Purification and Characterization of an Extracellular Protease from Bacillus pumilus CN8

Mei-Hu Ma,Yong-Guo Jin,Hao-Li Li,Jun Wang,Ha-Na Kim,오덕환 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.1

The protease produced by a Bacillus pumilus CN8 strain was purified by DEAE-Cellulose-52 ion exchange. It has a molecular weight of approximately 96,920 Dalton. In the present study, this protease showed strong activity over a broad range of pH (6.5-9.5) and temperature from 40℃ to 60℃, and the protease performed the maximal activity at pH 7.3 at 42℃. The effect of metal ions on protease activity showed that K+ could slightly increase the protease activity, and other ions such as Zn²+, Fe²+, Na+, Ca²+, Mg²+ had no significant activation or inhibition to the protease (P > 0.05), and the more important is that Cu²+, Mn²+, Sn²+, Cd²+ had a strong inhibitory effect on the protease activity.

이뇨제를 사용한 급성 신손상 환자에서 FEUrea의 진단적 유용성

임대훈 ( Dae Hun Lim ),정지민 ( Ji Min Jeong ),오슬현 ( Seul Hyun Oh ),이형철 ( Hyung Chul Lee ),최준석 ( Joon Suk Choi ),김민지 ( Min Jee Kim ),박정우 ( Jeong Woo Park ),배은희 ( Eun Hui Bae ),마성권 ( Seong Kwon Ma ),김남호 ( Na 대한신장학회 2009 Kidney Research and Clinical Practice Vol.28 No.3

목적: 임상적으로 FENa가 일과성 신손상 (T-AKI)과 지속성 신손상 (P-AKI)을 감별하는 데 많이 사용되지만 이뇨제를 사용한 환자에서 FENa는 유효 혈류량 결핍 상태에서도 증가하게 되어 진단적 정확성이 떨어진다고 알려져 있다. 본 연구에서는 이뇨제 투여상황에서 FE(Na)와 비교하여 FE(Urea)의 진단적 유용성에 대하여 알아보고자 하였다. 방법: 107명의 급성 신손상 환자를 임상적 특성에 따라 일과성과 지속성 신손상 군으로 나누고 이를 다시 이뇨제 투여 유무에 따라 재분류하였다. ROC 곡선에 따라 계산된 cutoff value에 따라서 일과성 신손상을 정의하였고 이뇨제 투여로 인한 cutoff value의 변화에 따른 민감도와 특이도를 비교하였다. 결과: AKI가 발생한 107명의 모든 환자중 검사 이전에 경험적으로 이뇨제를 사용한 경우가 67명으로 63%를 보였고, 일과성 신손상군의 경우는 52명 중 27명으로 52%를, 지속성 신손상군의 경우는 55명 중 45명으로 73%를 보였다. ROC curve에 따라 cutoff value를 FE(Na)≤1.5 FE(Urea)≤30으로 하였을 때 T-AKI를 진단하는 데 사용된 두 값의 민감도와 특이도는 모든 환자군에서 FE(Na)가 81%, 98%를, FE(Urea) 가 94%, 82%를 보였다. 이뇨제 비투여군에서는 FE(Na)가 96 %, 100%를, FEUrea가 92%, 87%를 보였으며, 이뇨제 투여군에서는 FE(Na)가 63%, 98%를, FE(Urea)가 96%, 83%를 보였다. 결론: 이뇨제를 사용한 경우 P-AKI를 진단하는데 FE(Urea)도 FE(Na) 정도의 진단적 유용성을 가진다. Purpose: Although fractional excretion of sodium (FE(Na)) has been used to distinguish transient-acute kidney injury (T-AKI) from persistent-AKI (P-AKI), the availability of FE(Na) in the diagnosis of T-AKI is reported low in patients with diuretics use. We compared the diagnostic performance of fractional excretion of urea (FE(Urea)) with that of FE(Na) in patients with diuretics use. Methods: One hundred seven AKI patients were classified as having T-AKIor P-AKI according to the clinical context. Each group was again subdivided according to exposure to diuretics. According to the cut off value generated by receiver operating characteristic (ROC) curves, sensitivity and specificity of FE(Na) and FE(Urea) were compared with each other. Results: The numbers of patients administered with diuretics were 67 out of total 107 AKI patients (63%), 27 out of 52 (52%) of T-AKI patients, and 40 out of total (65) 55 (73%) of P-AKI patients. When the cutoff value of T-AKI was defined as FE(Na) ≤1.5 and FE(Urea) ≤30 according to the ROC curves, sensitivity and specificity of FE(Na) were 96% and 100% in non-diuretics group, and 63% and 98% in diuretics group, respectively. Sensitivity and specificity of FE(Urea) were 92% and 87% in non-diuretics group, and 96% and 83% in diuretics group, respectively. Conclusion: FE(Urea) is as good as FE(Na) at distinguishing T-AKI from P-AKI in patients administered with diuretics.

