Identification and Characterization of a Bacteriocin Produced by an Isolated Bacillus sp. SW1-1 that Exhibits Antibacterial Activity against Fish Pathogens

Kim, Young-Ok,Park, In-Suk,Kim, Dae-Jung,Nam, Bo-Hye,Kim, Dong-Gyun,Jee, Young-Ju,An, Cheul-Min The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5

The selected isolate, Bacillus sp. SW1-1 showed antibacterial activity against both Gram-positive and Gram-negative bacteria involved in fish diseases, including Edwardsiella tarda, Streptococcus iniae, S. parauberis, Vibrio anguillarum, and V. harveyi. The Maximum bacteriocin production was observed at $30^{\circ}C$ after 24 h with brain heart infusion medium (pH 7.0). The bacteriocin SW1-1 was purified by 50% ammonium sulfate precipitation, followed by HiPrep diethylaminoethyl 16/10 FF and Sephacryl S-100 High resolution column chromatography. The substance was characterized as a bacteriocin-like inhibitory substance with a molecular mass of 38 kDa. Bacteriocin SW1-1 was sensitive to the proteolytic action of pepsin, trypsin, chymotrypsin, and protease types I and XIV, and relatively heat labile, despite the fact that bacteriocin activity was still detected after heating at $100^{\circ}C$ for 30 min. The activity of bacteriocin SW1-1 was stable in the pH range of 2.0-11.0, and relatively unaffected by organic chemicals. The bacteriocin SW1-1 had a bacteriolytic mechanism, resulting in cell wall degradation of E. tarda. These characteristics indicate that this bacteriocin may be a potential candidate for alternative agent to control important pathogens of fish diseases in aquaculture.

Determining the Optimal Cooking Time for Cooking Loss, Shear Force, and Off-Odor Reduction of Pork Large Intestines

Sung-Su Kim,Yee Eun Lee,ho Hyun Kim,Joong-Seok Min,Dong Gyun Yim,Cheorun Jo 한국축산식품학회 2022 한국축산식품학회지 Vol.42 No.1

The objective of this study was to determine the optimal cooking time by considering the cooking loss, shear force, and off-odor reduction of pork large intestines. Commercial pork large intestines were purchased, quartered perpendicularly, and cooked in boiling water for 40, 120, 180, and 240 min. Cooking loss of the samples increased after 240 min of cooking (10.92, p<0.05) while shear force value was lower at 240 min (4.45) compared to that at other cooking times (p<0.001). The amount of major volatile organic compounds showed a decreasing trend with increasing cooking time. In particular, the amount of methyl pentanoate (17,528.71) and methyl isobutyrate (812.51), compounds with a relatively low odor threshold, decreased significantly after 120 min of cooking and no change was observed thereafter (p<0.05). In addition, the amount of 2-pentanol (3,785.65) and 1-propanol (622.26), possibly produced by lipid oxidation, significantly decreased at the same cooking time (p<0.001). In the principal component analysis, only the 40 min cooking time was significantly different from other cooking time by high amounts of 1-propanol, 2-pentanol, and methyl isobutyrate. In conclusion, in the present study, the optimal cooking time for pork large intestines was 120 min in terms of off-odor reduction, cooking loss, and shear force.

부유형 해양 광생물반응기의 선택적 투과막의 술폰화 반응을 통한 Biofouling 억제 및 미세조류 생산성 향상

김광민(Kwangmin Kim),이윤우(Yunwoo Lee),김지훈(Z-Hun Kim),박한울(Hanwool Park),정인재(Injae Jung),박재훈(Jaehoon Park),임상민(Sang-Min Lim),이철균(Choul-Gyun Lee) 한국해양바이오학회 2017 한국해양바이오학회지 Vol.9 No.1

The purpose of this study was to inhibit biofouling on a selectively permeable membrane (SPM) and increase biomass productivity in marine photobioreactors (PBRs) for microalgal cultivation by chemical treatment. Surfaces of a SPM, composed of polyethylene terephthalate (PET), was sulfonated to decrease hydrophobicity through attaching negatively charged sulfonic groups. Reaction time of sulfonation was varied from 0 min to 60 min. As the reaction time increased, the water contact angle value of SPM surface was decreased from 75.5o to 44.5o, indicating decrease of surface hydrophobicity. Furthermore, the water permeability of sulfonated SPM was increased from 5.42 mL/㎡/s to 10.58 mL/㎡/s, which reflects higher nutrients transfer rates through the membranes, due to decreased hydrophobicity. When cultivating Tetraselmis sp. using 100-mL floating PBRs with sulfonated SPMs, biomass productivity was improved by 34% compared with the control group (non-reacted SPMs). In addition, scanning electron microscopic observation of SPMs used for cultivation clearly revealed lower degree of cell attachment on the sulfonated SPMs. These results suggest that sulfornation of a PET SPM could improve microalgal biomass productivity by increasing nutrients transfer rates and inhibiting biofouling by algal cells.

