Tamlana agarivorans sp. nov., isolated from seawater off Jeju Island in Korea

Yoon, J.-H.,Kang, S.-J.,Lee, M.-H.,Oh, T.-K. Microbiology Society 2008 International journal of systematic and evolutiona Vol.58 No.8

<P>A Gram-negative, non-motile, rod-shaped, agarolytic bacterial strain, designated JW-26T, was isolated from seawater off Jeju Island in Korea, and was subjected to a polyphasic taxonomic investigation. Strain JW-26T grew optimally at pH 7.0-8.0, at 30 degrees C and in the presence of approximately 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain JW-26T joins Tamlana crocina HST1-43T, with which it exhibited a 16S rRNA gene sequence similarity value of 95.5 %. Strain JW-26T contained MK-6 as the predominant menaquinone and iso-C15:0, C15:0 and iso-C15:1 as the major fatty acids. The DNA G+C content was 36.8 mol%. There were no distinct phenotypic and, particularly, chemotaxonomic properties to differentiate strain JW-26T from the genus Tamlana. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain JW-26T is distinguishable from the type strain of T. crocina. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain JW-26T represents a novel species within the genus Tamlana, for which the name Tamlana agarivorans sp. nov. is proposed. The type strain is JW-26T (=KCTC 22176T =CCUG 55327T).</P>

정상 임신에서 임신주기에 따른 갑상선기능의 변화

김원배,정재훈,윤보현,이석인,김민선,오태근,조보연,이홍규,고창순 대한내분비학회 1994 Endocrinology and metabolism Vol.9 No.3

It is well known that normal pregnancy is accompanied by a rise in serum concentrations of thyroxine-binding globulin(TBG) and human chorionic gonadotropin (hCG). Alterations of biochemical parameters of thyroid function are recognized during gestation and sensitive tests to evaluate the alterations easily are required. Therefore, a cross-sectional study was undertaken in 140 healthy pregnant women to evaluate the efficacy of free T_4 measured by 2-step RIA compared to other thyroid function tests and to confirm the changes of thyroid function according to the stages of normal pregnancy. The sensitivities of free T_4 index, free T_4(by 2-step RIA), T_3 and TSH were realtively high(99.3%, 93.6%, 92.9%, 83.6%, respectively) compared to those of T_4 and T_3 bead upgake(49.3%, 21.4%) during all stages of pregnancy. There were positive correlations between free T_4 index and free T_4 or total T_4(r=0.68, r=0.72; p$lt;0.001). The values of free T_4 index sharply decreased from 3.22+-0.10(meam +-SEM) during 6th-12th week to an plateau after 16th-20th week of gestation(p$lt;0.01). The serum concentrations free T_4 and T_3 bead uptake also significantly decreased from 1.65+-0.05 ng/dl, 24.7+- 0.7% during 6th-12th week to an plateau after 16th-20th week of gestation, respectively(p$lt;0.001), No differences were found in the changes of serum concentrations of T_3, T_4 and TSH according to the stages of pregnancy. In conclusion, it is adequate to measure some tests including free T_4 index and free T_4 to evaluate thyroid function during pregnancy. The thyroid physiology and changes of thyroid function according to the stages of pregnancy should be considered in the interpretation of thyroid function status during pregnancy(J Kor Soc Endocrinol 9: 183-189, 1994).

Search for theH-dibaryon resonance inC12(K−,K+ΛΛX)

Yoon, C. J.,Akikawa, H.,Aoki, K.,Fukao, Y.,Funahashi, H.,Hayata, M.,Imai, K.,Miwa, K.,Okada, H.,Saito, N.,Sato, H. D.,Shoji, K.,Takahashi, H.,Taketani, K.,Asai, J.,Kurosawa, M.,Ieiri, M.,Hayakawa, T. American Physical Society 2007 PHYSICAL REVIEW C - Vol.75 No.2

자돈 설사병 예방백신의 개발

윤여창,김태종,이중복,이기수,윤화중,황도윤 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

Exteroxigenic Escherichia coli(ETEC) is a major cause of diarrhea in neonatal and weaning piglets. The incidence of ETEC in piglets with diarrhea was investigated by detecting the K88, K99 and 987P antigens from the isolated strains of Escherichia coli(E.coli). This study was carried out to investigate the biochemical characteristic, antibiotic susceptibililty and development of E.coli vaccine against diarrheal disease in piglets. The results obtained from this study were summarized as follows: 1. 24 strains of K88 positive ETEC were isolated from 64 E.coli. 2. In antibiotic susceptibility test, K88 positive ETEC showed high sensitivity to imipenem but resistance to penicillin and vancomycin. 3. 16 strains were positive for heat labile toxin, but not shown hemolysis. 4. The purified pilus of K88 positive ETEC had molecular weight of 28.5 KDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 5. Serum antibody responses to immunogen in guinea pigs given experimental oil and gel emulsion vaccine peaked at 4 and 6 week after vaccination. 6. In the mean hemagglutination inhibition titer to antigens of guinea pigs, oil emulsion pili vaccine was 1:1280 and those given gel emulsion pili vaccine was 1:640 respectively. 7. Agglutination titers in pregnant gilts given the oil emulsion pili vaccine were significantly higher compared to those of control groups. 8. The protective efficacy of the oil emulsion pili vaccine was 77.3% under field condition.

