디지털 워터마킹을 이용한 영상 정보 보호 시스템

박정빈,정성환,이우선 國立 昌原大學校 精報通信硏究所 1999 精報通信論文集 Vol.3 No.-

Recently, with advance in computer network and multimedia technology, digital media is rapidly proliferating, and digital copy has been widely practiced. So an efficient technology for the protection of intellectual property is required. Watermark techniques effectively protect the intellectual property for multimedia data, and can prevent an unauthorized person from copying the multimedia data. In this paper, we propose a watermarking scheme to hide watermark information in low-frequency and high-frequency of wavelet transform. Experimental results show that the proposed watermark scheme is robust to several distortions including Gaussian noise and filtering, compression and contrast at different qualities.

에이즈 환자의 거대세포바이러스 감염증

김홍빈,박상원,김남중,최희정,신동현,오명돈,김우호,정흠,최강원 대한감염학회 1998 감염 Vol.30 No.4

배 경 : 거대세포바이러스 감염은 인간면역부전바이러스 (Human Immunodeficiency Virus, HIV) 감염자에서 발생하는 가장 중요한 기회감염증의 하나이다. 특히, 국내에서는 95%이상의 국민이 거대세포바이러스의 1차감염을 경험한 상태이므로 에이즈 환자에서 거대세포바이러스 질환의 빈도가 높을 것으로 예상된다. 대상 및 방법 : 1987년 10월부터 1996년 9월까지 서울대학교 병원에서 추적 관찰하였던 128명의 HIV 감염자를 대상으로 하였다. 이들 환자의 의무 기록에서 임상 자료를 얻었다. 거대세포바이러스 망막염은 안과 전문의의 임상적인 소견으로 진단하였으며, 위장관 등 망막이외 부위의 거대세포마이러스 질환은 조직학적으로 확인된 경우에 한하였다. 결 과 : 대상환자 128명의 추적관찰기간은 중앙값이 6(0∼59)개월 이었으며 7명 (5.4%)에서 거대세포바이러스 질환이 발생하였다. 거대세포바이러스 질환은 망막염이 6예, 식도염이 2예, 대장염이 1예, 폐렴 및 부신감염이 1예, 범발성 감염이 1예였다. 거대세포바이러스 질환의 발생빈도는 CD4+ 림프구 수가 적을수록 높았으며, CD4+ 수가 200/㎣미만인 환자 중 15% (6/33)에서 거대세포바이러스 질환이 확인되었다. 11예 중 9예를 ganciclovir로 치료하였으며 이중 7예에서 호전 또는 진행의 억제가 확인되었다. 추적관찰이 가능한 6예 중 3예 (50%)에서 재발하였다. 결 론 : 국내 에이즈 환자에서 거대세포바이러스 질환은 비교적 흔한 기회감염증이다. Background : Cytomegalovirus (CMV) infection is one of the important opportunistic infections in immunocompromised patients. In Korea, seroprevalence of IgG against CMV is over 95%. Therefore, CMV diseases are expected to be a prevalent opportunistic infection in AIDS patients. in Korea. Methods : We reviewed the medical records of 128 patients with HIV infection who visited the Seoul National University Hospital during the period from Nov. 1987 TO Sep. 1996. All the patients were examined by one ophthalmologist and the diagnosis of CMV retinitis were made by funduscopic findings. Other CMV diseases were diagnosed when histopathologic examinations showed the characteristic cytomegalic cells. Results : Median duratior, of follow-up was 8 months. Eleven CMV diseases were found in 7 patients (5.4%): 6 patients ahd retinitis, 2 esophagitis, 1 colitis, 1 pneumonitis, and one patient developed disseminated infection. Fifteen percent (6/33) of the patients whose CD4+ lymphocyte counts were less than 200/㎣ at baseline developed CMV diseases. Out of the 9 cases treated with ganciclovir, 7 improved or were stabilized. No patient received maintenance treatment and 3 had relapsed. Conclusion : CMV diseases are common opportunistic infections in AIDS patients in Korea.

