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( Dong Hua Li ),( Young Kyoon Oh ),( Sang Rak Lee ),( Seong Ho Choi ),( Ok Ji Un ),( Yong Joo Seol ),( Whan Gook Nho ),( Sang Ho Moon ),( Kyoung Hoon Kim ) 한국동물자원과학회(구 한국축산학회) 2011 한국축산학회지 Vol.53 No.6
The objectives of experiments were to compare directly the effects of synchrony of slowly fermented(corn and corn gluten meal; C-CGM) and of rapidly fermented ingredients(barley and soybean meal; B-SBM) in the rumen on starch disappearances in gastrointestinal tracts(Experiment 1) and growth performance(Experiment 2) of Hanwoo steers in the feedlot barn. In experiment 1, four Hanwoo steers(288±21kg) fitted with ruminal and "T" shaped duodenal cannula were placed in one pen with Calan gate and assigned randomly to a duplicate 2×2 Latin square design. In experiment 2, eight intact Hanwoo steers(311 ±8kg) were assigned randomly to one of two pens with Calan gate to evaluate the effect of the same diets as like in experiment 1 on growth performance. There were no differences in ruminal pH, ammonia and total VFA concentrations between treatments. Percentage of apparent ruminal starch disappearance was 33.3% unit lower(p<0.05) for steers fed C-CGM than for steers fed B-SBM diets and this difference resulted in 268% higher(p<0.05) in duodenal starch flows for steers fed C-CGM diet than for steers fed B-SMB diet. There was significant increase(p<0.05) in quantity(927 vs. 400 g/d) of corn starch digested post-ruminally compared to barley starch. However, percentage of starch apparently digested post-ruminally was 8% higher(p=0.1) in steers fed fast synchrony diet with B-SBM than in steers fed slow synchrony diets with C-CGM. The differences of percentage and amount of starch apparently digested post-ruminally between C-CGM and B-SBM diets did not affect rice straw DM intake, average daily gain(ADG) and feed efficiency. In conclusion, there is some uncertainty in regards to the relationship between site of starch digestion and DM intake, ADG, and feed efficiency in this study.
Structural health monitoring of innovative civil engineering structures in Mainland China
Li, Hong-Nan,Li, Dong-Sheng,Ren, Liang,Yi, Ting-Hua,Jia, Zi-Guang,LI, Kun-Peng Techno-Press 2016 Structural monitoring and maintenance Vol.3 No.1
This paper describes the backgrounds, motivations and recent history of structural health monitoring (SHM) developments to various types of engineering structures. Extensive applications of SHM technologies in bridges, high-rise buildings, sport avenues, offshore platforms, underground structures, dams, etc. in mainland China are summarily categorized and listed in tables. Sensors used in implementations, their deployment, damage identification strategies if applicable, preliminary monitoring achievements and experience are presented in the lists. Finally, existing problems and promising research efforts in civil SHM are discussed, highlighting challenges and future trends.
Li-Hua Zhu,Gang Li,Zhi-Qian Dong 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.2
The structural damage or collapse caused by weak-story failure mechanisms poses a great threat to the safety of human life and property under strong earthquakes. Many researchers have attempted to transform this unexpected failure mechanism into the desired overall failure mechanism by installing various energy dissipation devices on unsafe structures. This paper introduced a lattice-shaped friction device (LSFD), which is a friction device with hardening postyielding stiffness, into a steel frame with a weak-story failure mechanism. Then, shaking table tests of a three types of two-story steel frames—a frame with LSFDs, a frame with traditional friction brace dampers (FBDs), and a bare frame—were carried out. The seismic responses of the hardening postyielding stiffness of the LSFD on the weak-story failure mechanism of the frame were emphatically studied. The results showed that there was little difference in the seismic responses between the two damped structures under moderate and weak earthquakes. The distribution of maximum story drift for the structure with LSFDs was more uniform, which effectively suppressed the weak-story failure under strong earthquakes, whereas the structure with FBDs had serious deformation concentrations. The numerical simulation results of the structure with LSFDs in the shaking table test showed that the simplified model results were basically consistent with the experimental results. Hence, this model could be used to analyze the seismic performance of damped structures with LSFDs.