Friction Behavior of High Velocity Oxygen Fuel (HVOF) Thermal Spray Coating Layer of Nano WC-Co Powder

T.Y. Cho,J.H. Yoon,K.S. Kim,W. Fang,Y.K. Joo,K.O. Song,S.J. Youn,S.Y. Hwang,H.G. Chun 한국표면공학회 2007 한국표면공학회지 Vol.40 No.4

High Velocity Oxygen Fuel (HVOF) thermal spray coating of nano size WC-Co powder (nWC-Co) has been studied as one of the most promising candidate for the possible replacement of the traditional hard plating in some area which causes environmental and health problems. nWC-Co powder was coated on Inconel 718 substrates by HVOF technique. The optimal coating process obtained from the best surface properties such as hardness and porosity is the process of oxygen flow rate (FR) 38 FMR, hydrogen FR 57 FMR and feed rate 35 g/min at spray distance 6 inch for both surface temperature 25℃ and 500℃. In coating process a small portion of hard WC decomposes to less hard W₂C, Wand C at the temperature higher than its decomposition temperature 1,250°C resulting in hardness decrease and porosity increase. Friction coefficient increases with increasing coating surface temperature from 0.55-0.64 at 25℃ to 0.65-0.76 at 500℃ due to the increase of adhesion between coating and counter sliding surface. Hardness of nWC-Co is higher or comparable to those of other hard coatings, such as A1₂O₃, Cr, Cr₂O₃ and HVOF Tribaloy 400 (T400). This shows that nWC-Co is recommendable for durability improvement coating on machine components such as high speed spindle.

Comparison of HVOF Thermal Spray Coatings of T800 and WC-Co Powders

T. Y. Cho,J. H. Yoon,K. S. Kim,N. K. Baek,K. O. Song,S. J. Youn,S. Y. Hwang,H. G. Chun 한국표면공학회 2006 한국표면공학회지 Vol.39 No.6

Hard chrome plating has been used in surface hard coating over 50 years both for applying hard coating and re-building of worn components. Hard chrome plating solution and mist pollute environment with very toxic Cr??(hex-Cr) known as carcinogen which causes lung cancer. High velocity oxy-fuel (HVOF) thermal spray coatings of WC base cermet and Co-alloy powders are the most promising candidates for the replacement of the traditional hard chrome plating. Surface properties, wear, and friction behaviors of micron size Co-alloy (T800) and micron size WC-12Co (WC-Co) have been studied for the application as hard coatings. The temperature dependence of wear and friction behaviors of T800 and WC-Co have been investigated at the temperature of 25℃ and 538℃ for the application to high speed spindle.

소 성장호르몬 유전자의 Exon 5번에서의 새로운 다형성 연구

윤두학,김태헌,이경희,박응우,이학교,정일정,홍기창 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.1

