Effect of Fluorescence Light on Phenolic Compounds and Antioxidant Activities of Soybeans (Glycine max L. Merrill) during Germination

Ming Yuan,Xuejing Jia,ChunBang Ding,Haiqiong Zeng,Lei Du,Shu Yuan,Zhongwei Zhang,Qi Wu,Chao Hu,Jing Liu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.5

Effects of light on phenolic compounds and antioxidant activities during germination of soybean seeds were studied. Soybean seeds were germinated in the presence and absence of light. Chlorophylls and morphological characteristics of sprouts were evaluated daily. Antioxidant activities were determined using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ferric reducing activity power methods. Morphological characteristics of light-grown sprouts were superior to dark-grown sprouts and the metabolism of phenolic compounds was regulated by light. Light treatment improved accumulation of phenolic compounds in soybean sprouts and increased antioxidant activities. The optimum harvest time of light-grown sprouts was on the seventh day when sprouts achieved the best nutraceutical value. Green sprouts can serve as a good vegetable source for the human diet.

N-doped porous carbons with increased yield and hierarchical pore structures for supercapacitors derived from an N-containing phenyl-riched copolymer

Ding-Ming Xue,Shi-Chao Qi,Xin Liu,Yu-Xia Li,Xiao-Qin Liu,Lin-Bing Sun 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

N-doped porous carbon-based materials (NPCMs) with hierarchical pore structures have beenconsidered to be a suitable alternative to meet the ever-increasing demands for supercapacitors;however, the universally low yield of the NPCMs has restricted their practical applications. Herein, aseries of NPCMs with hierarchical pore structures are synthesized with significantly increased yieldsthrough the carbonization of the copolymer made from 2,4,6-tris(chloromethyl)mesitylene and pphenylenediamine. The development of the hierarchical pore structures and the N content of the NPCMsshow opposite dependences on the increasing carbonization temperature. The NPCM exhibits the bestcapacitive ability only if the sufficiently developed hierarchical structures and moderate N content areachieved simultaneously. Therefore, NPCM-600 that is carbonized at 600 C with an excellent yield of53.6% (wt.), large specific surface area of 1778 m2 g 1, and N content of 4.13% (wt.) yields an ideal specificcapacitance of 298 F g 1 at the current density of 1 A g 1 and a perfect cycling stability of the capacitanceafter 10,000 cycles at 10 A g 1. The yield of the NPCM-600 is considerably higher than those for manyother recently reported NPCMs. NPCM-600 also shows better capacitance than those of the otherreported NPCMs, such as NOPC-bis-CN-3 (167 F g 1) and CHCPB-K-600 (260 F g 1).

Privet golden leaves adapt unexpectedly well to light changes

Ming Yuan,Bo Huang,Li-Hua Dong,Qiao-Hong Han,Yong Yang,Chun-Bang Ding,Chao Hu,Yang-Er Chen,Zhong-Wei Zhang,Shu Yuan 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.4

Golden-leaf privet ( Ligustrum × vicaryi ) is widely used as a horticultural shrub because of its upper golden leaves, butits lower leaves are green. However, the putative mechanisms of its upper golden leaves and the leaf color changes inresponse to light shifts have not been well studied so far. Here, chlorophylls (Chl), carotenoids, and Chl precursors from bothgolden and green leaves grown in full sunlight (approximately 1200 μmol photons m −2 s −1 at noon) or low-light conditions(180 μmol m −2 s −1 ) were determined spectrophotometrically. In addition, their gas exchange parameters and Chl fl uorescencewere measured in situ. Metabolic fl ux analysis of chlorophyll intermediates indicated that the conversion of prochlorophyllideto chlorophyllide was signifi cantly blocked in golden leaves when compared with green leaves. Green leaves showed higherphotosynthetic capacity in low light than golden leaves, but golden leaves presented unexpectedly stronger photosyntheticcapacity and lower reactive oxygen species accumulation under the high-light condition. Furthermore, golden leaves showeda higher level of nonphotochemical quenching (NPQ) after the light-to-dark shift and presented a stronger adaptive abilityto a broad range of light environments. Higher NPQ values and less oxidative damage in golden leaves may be correlatedwith their higher carotenoid levels. The results imply that lower chlorophyll levels and higher carotenoid levels in canopyleaves may help privet plants acclimate better to illumination changes. This study demonstrates the key role of irradiance ingenerating the two types of Ligustrum × vicaryi leaves and sheds a light on cultivation of other ornamental foliage plants.

A Novel Method for Unstable-signal Sensor Localization in Smart Home Environments

Chao-Chun Chen,Ding-Chau Wang,Yong-Ming Huang 보안공학연구지원센터 2008 International Journal of Smart Home Vol.2 No.3

Wireless sensor networks receive much attention in these few years due to its wide spectrum of applications. Localization is one of the significant techniques in the ubiquitous sensor networks, and most localization techniques nowadays apply RSSI-based ranging techniques to compute the location of the object in wireless sensor network. However, a wireless sensor network is of a fading-signal environment that comprises noises, and the noises cause RSSI to become unstable and lead to abrupt distance estimates. In this paper, we propose Verification-Based Localization Method (VBLM) to alleviate the effect of the unstable signal and provide the high-accuracy location estimates in wireless sensor environments. The basic idea of VBLM is to prune noisy signals in the localization process. In our method, the sensor node would use another neighboring beacon to assist to verify the quality of received signal under acceptable communication cost, and thus, the noisy signals can be removed to avoid increasing the error in the localization. The experimental results show that VBLM indeed reduces the localization error in the unstable signal sensor networks than other localization methods.

