A study on left ventricular hypertrophy, QTc dispersion and hemodynamic differences in GXT according to excessive endurance exercise

( Piao Shun-zhe ),( Jin Zhong-xuan ),( Wang Guan ),( Zhao Ze-lin ),( Mu Yi-gang ),( Wen Jian-fei ),( Wang Xing ),( Il Su Kwon ),( Young Nam Cha ),( Young Ju Kim ) 대한운동사협회 2020 대한운동사협회 운동사대회자료집 Vol.2020 No.-

운동유발성 고혈압을 동반한 마라톤주자에서 운동부하검사상 좌심실 비대와 QT 분산 및 혈역학변화에 관한 연구

박순철 ( Shun Zhe Piao ),오재근 ( Jae Keun Oh ),김영주 ( Young Ju Kim ) 한국운동생리학회 2015 운동과학 Vol.24 No.2

METHODS: Total 48 healthy male marathoners (EIH, N=16, Normal Hypertension Group (NG), N=16, Control Group (CON), N=16) were participated in this research. Quinton stress test system was used to measure Left Ventricular Hypertrophy (LVH) and QT dispersion. Graded exercise test was executed by treadmill. RESULTS: LVH in EIH and Normal group (NG) was significantly greater than in Control group (CON) (p <.001, p <.01). Heart rate in EIH and NG during recovery phases (2 min, and 3 min) was more lower than in CON (p <.001, p <.01) and heart rate of EIH at recovery 5 min is lower than in CON (p <.05). The systolic blood pressure at rest, EIH was greater than in NG and CON (p <.05, p <.01). The systolic blood pressure of EIH at maximal, was higher than in the other groups and NG was also greater than in CON (p <.001, p <.001, p <.05). The systolic blood pressure of EIH at recovery 2 was higher than in the other groups and NG was also greater than in CON (p <.01, p <.001, p <.05). At recovery 3 min systolic blood pressure of EIH was higher than in the others and NG was significant greater than in CON (p <.05, p <.001, p <.01). Systolic blood pressure in EIH at recovery 5 min. was higher than in NG and CON (p <.01, p <.001). Cardiopulmonary function in EIH and NG were significantly greater compared to CON (p <.001). QT dispersion of EIH at rest was significantly higher than in CON (p <.05). There were no differences among the groups at recovery phases (2 min, 3 min, and 5 min). CONCLUSIONS: Systolic blood pressure and QT dispersion in EIH show significantly higher value compare to CON at rest. These results indicate that middle-aged male marathoners with EIH have higher cardiovascular risk factors than CON. Further researches will be necessary for identifying an effect of continuous high intensity exercise on cardiovascular system.

Synergistic Effects of Leflunomide and Benazepril in Streptozotocin-Induced Diabetic Nephropathy

Jin, Hua,Piao, Shang Guo,Jin, Ji Zhe,Jin, Ying Shun,Cui, Zhen Hua,Jin, Hai Feng,Zheng, Hai Lan,Li, Jin Ji,Jiang, Yu Ji,Yang, Chul Woo,Li, Can S.Karger 2014 The Nephron Journals Vol.126 No.3

<P>Abstract</P><P><B><I>Background:</I></B> Leflunomide (LEF) and benazepril have renoprotective effects on diabetic nephropathy (DN) through their anti-inflammatory and anti-fibrotic activities. This study investigated whether combined treatment using LEF and benazepril affords superior protection compared with the respective monotherapies. <B><I>Methods:</I></B> Diabetes was induced with streptozotocin (STZ, 65 mg/kg) by intraperitoneal injection in male Wistar rats. Two weeks after STZ injection, diabetic rats were treated daily for 12 weeks with LEF (10 mg/kg), benazepril (10 mg/kg), or a combination of both. Basic parameters (body weight, fasting blood glucose level, and 24 h urinary protein excretion), histopathology, inflammatory [inflammatory cell infiltration (ED-1), monocyte chemoattractant protein-1 (MCP-1), and Toll-like receptor-2 (TLR-2)] and glomerulosclerotic factors [transforming growth factor-β<SUB>1</SUB> (TGF-β<SUB>1</SUB>) and connective tissue growth factor (CTGF)], and oxidative stress (8-hydroxy-2'-deoxyguanosine, 8-OHdG) were studied. <B><I>Results:</I></B> Benazepril or LEF treatment significantly prevented body weight loss and 24 h urinary protein excretion induced by diabetes; combined treatment with LEF and benazepril further improved these parameters compared with giving each drug alone (all p < 0.01). Increased expression of inflammatory (MCP-1 and TLR-2) and glomerulosclerotic (TGF-β<SUB>1</SUB> and CTGF) factors in diabetic rat kidney was reduced by treatment with either LEF or benazepril and was further reduced by the combined administration of the two drugs (p < 0.01). These effects were accompanied by suppression of urinary 8-OHdG excretion. There was no significant between-group difference in blood glucose level. <B><I>Conclusions:</I></B> LEF treatment lessens DN, and combined treatment with LEF and benazepril provides synergistic effects in preventing DN.</P><P>© 2014 S. Karger AG, Basel</P>