탈감작법으로 치료한 인형 재조합 인슐린에 대한 아나필락시스

박경우(Kyung Woo Park),박현신(Hyun Shin Park),박우상(Sang Woo Park),장철순(Chul Soon Jang),정정환(Chung Whan Chung),홍성빈(Seong Bin Hong),김용성(Yong Seong Kim),박원(Won Park),송정수(Jung Soo Song),최승원(Seung Won Choi) 대한내과학회 2002 대한내과학회지 Vol.62 No.2

N/A Allergic reaction to insulin is mediated by several mechanisms; differences in amino acid sequence of animal and human insulin, altered tertiary structure of insulin and the presence of non-insulin protein contaminants or pharmaceutical additives. Anaphylactic reactions to insulin only occur in 0.1 to 2% of patients who stopped insulin therapy and have then resumed treatment. We report a diabetic patient who suffered severe anaphylactic reactions to human recombinant insulin, successfully treated by desensitization. A 19-year-old man with type 1 diabetes receiving Insulatard HM? developed generalized urticaria and angioedema with progression to dyspnea, dizziness and syncope. Skin prick test to all kinds of human recombinant insulin products revealed immediate type hypersensitivity and the titer of insulin IgE was increased in serum. The desensitization trial with Velosulin HM? using modified desensitization method was performed. Six months after the desensitization he was taking Velosulin HM? as well as Insulatard HM? without any evidence of systemic allergic reactions.(Korean J Med 62:204-208, 2002)

Germination Characteristics and Seed Dormancy of Iris dichotoma Pall., an Endangered Species Native to Korea

Park, Hyeong Bin,Lee, Byoung-Doo,Lee, Chang Woo,Hwang, Jung Eun,Park, Hwan Joon,Kim, Seongjun,An, Jiae,Kim, Pyoung Beom,Kim, Nam Young National Institute of Ecology 2021 국립생태원회보(PNIE) Vol.2 No.4

Iris dichotoma Pall. found on Daechung Island in Korea has been designated as an endangered species. To aid in conservation efforts of this species, this study investigated its germination characteristics and seed dormancy type. Four sets of seeds were incubated at different temperatures (4/1℃, 15/6℃, 20/10℃, and 25/15℃). One set of seeds was cold stratified (4 weeks at 4/1℃). The final germination rate and mean germination time showed that the optimal germination temperature was 25/15℃. Final germination rates were ~70%, showing no significant difference among temperature treatments. However, mean germination time were significantly different among all temperature treatments except for 4/1℃. Mean germination time for seeds with temperature treatments of 15/6℃, 20/10℃, and 25/15℃ were 3.2, 2.1, and 1.5 weeks, respectively. At 25/15℃, the mean germination time was half of that at 15/6℃. Seeds of I. dichotoma had fully developed embryos at the time of dispersal. No additional growth of the embryo was observed. Cold stratification did not affect the final germination rate or the mean germination time. This study shows that seeds of I. dichotoma have no physiological or morphological dormancy, unlike other members of the Iris genus known to have seed dormancy that needs a relatively high incubation temperature (≥25/15℃) for mass propagation to occur. These results will be useful for understanding ecophysiological mechanisms related to the species' habitat. They are also useful for mass propagation of I. dichotoma for the purpose of conserving this endangered species.

Long-Term Clinical Outcomes of True and Non-True Bifurcation Lesions According to Medina Classification- Results From the COBIS (COronary BIfurcation Stent) II Registry.

Park, Taek Kyu,Park, Yong Hwan,Song, Young Bin,Oh, Ju Hyeon,Chun, Woo Jung,Kang, Gu Hyun,Jang, Woo Jin,Hahn, Joo-Yong,Yang, Jeong Hoon,Choi, Seung-Hyuk,Choi, Jin-Ho,Lee, Sang Hoon,Jeong, Myung-Ho,Kim, Japanese Circulation Society 2015 Circulation journal Vol.79 No.9

