카드뮴투여가 흰쥐 고환의 조직변화와 단백질변화에 미치는 영향

최송표,김수일,조근자,양은진,김선,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

This study was done to investigate histochemical and protein changes in the rat testis I WAT and 3 WAT with 4 mg/kg of cadmium, IP, single injection. A control group and 2 experimental groups were studied. Testes were processed routinely for H&E stain and agarose gel electrophoresis. The results were as follows ; 1. In the histochemical studies, death of all the spermatogenic cells and Sertoli cells in the seminiferous tubules, and decreased ground substance, decreased fibroblasts and interstitial cells and increased inflammatory cells in the interstitial tissues were found in the cadmium treated groups, and the degree of necrotic changes was more severe in the 3 WAT group. 2. In the biochemical studies, two kinds of proteins, 25 kDa and 45 kDa, were disappeared and one kind of protein, 100 kDa, was decreased after Cd treatment. 25 kDa and 45 kDa proteins were suggested as actin which consist the spermatogenic cells, but what's the protein 100 kDa is unclear. According to the above results, it is considered that there would be irreversible changes in seminiferous convoluted tubules and also in the interstitial tissues after cadmium treatment, and detecting amino acid sequences of these proteins would help understanding the mechanism of necrosis in the testis tissue after cadmium administration.

컴포머에 대한 복합레진의 전단결합강도에 관한 연구

정송란,최남기,양규호,김선미,송호준 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.3

컴포머(compomer) 충전물이 파절될 경우 즉, 컴포머 수복이 실패했을 때 기존 컴포머의 파절된 부분(aged compomer)을 복합레진으로 수복해야 하는 경우를 고려해야 한다. 본 연구에서는 컴포머의 표면처리 방법에 따른 수리(repair)를 위한 복합레진과의 전단결합강도 변화를 알아보기 위해 두 개의 mold를 만들어서 Dyract AP, F2000를 컴포머 수복재료로, Z250을 수리용 복합레진 재료로 사용하여 컴포머와 레진을 부착시킨 시편을 제작하였다. 컴포머와 복합레진의 종류 조합, 본딩레진 사용유무, 열순환 처리 유무, 표면거칠기 부여 유무, 기존 수복물의 시효(aging)에 따라 10개의 군으로 나누어, 컴포머와 복합레진간의 전단결합강도를 측정하고 그 파절양상을 관찰하여 다음과 같은 결과를 얻었다. 1. 컴포머의 복합레진에 대한 전단결합강도를 측정한 결과, Dyract AP가 F2000 보다는 높은 전단강도 값을 보였으나 통계적으로 유의한 차이는 없었으며 (p>0.05), fresh 컴포머군이 aged 컴포머군보다 결합강도가 높았다(p<0.05). 2. 각 군간의 전단결합강도를 측정한 결과 Dyract AP에 접착제를 도포하고 Z250을 접착시킨 제 5군이 가장 높았으며, Dyract AP를 충전하고 1주일간 물에 보관한 후 건조시켜 표면을 roughening 한 후 Z250을 충전시킨 제 9군에서 가장 낮았다(p<0.05). 3. 열순환을 시행한 군이 시행하지 않은 군보다 전단결합강도가 낮게 나타났으나 통계적으로 유의한 차이는 없었다. 4. 각 재료들의 결합실패가 일어난 파절단면을 조직학적으로 관찰한 결과, 전반적으로 접착파괴를 보였으나, 접착제를 도포한 군에서는 응집파괴가 우세하게 나타났다. 이상의 결과 fresh 컴포머 위에 접착제를 도포한 다음 수리용 복합레진 재료를 사용함으로써 컴포머와 복합레진간의 결합력을 증가시킬 수 있음을 알 수 있었다. 그리고 fresh 컴포머 위에 복합레진을 즉시 충전한 경우가 높은 결합력을 얻을 수 있었다. Aged 컴포머와 복합레진간의 성공적인 결합력을 얻으려면 aged 컴포머의 표면을 mechanically roughening하고 접착제를 도포한후 복합레진을 충전해야 할 것으로 사료된다. For the purpose of comparing the bond strengths of compomers to composite resin, composite 2250, and two polyacid modified composite resin, Dyract AP and F2000, were selected and investigated using universal testing machine for measuring the shear bond strengths. Additionally, the failure modes were examined by observing the fractured surfaces of each specimen. The following results were obtained. 1. The shear bond strength of Dyract AP to Z250 were higher than those of F2000, but there was no statistically significant difference between group 1 and group 3(p>0.05), and groups using fresh compomers showed higher bond strength than those using aged compomers(p<0.05). 2. After measuring the shear bond strength of each group, it was highest in group 5 and was lowest in group 9(p<0.05). 3. Although there was no statistically significant difference, groups treated with thermocycling showed lower bond strengths than those of non-thermocycling groups. 4. Overall compomer/composite resin failures were adhesive. Cohesive failures occurred mainly in groups using bonding agent. Based on these results, the application of a bonding agent on fresh polyacid-modified resin composite increases the bond strength between polyacid-modified resin composite and composite resin. Additionally, the surface of aged polyacid-modified resin composite has to be roughened mechanically and a bonding agent has to be used in combination with composite resin.

