과열증기처리 반탄화 추출물의 항산화 효과 검증에 관한 연구

오근혜(Geun Hye Oh),남정빈(Jeong Bin Nam),양승민(Seung Min Yang),정원희(Won Hee Joung),정진산(Jin San Jeong),신종민(Jong Min Shin),강석구(Seog Goo Kang) 한국펄프·종이공학회 2018 펄프.종이기술 Vol.50 No.2

The antioxidant properties of the extracts from torrefied wood subjected to superheated steam treatment were investigated. Total polyphenolics, total flavonoids, DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity, reducing power, and FRAP (Ferric reducing antioxidant power) were determined to evaluate antioxidant activity. The total polyphenol content was 879.67±40.41 ㎎/mL at a concentration of 25 g/mL when the extract was subjected to a temperature of 300℃ for 10 min, and 759.67±25.17 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value of at 300℃ was 15.79% higher than that obtained at 350℃. Total flavonoid content was 111.18±3.55 ㎎/mL at a concentration of 25 g/mL at 300℃ for 10 min, and 80.58±2.58 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 37.97% higher than that obtained at 350℃. For the DPPH free radical scavenging activity, the highest scavenging activity was observed at a concentration of 6 g/mL. DPPH free radical scavenging activity at a concentration of 6 g/mL at 300℃ for 10 min was 89.83±0.03%, whereas at 350℃ for 10 min at the same concentration, it was found to be 87.99±0.1%. The value at 300℃ was 2.09% higher than that obtained at 350℃. Reducing power was determined to be 3.59±0.04 at a concentration of 25 g/mL at 300℃ for 10 min, and 2.92±0.1 at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 22.94% higher than that obtained at 350℃. FRAP was 1742±37 μM at a concentration of 25 g/mL at 300℃ for 10 min, and 1106±8 μM at a at 350℃ for 10 min. At 300℃, the FRAP value was 57.50% higher than that obtained at 350℃. Based on these results, we suggest that torrefied wood treated with superheated steam can be used in various applications because of its effective antioxidant properties.

대장내시경검사의 전처치로서 항콜린제 사용에 관한 연구 : 전향적, 이중맹검법으로

박경남,한동수,이민호,최호순,박준용,손주현,이오영,함준수,전용철,송승찬,기춘석,윤병철,이종희 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.3

Background: Use of antispasmodic medication prior to colonoscopy is controversial but someone believes antispasmodic may improve visualization of colonic mucosa and ease colonoscope insertion. So, we designed a study to assess the effect of premedication with the antispasmodic, hyoscine-N-butyl bromide(Buscopan') on the performance of colonoscopy. Methods: This study was prospective, double blinded, randomized, controlled study, One hundred three consecutive patients were randomized to receive intravenous buscopan lml(n=52) or placebo(n=51) combined with our standard initial medication(me- peridine 50 mg and midazolam 2 mg). Insertion of colonoscopy was timed, and 100 mm visual analogue scales (VAS) were used for asscssing difficulty of procedure, colonic motility, frequency of positional change, frequency of external compression, difficulty of assistance and degree of discomfort experienced by the patients. Results: There were no significant differences of intubation time between buscopan group(mean time, 7.23 min., range 2~15) and placebo group(7.07 min., range 3-25), (p=0.83) and withdrawal time between buscopan group (6.46 min., range 2-22) and placebo group(6.76 min., range 2 25), (p=0.69). Also, there was no significant differences in intubation time between males and females(buscopan; males 7.00 min., females 7.60 min., p=0.34, placebo; males 7.0~5 min., females 7.08 min., p 0.44). The VAS scores checked by endoscopist(p=0.29), assistant(p=0.32) and patient (p=0.15) were not significantly different in both groups. There were no significant differences in intubation time, VAS scores nf endoscopist, assistant, and patients. Conclusion: Premedication with intravenous bu.opan has no advantage on colonoscopy procedure. Use of antispasmodic medication prior to colonoscopy was not considered as recommendable agent.