<P>Little is known about the clinical outcomes of patients with different types of coronary bifurcation lesions. We sought to compare long-term clinical outcomes of patients with true or non-true bifurcation lesions who underwent percutaneous coronary intervention.Methods?and?Results:We compared major adverse cardiac events (MACE: cardiac death, myocardial infarction [MI], or target lesion revascularization) between 1,502 patients with true bifurcation lesions (51.8%) and 1,395 with non-true bifurcation lesions (48.2%). True bifurcation lesions were defined as Medina classification (1.1.1), (1.0.1), or (0.1.1) lesions. During a median follow-up of 36 months, MACE occurred in 296 (10.2%) patients. Patients with true bifurcation lesions had a significantly higher risk of MACE than those with non-true bifurcation lesions (HR 1.39; 95% CI 1.08-1.80; P=0.01). Among true bifurcation lesions, Medina (1.1.1) and (0.1.1) were associated with a higher risk of cardiac death or MI than Medina (1.0.1) (HR 4.15; 95% CI 1.01-17.1; P=0.05). During the procedure, side branch occlusion occurred more frequently in Medina (1.1.1) and (1.0.1) than Medina (0.1.1) lesions (11.5% vs. 7.4%, P=0.03).</P>

Analysis of Molecular Genomics of Brucella abortus Mutants Generated by Ez-Tn5TM pMODTM-3 Transposon

Woo Bin Park,Young Bin Im,Myunghwan Jung,Han Sang Yoo 대한수의학회 2015 대한수의학회 학술대회발표집 Vol.2015 No.-

Impact of Electric Vehicle Penetration-Based Charging Demand on Load Profile

Woo-Jae Park,Kyung-Bin Song,Jung-Wook Park 대한전기학회 2013 Journal of Electrical Engineering & Technology Vol.8 No.2

This paper presents a study the change of the load profile on the power system by the charging impact of electric vehicles (EVs) in 2020. The impact of charging EVs on the load demand is determined not only by the number of EVs in usage pattern, but also by the number of EVs being charged at once. The charging load is determined on an hourly basis using the number of the EVs based on different scenarios considering battery size, model, the use of vehicles, charging at home or work, and the method of charging, which is either fast or slow. Focusing on the impact of future load profile in Korea with EVs reaching up 10 and 20 percentage, increased power demand by EVs charging is analyzed. Also, this paper analyzes the impact of a time-of-use (TOU) tariff system on the charging of EVs in Korea. The results demonstrate how the penetration of EVs increases the load profile and decreases charging demand by TOU tariff system on the future power system.

Analysis of protein expression in Brucella abortus mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis

Woo Bin Park,Young Bin Im,Soojin Shim,유한상 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.2

Brucella abortus is a bacterium that causes brucellosis and is the causative agent of worldwide zoonoses. Pathogenesis of the B. abortusinfection is complicated, and several researchers have attempted to elucidate the infection mechanism of B. abortus. While several proteinshave been revealed as pathogenic factors by previous researchers, the underlying mechanism of B. abortus infection is unresolved. In thisstudy, we identified proteins showing different expression levels in B. abortus mutants with different biological characteristics that weregenerated by random insertion of a transposon. Five mutants were selected based on biological characteristics, in particular, their growthfeatures. Total proteins of mutant and wild-type B. abortus were purified and subjected to two-dimensional gel electrophoresis. Thirty proteinspots of each mutant with expression increases or decreases were selected; those with a change of more than 2-fold were compared with thewild-type. Selected spots underwent liquid chromatography tandem mass spectrometry for peptide analysis. DnaK and ClpB, involved inprotein aggregation, increased. SecA and GAPDH, associated with energy metabolism, decreased in some mutants with a growth rate slowerthan that of the wild-type. Mutants with slower growth showed a decrease in energy metabolism-related proteins, while mutants with fastergrowth showed an increase in pathogenicity-related proteins.

