Oleoylethanolamide induces eosinophilic airway inflammation in bronchial asthma

Kwon Eun-Kyung,최영우,Yoon Il-Hee,Won Ha-Kyeong,심소윤,Lee Hee-Ra,Kim Hyoung Su,예영민,신유섭,박해심,Ban Ga-Young 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Asthma is a chronic eosinophilic inflammatory disease with an increasing prevalence worldwide. Endocannabinoids are known to have immunomodulatory biological effects. However, the contribution of oleoylethanolamide (OEA) to airway inflammation remains to be elucidated. To investigate the effect of OEA, the expression of proinflammatory cytokines was measured by RT-qPCR and ELISA in airway epithelial (A549) cells. The numbers of airway inflammatory cells and cytokine levels in bronchoalveolar lavage fluid, airway hyperresponsiveness, and type 2 innate lymphoid cells (ILC2s) were examined in BALB/c mice after 4 days of OEA treatment. Furthermore, eosinophil activation after OEA treatment was evaluated by measuring cellular CD69 levels in eosinophils from human peripheral eosinophils using flow cytometry. OEA induced type 2 inflammatory responses in vitro and in vivo. OEA increased the levels of proinflammatory cytokines, such as IL-6, IL-8, and IL-33, in A549 cells. In addition, it also induced eosinophilic inflammation, the production of IL-4, IL-5, IL-13, and IL-33 in bronchoalveolar lavage fluid, and airway hyperresponsiveness. OEA increased the numbers of IL-5- or IL-13-producing ILC2s in a mouse model. Finally, we confirmed that OEA increased CD69 expression (an eosinophil activation marker) on purified eosinophils from patients with asthma compared to those from healthy controls. OEA may play a role in the pathogenesis of asthma by activating ILC2s and eosinophils.

Effects of <i>Ecklonia cava</i> ethanolic extracts on airway hyperresponsiveness and inflammation in a murine asthma model: Role of suppressor of cytokine signaling

Kim, Se-Kwon,Lee, Da-Young,Jung, Won-Kyo,Kim, Ji-Hye,Choi, Inhak,Park, Sae-Gwang,Seo, Su-Kil,Lee, Soo-Woong,Lee, Chang Min,Yea, Sung Su,Choi, Yung Hyun,Choi, Il-Whan Elsevier 2008 BIOMEDICINE AND PHARMACOTHERAPY Vol.62 No.5

<P><B>Abstract</B></P><P><I>Ecklonia cava</I> (EC) is a brown alga that evidences radical scavenging activity, bactericidal activity, tyrosinase inhibitory activity, and protease inhibitory activity. However, its anti-allergic effects remain poorly understood. In the current study, we attempted to determine whether pretreatment with EC induces a significant inhibition of asthmatic reactions in a mouse asthma model. Mice sensitized and challenged with ovalbumin (OVA) evidenced typical asthmatic reactions, as follows: an increase in the number of eosinophils in bronchoalveolar lavage fluid; a marked influx of inflammatory cells into the lung around blood vessels and airways, and airway luminal narrowing; the development of airway hyperresponsiveness; the detection of tumor necrosis factor-alpha (TNF-α) and Th2 cytokines, including IL-4 and IL-5 in the bronchoalveolar lavage (BAL) fluid; and the detection of allergen-specific immunoglobulin E (IgE) in the serum. However, the administration of EC extract prior to the final airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. We also demonstrated that EC extracts treatment resulted in significant reductions on matrix metalloproteinase-9 (MMP-9) and Suppressor of cytokine signaling-3 (SOCS-3) expression and a reduction in the increased eosinophil peroxidase (EPO) activity. The treatment of animals with EC extracts resulted in a significant reduction in the concentrations of the Th2 cytokine (IL-4 and IL-5) in the airways, without any concomitant increase in the concentration of Th1 cytokines. These findings indicate that EC extracts may prove useful as an adjuvant therapy for allergic airway reactions via the inhibition of the Th2 response. Accordingly, this study may provide evidence that EC extract performs a critical function in the amelioration of the pathogenetic process of asthma in mice.</P>

과학적 규칙성 지식의 생성 과정 : 경향성 지식의 생성을 중심으로 Focused on the Generation of Scientific Pattern-Knowledge

권용주,박윤복,정진수,양일호 한국초등과학교육학회 2004 초등과학교육 Vol.23 No.1

The purpose of this study was to suggest a grounded theory on the process of undergraduate students' generating pattern-knowledge about scientific episodes. The pattern-discovery tasks were administered to seven college students majoring in elementary education. The present study found that college students show five types of procedural knowledge represented in the process of pattern-discovery, such a s element, elementary variation, relative prior knowledge, predictive-pattern, and final pattern-knowledge. Furthermore, subjects used seven types of thinking ways, such as recognizing objects, recalling knowledges, searching elementary variation, predictive-pattern discovery, confirming a predictive-pattern, combining patterns, and selecting a pattern. In addition, pattern-discovering process involves a systemic process of element, elementary variation, relative prior knowledge, generating and confirming predictive-pattern, and selecting final pattern-knowledge. The processes were shown the abductive and deductive reasoning as well as inductive reasoning. This study also discussed the implications of these findings for teaching and evaluating in science education.