간경변 환자에서 Oro-cecal Transit Time의 측정

원경준,이준,변유미,조민근,조주연,서정균,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background: Neurotransmitter like substances in the gut have been proposed to contribute to the overall portal systemic encephalopathy (PSE). The source of the ammonia in patients with PSE is mainly the small bowel, as a result of the conversion of glutamine in the diet to glutamate and ammonia. PSE is highly responsive to changes in diet, to antibiotic therapy and to ingestion of nondigestible disaccharides such as lactulose. This study was performed to evaluate the relationship between PSE and oro-cecal transit time. In addition, performed to evaluate the effect of severity, cause, complications of liver cirrhosis on oro-cecal transit time. Methods: Between August 2006 and February 2007, 48 cirrhotic patients consecutively was enrolled in study their oro-cecal transit time after informed consent was obtained. A non-invasive hydrogen breath test was used to study oro-cecal transit time (OCTT). Result: The OCTT were delayed in cirrhotic patients with PSE compared without PSE (123.3 ± 42.7 min vs 170.0 ± 58.3 min, p<0.05). OCTT tended to prolongate further as the damage in the liver got worse, nevertheless the result was statistically meaningless (p<0.111). Neither presence of ascites or cause of PSE had any influence on gastrointestinal transit. Conclusions: Cirrhotic patients with PSE have more delayed OCTT than without PSE. Further research is needed to find out the relationship between OCTT and severity of liver cirrhosis.

첨단시설원예 경영체의 정보기술 활용능력의 비교 분석

김사균,최영찬,이민수 한국농업정보과학회 2003 농업정보과학 Vol.4 No.1

정보활용능력이 우수한 경영체 집단을 "우수"라 칭하고, 정보활용능력이 우수하지 못한 집단을 "열위"라 명명하였을 때 두 집단간의 생산구조, 생산기술, 경영성과 등 여러 가지 부문에서 대별되었다. 그 연구결과를 요약하면 다음과 같다. 1) 경영규모별로 정보활용능력의 차이검정(분산분석)의 결과 경영규모별로 정보활용능력의 유의한 결과를 보였다. 시설유형에서 유리온실과 경질판 온실간 유의한 차이를 보였으며, 재배양식별로는 양액재배 경영체와 토경재배 경영체 간에도 유의한 차이를 보였다. 2) 우수경영체가 열위경영체에 비해 경영규모의 측면에서 두배 이상 크고, 학력에서도 전문대 이상 학력을 소지한 경영체 수가 두 배 가량 많았으며, 경영기록도 충실히 잘 수행하였다. 생산기술 부문에서 수확기간 및 가온기간이 길고, 온실 이용기간이 긴 것으로 나타났다. 3) 첨단시설원예 경영체의 정보화 능력 수준별 경영성과를 살펴보면, 정보화 능력이 우수한 집단은 열위한 집단보다 10a당 조수입액은 2.2배, 수출액은 2.8배 많았다. 경영성과 만족도의 측면에서도 경영성과에 만족한다는 응답자의 비율이 40%에 달한 반면 열위그룹의 경영성과 만족도는 12%에 그쳤다. 이러한 결과로 미루어 볼 때 정보활용능력과 경영성과의 관계는 매우 밀접한 관계에 있으며, 정보화능력이 우수한 집단일수록 경영성과가 높다고 할 수 있을 것이다. Information literacy and computer literacy is essential in problem solving for farm management. This study surveyed 552 farm of High-tech Greenhouse in South Korea, and examined how much the farmer of High-tech Greenhouse is literate in the information, and how much the management performance is affected by information literacy. The finding of this study show that the management performance is strongly influenced by information literacy of farmer. The superior information literacy farms return more double income than the inferior information literacy farms do. The export sales of the superior information literacy farms is 2.8 times as much as that of the inferior information literacy farms. The 40% of the superior information literacy farms was satisfied with management performance, but only 12% of the inferior information literacy farms was satisfied with management performance.

반응성 DC sputtering법으로 제조한 AIN박막의 수소첨가효과

김지균,김민석,권정열,이헌용 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

In GaAs devices, the development of a finer insulator instead of SiO₂to be used the insulator of Si has been made good progress. That, however, is not to be enough. We studied the development of the finer insulator in GaAs devices. The AIN has the characteristics of thermal stability over 2400℃ and resistivity over 10 13 Ω, and superior insulator characteristic to have rate of thermal expansion(a=4.2x10 -6, b=5.3x10 -6/K) similar with SiO₂and GaAs. An AIN has been evaluated good applied insulator for above characteristics in chemical compound. Then, the purpose of this paper is a development of MIS devices using AIN as an insulator, and is to investigate a electrical characteristic changing by H₂addition to AIN. The ratio of H₂addition was established by 5%. The pressure was maintained by 5 mTorr and the DC power was supplied by 150W during the experiment. The H₂addition to AIN may have an effect on diminishing impurities on surface and improves the properties as an insulator to a MIS structures.