Molecular characteristics of Brucella abortus mutants generated using EZ-Tn5Tm pMODTm-3 transposon system

( Woo Bin Park ),( Young Bin Im ),( Myunghwan Jung ),( Han Sang Yoo ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.4

Brucella abortus is a well-known intracellular zoonotic pathogen. Despite significant research to understand the pathogenesis, underlying mechanisms of the bacterial infection are not yet understood. Thus, prevention and control of the B. abortus infection is problematic in animal and human. Therefore, several methods involving random mutation have been used to identify the mechanisms and provide a solution for control and prevention of Brucella infection. B. abortus mutants were generated by random insertion of a transposon, Ez-Tn5TM pMODTM-3 <R6Kγori/MCS> into a chromosome. Characteristics of these mutants were investigated using biochemical testing, growth features, determination of biovar, antibiotic resistance and detection of virulence factors, T4SS, PGK, and CGS. In biochemical testing, B. abortus mutants were categorized according to 7 groups with different condition of ILATk, SUTC, and ELLM. Different growth features were also observed between wild type and mutants. In addition, both B. abortus wild type and mutants were determined as biovar type 1 by biovar test. Three virulence factors, T4SS, PGK, and CGS were detected by PCR. Therefore, B. abortus mutants were characterized by analysis of phenotyping and it might be useful for further studies of known pathogenesis of B. abortus infection and for identification of diagnostic markers of brucellosis.

Analysis of Protein Expressions in Brucella abortus Mutants by Two Dimensional Gel Electrophoresis and LC-MS/MS Peptide Analysis

( Woo Bin Park ),( Young Bin Im ),( Soojin Shim ),( Han Sang Yoo ) 대한인수공통전염병학회 2017 창립총회 및 학술대회 초록집 Vol.2017 No.1

Introduction: Brucella abortus is a bacterium that causes brucellosis and is the causative agent of zoonotic disease that infect both animals and human. Pathogenesis of the B. abortus. infection is so complicated. Thus, several investigations have been made to solve the mechanism in infection of B. abortus. While several proteins are suggested as pathogenic factors through the researches, underlying mechanism of the B. abortus infection is still left to be resolved. Objective of this study is to identify the proteins showing the different expression level in B. abrotus mutants with different biological characteristics. Methods: B. abortus mutants were generated by random insertion of transposon, EZ-Tn5^TM. Of the mutants, five mutants, C3, C8, C13, C24, and C30 were selected based on the growth features. Total proteins of 5 mutants and wild type of B. abortus were purified and subjected to two dimensional gel electrophoresis. 30 spots of protein were selected each with an increase and decrease, more than 2-fold as much change was confirmed as compared with B.abortus wild type. And selected spots were analyzed using LC-MS/MS and Mascot. Results: Two dimensional gel electrophoresis analysis showed 814 spots in B. abortus wild type. A total of 541 spots were showed in mutant C3, 577 in mutant C8, 491 in mutant C13, 516 in mutant C24, and 560 in mutant C30. Among them, 444 spot in mutant C3, 418 in mutant C8, 256 in mutant C13, 246 in mutant C24, and 282 in mutant C30 were the pairs forming B. abortus wild type pairs. Protein analysis was selected in 30 spots, which were commonly increased or decreased in mutants. All spots were detected on 2-DE gels within pI and molecular weight ranges of 4.0-10.0 and 10-250kDa. ClpB and DnaK involved in protein aggregation were simultaneously increased. The bichaperone network of DnaK and ClpB consists of three stages. When DnaJ is attached, the cochaperone forms an aggregation with DnaK, DnaK interacts with ClpB on the surface on the aggregation. DnaK, as a heat shock protein, is known to be a critical factor in the intracellular infection of the B. abortus. Expression of ClpB was increased in mutants C13, C24, and C30. And DnaK increased expression level in all mutants. Proteins associated with energy metabolism, SecA and GAPDH were reduced in the expression level in mutants C3 and C8 and all mutants, respectively. OMP16 known as a major virulence factor of B. abortus were increased in mutants C13, C24, and C30. Factors related to the growth, such as RplK were increased in the expression in mutants C3, C8, C24, and C30. Conclusions: Mutants, C3 and C8, with slower growth showed decrease with energy metabolism related proteins, such as SecA and GAPDH, while mutants C13, C24 and C30 with faster growth showed increase in the pathogenicity related proteins such as ClpB, DnaK, and OMP16. Acknowledgment: This work was supported by KHIDI (No. HI16C2130), BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.