급성 A형 간염에 병발한 급성신부전 2 례

홍수민;박경식;박일권;손형래;구호석;고행일 인제대학교 2011 仁濟醫學 Vol.32 No.-

Hepatitis A is a mild, self-limiting disease of the liver, critical complications of which, such as acute kidney injury, are rare. Two cases of patients with acute hepatitis A who had an acute renal failure complication are reported herein. The first case is that of a 30-year-old man who showed up at the author’s hospital complaining of fever and myalgia. His laboratory tests upon his admission showed 9,520 lU/L of aspartate transaminase, 5,600 lU/L of alanine transaminase, 31.9 mg/dL of blood urea nitrogen, and 5.5 mg/dL of creatinine, and he was positive for the Anti-HAV IgM antibody. Upon his admission, supportive treatment was started, which included fluid therapy. The laboratory tests on the next day showed 53 mg/dL of blood urea nitrogen, 9.9 mg/dL of creatinine, and lower urine output per day. Renal replacement therapy was performed only once. A renal biopsy was performed and the result was Acute Tubular Necrosis (ATN). The patient recovered from acute renal failure without sequela. In the second case, a 35-year-old man complained of fever and abdominal pain. His laboratory tests upon his admission showed 8,570 lU/L of aspartate transaminase, 4,510 lU/L of alanine transaminase, 16 mg/dL of blood urea nitrogen, and 1.19 mg/dL of creatinine, and he was positive for the Anti-HAV IgM antibody. He experienced acute renal failure and recovered after two-time renal replacement therapy. The result of the renal biopsy was Acute Tubular Necrosis and C1q nephropathy.

Cilostazol의 항혈소판 응집작용과 sodium citrate의 농도에 따른 혈소판 응집율에 대한 연구

우수경,김영리,권광일 충남대학교 약학대학 의약품개발연구소 2000 藥學論文集 Vol.16 No.-

Cilostazol, 6- 〔4- (1-cyclohexyl- 1H-tetrazol -5-yl) butoxy〕 - 3, 4-dihydro- 2(1H)- quinolinone, is an antiplatelet/antithrombotic agent that has been used for treatment of chronic peripheral arterial occlusion. Cilostazol was studied for its inhibitory effects on platelet aggregation in vitro in rabbit plasma and in human plasma, and also for its antiplatelet effects ex vivo in rabbit after single oral administration. At time t=0, the rabbits were given a single oral dose of 14 ㎎/㎏ cilostazol. At 1, 2, 4, 8, 12, and 24 hours after drug administration, blood samples were taken for measurements of antiplatelet aggregation effect. Before the study of antiplatelet effects of cilostazol in rabbits, the effect of sodium citrate on platelet aggregation in various concentrations was studied to determine the optimal treatment. Cilostazol produced a potent inhibitory effect on platelet aggregation induced by adenosine diphosphate (ADP) in vitro. The maximum effect on antiplatelet aggregation was 14.4% and was appeared at 1 hour after drug administration. The optimum blood/anticoagulant (3.8% sodium citrate) ratio was 9 to 1, and 16 to 1 in human plasma and in rabbit plasma, respectively.

국내에서 분리된 Esherichia Coli 와 Klebsiella pneumoniae의 CTX-M형 Extended-Spectrum β-Lactamase 생성 현황

배일권,우건조,정석훈,박광옥,조병규,김둘만,권수봉,김현주,강현경 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

국내외 현황 파악을 통한 국내 소아용 의약품의 안전성.유효성 평가제도 개선 방안

이병요,이수진,권광일 충남대학교 약학대학 의약품개발연구소 2013 藥學論文集 Vol.28 No.-

Recently it has been noticed that pharmacokinetics / Pharmacodynamic (PK/PD) properties of drug in the pediatric population are different from those in adults. Clinical trials that considering age-specific properties and characteristics should be performed and safety and efficacy are established based on the clinical trials. FDA and EMEAs exists in developed countries especially America and Europe due to which there are proper guidelines for pediatric patients. However, in Korea, there doesn’t exist any guidelines and the study is focused regarding it. This study aimed to analyze the current state of safety and efficacy assessment of pediatric drug by foreingn drug regulatory agencies and suggest the regulatory methods for Korea based on FDA and EMEA guidelines and regulatory. MEDLINE and foreign drug regulatory agency database were extensively searched to obtain scientific research articles, guidance, regulations and pediatric drug review reports on foreign pediatric drug evaluation system.