성장호르몬 유전자는 하나의 작은 공통 선조 유전자로부터 아주 오랜 기간동안 유전자 중복에 의해 진화되어 온 그룹들 중의 하나이다. 이들에 속하는 유전자들은 동물 종간에 구조적인 상동성과 기능적 공통성 등 유사성이 비교적 높게 나타난다. 이런 연구결과들을 근거로 하여 소 성장호르몬 유전자에서 아미노산을 암호화하는 영역으로부터 새로운 아미노산의 변이(missense mutation)를 검출하였고, 이 변이의 대립유전자 빈도는 소(cattle)의 종(species) 및 품종의 지리적 분포에 따라 일정한 경향 치를 보여 주었다. 한편 변경되어진 아미노산은 Tryptophan으로 이는 생물체에 존재하는 많은 단백질들을 구성하는 아미노산중에서도 그 출현빈도가 가장 낮은 것이다. 또한 검출된 변이는 성장호르몬이 그의 수용체와 강하게 결합하는 부위로서, 성장호르몬의 구조적 변이를 초래하여 수용체와의 결합이 비정상적으로 이루어져, 이후 성장호르몬이 표적세포로의 신호전달과 같은 역할을 제대로 수행치 못하게 되고, 이로 인하여 가축의 표현되어지는 경제형질에 영향을 미칠 것으로 추정된다. 그러므로 이러한 대립유전자를 보유하는 개체는 집단에서 제거하는 방법에 의한 개량이 가능할 것으로 사료된다. Growth Hormone (GH) gene is a member of gene family through the evolutionary process from a small common ancestral gene by a series of gene duplications. The role of the GH in growth and performance controls has bees extensively studied in human, mice and livestock. Many researchers have considered GH as a strong candidate gene for evaluation of genetic polymorphisms that could be associated with economic traits in cattle. We report here a novel missense mutation within the exon 5 of the bovine Growth Hormone (bGH) gene. We could amplified 522 bp fragments from eight unrelated Hanwoo cattle by PCR, the, subsequently cloned and sequenced. An Msp I RFLP corresponding to a C to T transition was observed at position 2258 nt. From this result, we could predict a missense mutation (Arg to Trp) at codon 166 in a highly conserved region among many mammals. Codominant Mendelian segregation of the two alleles, Msp I (+) and Msp I (-), was observed in two full-sib F2 families (n = 32, African taurine Bos taurus × African zebu Bos indicus) and eight half-sib Hanwoo families. For the availability of genetic marker, we have performed PCR-RFLP with a large number of individual animals from 15 different cattle breeds (European and Asian taurines. and African indicines). Consideration of breed frequencies of Msp I (=) allele in relation to breed type and their geographic origins, shows higher frequencies in humped breeds or Asian cattle breeds than in humpless or European breeds. This result indicates that the missense mutation can be contributed the functional significance such as the signal transduction through the receptor binding also may be used as a marker for selection of the economic traits in Hanwoo.

사과의 감압저장법의 개발

손태화,윤형식,홍순영,서정훈,김광수,박용태,이갑랑,권태종 慶北大學校 生産技術硏究所 1970 生産技術 Vol.4 No.-

Rolls, Janathan, and American Summer Pearmain were used in this experiment. This experiment was conducted under 0˚ and 20℃ at subatmospheric pressure, respectively. we found to examine physiological changes of apple under storage and the rate of respiration of apple in order to decide the time of harvesting. The summary results were as follows. A. Physiological Changes of Apples Stored Under Reduced Atmospheric Pressure. 1. Rate of Respiration (1) Lower rates of respiration were observed under reduced atmospheric pressure than under normal atmospheric pressure other storage conditions being equal. (2) American Summer Pearmain apples showed the biggest rate of respiration, followed by Jonathan apples. Rolls apples showed the smallest rate of respiration among three varieties. (3) The rate of respiration of apples stored under reduced atmospheric pressure changed less remarkably than that of apples stored under normal atmospheric pressure when these apples were taken out of the storage rooms, and their surrounding temperature and atmospheric pressure were changed. (4) change of the rate of respiration was more marked in an O^2-chamber than in an air-chamber. A serious physiological breakdown was observed in a N_2-chamber and in a CO_2-chamber. 2. Production of Ethylene (1) The action of ethylene as a ripening hormone for apples was supressed by reducing the atmospheric pressure of the storage chamber. (2) The order of amount of ethylene production was American summer Pearmain, Jonathan, and Rolls. The ethylene production was larger under reduced atmospheric pressure than under normal atmospheric pressure without respect to the variety of apples. (3) Ethylene production of apples was reduced by lowering the temperature of the storage chamber (4) The rate of ethylene formation increased markedly when apples were stored in a cold chamber and than the temperature and atmospheric pressure were changed. This phenomenon, however, was less marked for the apples stored in the reduced atmospheric pressure chamber than for those in the normal atmospheric pressure chamber. (5) The ethylene production in the O_2-storage chamber was about 80 times as large as that in air-storage chamber at 20℃. The rate of ethylene production reached a maximum in 12 days in the O_2-chamber, but the ethylene production increased continuously in the air-chamber. In the N_2-chamber and the CO_2-chamber ethylene production was small. Generally the ethylene concentration in the atmosphere of the storage chamber was smaller under reduced atmospheric pressure than under normal atmospheric pressure. 3. Acidity Acidity was lowered from 0.68% to 0.36%, 0.50%, or 0.56% when Jonathans were stored in an uncontrolled atmospheric chamber, a cold and uncontrolled atmospheric pressure chamber, and a cold and reduced atmospheric pressure chamber respectively. 4. Change of Weight Decrease in weight under the same conditions as the above 3, was 6.2%, 3.6% and 2.8% when stored in an uncontrolled chamber, in a cold and uncontrolled atmospheric pressure, chamber, and in a cold and reduced atmospheric pressure chamber respectively. 5. Decaying Decaying ratio showed also the same order as 4, and was 18.5%, 7.4%, and 3.6% in an uncontrolled chamber, in a cold and uncontrolled atmospheric pressure chamber, and in a cold and reduced atmospheric pressure chamber respectively. 6. Enzyme Activity (1) Catecholase activity of apples decreased with storage duration. (2) The order of catecholase activity was Rolls, American summer Pearmain and Jonathan. (3) Catecholase activity was higher in apples stored under reduced atmospheric pressure than in those stored under normal atmospheric pressure regardless of the variety. B. Preliminary Investigations for Practical Uses 1. It is possible to calculate the concentration of carbon dioxide in a storage chamber atmosphers since the rate of carbon dioxide production was almost constant according to the storage conditions. Therefore the storage chamber was successfully maintained at 5% carbon dioxide by controlling ventilation. 2. With a simple automatic controller, the storage chamber atmosphere was maintained at an appropriate reduced pressure. 3. Poly ethylene film, which is most permeable for gases among several common plastic films, was found to be adoptable as an apple wrapper for reduced atmospheric pressure storage. C. Investigation of the Time of Harvest Observations of the rate of respiration of apple during growing made in possible to predict the optimal time of harvest. The optimal times of harvest are around September 23 and October 26 for Jonathan and Rolls apples respectively.

