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Soo-Kwon Park,Woon-Ha Hwang,Dong-Jin Shin,Yeon-Jae Hur,Tae-Heon Kim,Sang-Ik Han,Jun-Hyun Cho,Jong-Hee Lee,Ji-Yoon Lee,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Green rice leafhopper(GRH), Nephotettix cincticeps Uhier, is one of the major insect pests of rice (Oryza sativa L.) in the temperate growing region of East Asia. GRH sucks sap from both xylem and phoem of susceptible rice varieties, and increased GRH populations cause sooty mold disease on the ears of rice after heading stage. In addition to direct plant destruction, GRH also causes damage to rice plants by transmitting rice dwarf viruses causing rice dwarf viruses disease which could decrease the yield of rice. Development of GRH resistant rice varieties for reducing yield loss is an important objective in current breeding programs. In this study, we developed three SSR markers(RM18166, RM516, RM18171) and one Indel marker(Indel15040) which could select Grh1-resistant varieties using population derived from cross lines between Grh1-resistant variety ‘Singwang’ which contains Grh1 gene and susceptible variety ‘Ilpum’. PCR products of RM18166 which was one of the developed markers were easily detected in agarose gel. These markers will be useful for development of the Grh1-resistant varieties through marker-assisted selection(MAS) without bio-examination in rice breeding
QTL analysis of the qBK1, a major QTL for bakanae disease resistance in rice
Yeon-Jae Hur,Saes-Beul Lee,Tae-Heon Kim,Jong-Hee Lee,Dong-Jin Shin,Soo-Kwon Park,Woon-Ha Hwang,Sang-Ik Han,Jun-Hyun Cho,Young-Nam Yoon,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Bakanae disease is one of the most serious and oldest problems of rice production, which was first described in 1828 in Japan (Ito and Kimura 1931). This disease may infect rice plants from the pre-emergence stage to the mature stage, with severe infection of rice seeds resulting poor germination or withering (Iqbal et al. 2011). Under favorable environmental conditions, infected plants have the capacity to produce numerous conidia that subsequently infect proximate healthy plants, resulting in major yield loss (Ou 1985). One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The results of this study are expected to provide useful information toward developing resistant rice lines to this detrimental fungal disease.
Bone regenerative effect of recombinant human bone morphogenetic protein-2 after cyst enucleation
Hwang, Doo Yeon,On, Sung Woon,Song, Seung II Korean Association of Maxillofacial Plastic and Re 2016 Maxillofacial Plastic Reconstructive Surgery Vol.38 No.-
Background: The aim of this study is to quantitatively evaluate the effect of rhBMP-2 for repair of bone defects after cyst enucleation using the osteogenesis index (OI). Methods: Under general anesthesia, 10 patients (12 lesions) underwent oral or maxillofacial surgery for cyst enucleation. Postoperatively, 12 lesions were divided into two groups: group A (six lesions) was treated with absorbable collagen sponge (ACS) in combination with rhBMP-2, and group B (six lesions) was treated with ACS alone. After 3 months, cone-beam computed tomographic scans were obtained to measure changes in the volume of the lesions. We then calculated the OI of each group at two different Hounsfield units to determine any statistically significant difference between these two groups (Mann-Whitney U test). Results: As tested at the level of new bone, the mean OI was 72.37 % in group A and 55.08 % in group B -a statistically significant difference (p = 0.041). As tested at the level of mature bone, the mean OI was 27.47 % in group A and 18.88 % in group B, but the difference was not statistically significant (p = 0.394). Conclusions: The application of rhBMP-2 after maxillofacial cyst enucleation accelerated new bone formation in the bone defects. Thus, the use of rhBMP-2 in combination with ACS may be considered an alternative to conventional bone grafting in some patients with postoperative bone defects.
Identification of a major quantitative trait locus for bakanae disease resistance in rice
Yeon-Jae Hur,Saes-Beul Lee,Tae-Heon Kim,Jong-Hee Lee,Dong-Jin Shin,Soo-Kwon Park,Woon-Ha Hwang,Sang-Ik Han,Jun-Hyun Cho,Young-Nam Yoon,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Bakanae disease incidence threat is an increasing trend in the top rice growing countries. Despite it is essential to identify the resistant genes and underlying mechanisms of bakanae disease to develop resistant varieties, there are very limited genetic studies on bakanae disease in rice. The indica rice variety Shingwang was selected as resistant donor to bakanae disease. One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). The proportion of healthy plants of Shingwang and Ilpum after inoculation was confirmed using bakanae disease pathogen, CF283. While inoculated Ilpum showed thin and yellowish-green phenotype which is typical symptom of Bakanae disease, Shingwang showed similar healthy phenotype with control plants. A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The information of qBK1 could be useful for improving rice bakanae disease resistance in marker-assisted breeding.