황련해독탕 및 귀비탕의 발효 전후 혈소판 응집 억제 효과에 대한 비교 연구

송병정,이수진,권광일 충남대학교 약학대학 의약품개발연구소 2012 藥學論文集 Vol.27 No.-

Recently interest of fermented oriental medicines is increasing for the purpose of disease prevention. Hwangryunghaedok-tang (HR) and Guibi-tang (GB) are widely used oriental medicines in Korea. HR and GB are well known for anti-inflammatory and anti-stress effect. Glycosides in herbal medicines are transformed to aglycone via fermentation. The fermented medicines are absorbed more and have less individual variation. In this study, HR and GB were compared before and after fermentation by anti-platelet aggregation effect. HR and GB were fermented by various lactobacillus species. The anti-platelet aggregation effect of HR and GB were measured using aggregometer by optical method. Collagen (4 μg/mL) was used as a inducer of platelet aggregation. After measuring anti-platelet aggregation effect, we analysed the data using WinnonlinⓇ program to fit simple Emax model. Then Emax and EC50 values were estimated. In results, GB had little antiplatelet effects. Emax of 5 GB samples (127, 164, 402, 442, 3163) were estimated near zero and others (129, 144, 166, 693, 744) had high EC50 values (> 1500 μg/mL). In contrast with GB, HR had antiplatelet effects. HR which was fermented by Lactobacillus casei and Lactobacillus plantarum (HR-127,HR-144) were most effective sample. Emax and EC50 of HR-127 and HR-144 was 99.99%, 174.73 μg/mL and 100.00% 160.48 μg/mL, respectively. These results indicated that Guibi-tang was inappropriate as anti-platelet aggregation agent and Hwangryunhaedok-tang would be more effective after fermentation as anti-platelet aggregation agent, warranting further study.

부비동 내시경수술 후 비음도 변화

동헌종,김선일,권중근,윤영선,강수미,추광철 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

지리산 고로쇠나무 군락의 토양환경과 식생구조

문현식,노일,권수덕 경상대학교 농업생명과학연구원 2004 농업생명과학연구 Vol.38 No.2

본 연구는 경남 하동 지리산 지역 고로쇠나무 군락의 토양환경과 식생구조를 분석하여 고로쇠나무의 경영과 분포지에 대한 기초적인 정보를 제공하기 위하여 수행되었다. 고로쇠나무 군락지의 토양 pH와 유기물함량은 각각 4.66, 14.2%로 측정되었으며, 전질소함량은 0.71%, 유효인산 함량은 23.9ppm으로 나타났다. 층위별 중요치는 상층에서 사람주나무가 55.3으로 가장 높았고 다음으로 고로쇠나무(3.29), 비목(29.0), 서나무(26.0), 중층에서는 사람주나무(65.6), 생강나무(34.7), 때죽나무(23.3), 하층에서는 생강나무(15.5)와 덜꿩나무(13.3)의 중요치가 높게 나타났다. 층위별 종다양도는 상층1.123, 중층 1.173, 하층 1.519이며 균재도는 상층0.753, 중층 0.802. 하층 0.984로 나타나 본 조사지역의 고로쇠나무 군락은 상·중층에 비해 하층의 출현식물의 분포가 보다 균일한 경향임을 알 수 있었다. The present study was conducted to investigate the soil condition and vegetation structure of Acer mono communities for providing the information on management and distribution in Mt. Jiri national park. Soil pH of A. mono communities was 4.66. The -contents of soil organic matter, total N and available P₂O_(5) were 14.2%, 0.71% and 23.9ppm, respectively. According to the importance value by layer on the A. mono communities, Sapium japonicum was the highest as 55.3, and followed in order of A. mono 32.9, Lindera erythrocarpa 29.0 and Carpinus laxiflora 26.0 in upper layer, and S. japonicum 65.6, L. obtusiloba 34.7 and Styrax japonica 23.3 in middle layer, and L. obtusiloba 15.5 and Viburnum erosum 13.3 in lower layer. Species diversity and evenness were 1.123, 0.753 in upper, and 1.173 and 0.802 in middle, and 1.519 and 0.984 in lower layer, respectively. It showed that distribution of appeared species of A. mono communities in Mt. Jiri was more uniform tendency in lower layer than upper and middle layer.