실내 스모그 챔버의 설계 및 성능평가

배귀남,김민철,이승복,송기범,진현철,문길주 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.4

A multi-functional indoor smog chamber was designed and evaluated lo investigate photochenical or water vapor reaction mechanisms of air pollutants. Various smog chamber experiments could be conducted using ambient air or purified air in this smog chamber. The smog chamber consisted of a housing, a Teflon bag, blacklights, injection ports, sampling ports, and utility facilities. The characteristics of light source, the wall losses of air pollutants, and the quality of purified air were experimentally investigated. The maximum NO₂ photolysis rate was 1.10min^(-1). In a 2.5-m³ Teflon bag, the wall losses of ambient O₃, NO, and NO₂were 1.2~2.4×10^(-3)min^(-1), 0.7~2.0×10^(-3)min^(-1), and 0.4-2.0×10^(-3)min^(-1), respectively. The wall loss of ambient particles ranging 0.05 to 0.2㎛ was 1.8~5.4×10^(-3)min^(-1), which was slightly higher than those of ambient gaseous species. The purified air supply system provided high quality of air with NO₂, < 1 ppb, and total hydrocarbons <5 ppb.

남자 중량급 유도선수들의 체지방량 추정에 관한 연구

남덕현,민성호 龍仁大學校 武道硏究所 2004 武道硏究所誌 Vol.15 No.1

The purpose of This study aims to examine prediction of the body fat weight in judo middle weight players. The experiment was conducted on the 30 male judo middle weight players in yong in university. The 3 types of calipers of Harpenden, Skyndex and Lang are used to measure the 4parts, and then I calculated the reliability, the validity and compared the difference in the measurements between the types with the result. The average and the standard deviation of all the result of the experiment were calculated by SPSS v.11.0 for Windows. With the data, I examined the difference of the reliability (represented by Pearson 's r) between the types, and that of validity with the correlation coeffients by a part. As well, I compared the difference of the measurements by a part between the types using the One-way ANOVA. I found out the following through the analysis. First, There is a significant difference in the averge of the repetea measurements of body 4 parts in Harpenden and Lange, Skyndexand Lange. second, The harpenden prediction of the body fat weight calculated from the measurements were as follows: Y=15.982+(Triceps×0.321)+(Front thigh×0.229)+(Subscapular×0.936)-(Abdominal x1.092) Third, The skyndex prediction of the body fat weight calculated from the measurements were as follows: Y=15.610+(Triceps×0.349)+(Front thigh×0.221)+(Subscapular×0.939)-(Abdominal ×5.658) Forth. The Lange prediction of the body fat weight calculated from the measurements were as follows: Y=15.417+(Triceps×0.327)+(Front thigh ×0.196)+(Subscapular×0.851)-(Abdominal×0.950)

냉동 건조 탈회 동종골 이식시 조직 접착제가 골 치유 과정에 미치는 영향에 관한 실험적 연구

민승기,김수남,김치경 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.4

Allogeneic bone grafting has recently been used in oral and maxillofacial regions to restore the cosmetic and functional problem. There are several types of allogeneic bone grafts ; bone powder, bone chips, bone blocks. Empirically, it is thought to be better to combine the allogeneic bone chips to any type of tissue adhesive not to displace during packing and condensing. But, there are no reports about using tissue adhesive in allogeneic bone grafting. This experimental study is designed to investigate the effect of the fibrin adhesive on bone healing process after demineralized allogeneic bone grafting in 60 rats. In control groups (30 rats), routine demineralized allogeneic bone grafting were done in 7×7mm calvarial bone defects which were drilled intentioally. And we used the fibrin adhesive for holding the bone particle in experimental groups 930 rats). Each experimental specimen was sacrified at 1, 2, 4, 6, 8 weeks postoperatively The results were as follows : 1. The degree of inflammatory cell infiltrations were more prominent in experimental than in control groups till 2 weeks. 2. Early fibroblast proliferation and new capillary proliferation were uncorporated around graft sites in the experimental groups later than in control groups at early stages. 3. Osteoblastic activity in control group was more prominent at 2 weeks. 4. Osteoblastic activity in experimental groups was more prominent than in control group till 4 weeks. 5. New bone formation was more in control group than experimental group till 3 weeks, but similar appearance after that time. As above results, initial bone healing within 2 weeks were more processed in without adhesive group than with adhesive group. But above 4 weeks; similar bone healing were observed.