皇南大塚 新羅冠의 技術的 系譜

李松蘭 한국고대사학회 2003 韓國古代史硏究 Vol.31 No.-

The Silla Crown was made of thin sheets of gilded metal, cut and smoothed using a technique inherited from traditions deriving from the West. This study explores the technical lineage of the Silla Crown, by considering two elements: the gold spangles and many-sided gold beads. I would suggest two propositions about technical elements. First, the crown decorated with spangles, which were made of thin sheets of gold, was accepted from Central Asia through Silkroad. It originated in Bactriau culture. Through the commercial contacts between the Bactrian people and Xianbei(鮮卑) in the Northern China, the crown decorated with spangles was introduced into North China. Silla accepted this technique via Koguryo. Besides the gold spangles, the Crowns of Bactria, Xianbei and Silla have a common element, that is, tree-shaped uprights of which all segments are hand-cut from thin sheet gold. The Bactrian Crown is designed in the form of stylized tree. The uprights of the Xianbei Crown consist of two elements: a pentagon and a tree. Its shape is related to their legendary mountain. The Silla Crown has tree-shaped uprights with 7 or 9 boughs that are highly stylized on the front and sides and two antler-shaped uprights in the rear. Though they have same tree motifs, there are differences according to their inherent cultures. Related to the routes through which the crown decorated with the gold spangles are transmitted, some significant issues are raised. The route for gold spangle coincides with the gold trade route of Silla. Silla grew into a full-fledged kingdom with hereditary kings from the Kim family in the fourth century. Under the rule of Malipkan(麻立干), the gold ornaments began to be worn as the sign of their prestige and was introduced to international society through Koguryo. At that time, golds of Silla wer important items of international trade. Last, we would consider the many-sided gold beads. This technique was applied to two small golden chains, imitations of braids, hanging from either side of the headpiece at the level of the ear. These beads are made with filigree works. Filigree works developed differently according to regional cultures. Through the prosperous sea route from India to southern area of China near to coast, these beads, which were made with small gold rings, were popular for necklaces. From Han(漢) to East Jin(東晋), many-sided gold beads were produced in southern part of China. However, this type of beads was never excavated in the northern part of China. So, we could conclude that many-sided heads were characteristic of filligree works which were transmitted through the East Jin to Three Kingdom. In Korea, many-sided beads appeared as the daggling of earings at Koguryo. And then, in Silla, these beads were used as pendents and earings of the gold crown. In examining the origins of technical lineage of the gold crown, some considerations must be kept in mind: Although Silla actively accepted various international techniques. she took them in her own manner reflecting her cultural surroundings.