Sump 증후군에 의한 간농양의 간위루를 통한 자발성 농양배액 1예
황재진,하창윤,장현정,윤은영,주지현,안연정,민현주,김태효,김현진,정운태,이옥재,이선영 이화여자대학교 의과학연구소 2010 EMJ (Ewha medical journal) Vol.33 No.2
A 55-year-old man was admitted to our hospital with symptom of fever, chilling, abdominal discomfort and weight loss for 2months. Abdominal computed tomography(CT) revealed a 5×3.75 cm sized low attenuated lesion in the left lateral segment of liver. Esophagogastrodedodenoscopy showed a fistula with dirty exudates at the fundus and a yellowish stone and food debris at the choledochoduodenostomy site. Endoscopic retrograde cholangiopancreatography (ERCP) was performed and stone and food materials in common bile duct was removed with snare and basket. We experienced a case of liver abscess due to sump syndrome and spontaneous drainage to the stomach.
혼합용매법에 의한 Mullite 전미분체 생성기구에 관한 연구
황운연,이정운,박형상,김용렬,구기갑 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.4
Si-와 Al-알콕시드의 반응속도 차이를 조절하기 위한 부분 가수분해법과 입자 형태를 제조하기 위한 혼합용매법을 이용하여 구형의 미세한 mullite 전미분체를 제조하였다. Mie 이론을 적용시켜 반응시간에 따른 반응용액의 탁도 및 입자 수밀도의 변화를 측정하여 혼합용매법에 의한 입자 생성기구에 대하여 고찰하였다. 알콕시드와 옥탄올 용액에 아세토니트릴을 첨가하면 알콕시드의 용해도가 낮아지고 따라서 알콕시드의 일부가 액적을 형성하며, 액적은 안정화제 (HPC)에 의하여 구형을 유지하게 된다. 그리고 가수분해 및 축합중합 반응에 의하여 액적의 입자 크기는 감소하며, 동시에 혼합용액에 용해되어 있던 알콕시의 가수 분해 및 축합중합 반응에 의하여 미세 입자가 생성된다. 액적형태로 생성된 큰 입자와 미세 입자의 응집에 의하여 최종 입자가 생성되었다. In this study, spherical fine pre-mullite particles were prepared by partial hydrolysis method to control the reaction rate difference of two alkoxides, and by mixed solvent method to control the particle shape. Based on the adaptation of Mie theory, the formation mechanism of pre-mullite particle in alkoxide-octanol-acetonitile system has been investigated by measuring of the turbidity of solution, the number density and the size of the particles during the reaction. As the acetonitrile was added in the alkoxide-octanol solution, the solubility of the alkoxide decreased. A part of alkoxide was segregated from solution in the form of droplets. whereas the rest of the alkoxide existed as dissolved state in octanol. The droplets are stabilized with stabilizer (HPC). In the next step. hydrolysis and condensation reactions of the alkoxide droplets decrease the size of droplets and, at the same time, fine particles are formed from the alkoxide dissolved in octanol. Subsequently, the final particles are produced by aggregation between the large particles through the droplet form and the fine particles.
뇌졸중 후 상지기능 개선을 위한 스마트폰 어플리케이션 개발
김황용(Hwang-Yong Kim),허명(Myoung Heo),김다운(Da-Woon Kim),김정환(Jeong-Hwan Kim),곽연상(Yeon-Sang Kwak),손재광(Jae-Kwang Son),장환석(Hwan-Seok Jang),최경현(Kyeong-Hyeon Choi),이제성(Je-Seong Lee) 한국엔터테인먼트산업학회 2015 한국엔터테인먼트산업학회논문지 Vol.9 No.2
스마트폰 무선인터넷 사용량이 늘어나면서 스마트폰 어플리케이션에 대한 수요와 공급이 급속히 증가한 반면에 뇌졸중 재활분야에서의 어플리케이션은 국내에 거의 미비한 실정이다. 그러므로 본 연구는 뇌졸중 상지기능 개선에 효과가 검증된 단계별 반복적 팔 보조 프로그램(Graded Repetitive Arm Supplementary Program ; GRASP)을 한국 실정에 맞게 수정 보완 하여 뇌졸중 환자들의 본인 상태에 맞게 3단계로 구성하여 동영상으로 제작한 후 스마트폰 어플리케이션을 개발하여 구글 플레이에 등록하였다. 본 연구에서 개발한 모바일 어플리케이션을 통해 병원에서는 재활치료 후 남은 시간에 자가 운동 할 수 있도록 가이드를 제공할 수 있고, 퇴원 후 가정에서는 뇌졸중 환자들이 쉽게 따라 운동 할 수 있는 자조관리 재활훈련 서비스를 제공할 수 있어서 뇌졸중 후 상지기능 회복에 도움을 줄 것으로 기대한다. Smartphone application programs for stroke rehabilitation are few, if any, in Korea while wireless internet access is getting much easier and general smartphone application programs are rapidly increasing. The purpose of this study was to develop a smartphone application program for upper extremity rehabilitation of individuals with stroke, and to register it in the Google play. This study used the Graded Repetitive Arm Supplementary Program (GRASP) as the basic rehabilitation program and modified it for the Korean culture. GRASP has been proven to be effective on improving the function of upper extremity of the individuals with stroke. The smartphone application program of this study consists of three levels of video segments which were made by the researchers for the study. The application program is expected to provide stroke patients with an useful guide to exercise by themselves at home or in hospitals.