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HBx형질전환 생쥐에서 발생한 간세포암종에서 H-ras 및 c-myc의 발현에 관한 연구
문형배,소병준,김학철,윤기중,한원철,조향정,유대열,정영진 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2
<연구목적> HBx형질전환 생쥐에서 발생한 간세포암종의 발암과정에 종양유전자(H-rgs, c-myc)의 발현 정도를 조사하고자 하였다. <연구방법> 정상생쥐 12마리(4-18개월) 및 HBx 형질전환 생쥐 44마리(4-18개월)를 대상으로 포르말린에 고정하고 파라핀에 포매한 간 조직을 이용하여 면역조직화학적염색을 실시하였다. 실험군은 정상 부위, 이형성 부위 및 종양 부위로 구분하였으며, 종양 부위는 소결절성병변 부위와 간세 포암종 부위로 구분하였고, 이형성병변 부위는 이형성병변만 발견되는 부위, 소결절성병변과 동반된 이형성병변 부위 및 간세포암종과 동반된 이형성병변 부위로 구분하였다. <연구결과> H-rgs의 발현은 정상 간조직에 비하여 이형성병변 부위(P<0.05) 및 종양 부위(P<0.01)에서 증가하였으며, 소결절성병변 부위과 간세포암종 부위 사이에서는 간세포암종 부위에서 증가된 경향이었으나 통계학적으로 유의한 차이가 없었으며, 각 이형성병변 부위 사이에서도 유의한 차이는 없었다. c-myc의 발현은 정상 간조직 및 이형성병변 부위에 비해 종양 부위에서 증가하였으며(P<0.001), 소결절성병변 부위와 간세포암종 부위에서는 비슷하였고, 각 이형성병변부위 사이에서도 비슷하였다. <결론> HBx형질전환 생쥐에서 발생하는 간세포암종의 발생에 H-rgs는 이형성 변화를 일으키는 시기에 관여하며, c-myc은 이형성병변에서 암으로 이행하는 시기에 관여할 것으로 생각한다. Background: This experiment was designed for the expression of H-ras and c-myc in hepatocarcinogenesis of the HBx transgenic mice. Methods: Immunohistochemical stains in the paraffin embedded tissue of the liver were used for the detection of H-ras and c-myc in the 12 normal mice and 44 HBx transgenic mice of the 4-18 month old. Results: Expression of the H-ras was significantly increased in the dysplastic area (P<0.05) and tumor area (P<0.01) than in the normal liver. But there were no differences of H-ras expression between areas of microscopically identified hepatocellular carcinoma (MI-HCC) and grossly identified hepatocellular carcinoma (GI-HCC) and dysplastic areas among the only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Expression of the c-myc was significantly increased in the tumor area (P<0.001) than in the normal liver and dysplastic area. But there were no differences of c-myc expression between areas of MI-HCC and GI-HCC, and dysplastic areas among only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Conclusions: Our study suggests that H-ras is related to the dysplastic change and c-myc is related to the neoplastic change in the hepatocarcinogenesis of the HBx transgenic mice.
정소학,김성대,권영호,손정환,백선용 고신대학교 의학부 2000 高神大學校 醫學部 論文集 Vol.15 No.1
Background The morphological changes in the peripheral nerve and S-100 immunoreactivity were studied after axotomy. Methods Adult Sprague-Dawley rats received crush injury with aneurysmal clip in the sciatic nerve at mid-thigh level. The animals were cardiac perfused with 4% paraformaldehyde for cryostat section and half Karnovsky fixative for semithin section from 6 hours to 4 weeks after crush injury. The immunohistochemistry of Schwan cells was performed. Results Degeneration of myelin sheath and endoneurial edema were observed at 6 hours. Macrophages were invaded into the basal lamina tube and phagocytosed the destructed axons and myelin sheath at 3 days. Proliferation of Schwann cells in the basal lamina tube was observed at 5 days group. Thin myelinated nerve fiber and degeneration of myelin sheath were observed at 2 weeks. Conclusion These results indicate that after crush injury of the peripheral nerve regeneration of myelin sheath and Schwan cells is partially completed in 1 week.
정소학,김재도,권영호,손정환,장재호,정구희,차상원 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.1
Purpose : To analyze the prognosis and clinical results through retrospective survey with classification of benign tumors in the hand. Materials and methods : Patients undergoing surgical treatment with diagnosis of hand tumors from March 1990 to February 2006 were reviewed. The incidences of occurrence, the location of tumors, treatment methods, clinical results, and prognosis were compared and analyzed. Results : The subjects consisted of 131 cases of benign tumors. Soft tissue tumors were 103 cases consisted of 38 cases of ganglion that took the largest share and 15 cases of hemangioma that took the second. Bone tumors were 28 cases and 15 cases of enchondroma took the largest share. The mean period until manifestation of symptoms was 20.5 months and 43 cases had not longer than 6 months of period. In terms of main symptoms at first the examinations, manifestation of palpable mass were most frequent with 76 cases and 27 cases showed painful mass as the major symptoms. In all cases, surgical treatment and histological examination; marginal excision were the most frequent with 116 cases and curettage and bone graft were 16 cases, respectively. Conclusion : Of the 131 tumor patients treated for 17 years, the authors diagnosed 131 cases as benign; bone tumors took 21.3% and enchondroma were the most frequently occurred. Soft tissue tumors took 78.8%; of them, ganglion were the most frequent and hemangioma and glomus tumor showed high incidences. Benign tumors occurred in the hands may be treated with intra-lesion excision or marginal excision.