2,5-Hexanedione induces apoptosis via a mitochondriamediated pathway in PC12 cells

Yuan Qi,Shuang-yue Li,Feng-yuan Piao,Zhe-min Wang,Ruo-lin Chen,Shuang Liu,Jing-shun Shen 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.1

2,5-Hexanedione (HD) is the main active metabolite of n-hexane and mediates the neurotoxicity of the parent compound. Studies suggested that apoptosis involved in HD neurotoxicity. However, the mechanism of HD-induced neuronal apoptosis remains unknown. To explore its underlying mechanism, we treated PC12 cells with 5, 10 and 20 mM HD for 24 h, respectively. We found that HD induced apoptotic death in PC12 cells in a dose-dependent manner. Moreover, HD down-regulated Bcl-2 expression, up-regulated Bax expression and Bax/Bcl-2 ratio, promoted the disruption of mitochondrial transmembrane potential, induced the release of cytochrome c from mitochondria, and increased the activity of caspase-3 in PC12 cells, which were all the key regulators of intrinsic apoptotic pathway. These results indicate that HD induces apoptosis via a mitochondria- mediated pathway in PC12 cells.

622km 울트라 마라톤 주자의 운동중독 성향 분석

오재근 ( Jae-keun Oh ),박순철 ( Shun-zhe Piao ),박다운 ( Da-woon Park ),송기재 ( Ki-jae Song ) 한국체육대학교 체육과학연구소 2017 스포츠사이언스 Vol.34 No.2

본 연구는 대한울트라마라톤연맹 소속으로 622km국토종단대회에 참여한 울트라마라톤 주자들을 대상으로 연령, 운동경력, 운동빈도, 운동시간에 따라 금단, 갈등, 애착, 내성, 강박 등 다섯 가지 하위요인으로 구성된 운동중독 척도를 이용해 설문기법 연구를 진행하였다. 운동중독의 성향을 확인하기 위해 강신욱(2009)이 개발한 5점 척도의 총18문항 한국형 운동중독 척도를 활용하였으며, 대회일 기준으로 최근 2년 이내에 대한울트라마라톤연맹(KUMF) 공인 200km이상을 완주하였으며, 종단대회인 622km 울트라마라톤 대회에 참여하여 자발적 참여의사를 밝힌 주자 50명에 한하여 설문을 진행하였다. 또한 정확한 설문조사를 위해 본 연구분야에 관심이 있으며 설문의 내용을 숙지하고 있는 대학원생 3명이 보조연구원으로 참여하였다. 설문결과는 Window 용SPSS/PC 21.0 통계 프로그램을 이용하여 하위요인별 운동중독 성향을 파악하기 위해 연령, 운동빈도, 운동경력, 운동시간 변인별각 두 그룹으로 설정하여 T-test를 통해 자료처리를 실시한 결과는 다음과 같았다. 1. 연령에 따른 금단, 갈등, 애착, 내성, 강박 모든 요인에는 유의한 차이가 나타나지 않았다. 2. 운동경력에 따른 금단, 갈등, 애착, 내성, 강박 모든 요인에는 유의한 차이가 나타나지 않았다. 3. 운동빈도에 따른 금단, 갈등, 내성, 강박 4가지 요인에는 유의한 차이가 나타나지 않았으나 애착요인의 경우 주간 운동참가 횟수가 4회 미만 참가와 4회 이상 참가자에서 유의한 차이가 나타났다(p=.011). 4. 운동시간에 따른 금단, 갈등, 애착, 내성, 강박 모든 요인에는 유의한 차이가 나타나지 않았다. The study was to observed the exercise addiction and the psychological condition of participants(N=50) in the 622 marathon event, which belonged to Korea Ultra Marathon Federation. The questionnaire consists of withdrawal, conflict, attachment, tolerance, and compulsiveness and total 18 questions. The survey results were divided into age, experience, frequency, and time, and T-tested using by windows SPSS PC 21.0 statistics analysis program.. The results are as follows. 1. There was no significant difference in the amount of withdrawal, conflict, attachment, tolerance and compulsiveness and all factors caused by age. 2. There was no significant difference in the amount of withdrawal, conflict, attachment, tolerance and compulsiveness and all factors caused by athletic career. 3. There were no significant differences in the amount of withdrawal, conflict, tolerance and compulsiveness caused by exercise frequency. However, the attachment of participated in the attendance of four or more, and the difference in the number of participants was greater than 4 times(p =.011). 4. There was no significant difference in the amount of exclusion, conflict, attachment, tolerance and compulsiveness and all factors caused by exercise time.