古新羅 古墳出土 土偶裝飾土器에 보이는 南方的 要素

李松蘭 동아대학교 박물관 2000 考古歷史學志 Vol.16 No.-

지문과 정신분열증

우숙희,정국동,최송표,조근자,양은진,김수일,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Fingerprint patterns of 333 schizophreniacs who met with the diagnostic criterias of International Pilot Study of Schizophrenia except items associated with chronicity in exclusion criteria were studied, and the following results were obtained. 1. The frequency distribution of the fingerprint patterns in the 333 schizophreniacs was ulnar loop, whorl, twinned loop, arch, radial loop in order in both control and schizophrenia groups. 2. Finger ridge counts of both sexes were decreased significantly in schizophrenia group. 3. Dissociation of fingerprint pattern in the schizophrenia group was 51.1%, which was far greater than 6.25% of control group. Number of dissociation was greatest in the thumb, followed by index, middle, ring, and little finger in order. According to the above results, it is considered that both the number of finger ridges and degree of dissociation of fingerprint patterns were closely related with schizophrenia. Analysis of fingerprints and palmprints with the aid of chromosomal analysis would contribute the early diagnosis and prevention of schizophrenia.

타오란[陶然] 작품에 나타난 홍콩의 장소성

송주란(Song, Ju-Ran) 부산대학교 인문학연구소 2015 코기토 Vol.- No.77

홍콩은 영국의 식민화 과정을 거쳐 기존에 공동체가 형성되지 못했던 무의미한 공간에서 유의미한 장소로 바뀐 대표적인 도시 중 하나이다. 식민지 이전까지 하나의 공동체로 형성되지 못했던 공간으로서의 홍콩은 영국인을 비롯한 서양인 이주민과 중국인 이주민의 유입으로 점차 하나의 공동체를 형성하게 되고, 중국과는 상대적으로 이질적인 성격의 정치?경제?문화?사회를 특징으로 하는 홍콩만의 독자성을 보유한 장소로 변모하게 된다. 이러한 홍콩의 가장 큰 특징은 혼종과 다중 정체성을 들 수 있고, 홍콩 문학에서도 이러한 현상은 쉽게 찾아볼 수 있다. 특히 홍콩 작가 타오란은 그의 작품에서 자신이 경험하고 체득한 홍콩의 특정 장소들에 대한 의미를 과거를 통한 회상과 기억의 방식으로 풀어내고 있는 것이 특징이다. 타오란은 홍콩 문학계에서 남래(南來)작가, 즉 이주 작가로 분류된다. 이는 타오란의 다중적인 신분?문화적 정체성을 보여줌과 동시에 경계인으로서의 특징들을 보여주는 이정표가 될 수 있다. 인도네시아 화교 출신인 타오란은 인도네시아에서 어린 시절을 보낸 후 조국인 중국에서 중?고등학교와 대학을 졸업하게 되고 그 후 피치 못할 이유로 홍콩에 거주하게 된다. 그 당시 그는 물리적 외압에 의해 홍콩으로 쫓겨 온 신세였고, 홍콩은 그에게 있어 단지 잠시 머물다 가야 할 의미 없는 공간에 불과했다. 하지만 그는 점차 의미 없는 공간으로서의 홍콩의 장소들에 대해 삶과 연결된 의미를 부여하게 되고, 그런 과정을 통해 공간 홍콩은 그에게 의미 있는 ‘장소’의 개념으로 인식하게 된다. 그의 작품에는 이런 장소들이 어떻게 자신에게 기억되고 있는 지를 보여주고 있다. 결국 타오란에게 홍콩은 자신의 삶이 영위되는 장소이자 언젠가는 돌아가야 할 곳으로 인식된다. 그리고 이미 그에게 안정된 장소로서의 홍콩 너머에 있는 광대한 공간 즉, 인도네시아와 베이징은 동경의 대상으로 자리 잡고 있다. 이러한 현상을 타오란의 작품을 통해 구체적으로 분석해 보고자 하였고, 타오란이 체험한 홍콩의 구체적 장소들에 대한 의미를 고찰하고자 하였다. Hong Kong is one of the representative cities that changed from a meaningless place to a meaningful one through citizenization policy implemented by Britain. While it could not form a single community before colonization, Hong Kong gradually developed into a single, distinct community as immigrants from China as well as from the West, including Britain, came into the city. Through this process, Hong Kong transformed into a unique place with local impacts, characterized by politics, economy, culture, and the society that are relatively heterogeneous compared to China. Such characteristics of Hong Kong can be found in Hong Kong literature as well. Hong Kong writer, Tao-Ran, is especially characteristic of writing his prose and novels about the local significance of Hong Kong through the reminiscence and recollection of his past. Tao-Ran came from China, who is classified as a migrant writer in the Hong Kong literature. This reveals Tao-Ran’s multiple positional identity and physical marginality. He was forced to live in Hong Kong by external pressure. Thus, for Tao-Ran, Hong Kong was merely a place for his temporary stay. However, he gradually imparted meaning to Hong Kong as a distinct place, and he shows this development of sense of space through his writings. Now, for Tao-Ran, Hong Kong is perceived as a space in which he goes about his daily life and to which he must go back some day. Furthermore, Tao-Ran starts to have a yearning for Indonesia and Beijing that are located in the vast space beyond the stable city, Hong Kong. This study attempted to specifically shed light on this phenomenon through Tao-Ran’s prose and novels and to consider the local meaning of Hong Kong that Tao-Ran experienced.