Biocatalytic Synthesis of Biodiesel by Methanolysis of High Oleic Sunflower Oil in Supercritical Carbon Dioxide

Na Ma,Seung In Hong,Sung Won Yoon,Da Som No,Chong-Tai Kim,In-Hwan Kim 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10

Enrichment of DHA by Esterification of Tuna Oil Fatty Acid and Ethanol in Packed Bed Reactor

Na Ma,Seung In Hong,Chong-Tai Kim,Ramiro Baeza Jimenez,In-Hwan Kim 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10

Vehicle Face Re-identification Based on Nonnegative Matrix Factorization with Time Difference Constraint

( Na Ma ),( Tingxin Wen ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.6

Light intensity variation is one of the key factors which affect the accuracy of vehicle face re-identification, so in order to improve the robustness of vehicle face features to light intensity variation, a Nonnegative Matrix Factorization model with the constraint of image acquisition time difference is proposed. First, the original features vectors of all pairs of positive samples which are used for training are placed in two original feature matrices respectively, where the same columns of the two matrices represent the same vehicle; Then, the new features obtained after decomposition are divided into stable and variable features proportionally, where the constraints of intra-class similarity and inter-class difference are imposed on the stable feature, and the constraint of image acquisition time difference is imposed on the variable feature; At last, vehicle face matching is achieved through calculating the cosine distance of stable features. Experimental results show that the average False Reject Rate and the average False Accept Rate of the proposed algorithm can be reduced to 0.14 and 0.11 respectively on five different datasets, and even sometimes under the large difference of light intensities, the vehicle face image can be still recognized accurately, which verifies that the extracted features have good robustness to light variation.

Role of phospholipase D1 in glucose-induced insulin secretion in pancreatic ${\beta}$ cells

Ma, Wei-Na,Park, Shin-Young,Han, Joong-Soo Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.6

As glucose is known to induce insulin secretion in pancreatic ${\beta}$ cells, this study investigated the role of a phospholipase D (PLD)-related signaling pathway in insulin secretion caused by high glucose in the pancreatic ${\beta}$-cell line MIN6N8. It was found that the PLD activity and PLD1 expression were both increased by high glucose (33.3 mM) treatment. The dominant negative PLD1 inhibited glucose-induced Beta2 expression, and glucose-induced insulin secretion was blocked by treatment with 1-butanol or PLD1-siRNA. These results suggest that high glucose increased insulin secretion through a PLD1-related pathway. High glucose induced the binding of Arf6 to PLD1. Pretreatment with brefeldin A (BFA), an Arf inhibitor, decreased the PLD activity as well as the insulin secretion. Furthermore, BFA blocked the glucose-induced mTOR and p70S6K activation, while mTOR inhibition with rapamycin attenuated the glucose induced Beta2 expression and insulin secretion. Thus, when taken together, PLD1 would appear to be an important regulator of glucose-induced insulin secretion through an Arf6/PLD1/mTOR/p70S6K/ Beta2 pathway in MIN6N8 cells.

Effective Coupling of a Topological Corner-state Nanocavity to Various Plasmon Nanoantennas

Na Ma,Ping Jiang,You Tao Zeng,Xiao Zhen Qiao,Xian Feng Xu 한국광학회 2022 Current Optics and Photonics Vol.6 No.5