Microwave Ablation Treatment of Liver Cancer with a 2,450-MHz Cooled-shaft Antenna: Pilot Study on Safety and Efficacy

Jiao, De-Chao,Zhou, Qi,Han, Xin-Wei,Wang, Ya-Feng,Wu, Gang,Ren, Jian-Zhuang,Wang, Yan-Li,Ding, Peng-Xu,Ma, Ji,Fu, Ming-Ti Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

To evaluate efficacy of microwave ablation in a primary clinical study, sixty patients (44 men, 16 women; mean age 53 years) with 96, 1-8 cm (mean $3.20{\pm}0.17$ cm) liver cancers were treated with 2,450-MHz internally cooled-shaft antenna. Complete ablation (CA) and local tumor progression (LTP) rates as well as complications were determined. CA rates in small (< 3.0 cm), intermediate (3.1-5.0 cm) and large (5.1-8.0 cm) liver cancers were 96.4% (54/56), 92.3% (24/26) and 78.6% (11/14), respectively. During a mean follow-up period of $17.17{\pm}6.52$ months, LTP occurred in five (5.21%) treated cases. There was no significant difference in the CA and LTP rates between the HCC and liver metastasis patient subgroups (P<0.05). Microwave ablation provides a reliable, efficient, and safe technique to perform hepatic tumor ablation.

Poster Session:PS 0235 ; Gastroenterology : Hadha Plays a Role of Double-Edged Sword in Hepatic Steatosis and Cell Injury in Nonalcoholic Fatty Liver Disease

( Jie Xia Ding ),( Meng Li ),( Xing Yong Wan ),( Xi Jin ),( Shao Hua Chen ),( Chao Hui Yu ),( You Ming Li ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: The mechanisms of pathogenesis underlying nonalcoholic fatty liver disease (NAFLD) remain unclear at present and the depth study of HADHA in the development of NAFLD has never been investigated. Methods: The NAFLD cell model was established by treating L02 cells with free fatty acid (FFA) overload. Small interfering RNA (siRNA) was used to knock down HADHA level. The expression of HADHA and key enzymes associated with fatty acid beta- oxidation in L02 cells were determined by q-PCR. Key protein associated in energy metabolism, endoplasmic reticulum(ER) stress and infl ammatory were determined by western blotting. ATP, hydroperoxide (H2O2), catalase (CAT) and mitochondrial membrane potential (MMP) were measured. The prediction of HADHA upstream regulation of miRNA was carried out and luciferase reporter assays were implemented to validate the prediction. Results: After culturing L02 cells by FFA for 48h, we detect the increased protein level of HADHA. HADHA knockdown in L02 cells resulted in an increased of lipid accumulation and downregulation of gene expression involved in fatty acid beta-oxidation, including PPARa, ACOX1, CPT2, EHHADH, ECHS1, HADHB and HADH. Additionally, administering HADHA siRNA exhibited improvement of oxidative stress, embodied in decreased level of H2O2 and MDA, meanwhile, increased levels of ATP, CAT and MMP. Furthermore, HADHA knockdown demonstrated weakened AMPK pathway, activation of MAPK and MKK3 pathway, and improve ER stress by downregulation of C/EBPa and C/EBPß. Moreover, HADHA was regulated directly by upstream gene of miR-124. Conclusions: Our results show that HADHA may plays a role of double-edged sword in hepatic steatosis and cell injury in NAFLD, and provide a new insight into the pathogenic mechanisms of NAFLD, may becoming a potential new therapeutic target for NAFLD.

Poster Session:PS 0234 ; Gastroenterology : Effect of Mir-34a in Regulating Steatosis by Targeting PPARa Expression in NAFLD

( Jie Xia Ding ),( Meng Li ),( Xi Jin ),( Shao Hua Chen ),( Chao Hui Yu ),( You Ming Li ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: The association between altered expression of miR-34a and pathophysiological features of nonalcoholic fatty liver disease (NAFLD) and whether there is a connection between susceptibility to NAFLD has not been completely clarifi ed. Methods: The vitro model was established by culturing L02 cells with a high concentration of free fatty acid (FFA) and the vivo model was established by feeding C57BL/6 mice with HFD. To determine the effects of miR-34a, cultured L02 cells transfected with miR-34a inhibitor and C57BL/6 mice injected with miR-34a inhibitor through vein tail were analysed for the level of PPARaand the metabolic sensor AMPK. In functional experiments, TG content and steatosis degree were measured by TG assay kit, HE and Oil Red O staining. Results: miR-34a expression is signifi cantly upregulated in steatosis-induced hepatocytes and in liver tissues of HFD fed mice. The upregulation of miR-34a resulted in the downregulation of hepatic peroxisome proliferator-activated receptor-a(PPARa), the direct target of miR-34a. Moreover, the action of miRNA-34a on PPAR-a depends on the presence of a single miRNA-34a binding site. Silencing miR-34a led to an initial increase the level of PPARaand the targets of PPARa, including CPT1, CPT2, SLC27A4, SLC27A1 and ACBD3. Activation of the central metabolic sensor AMPK was also increased. In functional experiments, miR-34a inihibitor suppressed cell and mice liver TG content and improve steatosis degree. Furthermore, inhibition of PPARa expression aggravated hepatocellular steatosis in vitro models. Conclusions: NAFLD is associated with altered hepatic miR-34a expression. Decreased expression of miR-34a potentially contributes to altered lipid metabolism implicated in the pathogenesis of NAFLD. These results suggest that regulating it`s target PPARa by down-regulation of miR-34a levels may be a therapeutic strategy against NAFLD.

