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( Woong Sic Jung ),( Sung Gu Lee ),( Se Won Kang ),( Yong Seok Lee ),( Jun Hyuck Lee ),( Sung Ho Kang ),( Eon Seon Jin ),( Hak Jun Kim ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.7
Expressed sequence tags (ESTs) from the Antarctic green algae Pyramimonas gelidicola were analyzed to obtain molecular information on cold acclimation of psychrophilic microorganisms. A total of 2,112 EST clones were sequenced, generating 222 contigs and 219 singletons, and 200 contigs and 391 singletons from control (4oC) and cold-shock conditions (-2oC), respectively. The complete EST sequences were deposited to the DDBJ EST database (http:// www.ddbj.nig.ac.jp/index-e.html) and the nucleotide sequences reported in this study are available in the DDBJ/EMBL/ GenBank. These EST databases of Antarctic green algae can be used in a wide range of studies on psychrophilic genes expressed by polar microorganisms.
Microsatellite DNA 를 이용한 연어(Oncorhynchus keta)와 곱사연어(O. gorbuscha)의 구분
정웅식,이윤호,신형철,성기백,김수암 한국수산자원학회 2001 한국수산자원학회지 Vol.4 No.-
본 연구에서는 형태적으로 구분이 불가능한 유통과정의 연어(Oncorhynchus keta)와 곱사연어(O. gorbuscha)를 구분하기 위하여 microsatellite DNA Ogo5와 Ogo6를 분석하여 비교하였다. 연어의 Ogo5 염기서열 반복구조는 네 가지 allele이 있었으며 곱사연어와 차이가 있었다: allele A, (GT)_2 GCATGC(GT)_2GCAC(GT)_3TCGGTTGCTTTT: allele B1, (GT)_2GTGTTT (GT)_2GCAT(GT)6TTTTTT; allele B2 (GT)_2 GTGTTT (GT)_2GCAT(GT)_7TTTTTT; allele B3 (GT)_2GTGTTT (GT)_2GCAT(GT)_7TTTTTTT: 곱사연어, (GT)_2GTGTTT (GT)_2GCAT(GT)_8TTTT. Ogo6 역시 연어와 곱사연어 사이에 반복구조의 길이에서 뚜렷한 차이를 보였다: 연어, (CA)_5AA(CA)_5CG와 CTCA(CT)_2GT; 곱사연어, (CA)_5AA(CA)_6AA(CA)_5CG와 CTCA(CT)_3GT. 또한, Ogo5와 Ogo6 염기서열은 두종 사이에 서로 다른 염기(single nucleotide polymorphism: SNP)가 각각 3개와 1개 있는 것으로 드러났다. 이러한 연구결과는 microsatellite DNA Ogo5와 Ogo6가 연어와 곱사연어를 형태적 구분이 불가능한 상태에서도 종 구분할 수 있는 유전자 마커로 사용될 수 있음을 시사한다. 이와 같은 유전학적 접근방법은 연어뿐만 아니라 시장에서 유통되는 다양한 수산물의 종과 원산지를 확인하는데 적용될 수 있다. Although the live chum salmon (Oncorhynchus keta) is discernable from the pink salmon (O. gorbuscha), processed salmon meat in the market place is hard to be distinguished from one species to the other. In order to resolve this situation, we analyzed microsatellite DNA Ogo5 and Ogo6 of the chum salmon and compared their sequences with those of the pink salmon. Four alleles of Ogo5 were identified in the chum salmon, which have distinct SNPs(Single Nucleotide Polymorphisms) and different numbers of repeat of (GT)n from the pink salmon: allele A. (GT)_2GCATGC(GT)_2GCAC(GT)_3TCGGTTGCTTTT: allele B1, (GT)_2GTGTTT(GT)_2GCAT(GT)_6TTTTTT: allele B2 (GT)_2GTGTTT(GT)_2GCAT(GT)_7TTTTTT: allele B3 (GT)_2GTGTTT(GT)_2GC AT(GT)_7TTTTTTT: the pink salmon, (GT)_2GTGTTT(GT)_2GCAT(GT)_8TTTT. Ogo6 of the chum salmon also has a distinct SNP and different numbers of repeats of (CT)n from that of the pink salmon: the chum salmon, (CA)_5AA(CA)_5CG and CTCA(CT)_2GT: the pink salmon, (CA)_5AA(CA)_6 AA(CA)_5CG and CTCA(CT)_3GT. These results suggest that the microsatellite DNA Ogo5 and Ogo6 would be good means of discrimination for the salmon meat in the market. Use of genetic markers as shown in this study could be applied to many different kinds of fish products for the identification of the species and their origins.
남극 호냉성 미세조 Porosira pseudodenticulata의 형태와 분자적 자료
정웅식,주형민,홍성수,강재신,최한구,강성호,Jung, Woong-Sic,Joo, Hyoung-Min,Hong, Sung-Soo,Kang, Jae-Shin,Choi, Han-Gu,Kang, Sung-Ho 한국조류학회(藻類) 2006 ALGAE Vol.21 No.2
We have cultured more than 100 Arctic and Antarctic cryophilic microalgal strains in KOPRI culture collections of polar microorganisms (KCCPM). Among them, we tried to identify an Antarctic strain, KOPRI AnM0008 by morphological and molecular analysis. Nuclear SSU rDNA and plastid rbcL sequences were used to identify the strain. It was identified as Porosira pseudodenticulata based on SSU sequence data showing 99% identity with GenBank X85398. This result was supported by morphological features like solitary labiate process, external foramina and internal cribra by optical and scanning electron microscope. Morphological identification and molecular analysis on polar cryophilic microalgae will be accomplished to construct the databases for KCCPM.