So-Young Huh,Su-Hyun Kim,Ki Hoon Kim,Young Nam Kwon,Sung-Min Kim,Seung Woo Kim,Ha Young Shin,Yeon Hak Chung,Ju-Hong Min,Jungmin So,Young-Min Lim,Kwang-Kuk Kim,Nam-Hee Kim,Tai-Seung Nam,Sa-Yoon Kang,Je 대한신경과학회 2022 Journal of Clinical Neurology Vol.18 No.6
Background and Purpose Fingolimod (FTY) inhibits lymphocyte egress from lymphoid organs to cause lymphopenia, but the clinical implications of FTY-induced lymphopenia are not fully understood. We aimed to determine the frequency and severity of lymphopenia during FTY treatment among Korean patients with multiple sclerosis (MS), and its association with infections. Methods We retrospectively reviewed the medical records of patients with MS treated using FTY from 12 referral centers in South Korea between March 2013 and June 2021. Patients were classified according to their nadir absolute lymphocyte count (ALC) during treatment: grade 1, 800–999/μL; grade 2, 500–799/μL; grade 3, 200–499/μL; and grade 4, <200/μL. Results FTY treatment was administered to 69 patients with a median duration of 18 months (range=1–169 months), with 11 patients being treated for ≥7 years. During FTY treatment, mean ALCs were reduced after the first month (653.0±268.9/μL, mean±standard deviation) (p<0.0001) and remained low during treatment lasting up to 84 months. During follow-up, 41 (59.4%) and 7 (10.1%) patients developed grade-3 and grade-4 lymphopenia, respectively. No significant difference was found in age at FTY initiation, sex, baseline ALC, body mass index, or prior disease-modifying treatment between patients with and without grade-4 lymphopenia. Infections were observed in 11 (15.9%) patients, and the frequencies of patients with and without grade-4 lymphopenia were similar. Conclusions FTY treatment induced grade-4 lymphopenia in 10% of South Korean patients with MS, but did not appear to be associated with an increased infection risk.
Kim, So Yeong,Joo, Young Min,Lee, Hak Sung,Chung, In-Sik,Yoo, Yun-Jung,Merrell, D Scott,Cha, Jeong-Heon Springer Science and Business Media LLC 2009 The Journal of Antibiotics Vol.62 No.1
<P>Resistance to metronidazole (MTZ) in Helicobacter pylori is associated with mutations in rdxA, encoding an oxygen-insensitive NADPH nitroreductase, and mutations in frxA, encoding a NAD(P)H-flavin oxidoreductase. Despite this association, the strict correlation of MTZ resistance with mutations in rdxA or frxA is still controversial. In this study, rdxA allelic replacement was used to distinguish resistance-associated nucleotide mutations from the natural genetic diversity of H. pylori. Replacement with truncated rdxA resulted in MTZ resistance, whereas replacement with missense-mutated rdxA from resistant clinical isolates failed to yield MTZ resistance. Thus, although truncation of rdxA confers MTZ resistance in G27 H. pylori, MTZ resistance found in other clinical isolates is not due to the identified amino-acid substitutions. Three of our MTZ-resistant clinical isolates expressed functional rdxA and two of these also encoded full-length frxA. Therefore, MTZ resistance can arise in H. pylori possessing functional rdxA, suggesting that other factors are involved in MTZ resistance.</P>
폐경후 여성에서 에스트로겐 치료에 대한 혈중 지질의 반응과 에스트로겐 수용체 다형성과의 관계
황정규,한인권,장학철,임창훈,정호연,한기옥,윤현구,박재은,박소라,정필호 대한내분비학회 1999 Endocrinology and metabolism Vol.14 No.3
Backgound: Several biologically plausible mechanisms have been proposed for estrogen-mediated caridoprotection, including estrogen-assocaited changes in lipid metabolism and endothelial function of vessel walls. These effects are thought to be mediated via estrogen receptor (ER). Relationships between ER polymorphisms and serum lipid levels were not investigated enoughly. Methods: Three restriction fragment length polymorphisms (RFLPs) at the ER gene locus, represented as B-variant, PvuII and XbaI, and their relationship to serum lipid levels were examined in 318 postmenopausal women. Their mean age was 54.5±6.5 years (mean+SD). An association between ER genotypes and changes in lipid levels after 1 year of estrogen replacement therapy was also investigated in follow-up 251 women. Results: The B-variant was not found in Korean women. The distribution of the PvuII and XbaI polymorphisms was as follows: PP 109 (34%), Pp 166 (52%), pp 43 (14%), and XX 204 (64%), Xx 95 (30%), xx 19 (6%). Significant relationshipwas found between genotypes and changes in serum total cholesterol levels after lyr estrogen replacement therapy. There was no significant relationship between ER genotypes and changes in HDL cholesterol, LDL cholesterol and triglyceride levels after estrogen therapy. Conclusion: These data indicate that these polymorphisms are possible predictor on lipid response to estrogen replacement therapy (J Kor Soc Endocrinol 14:553-561, 1999).