Topological photonic nanocavities are considered to possess outstanding optical performance, and provide new platforms for realizing strong interaction between light and matter, due to their robustness to impurities and defects. Here hybrid plasmonic topological photonic nanocavities are proposed, by embedding various plasmon nanoantennas such as gold nanospheres, cylinders, and rectangles in a topological photonic crystal corner-state nanocavity. The maximum quality factor Q and minimum effective mode volume V eff of these hybrid nanocavities can reach the order of 10 4 and 10 −4 (λ /n) 3 respectively, and the high figures of merit Q/V eff for all of these hybrid nanocavites are stable and on the order of 10 5(λ /n) −3 . The relative positions of the plasmon nanoantennas will influence the coupling strength between the plasmon structures and the topological nanocavity. The hybrid nanocavity with gold nanospheres possesses much higher Q, but relatively large V eff . The presence of a gold rectangular structure can confine more electromagnetic energy within a smaller space, since its V eff is smallest, although Q is lowest among these structures. This work provides an outstanding platform for cavity quantum electrodynamics and has a wide range of applications in topological quantum light sources, such as single-photon sources and nanolasers.

Exploring personalized fashion design process using an emotional data visualization method

Na Ma,Jieun Kim,이지현 한국의류학회 2022 Fashion and Textiles Vol.9 No.1

In recent years, rapid economic growth and a rising personal income have increased the demand for personalized services. To address this demand, the fashion industry and academia are increasingly analyzing and developing methods to provide personalized fashion design products. This study investigated an emotional data and data visualization-based design method for personalized fashion products. By visualizing emotions and involving consumers, we generated experimental designs to encourage interpersonal and emotional communication. In addition, we proposed methods for visualizing 28 levels of emotion in design elements, as well as a generative design process based on emotional and personal text messages. In fashion products, we used color and print to match the emotion and intensity of the emotion. As a result, 40 design experiment participants rated personalized fashion design tools, outcomes, and purchase intention positively. The highest score was received by the average value for expression of personality (4.43), purchase intention (4.38), and preference and recommendation (4.42). Consequently, this study could be applied to the use of personal data in generative fashion design, as well as the systemization of the data-driven design method for personalized and participatory fashion design.

Chloride Diffusivity of High-Performance Concrete due to Early-Age Shrinkage Cracking

Li-Na Ma,Yanhua Zhao,Jinxin Gong 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.12

Due to the addition of mineral admixtures, early-age shrinkage cracking is a common feature in high-performance concrete (HPC). Chloride diffusivity of HPC due to early-age shrinkage cracking was investigated through rapid chloride migration (RCM) method. Restrained/unrestrained slabs made of HPC containing fly ash (FA) and ground granulated blast-furnace slag (GGBS) were left outdoors for early-age shrinkage cracking, and then cylindrical samples were drilled from slabs for RCM test to quantify the chloride diffusion coefficient, wherein a crack influence factor was introduced to account for the contribution of cracking in the chloride diffusivity progress. Test results from unrestrained HPC reveal that the addition of mineral admixtures could densify the pore structure of HPC thus improved the chloride diffusion coefficient, though FA had a delayed effect. The RCM tests from restrained HPC indicate that the crack indeed had an effect on the chloride ion transportation, but pore structure still dominated the chloride ingress. For a fixed cement replacement, the more the GGBS in the mix, the higher the contribution of cracking to chloride ion penetration.

Role of phospholipase D1 in glucose-induced insulin secretion in pancreatic β cells

Wei-na Ma,한중수,Shin-Young Park 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.6

As glucose is known to induce insulin secretion in pancreatic β cells, this study investigated the role of a phospholipase D (PLD)-related signaling pathway in insulin secretion caused by high glucose in the pancreatic β-cell line MIN6N8. It was found that the PLD activity and PLD1 expression were both increased by high glucose (33.3 mM) treatment. The dominant negative PLD1 inhibited glucose-induced Beta2 expression,and glucose-induced insulin secretion was blocked by treatment with 1-butanol or PLD1-siRNA. These results suggest that high glucose increased insulin secretion through a PLD1-related pathway. High glucose induced the binding of Arf6 to PLD1. Pretreatment with brefeldin A (BFA), an Arf inhibitor, decreased the PLD activity as well as the insulin secretion. Furthermore,BFA blocked the glucose-induced mTOR and p70S6K activation, while mTOR inhibition with rapamycin attenuated the glucose induced Beta2 expression and insulin secretion. Thus, when taken together, PLD1would appear to be an important regulator of glucose-induced insulin secretion through an Arf6/PLD1/mTOR/p70S6K/ Beta2 pathway in MIN6N8cells.