Citricoccus alkalitolerans sp. nov., a novel actinobacterium isolated from a desert soil in Egypt
Li, Wen-Jun,Chen, Hua-Hong,Zhang, Yu-Qin,Kim, Chang-Jin,Park, Dong-Jin,Lee, Jae-Chan,Xu, Li-Hua,Jiang, Cheng-Lin Microbiology Society 2005 International journal of systematic and evolutiona Vol.55 No.1
<P>An actinobacterium, strain YIM 70010<SUP>T</SUP>, which was isolated from a desert soil sample collected in Egypt, was subjected to a polyphasic taxonomy study. The organism was alkalitolerant and its optimum growth occurred at pH 8·0-9·0. The isolate contained chemotaxonomic markers that were characteristic of the genus <I>Citricoccus</I>, i.e. the peptidoglycan type Lys-Gly-Glu (variation A4<I>α</I>), the predominant menaquinone MK-9(H2) and a polar lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unknown glycolipids. The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The G+C content of the genomic DNA was 63·8 mol%. Strain YIM 70010<SUP>T</SUP> exhibited a 16S rRNA gene sequence similarity of 99·6 % and DNA-DNA relatedness value of 56 % with <I>Citricoccus muralis</I> DSM 14442<SUP>T</SUP>. The phenotypic characteristics and DNA-DNA relatedness data indicate that strain YIM 70010<SUP>T</SUP> can be distinguished from <I>C. muralis</I> (DSM 14442<SUP>T</SUP>). Therefore, on the basis of the polyphasic taxonomic data presented, a novel species of the genus <I>Citricoccus</I>, <I>Citricoccus alkalitolerans</I> sp. nov. (type strain, YIM 70010<SUP>T</SUP>=CCTCC AA 203008<SUP>T</SUP>=DSM 15665<SUP>T</SUP>=KCTC 19012<SUP>T</SUP>) is proposed.</P>
Li, Jun-Qin,Xue, Hui,Zhou, Lan,Dong, Li-Hua,Wei, Da-Peng,Li, Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18
Objective: The mechanism of action of fatty acid synthase (FASN) in drug tolerance of breast cancer cells with epithelial-mesenchymal transition (EMT) features was investigated. Methods: The breast cancer cell line MCF-7-MEK5 with stably occurring EMT and tumour necrosis factor-${\alpha}$ (TNF-${\alpha}$) tolerance was used as the experimental model, whereas MCF-7 acted as the control. Tumour cells were implanted into nude mice for in vivo analysis, and cerulenin was used as a FASN inhibitor. RT-PCR, real-time quantitative PCR and Western blot were employed to detect the expression of FASN, TNFR-1, TNFR-2, Wnt-1, ${\beta}$-catenin and cytC at the RNA and protein levels. Results: Compared with MCF-7, TNFR-1 expression in MCF-7-MEK5 was slightly changed, TNFR-2 was decreased, and FASN, Wnt-1, ${\beta}$-catenin and cytC were increased. The expression of Wnt-1 and ${\beta}$-catenin in MCF-7-MEK5 decreased after cerulenin treatment, whereas cytC expression increased. Conclusions: The important function of FASN in the drug tolerance of breast cancer may be due to the following mechanisms: FASN downregulated TNFR-2 expression through lipid rafts to make the cells less sensitive to TNF-${\alpha}$, and simultaneously activated the Wnt-$1/{\beta}$-catenin signalling pathway. Thus, cytC expression increased, which provided cells with anti-apoptotic capacity and induced drug tolerance.
Dong-Ju Chen,Li-Hua Yan,Qian Li,Cai-jiao Zhang,Chuan-Ling Si,Zhong-Yuan Li,Ya-Jian Song,Hao Zhou,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.6
Conjugated linoleic acid (CLA) isomers, c9, t11- CLA and t10, c12-CLA, have been proved to exhibit excellent biomedical properties for potential use in anticancer applications and in reducing obesity. Acer truncatum Bunge (ATB), which is rich in unsaturated fatty acids, including oleic acid, linoleic acid, and nervonic acid, is a new resource for edible oil. In the present study, we developed a new method for producing two CLA isomers from ATB-seed oil by fermentation using Lactobacillus plantarum CGMCC8198 (LP8198), a novel probiotics strain. Polymerase chain reaction results showed that there was a conserved linoleate isomerase (LIase) gene in LP8198, and its transcription could be induced by ATBseed oil. Analyses by gas chromatography–mass spectrometry showed that the concentration of c9, t11-CLA and t10, c12-CLA in ATB-seed oil could be increased by about 9- and 2.25-fold, respectively, after being fermented by LP8198.