홍삼과 갈조류 혼합 음료 섭취가 여성 노인의 체력 변인에 미치는 영향

권일수 ( Kwon Il-su ),박득수 ( Park Deuk-su ),박순철 ( Piao Shun-zhe ),오재근 ( Oh Jae-keun ) 한국체육대학교 체육과학연구소 2020 스포츠사이언스 Vol.38 No.1

본 연구의 목적은 여성 노인을 대상으로 1회성 홍삼과 갈조류 혼합음료 섭취 유무가 체력 변인에 어떠한 효과가 있는지 알아보는데 있다. 이를 위해 서울 G구 노인종합복지관 등록 노인 10명을 대상으로 1주일의 wash-out 기간을 두고 위약과 홍삼 갈조류 음료를 섭취하는 이중맹검실험법을 실시하였다. 신장 및 체질량지수 측정 후, 상. 하지 유연성, 근력, 민첩성, 평형성, 하지 근지구력, 심폐지구력 및 협응력을 측정하였다. 측정은 각 음료 섭취 30분 후 실시하였다. 섭취물에 따른 집단 간 차이를 검증하기 위해 Wilcoxon rank sum test를 실시하였으며, 이때 모든 통계적 유의 수준은 α=.05로 설정하였다. 섭취물에 따른 하지 유연성(p=.021), 우측 상지 유연성(p=.023), 평형성(p=.023), 심폐지구력(p=.015)은 집단 간 통계적으로 유의한 차이가 나타났으며, 근력, 민첩성, 하지 근지구력, 협응력에 대해서는 유의한 차이가 나타나지 않았다. 따라서, 홍삼과 갈조류 섭취는 여성 노인 체력 변인 중 유연성과 평형성, 심폐지구력 변인에 유의한 차이를 나타냄으로 긍정적인 효과를 보았으며, 추가적인 연구를 통하여 더 많은 대상자, 성별, 장기간 섭취에 따른 연구가 필요할 것으로 판단된다. The purpose of this study is to investigate the effects of red ginseng and brown algae mixed drink intake on fitness in elderly women. The participants were 10 persons for red ginseng/brown algae mixed drink and placebo. After 1week of wash out period from the placebo, the experiment was conducted for red ginseng/brown algae intake by double blind test. The flexibility of the lower limb, muscle strength, agility, balance, muscle endurance, cardiopulmonary endurance, coordination ability were measured. In order to verify the differences, an independent variable t-test was conducted. All statistical significance levels were set at α= .05. The lower limb flexibility (p = .021), right upper limb flexibility (p = .023), balance(p = .023), and cardiorespiratory endurance (p = .015) showed significant differences between groups. There was no significant difference in agility, muscle endurance, and coordination. In conclusion, red ginseng and brown algae intake showed a positive effect on the variables of flexibility, balance, and cardiorespiratory endurance among the elderly women. Further research might be investigated for the wide range of participants, other gender, longer term of intake to secure the effects of the red ginseng and brown algae mixed drinks.