Magnetic resonance tracking of multifunctional nanoparticle-labeled mouse mesenchymal stem cells in a mouse model of myocardial infarction

Lee, K.,Park, C.,Moon, H.Y.,Ahn, E.,Park, H.E.,Ihm, S.H.,Seung, K.B.,Yoon, T.J.,Chang, K.,Lee, C.,Cheong, C.,Hong, K.S. Elsevier 2009 Current Applied Physics Vol.9 No.1

Magnetic ferrite nanoparticles (MNPs) are nano-sized magnets that distort the local magnetic field and alter the T<SUB>2</SUB> and T<SUB>2</SUB><SUP>*</SUP> of the water protons in the surrounding water molecules. This property renders MNPs a favorable negative MR contrast agent for biomedical applications. Silica coating and polyethylene glycolation confers additional stability and biocompatibility to the MNPs. Organic dyes incorporated into the silica shell enable the detection of MNP fluorescence by confocal laser scanning microscopy (CLSM). Mouse mesenchymal stem cells (mMSCs) in adult bone marrow can differentiate into cardiomyocytes. The mMSCs recruited in the region of myocardial infarction can express muscle-specific markers and increase the regional wall motion. Using MRI, we tracked the fate of MNP-labeled mMSCs injected into the infarcted myocardium. The mMSCs were effectively labeled with polyethylene-glycolated MNPs, as confirmed by CLSM. The MNP-labeled mMSCs were injected around and into the infarcted myocardium of C57/BL6 mice. Using MR, we successfully tracked the MNP-labeled mMSCs in the hearts of mice at 7d after inducing myocardial infarction. The localization of MNPs in the myocardium was validated by the in vivo and ex vivo MR images.

Steam reforming of n-dodecane over K₂Ti₂O₅-added Ni-alumina and Ni-zirconia (YSZ) catalysts

Kim, T.,Song, K.H.,Yoon, H.,Chung, J.S. Pergamon Press ; Elsevier Science Ltd 2016 International journal of hydrogen energy Vol.41 No.40

<P>The steam reforming of n-dodecane over 35 wt% Ni supported on alumina (Ni-Al2O3) and yttrium-stabilized zirconia (Ni-YSZ) at 800 degrees C for 10 h with a steam-to-carbon ratio of 3 was tested in the presence and absence of 10 wt% of K2Ti2O5 (KTO) particles. The suppression of coke formation by KTO was investigated using various characterizations, including BET, TPO, TGA, TEM, and EELS. After the addition of 10 wt% of KTO particles by ball milling, Ni-YSZ exhibited stable performance with negligible coke formation at space velocities of less than 20,000 h(-1). The addition of KTO to Ni-Al2O3 did not yield any improvements because the majority of Ni particles in alumina pores are not directly in contact with the KTO phase. The dispersion of finely divided KTO particles on the surfaces of both Ni and the support effectively suppressed coke formation by steam gasification of the deposited coke into CO and H-2. First, the gradual accumulation of coke during the course of the reaction deactivated the Ni sites, which presented activity for the steam reforming of methane. The Ni and acidic sites, which were effective for the hydrogenolysis of n-dodecane to methane and ethylene (or ethane in the presence of KTO), were eventually deactivated. An overall reaction pathway was then proposed based on the results. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.</P>