Li, Wen-Jun,Kroppenstedt, Reiner M.,Wang, Dong,Tang, Shu-Kun,Lee, Jae-Chan,Park, Dong-Jin,Kim, Chang-Jin,Xu, Li-Hua,Jiang, Cheng-Lin Microbiology Society 2006 International journal of systematic and evolutiona Vol.56 No.5
<P>Five novel Nocardiopsis strains isolated from hypersaline soils in China were subjected to a polyphasic analysis to determine their taxonomic position. All of the novel isolates could grow on agar plates at NaCl concentrations of up to 18 % (w/v), with optimum growth at 5-8 %. The DNA G+C contents of the novel strains ranged from 67.9 to 73.2 mol%. The morphological and chemotaxonomic characteristics of the isolates matched those described for members of the genus Nocardiopsis. Based on their 16S rRNA gene sequence analysis, DNA-DNA hybridization values and phenotypic characteristics, including the composition of cell-wall amino acids and sugars, menaquinones, polar lipids and cellular fatty acids, the isolates are proposed as representing five novel species of the genus Nocardiopsis. The novel species are proposed as Nocardiopsis gilva sp. nov. [type strain YIM 90087T (=KCTC 19006T=CCTCC AA 2040012T=DSM 44841T)], Nocardiopsis rosea sp. nov. [type strain YIM 90094T (=KCTC 19007T=CCTCC AA 2040013T=DSM 44842T), Nocardiopsis rhodophaea sp. nov. [type strain YIM 90096T (=KCTC 19049T=CCTCC AA 2040014T=DSM 44843T), Nocardiopsis chromatogenes sp. nov. [type strain YIM 90109T (=KCTC 19008T=CCTCC AA 2040015T=DSM 44844T) and Nocardiopsis baichengensis sp. nov. [type strain YIM 90130T (=KCTC 19009T=CCTCC AA 2040016T=DSM 44845T). On the basis of the chemotaxonomic data, the description of the recently described species Nocardiopsis salina Li et al. 2004 is emended.</P>
Micrococcus endophyticus sp. nov., isolated from surface-sterilized Aquilaria sinensis roots.
Chen, Hua-Hong,Zhao, Guo-Zhen,Park, Dong-Jin,Zhang, Yu-Qin,Xu, Li-Hua,Lee, Jae-Chan,Kim, Chang-Jin,Li, Wen-Jun Society for General Microbiology 2009 International journal of systematic and evolutiona Vol.59 No.5
<P>A Gram-positive bacterial strain, designated YIM 56238(T), was isolated from plant roots (Aquilaria sinensis), and characterized by using a polyphasic approach. Strain YIM 56238(T) grew optimally at pH 7.0-8.0 and at 28 degrees C. Analysis of the 16S rRNA gene sequence of strain YIM 56238(T) indicated that it belongs to the genus Micrococcus. Chemotaxonomic data strongly supported the classification of this strain within the genus Micrococcus: the cell-wall peptidoglycan contained lysine, glutamic acid, alanine and glycine; the predominant menaquinones were MK-8(H(2)) (63.6 %) and MK-7(H(2)) (21.1 %); the phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown ninhydrin-negative phospholipid; and the major cellular fatty acids were iso-C(15 : 0) (30.95 %) and anteiso-C(15 : 0) (53.75 %). The G+C content of the genomic DNA was 72.9 mol%. A number of physiological features were found that clearly distinguished strain YIM 56238(T) from recognized species of the genus Micrococcus. DNA-DNA hybridization studies suggested that the novel strain represents a separate genomic species. On the basis of the data, therefore, strain YIM 56238(T) represents a novel species of the genus Micrococcus, for which the name Micrococcus endophyticus sp. nov. is proposed. The type strain is YIM 56238(T) (=DSM 17945(T)=KCTC 19156(T)).</P>
Highly Efficient Microwave-assisted Aminolysis of Epoxides in Water
Zuo, Hua,Li, Zhu-Bo,Zhao, Bao-Xiang,Miao, Jun-Ying,Meng, Li-Juan,Jang, Ki-Wan,Ahn, Chul-Jin,Lee, Dong-Ha,Shin, Dong-Soo Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.suppl8
Highly efficient and rapid aminolysis of epoxides with various amines in water under microwave irradiation in the absence of catalyst was developed. Chiral ${\beta}$-amino alcohols were formed in a short time with excellent yields.