냉온 자극에 따른 슬관절의 고유수용성감각 능력의 차이

오재근 ( Jae-keun Oh ),강성우 ( Sung-woo Kang ),박순철 ( Shun-zhe Piao ) 한국체육대학교 체육과학연구소 2016 스포츠사이언스 Vol.33 No.2

본 연구는 관절의 온도 변화가 관절의 고유수용성감각 수용기에 미치는 영향을 알아보고, 올바른 냉처치와 온열처치방법을 위한 기초자료를 제시하는데 그 목적이 있다. 연구대상자는 신체가 건강한 남자16명(24.43±3.11 yr, 172.26±5.13 cm, 68.86±7.36 kg, 14.80±4.52 %BF)을 무작위로 선정하였다. 냉처치는 1kg의 부서진 아이스를 이용한 아이스 팩(ice pack), 온열처치는 핫 팩(hot pack)을 이용하여 각각 슬관절에 15분간 처치하였다. 슬관절 능동ㆍ수동적 고유수용성감각 기능 측정은 등속성 장비 CSMi(HUMAC, U.S.A)를 이용하였고, 가동범위는 0°~90°로 설정하였으며, 목표 각 45°와 15°에서 5초 동안 움직임을 정지시켜 피험자에게 목표 각을 인지시킨 후 재현하도록 하였다. 정적균형 측정은 눈감고 외발서기로 측정하였으며, 동적균형 측정은 Biodex Balance System(Biodex, U.S.A)를 이용하여 외발서기로 Stage-12, Stage-6, Stage-1 세 단계로 나누어 측정하였다. 처치방법에 따른 차이를 비교하기 위해 반복측정 변량분석(repeated measure ANOVA)을 실시하였으며, 모든 통계적 유의 수준은 α=.05로 설정하였다. Stage-1 종합균형능력에서 온열처치(3.88±2.03)가 무처치(5.28±3.06)에 비해 유의하게 낮은 것으로 나타났다(p<.05). Stage-1 전ㆍ후 균형능력에서 냉처치(3.05±1.52)와 온열처치(2.71±1.43)가각각 무처치(4.23±2.52) 보다 유의하게 낮은 것으로 나타났다(p<.05, p<.01). Stage-1 내ㆍ외측 균형능력에서 냉처치(2.32 ±1.09)와 온열처치(2.26±1.22)가 각각 무처치(2.92±1.48) 보다 유의하게 낮은 것으로 나타났다(p<.05, p<.05). 이상의 결과로 볼 때 냉처치와 온열처치는 고유수용성감각 수용기에 영향을 미치는 것으로 사료되며, 향후 보다 많은 피험자들을 대상을 추가적인 연구를 하여 냉처치와 온열처치가 고유수용성감각에 미치는 영향을 조사해야 할 것으로 사료된다. The purpose of this study is to investigate the effects of joint temperature change to the proprioceptive capabilities and to provide the baseline data for cro-therapy and thermo therapy. 16 healthy males were randomly selected (24.43±3.11 yr, 172.26±5.13 cm, 68.86±7.36 kg, 14.80±4.52 %BF). Cryotherapy with 1kg of broken ice bag, thermotherapy with hot pack to knee joint applied for 15 minutes respectively. Knee joint active and passive proprioceptive sensory measurement was performed using an isokinetic equipment CSMi (HUMAC , USA) , the movable range was set to 0° ~ 90° , the target to stop the movement for 5 second s at each 45° and 15° to recognize the subject to reproduce after each goal . Static balance measurements were measured with eyes closed and one leg standing , dynamic balance measurement was measured with Biodex Balance System (Biodex, U.S.A) by dividing the Stage-12, Stage-6, Stage-1 in three steps. All data of this research were analyzed by SPSS/PC ver 21.0 and. repeated measure ANOVA was used to compare the difference between the treatment methods. Post-hoc was conducted by LSD the significance level score was set as α=.05. The results through this process are as followed. Stage-1 overall stability in thermotherapy(3.88±2.03) was significantly lower than in no therapy(5.28±3.06)(p<.05). Stage-1 anterior and posterior stability in cryotherapy(3.05±1.52) and thermotherapy(2.71±1.43) were significantly lower than in no therapy(4.23±2.52)(p<.05, p<.01). Stage-1 medial and lateral stability in cryotherapy(2.32±1.09) and thermotherapy(2.26±1.22) were significantly lower than in no therapy(2.92±1.48)(p<.05, p<.05). According to the result of this study, cryotherapy and thermotherapy are considered to affect the proprioceptive sense. In the future study, experiments against additional number of subjects required to be conducted to ensure the effects of cryotherapy and thermotherapy.