Enzyme-assisted extraction, chemical characteristics, and immunostimulatory activity of polysaccharides from Korean ginseng (<i>Panax ginseng</i> Meyer)

Song, Young-Ran,Sung, Su-Kyung,Jang, Mi,Lim, Tae-Gyu,Cho, Chang-Won,Han, Chun-Ji,Hong, Hee-Do Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.116 No.-

<P><B>Abstract</B></P> <P>In this study, enzyme-assisted extraction was used to isolate functional polysaccharides from Korean ginseng (<I>Panax ginseng</I> Meyer) and the physicochemical and biological properties of the extracted polysaccharides were investigated, comparing with those from traditional hot-water extraction (FGWP). In macrophages, their effects on cytokines production could be ordered as FGEP-CA ≥ FGEP-A > FGEP-C > FGWP, suggesting that FGEP-CA (combined cellulase- and α-amylase-extracted polysaccharide) is a potent immunostimulator. In addition, enzymatic digestion led to differences in the monosaccharide profile of the extract. FGWP mainly consisted of rhamnose, arabinose, galactose, galacturonic acid, and glucose in molar percentages of 1.8:10.1:9.2:17.8:60.6, whereas FGEP-CA was 3.2:11.4:16.5:22.3:45.8, respectively, suggesting that enzyme-assisted extraction of ginseng polysaccharides produces a higher proportion of pectin polysaccharides. The HPLC profile of FGEP-CA also showed lower and more heterogeneous molecular weights than FGWP did. In cyclophosphamide-induced immunosuppressed mice, FGEP-CA administration ameliorated decreased spleen and thymus indices (200 mg/kg), lymphocyte proliferation, natural killer cell activity, leukocyte counts, and the serum cytokines, interleukin-2, interleukin-6, and interferon-γ (100 and 200 mg/kg). These results suggest that enzyme-assisted extraction using cellulase and α-amylase is an effective method for the preparation of functional polysaccharides from fresh Korean ginseng, and FGEP-CA could be utilized as a potential immune-stimulatory agent.</P>

Efficacy of sentinel lymph node biopsy with radioisotope alone and the prediction of sentinel node status using PET-CT in breast cancer

Ran Song,Seong Uk Kwon,Dae Sung Yoon,In Eui Bae,In Seok Choi,Won Jun Choi,Sang Eok Lee,Ju Ik Moon,Nak Song Sung,Seung Jae Lee,Seung Jae Roh,Sung Gon Kim 대한종양외과학회 2021 Korean Journal of Clinical Oncology Vol.17 No.1