700MHZ대역의 마이크로파 적층 스트립라인 칩 대역통과 필터설계 및 제작

김민석,김지균,이헌용 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

In this thesis, we studied the design and fabrication of microwave multilayer chip filter using by coupled stripline resonator. The microwave multilayer chip filter operating at 700[MHz] is fabricated and designed. The size of microwave multilayer chip filter is 4.5×4.4×1.8[mm]. Insertion loss and center frequency of the fabricated chip filter are 2.58[dB] and 692.5[MHz] respectively. The center frequency was lower 7.5[MHz] than design result, but other characteristics of chip filter were similar to the results of simulation.

산전 초음파로 진단된 선천성 수신증 태아의 경험 1례

강민아,김도균,최석철,양회생,배철성,윤혜원,심재철,하달봉 동국대학교 경주대학 1997 東國論集 Vol.16 No.1

Prenatal ultarsound represents an accurate and reliable method for the detection of intrauterine anomalies, especially urinary tract anomalies. Hydronephrosis is the most common congenital condition detected by prenatal ultrasound. Prenatal ultrasonographic diagnosis allows a good condition and therapeutic assessment of the patients prior to serious impairment of renal function so that in most cases the urological surgeon can opt for a parenchymal conserving procedure. The authors have experienced a case of congenital ureteropelvic obstruction with hydronephrosis diagnosed at 32 weeks of pregnancy and report our experience with a brief review of literature.

N-Arylnortropinone 유도체의 합성연구

李龍均,朴유미,鄭大一,金寅植,朴鍾勳,盧信愛,鄭逸守,朴敏洙 東亞大學校附設基礎科學硏究所 1998 基礎科學硏究論文集 Vol.15 No.1

Tropinone 화합물들은 색다른 고리계의 존재로 아주 오래전부터 약리학적으로 매우 흥미로운 연구과제로 주목되어 최근에 그들의 합성법,?? 입체적 구조분석?? 그리고 약리학적인 연구??가 활발히 진행되고 있다. Tropinone 1은 흔히 마약으로 알려져 있는 cocaine 2, 소염작용과 항경련성 작용 및 심장박동수를 조절 등 다양한 약리작용을 하는 tropane spirohy-dantoin ?? 그리고 안과질환을 검사 할 때 눈의 동공을 확장시키는 데 사용하는 atropine ??등을 합성하는 데 중요한 중간체이다. ??????????? Trigo, G, G등 ??은 aliphatic amine과 succinic aldehyde를 acetonedicarboxylic acid와 반응하여 nortropane 유도체를 합성하였으며, 그 후 [3+2] nitrone cycloaddition? 과 [3+4] cyclocoupling? 을 거치는 tropane alkaloid의 새로운 합성법이 개발되었다. 최근 lzquier M. L. 등¹은 acetonedicarboxylic acid를 사용하여 1차 지방족 amine과 succinic aldehyde대신 2,5-dimethoxytetrahydrofuran을 반응시켜 N-alkyl nortropinone 유도체를 합성하였다. 그러나 일반적으로 빙초산 속에서 amine과 2,5-dimethoxytetrahydrofuran을 반응시키면 pyrrole 유도체가 합성되어진다.?? 이러한 사실은 분명히 반응조건(예, 산, 온도)에 따른 다양한 화학이 존재한다고 볼 수 있다. 따라서 우리는 반응조건, 즉 산의 변화 및 온도 변화에 의한 amine과 2,5-dimethoxytetrahydrofuran의 반응을 자세하게 검토하면서 새로운 N-arylnortropinone 유도체를 합성하고자 한다.

Oct4 단백질의 DNA - 결합 영역에 융합된 HIV - 1 integrase

이민전,서진욱,김미라,신차균 中央大學校 遺傳工學硏究所 2002 遺傳工學硏究論集 Vol.15 No.1

Human immunodeficience virus type-1 (HIV-1) integrase (IN) mediates integration of viral DNA into the host cell genome which is an essential step during the life cycle of retroviruses. HIV-1 IN shows four enzymatic activities in vitro: non-specific DNA- binding, endonucleolytic (3'-processing), integration (strand transfer), and disintegration activities. Oct4 as a transcription factor recognizes and binds the specific sequences containing 5' -ATGCAAAT-3'. In order to study the specific binding and enzymatic activities of the integrase protein, we constructed the four different fusion proteins (OctIN1, OctIN2, INKOct1, INKOct1) using different combination of HIV-1 IN and Oct4 protein. The fusion proteins were overexpressed in Escherichia coli and purified by Ni-NTA and SP-sepharose columns. Analysis of the enzymatic activities showed that all the four fusion proteins has detectable endonucleolytic and integration activities, suggesting that the fusion proteins can be used to study derected integration and sequence specific DNA-binding of the integrase fusion protein.