Prevalence and Risk Factors of Postdialysis Fatigue in Patients Under Maintenance Hemodialysis: A Systematic Review and Meta-Analysis

You Qian,Bai Ding-xi,Wu Chen-xi,Chen Huan,Hou Chao-ming,Gao Jing 한국간호과학회 2022 Asian Nursing Research Vol.16 No.5

Purpose: Despite the high prevalence of postdialysis fatigue (PDF) in maintenance hemodialysis patients, no meta-analysis on the prevalence and risk factors of PDF has yet been published. This study aimed to identify the prevalence of PDF and explore its related factors. Methods: PubMed, Embase, CENTRAL, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL), and the four Chinese databases (National Knowledge Infrastructure [CNKI], Chinese Biomedical Literature database [SinoMed], Wanfang Digital Periodicals [WANFANG], and Chinese Science and Technology Periodicals [VIP] database) were searched from inception up to July 2022. This study was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines. The articles were independently searched by two reviewers, and the relevant data were extracted. The Agency for Healthcare Research and Quality was used to assess the quality of the included studies. Results: Thirteen articles with 2,118 participants were included. The pooled prevalence was 60.0%. The meta-analysis results revealed that the ultrafiltration volume, mean arterial pressure after dialysis, and good sleep quality were potentially associated with PDF, whereas only good sleep quality (odds ratio 0.24, 95% confidence interval 0.19e0.30) was significantly associated with PDF. Conclusion: PDF is common in maintenance hemodialysis patients, which is related to the ultrafiltration volume, sleep quality, and mean arterial pressure after dialysis. However, the mechanism underlying the risk factors and PDF remains unknown. Further research is warranted to investigate the risk factors, intervention, treatment, and mechanism in maintenance hemodialysis patients.

SD-ICN: Toward Wide Area Deployable Software Defined Information Centric Networking

( Changyou Xing ),( Ke Ding ),( Chao Hu ),( Ming Chen ),( Bo Xu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.5

Information Centric Networking that uses content name instead of IP address as routing identifier can handle challenges such as traffic explosion and user mobility, but it also suffers from scalability and incompatibility problems. In this paper by combining the concept of software defined networking and Internet end to end arguments, we propose a wide area deployable software defined information centric networking service model named SD-ICN. SD-ICN employs a dual space structure that separates edge service network and core transmission network. The enhanced SDN techniques are used in edge service network in order to implement intelligent data routing and caching, while traditional IP technique is reserved in core transmission network so as to provide wide area high speed data transmission. Besides, a distributed name resolution system based on the cooperation of different controllers is also presented. The prototype experiments in our campus network show that SD-ICN can be deployed in a scalable and incremental way with no modification of the core network, and can support typical communication modes such as multicast, mobility, multihoming, load balancing, and multipath data transmission effectively.

Berberine suppresses in vitro migration of human aortic smooth muscle cells through the inhibitions of MMP-2/9, u-PA, AP-1, and NF-κB

( Su Jian Liu ),( Cai Xia Yin ),( Ming Chao Ding ),( Shao You Xia ),( Qin Min Shen ),( Ji Dong Wu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.7

Berberine, a type of isoquinoline alkaloid isolated from Chinese medicinal herbs, has been reported to have various pharmacological activities. Studies have demonstrated that berberine has beneficial effects on vascular remodeling and alleviates restenosis after vascular injury. However, its mechanism of action on vascular smooth muscle cell migration is not fully understood. We therefore investigated the effect of berberine on human aortic smooth muscle cell (HASMC) migration. Boyden chamber assay was performed to show that berberine inhibited HASMC migration dose- dependently. Real-time PCR and Western blotting analyses showed that levels of matrix metalloproteinase (MMP)-2, MMP-9, and urokinase-type plasminogen activator (u-PA) were reduced by berberine at both the mRNA and protein levels. Western blotting assay further confirmed that activities of c-Fos, c-Jun, and NF-κB were significantly attenuated. These results suggest that berberine effectively inhibited HASMC migration, possibly by down-regulating MMP-2, MMP-9, and u-PA; and interrupting AP-1 and NF-κB mediated signaling pathways. [BMB Reports 2014; 47(7): 388-392]