Purpose: Sentinel lymph node biopsy (SLNB) using both a radioactive isotope (RI) and blue dye is considered highly effective; however, there were limitations with the use of both agents in some hospitals, and blue dye has been shown to have some adverse effects. Additionally, preoperative prediction of sentinel lymph node (SLN) status using the maximum standardized uptake value (SUVmax) on positron emission tomography-computed tomography (PET-CT) can help avoid unnecessary axillary dissection or SLNB. Thus, we evaluated the efficacy and oncologic safety of SLNB using an RI alone in terms of long-term outcomes and determined the association between SLN metastasis and SUVmax of the primary tumor. Methods: This retrospective study was conducted at Konyang University Hospital between March 2011 and May 2018. Overall, 142 patients with breast cancer who underwent SLNB using an RI alone were enrolled. Data on identification and false-negative rates were collected. The SUVmax of primary tumors on PET-CT were analyzed for their association with SLN metastasis. Results: The identification and false-negative rates were 98.6% and 0%, respectively. There was no axillary local recurrence in patients with negative SLN findings. The correlation between the SUVmax of the primary tumor and SLN status was significant (r=0.249, P=0.005); the cutoff value for negative SLN metastasis was <2.15. Conclusion: The single agent method using an RI is not inferior to other methods and serves as a feasible option for SLNB. And the number of excised SLNs could be minimized when the SUVmax of primary tumor is extremely low.

Poster Session : Innate Immunity ; E2 of Hepatitis C Virus affects activation of NFkappaB

( Song Hee Lee ),( Ran Song ),( Mi Nam Lee ),( Chon Saeng Kim ),( Sung Key Jang ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Mutational analysis of mononucleotide repeats in dual specificity tyrosine phosphatase genes in gastric and colon carcinomas with microsatellite instability

SONG, SANG YONG,KANG, MI RAN,YOO, NAM JIN,LEE, SUG HYUNG Blackwell Publishing Ltd 2010 APMIS Vol.118 No.5

<P>Song SY, Kang MR, Yoo NJ, Lee SH. Mutational analysis of mononucleotide repeats in dual specificity tyrosine phosphatase genes in gastric and colon carcinomas with microsatellite instability. APMIS 2010; 118: 389–93.</P><P>Coordinated protein phosphorylation and dephosphorylation are crucial in the regulation of cell signaling, and disruption of the coordination is known to play important roles in cancer development. Recent reports revealed that classical protein tyrosine phosphatase (PTP)-encoded genes are somatically mutated in human colorectal cancer. However, data on dual specificity phosphatase (DPTP) gene mutations in human cancers are lacking. By analyzing a public genomic database, we found that five DPTP genes, <I>CDC14A</I>, <I>MTM1</I>, <I>MTMR3</I>, <I>SSH1</I>, and <I>SSH2</I>, have mononucleotide repeats in their coding DNA sequences. To see whether these genes are mutated in cancers with microsatellite instability (MSI), we analyzed the mononucleotide repeats in 26 gastric cancers (GC) with MSI (MSI-H), 12 GC with low MSI (MSI-L), 45 GC with stable MSI (MSS), 33 colorectal cancers (CRC) with MSI-H, 14 CRC with MSI-L, and 45 CRC with MSS by single-strand conformation polymorphism (SSCP). We found <I>CDC14A</I> and <I>MTMR3</I> mutations in five and one cancer (s), respectively. These mutations were detected in MSI-H cancers, but not in MSI-L or MSS cancers. The GC and CRC with MSI-H harbored the mutations in 15% and 6%, respectively. The <I>CDC14A</I> and <I>MTMR3</I> mutations detected in the GC and CRC were deletion or duplication mutations of one base in the nucleotide repeats that would result in premature stops of the amino acid syntheses. Our data show that frameshift mutations of DPTP genes in MSI-H cancers occur at moderate frequencies. The data suggested that alterations in the <I>CDC14A</I> and <I>MTMR3</I> genes may play a role in the development of GC and CRC with MSI-H by deregulating phosphatase functions possibly together with mutations of classical PTP genes.</P>