갈조류 추출물 섭취가 사이클 선수의 운동피로회복에 미치는 영향

오재근 ( Oh Jae-keun ),이용우 ( Lee Yong-woo ),김일호 ( Kim Il-ho ),박다운 ( Park Da-woon ),박순철 ( Piao Shun-zhe ) 한국체육대학교 체육과학연구소 2016 스포츠사이언스 Vol.34 No.1

본 연구는 갈조류 추출물 섭취가 엘리트 사이클 선수들의 유산소성 능력 및 운동피로와 관련이 있는 혈액인자에 어떠한 영향을 미치는지에 대해 구명하는데 목적이 있다. 이를 위해 K 대학교 엘리트 사이클 선수 EXP(실험) 집단 6명, PLA(위약) 집단 7명 총 13명이 피험자로 연구에 참여하였다. 갈조류 추출물과 위약은 EXP집단과 PLA집단 모두 운동부하검사 15분 전 섭취하였다. 운동부하검사는 사이클 실내훈련용 자전거인 와트바이크 프로(Wattbike pro., UK)를 이용하여 측정하였고, 심박수는 전자 심박수측정계(Pola S810, Finland)를 이용하여 기록하였다. 운동피로관련 혈액변인은 Glucose, Lactate acid, Ammonia, LDH를 운동 전, 직후, 회복기 15분, 30분, 60분 총 5회 채취하여 분석하였다. 섭취물에 따른 차이를 비교하기 위해 집단 간 유산소성 능력의 차이는 Independent t-test, 집단 간 및 집단 내 심박수, 혈액변인은 Repeated measure ANOVA를 실시하였고, 사후검증은 LSD(least signigicant difference)를 실시하였으며, 이때 모든 통계적 유의 수준은 α=.05로 설정하였다. 섭취물에 따른 VO2max와 Lactate threshold는 집단 간 시기와 시기 * 집단에서 차이가 나타나지 않았다 (p=.737, p=.458). 집단 간 안정시, 회복기 심박수와 운동 중 심박수는 시기에서 유의한 차이가 있었으나(p=.001) 시기 * 집단에는 유의한 차이가 나타나지 않았다(p=.440). Lactate acid, Ammonia, LDH는 시기에는 유의한 차이가 있었으나(p=.001) 시기 * 집단에는 유의한 차이가 나타나지 않았다(p=.799, p=.870, p=.150). Glucose는 시기와 시기 * 집단 모두 유의한 차이가 있는 것으로 나타났다(p=.001, p=.03). 이상의 결과를 미루어 볼 때, 갈조류 추출물 섭취는 운동피로관련 혈액인자에 긍정적인 영향을 미치는 것으로 사료되며, 고강도의 사이클 경기 및 훈련 후 회복능력과 경기력 향상에 긍정적인 처치 방법으로 제시할 수 있을 것이라고 사료된다. AThis study aims to identify the effects of eclonia cava extract intake to the blood factors that are associated with aerobic capaciThis study aims to identify the effects of eclonia cava extract intake to the blood factors that are associated with aerobic capacity and exercise fatigue of the elite cyclists. To conduct this study, 6 persons of EXP group elite cyclists and 7 persons of PLA group elite cyclists, total 13 elite cyclists from K university were selected. Both eclonia cava extract and placebo groups consumed eclonia cava extract and placebo respectively before 15 minutes before exercise stress test. Exercise stress testing was measured using watt cycle bike pro (Wattbike pro, UK) which is indoor cycle training bike, heart rate was recorded using an electronic heart rate meter (Pola S810, Finland). For exercise fatigue-related factors, Glucose, Lactate acid, Ammonia and LDH were collected and analyzed before exercise, immediately after exercise, 15minutes, 30 minutes, 60 minutes of recovery period total 5 times. To analyze the differences of aerobic capacity between groups according to ingesta, independent t-test was conducted, for heart rate and blood factors , repeated measure ANOVA was performed and LSD(least signigicant difference) was conducted for post verification. All the statistical significance level was set at α=.05. Lactate threshold and VO<sub>2max<sub> differences according to ingesta was not shown between the groups and time * group (p=.737, p=.458). At recovery, there was a significant difference in heart rate at rest and heart rate during exercise between groups(p=.001), but the time * group (p=.440) did not show significant difference. Lactate acid, Ammonia, LDH showed a significant difference in times(p=.001), but no significant difference found in time * group (p=.799, p=.870, p=.150). Glucose is found to have significant differences time and time * group (p=.001, p=.03). Considering from the above results, intake of eclonia cava extract is considered to have a positive impact on the relevant blood factors exercise fatigue, and can be suggested as positive aid measures after a high-intensity cycle match and training resilience and performance improvement.