, which are image detection, video detection and real-time detection. To keep a high detection rate, a deep learning-based method is adopted to detect masks. Unfortunately, according to the suddenness of the epidemic, the mask wearing dataset is scarce, so a mask wearing dataset is collected in this paper. Besides, to reduce the computational cost and runtime, a simple online and real-time tracking method is adopted to achieve video detection and monitoring. Furthermore, a function is implemented to call the camera to real-time achieve mask wearing detection. The sufficient results have shown that the developed system can perform well in the mask wearing detection task. The precision, recall, mAP and F1 can achieve 86.6%, 96.7%, 96.2% and 91.4%, respectively.