한국인 전상환자의 악안면결손에 대한 임상적연구

민승기,김수남,최규환,엄인용 大韓顎顔面成形再建外科學會 1992 Maxillofacial Plastic Reconstructive Surgery Vol.14 No.4

Maxillofacial war injures is specific representative of severe hard and soft tissue defect. This type of injuries were different from the trauma because it may be fatal. The purpose of this study was to evaluate the injury type base on the retrograde medical record in the 104 patients from Feb. 1991 to Aug. 1992 in Korea Veterans Hospital. The obtained results were as follows. 1. Among 104 cases, 51 cases(49.0%) were classified as mandibular defects only, and 53 cases (51.0%) were classified as maxillary with mandibular defect (combined). 2. The etiologic factors of injury were gunshot, artillery and grenade or shell: 33 cases, 14cases, and 10 cases respectively in Korea War, 19 cases, 5 cases, and 8 cases respectively in Vietnam War. 3. In 57 cases of mandibular partial defect, the angle area showed the highest frequencies, 18 cases(31.6%). 4. In 42 cases of mandibular segmental defect, the area between the 1st molar and the ascending ramus showed the highest frequencies. 17 cases(40.4%), and almost all cases were unilateral defect (40 cases, 95.0%) 5. Reconstruction method performed for segmental mandibular defect were wire or plate(15 cases, 35.7%) and soft tissue closure only(12 cases, 28.7%), respectively.

다양한 적송잎 추출물의 항생제 내성 황색포도상구균에 대한 항균활성 검증

Nam-Young Kim(김남영),Min-Kyung Jang(장민경),Myung Je Jeon(전명제),Dong-Geun Lee(이동근),HyeJi Jang(장혜지),Seung Woo Lee(이승우),Mihyang Kim(김미향),Sung Gu Kim(김성구),Sang-Hyeon Lee(이상현) 한국생명과학회 2010 생명과학회지 Vol.20 No.4

병원 내 감염의 주된 원인균으로 알려진 황색포도상구균(5. aureus)과 항생제 내성 황색포도상구균(PRSA, MRSA)에 대해 항균활성이 우수한 천연 항균성 물질을 검색하기 위해 적송잎을 대상으로 열수-헥산(HWH), 열수-에탄올(HWE), 헥산, 에탄올 및 열수 등으로 획득된 추출물들로 항균활성을 조사하였다. 헥산, 에탄올, 열수-헥산, 열수-에탄올 추출물들은 그람양성 대표세균인 고초균(B. subfilis), 황색포도상구균 표준균주, 항생제 내성균주인 PRSA 및 MRSA에 대해서 항균활성을 나타내었으며, 특히 MRSA (ATCC 33591) 균주에 대해 다른 균주들 보다 높은 항균활성을 나타냈다. 열수 추출물은 황색포도상구균 표준균주 및 MRSA에서만 항균활성을 나타냈지만, 항균활성은 가장 높아 50 ㎎/㎖의 농도에서 33 ㎜의 생육저해환을 나타냈다. 각 균주들에 대한 생장의 최소저해 농도(MIC)는 HWH 및 HWE 추출물이 모두 0.05 ㎎/㎖의 농도로 가장 낮았고, 열수추출물이 0.5 ㎎/㎖의 농도였으며, 에탄올 추출물은 5 ㎎/㎖의 가장 높은 농도를 나타냈다. 모든 추출물들은 121℃에서 20분간의 열처리 후에도 항균활성이 유지되었으므로 열에 대해 안정한 물질임을 알 수 있었다. 본 연구결과로 식품산업에서 솔잎 추출물을 천연 항균제로 사용할 수 있을 것으로 기대할 수 있다. investigated antimicrobial activities of various pine (Pinus densiflora) needle extracts against antibiotic resistant strains of Staphylococcus aureus. Hot water extract showed the highest antimicrobial activity against normal and methicillin resistant Staphylococcus aureus (MRSA), however, it exhibited no antimicrobial activity against penicillin resistant S. aureus (PRSA). Hot water-hexane (HWH), hot water-ethanol (HWE), hexane, and ethanol extracts showed antimicrobial activity against S. aureus, PRSA and MRSA. Minimum inhibitory concentrations (MIC) of HWH, HWE, hexane, and ethanol extracts were 0.05, 0.05, 0.5 and 5 ㎎/㎖ respectively, and HWH and HWE extracts showed the strongest antimicrobial activity among these extracts. Antimicrobial activities of pine needle extracts were stable after heating at 121℃ for 20 min. These results suggested that pine needle extracts can be used as an effective natural antimicrobial agent for food and medical industries.

수술실 손 소독제의 종류에 따른 균주 수의 변화

홍성윤,김정민,김소영,이수정,오은실,양서인,김화실,김남초 병원간호사회 2006 임상간호연구 Vol.11 No.2

Purpose: The present study purposed to compare the hand washing effect of 7.5% powidone-iodine, which is used in the operation room of C university hospital in Seoul, with that of Ethyl alcohol-CHG mixture recommended by the US Association of Operating Room Nurses (2002) and to adopt a more effective hand disinfectant. Method: In a quasi-experimental design, 48 medical staff who participated in operations during the period from November 2004 to February 2005 had hand washing using the two kinds of hand disinfectants: 7.5% povidone-iodine and the ethyl alcohol-CHG mixture. Their palms were swabbed and cultured just after hand washing and again after taking dff sterile gloves after the operation. The number of colonies from the two occasions were counted and compared. Result: The number of general bacillus increased significantly in the group using 7.5% povidone-iodine compared to that in the group using the ethyl alcohol-CHG mixture. The number of general bacillus increased signficantly in doctors compared to that in nurses. The factors affecting the increase of the number of general bacillus were disinfectants and medical personnel. The number of general bacillus was expected to increase 9.41 times with 7.5% povidone-iodine than with the ethyl alcohol-CHG mixture and 14.87 times in doctors than in nurses. Conclusion: This study shows that the ethyl alcogol-CHG mixture has a stronger hand disinfection effect than 7.5% povidone iodine. Thus we need to change the hand disinfectant used in operating rooms as soon as possible in order to minimize the infection of wounds resulting from operations.

랫드에서 l-muscone의 급성독성 및 아급성독성시험 연구

오승민,연제덕,남혜운,박대규,조명행,정규혁 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

l-Muscone is synthesized for use as substitutive material of musk which is the active ingredient of woohwangchungsimwon. The objective of this investigation was to evaluate the acute and subacute toxicity of l-muscone in rats. In oral acute toxicity test, SPF Sprague-Dawley male and female rats were gavaged with l-muscone of two doses(0, 5.0g/㎏). No dead animal and abnormal autopsy findings were found in control and treated group. Body weights were slightly decreased in both sexes of rats treated with 5.0g/㎏. Therefore, oral LD_50 of l-muscone was consider to be higher than 5.0g/㎏ in male and female rats. In intraperitoneal acute toxicity test, rats were injected intraperitoneally with dosages of 0, 1,000, 1,316, 1,732, 2,279 and 3,000 ㎎/㎏. Decreased body weights and motor activities were observed at high dose group. Intraperitoneal LD_50 of l-muscone were 1,920 ㎎/㎏ in male and female rats. In the subacute study. l-muscone was administrated orally to both sexes of rats for 4 weeks as several doses(0, 10, 100 and 1,000 ㎎/㎏). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data suggest that no observed adverse effect level of l-muscone in rats might be over 1,000 ㎎/㎏/day in this study.

응급의료센터의 단기입원치료실 활용경험

최승필,박승현,정시경,박규남,김영민,김세경,이운정,이환 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Overcrowding of emergency department is a serious and growing problem at St. Mary's Hospital. This has motivated the development of short-stay unit(SSU) as an alternative ward to routine hospital admission and ED discharge. In our hospital a SSU begun to admit patients in January 6, 1997. The SSU received 247 patients from the ED during the eight month interval(January 6, 1997 to August 31, 1997). To examine the utilization of the SSU in ED, we retrospectively analyzed 247 patients admitted in SSU from the ED, and compared the average hours per patient with acute gastroenteritis spent in the ED during the 2-month intervals before(July-August 1996) and after(July-August 1997) the establishment of the SSU. The following results were obtained; 1. The total patients consist of EM 92(37.2%), GS 48(19.4%), IM 24(9.7%), PS 21(8.5%), OS 20(8.1%), OBGY 14(5.7%) and others 28(11.3%) 2. The results of EM patients admitted in SSU from ED 1) Sex ratio of male to female was 1:1.1 and the mean age was 37.3±16years 2) In diagnosis, acute gastroenteritis was 29 cases(31.5%), multiple contusion 14 cases(15.2%), drug intoxication 12 cases(13%), limb laceration 6 cases(6.5%), tendon rupture of hand 6 cases(6.5%), and others 19 cases(20.6). 3) Mean length of stay in ED was 9.18 hours. 4) Among 92 patients to the SSU, 79 patients(85.9%) were discharged, 11 patients(11.9%) formally admitted to hospital and 2 patients(2.2%) transferred to otherhospital. The mean hospital stay time of the patients admitted to SSU was 2.6 days. 3. There was a significant reduction in the average stay time spent in the ED by treat-and-releasing patients with acute gastroenteritis after the establishment of the SSU(from 14.65±9.6 to 7.52±5.4 hr/patient, p<0.001). Conclusively, the establishment of the SSU can shorten the average stay time that treat-and-releasing patients spend in the ED, and reduce the number of admitted patients waiting in the ED.